免疫组化清洗液 (PBST)又称免疫组化漂洗缓冲液,可用于免疫组化染色时一抗或二抗孵育后的洗涤,适当的洗涤可以降低背景,增强信噪比。
免疫组化清洗液 (10×PBST)由PBS、适量Tween 20及防腐剂PC300等配制而成,可以通透细胞膜,经本产品处理的细胞样品可以检测细胞内的目的蛋白,包括细胞浆内和细胞核内的目的蛋白,按照每组样品清洗需要10 ml PBST计算,1个包装的免疫组化清洗液 (PBST)可以洗涤50次。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
1、取适量本品,加入9体积的去离子水,即为免疫组化清洗液 (PBST)。
2、孵育完一抗或二抗后,加入适量免疫组化清洗液 (PBST)完全覆盖样本。
3、在摇床上轻轻摇动漂洗5 min,换液后再重复2次,每次5 min。
注意事项:
1、如果摇动容易导致样本脱落,可以浸泡于该洗涤液中,无需摇动漂洗,但应延长漂洗时间至5-8 min,并增加洗涤次数1-2次。如果背景仍高,可继续增加漂洗时间和次数。
2、一经开启,尽快用完,储存过久的液体效果易下降。
3、一般延长洗涤时间或增加洗涤次数不会对免疫染色结果产生负面影响。
4、试剂开封后请尽快使用,以防影响后续实验效果。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Immunohistochemistry Washing Solution (PBST), also known as Immunohistochemistry Rinsing Buffer, is applicable for washing after the incubation of primary or secondary antibodies during immunohistochemical staining. Proper washing can reduce background interference and enhance the signal-to-noise ratio.
Immunohistochemistry Washing Solution (10×PBST) is formulated with PBS, an appropriate amount of Tween 20 and preservative PC300, which can permeabilize cell membranes. Cell samples treated with this product allow for the detection of intracellular target proteins, including those in the cytoplasm and nucleus. Based on the dosage of 10 ml PBST required for washing per sample group, one package of Immunohistochemistry Washing Solution (PBST) can be used for 50 washes. This reagent is for research use only (RUO) and not intended for clinical diagnosis or any other applications.
Operating Procedures (For Reference Only)
1. Take an appropriate amount of this product and add 9 volumes of deionized water to prepare the working solution of Immunohistochemistry Washing Solution (PBST).
2. After the incubation of primary or secondary antibodies, add an appropriate amount of Immunohistochemistry Washing Solution (PBST) to completely cover the samples.
3. Gently shake and rinse on a shaker for 5 minutes, then change the solution and repeat the rinsing twice, 5 minutes each time.
Precautions
1. If shaking is likely to cause sample detachment, the samples can be soaked in the washing solution without shaking; in this case, extend the rinsing time to 5-8 minutes and increase the rinsing frequency by 1-2 times. If the background interference remains high, the rinsing time and frequency can be further increased.
2. Use the product as soon as possible after opening, as the efficacy of the solution may decrease upon prolonged storage.
3. Generally, extending the washing time or increasing the washing frequency will not exert a negative effect on the results of immunostaining.
4. Use the reagent immediately after opening to prevent adverse impacts on subsequent experimental results.
5. For your safety and health, wear a lab coat and disposable gloves during operation.