计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避免反复冻融。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
| 主要成分 | 含量 |
| 重组人胰岛素 | 0.625 mg/mL |
| 人转铁蛋白 | 0.625 mg/mL |
| 亚硒酸钠 | 0.625 µg/mL |
| 亚油酸 | 0.535 mg/mL |
| 牛血清白蛋白 | 125 mg/mL |
使用方法
推荐稀释浓度1:100(培养时在低血清浓度的培养基中按照比例添加本品)。起初,培养基中必须加入2%-4%的胎牛血清,以便获得与含10%胎牛血清的同种培养基相同的细胞生长效果;经过最初的适应后,胎牛血清浓度可进一步降低。
注意事项
1. 本产品为无菌包装,在使用过程中请注意无菌操作,避免微生物污染。
2. 可放置-20℃延长保存,切忌反复冻融,小量使用时建议分装。
3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
Insulin‑Transferrin‑Selenium‑Linoleic Acid (ITS+, 100×) contains important substances including insulin, transferrin, sodium selenite, and linoleic acid, which are suitable for cell growth and differentiation in vitro. These components promote cell proliferation and reduce the serum concentration required for the maintenance and low-density attachment of various cells. Insulin promotes glucose and amino acid uptake, lipogenesis, phosphate transport, and protein and nucleic acid synthesis. Transferrin, as an iron carrier, helps reduce the toxicity of oxygen free radicals and hydrogen peroxide. Selenite serves as a cofactor for glutathione peroxidase and other proteins, and is used as an antioxidant in the culture medium. Linoleic acid is an essential fatty acid that promotes cell growth, provides antioxidant activity, and enhances the effects of insulin. It has been verified that ITS+ exhibits superior performance compared to ITS in promoting cell proliferation and inducing chondrogenic differentiation of mesenchymal stem cells.
Product Components
| Component | Concentration |
| Recombinant Human Insulin | 0.625 mg/mL |
| Human Transferrin | 0.625 mg/mL |
| Sodium Selenite | 0.625 µg/mL |
| Linoleic Acid | 0.535 mg/mL |
| Bovine Serum Albumin (BSA) | 125 mg/mL |
Instructions for Use
The recommended dilution concentration is 1:100 (add this product to the culture medium containing low serum concentration at the indicated ratio during culture). Initially, 2%-4% fetal bovine serum (FBS) must be added to the medium to achieve the same cell growth effect as the same medium containing 10% FBS. After the initial adaptation period, the FBS concentration can be further reduced.
Precautions
1.This product is supplied sterile. Please ensure aseptic handling during use to avoid microbial contamination.
2.For extended storage, the product may be kept at -20°C. Avoid repeated freeze-thaw cycles. For small volume use, aliquoting is recommended.
3.For your safety and health, please wear a lab coat and disposable gloves when handling.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | I1527936 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
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