概述规格名称和识别符技术文档高级数据系统学分类三维结构质检证书（CoA,COO,BSE/TSE 和分析图谱) 化学和物理性质安全和危险性（GHS）此产品的引用文献技术文档和文章引用文献溶液计算器

🧪

为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

-80℃储存。特低温运输。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 199 篇同行评审文献引用。

概述

IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用，用于重组细菌菌落的蓝白筛选，这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用，防止 β-半乳糖苷酶编码基因 lacZ 的抑制。

IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

规格

英文别名

规格或纯度

10mM in DMSO

英文名称

IPTG (isopropyl-β-D-thiogalactopyranoside)

生化机理

与X-GAL结合使用，用于需要表达β-半乳糖苷酶活性的克隆程序中。半乳糖的不可代谢类似物。

储存条件

-80℃储存

运输条件

特低温运输

名称和识别符

PubChem SID

528764903

EC号

206-703-0

分子类型

小分子

IUPAC Name

(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol

INCHI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChi Key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

Smiles

CC(C)SC1C(C(C(C(O1)CO)O)O)O

Isomeric SMILES

CC(C)S[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O

分子量

238.3

Beilstein号

4631

Reaxy-Rn

11730049

Reaxys-RN link address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=11730049&ln=

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

系统学分类

分类树（Taxonomy Tree）

界（kingdom) 有机化合物
- 超类（Superclass) 有机氧化合物
  - 类（Class) 有机氧化合物
    - 亚类（Subclass）碳水化合物和碳水化合物结合物
      - 第5级 (level 5) 糖基化合物
        第6级 (level 6) 硫代糖苷

界（kingdom)	有机化合物
超类（Superclass)	有机氧化合物
类（Class)	有机氧化合物
亚类（Subclass）	碳水化合物和碳水化合物结合物
中间层级节点（Intermediate Tree Nodes）	糖基化合物
直接上位类(Direct Parent)	硫代糖苷
其他上位类（Alternative Parents）	氧烷单糖单硫缩醛仲醇亚磺酰基化合物多元醇氧环化合物伯醇碳氢化合物衍生物
分子骨架（Molecular Framework)	脂肪族杂单环化合物
取代基（Substituents）	羟基苯甲酸酯 - 脂肪族杂单环化合物 - 烃衍生物 - 单糖 - 单硫缩醛 - 有机杂环化合物 - 有机硫化合物 - 氧杂环 - 氧杂环丁烷 - 多醇 - 初级醇 - S-磺酰基-L-半胱氨酸 - 醛基 - 硫酰基化合物
描述（Description）	该化合物属于有机化合物中的硫苷类。此类苷类通过S-苷键将糖基与另一基团以单碳键相连。
外部描述符（External Descriptors）	S-glycosyl compound

三维结构

交互式化学结构模型

球棍
棍棒
线框模型
空间填充
显示氢气
动画

化学和物理性质

敏感性

对湿度敏感

比旋光度

-32° (C=1,H2O)

熔点

109-121℃

分子量

238.300 g/mol

XLogP3

-0.500

氢键供体数Hydrogen Bond Donor Count

氢键受体数Hydrogen Bond Acceptor Count

可旋转键计数Rotatable Bond Count

精确质量Exact Mass

238.087 Da

单同位素质量Monoisotopic Mass

238.087 Da

拓扑极表面积Topological Polar Surface Area

115.000 Å²

重原子数Heavy Atom Count

形式电荷Formal Charge

复杂度Complexity

201.000

同位素原子数Isotope Atom Count

定义的原子立体中心计数Defined Atom Stereocenter Count

未定义的原子立体中心计数Undefined Atom Stereocenter Count

定义的键立体中心计数Defined Bond Stereocenter Count

未定义的键立体中心计数Undefined Bond Stereocenter Count

所有立体化学键的总数The total count of all stereochemical bonds

共价键合单元计数Covalently-Bonded Unit Count

安全和危险性（GHS）

象形图

GHS07, GHS08

信号词

警告

危险声明

H302: 吞食有害

H312: 皮肤接触有害

H315: 引起皮肤刺激

H319: 引起严重眼睛刺激

H332: 吸入有害

H335: 可能引起呼吸道刺激

H351: 怀疑引起遗传缺陷

预防措施声明

P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾

P264: 处理后要彻底洗手。

P270: 使用本产品时，请勿进食、饮水或吸烟。

P271: 仅在室外或通风良好的地方使用。

P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。

P321: 特殊处理（请参阅此标签上的...）。

P330: 漱口

P302+P352: 如皮肤沾染：用水充分清洗。

P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。

P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。

P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。

P405: 密闭存放

P403+P233: 存放在通风良好的地方。保持容器密闭。

P501: 将内容物/容器处理到。。。

P203: 使用前，获取、阅读并遵守所有安全说明。

P264+P265: 处理后彻底洗手[和…]。不要触摸眼睛。

P301+P317: 如果被吞咽：请寻求医疗帮助。

P318: 如果暴露或担心，请就医。

P317: 寻求紧急医疗救助。

P337+P317: 如果眼睛刺激持续：寻求医疗帮助。

P332+P317: 如果出现皮肤刺激：请寻求医疗帮助。

P319: 如果你感到不适，请寻求医疗帮助。

WGK Germany

Merck Index

5082

个人防护装备

Eyeshields,Gloves,half-mask respirator (EU),half-mask respirator (US),multi-purpose combination respirator cartridge (US)

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到1个结果

批号(Lot Number)	证书类型	货号
E2417013	分析证书	I423647

技术文档和文章

阿拉丁@生物实验常用缓冲液配方指南

目录：技术文章

探索主题:

生物缓冲液电泳生化试剂抗生素 Western blot 染色剂和染料

Aladdin@Guidelines for the formulation of commonly used buffers for biological experiments

目录：技术文章

探索主题:

Western blot Biochemical reagents Antibiotics Stains and dyes Electrophoresis Biological buffers

什么是 IPTG？

目录：技术文章

探索主题:

IPTG LacI 蓝白斑筛选重组蛋白诱导表达定时基因激活

What is IPTG?

目录：技术文章

探索主题:

IPTG LacI Blue-white colony screening Recombinant protein induction Timed gene activation

质粒的五个关键结构特征

目录：技术文章

探索主题:

Plasmids 基因功能解析

Five Key Structural Features of Plasmids

目录：技术文章

探索主题:

质粒 Gene Function Analysis

β-半乳糖苷酶作用机理、结构基础与应用技术指南

目录：技术文章

探索主题:

X-Gal β-半乳糖苷酶乳糖转半乳糖基化低聚半乳糖 lacZ α-互补 SA-β-半乳糖苷酶

β-Galactosidase: Mechanism, Structural Basis, and Application Guide

目录：技术文章

探索主题:

X-Gal lacZ β-galactosidase Lactose Transgalactosylation Galacto-oligosaccharides α-complementation SA-β-galactosidase

多肽合成路线的判断逻辑：从 SPPS、LPPS 到重组表达的适用情形与判断依据

目录：技术文章

探索主题:

多肽合成液相多肽合成多肽化学制备

Decision Logic for Peptide Synthesis Routes: Applicability and Criteria for Choosing among SPPS, LPPS, and Recombinant Expression

目录：技术文章

探索主题:

Peptide synthesis Liquid-phase peptide synthesis

β-半乳糖苷酶作用机理、结构基础与应用技术指南

目录：技术文章

探索主题:

X-Gal β-半乳糖苷酶乳糖转半乳糖基化低聚半乳糖 lacZ α-互补 SA-β-半乳糖苷酶

合成生物学是什么？从上游试剂工具链到生物制造应用的产业化路径

目录：技术文章

探索主题:

合成生物学 SB

What Is Synthetic Biology? The Industrialization Pathway from Upstream Reagent Toolchains to Biomanufacturing Applications

目录：技术文章

探索主题:

SB Synthetic Biology

此产品的引用文献

如何在您的发表作品中引用阿拉丁的产品和网站? 引用文献检索

引用文献

1. Muhammad Ehsan, Rui-Si Hu, Meng Wang, Jun-Ling Hou, Muhammad Rashid, Muhammad Irfan Malik. (2023) Immune modulation of goat monocytes by Fasciola gigantica Legumain-1 protein (Fg-LGMN-1). EXPERIMENTAL PARASITOLOGY, [PMID:38081528] [10.1016/j.exppara.2023.108671]
2. Xinlin Wei, Taijing Zheng, Shunyao Zhu, Xueyuan Liao, Nazi Zhou, Yu Wang, Yue Xu, Meimei Xiong, Rui Liu, Jian Mao, Jie Tao. (2023) Fe3O4-nanoparticle-functionalized 3D-printed injectable microgel. JOURNAL OF APPLIED POLYMER SCIENCE, [10.1002/app.54913]
3. Linlin Xu, Fei Pan, Yingnan Li, Huiqian Liu, Chengtao Wang. (2023) Characterization and Molecular Dynamics Simulation of a Lipase Capable of Improving the Functional Characteristics of an Egg-Yolk-Contaminated Liquid Egg White. Foods, 12 (22): (4098). [PMID:38002155] [10.3390/foods12224098]
4. Gao Yafan, Shen Yu, Fan Jiyuan, Ding Haojie, Zheng Bin, Yu Haijie, Huang Siyang, Kong Qingming, Lv Hangjun, Zhuo Xunhui, Lu Shaohong. (2023) Establishment and application of an iELISA detection method for measuring apical membrane antigen 1 (AMA1) antibodies of Toxoplasma gondii in cats. BMC Veterinary Research, 19 (1): (1-11). [PMID:37924072] [10.1186/s12917-023-03775-1]
5. Xiaoxiang Hu, Wenjing Liu, Yi Yan, Huaxiang Deng, Yujie Cai. (2023) Development of a novel magnetic metal-organic framework for the immobilization of short-chain dehydrogenase for the asymmetric reduction of pro-chiral ketone. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:37838135] [10.1016/j.ijbiomac.2023.127414]
6. Jiang Zhu, Ruinan Qu, Yawei Wang, Ruoyao Ni, Kai Tian, Chan Yang, Mei Li, Michael Kristensen, Xinghui Qiu. (2023) Up-regulation of CYP6G4 mediated by a CncC/maf binding-site-containing insertion confers resistance to multiple classes of insecticides in the house fly Musca domestica. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:37769776] [10.1016/j.ijbiomac.2023.127024]
7. Pei Xiaolin, Xiao Qinjie, Feng Yumin, Chen Li, Yang Fengling, Wang Qiuyan, Li Nanxing, Wang Anming. (2023) Enzymatic properties of a non-classical aldoxime dehydratase capable of producing alkyl and arylalkyl nitriles. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, [PMID:37733049] [10.1007/s00253-023-12767-y]
8. Li Chen, Tang Tiancheng, Jiang Jinju, Yao Zhong, Zhu Benwei. (2023) Biochemical characterization of a new ulvan lyase and its applicability in utilization of ulvan and preparation of ulva oligosaccharides. GLYCOBIOLOGY, [PMID:37593920] [10.1093/glycob/cwad068]
9. Wang Nengqiang, Li Xiaojun. (2023) Mining of a novel reductase and its application for asymmetric reduction of p-methoxyacetophenone. LETTERS IN APPLIED MICROBIOLOGY, 76 (8): [PMID:37533205] [10.1093/lambio/ovad091]
10. Tang Tiancheng, Li Chen, Zhu Benwei, Yao Zhong. (2023) Efficient preparation and production of ulvan oligosaccharides by using a new PL25 family ulvan lyase from Alteromonas sp. 76–1. JOURNAL OF APPLIED PHYCOLOGY, [10.1007/s10811-023-03034-8]
11. Tao Zhang, Bo Li, Chaofeng Wang, Shuilin Wu, Shengli Zhu, Hui Jiang, Yufeng Zheng, Zhaoyang Li, Zhenduo Cui, Yu Zhang, Paul K Chu, Xiangmei Liu. (2023) Tunning the bandgap of MnO2 homojunction by building active high-index facet to achieve rapid electron transfer for enhanced photocatalytic sterilization. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY, [10.1016/j.jmst.2023.05.059]
12. Chenchen Zhao, Yu Sha, Wei Zhuang, Yuan Rao, Jihang Zhang, Jinglan Wu, Tao Shen, Zhuotao Tan, Chenjie Zhu, Hongman Zhang, Hanjie Ying. (2023) Production of hydroxytyrosol from tyrosol via controllable oxidation with immobilized tyrosinase: A comparative study. PROCESS BIOCHEMISTRY, [10.1016/j.procbio.2023.06.017]
13. Jianan Li, Wenjie Huang, Huixia He, Shuangni Shi, Xiuxia Sun, Jianxi Xiao. (2023) Biocompatible and bioactive hydrogels of recombinant fusion elastin with low transition temperature for improved healing of UV-irradiated skin. Journal of Materials Chemistry B, [PMID:37401183] [10.1039/D3TB00564J]
14. Xinyue Ye, Jian Wang, Pengfei Xu, Xiaoqian Yang, Qixue Shi, Genyan Liu, Zhaoshi Bai, Changlin Zhou, Lingman Ma. (2023) Peptide MSI-1 inhibited MCR-1 and regulated outer membrane vesicles to combat immune evasion of Escherichia coli. Microbial Biotechnology, [PMID:37329166] [10.1111/1751-7915.14297]
15. Shijin Wu, Qiang Wang, Xiaojing Ma, Lequan Qiu, Hongde Yan. (2023) Modulation of the catalytic performance of OYE3 by engineering key residues at the entrance of the catalytic pocket. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, [PMID:37073879] [10.1002/bab.2468]
16. Pan Yunjun, Li Guobang, Liu Ruxin, Guo Jiawei, Liu Yunjie, Liu Mingyu, Zhang Xingwang, Chi Luping, Xu Kangwei, Wu Ruibo, Zhang Yuzhong, Li Yuezhong, Gao Xiang, Li Shengying. (2023) Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids. Nature Communications, 14 (1): (1-14). [PMID:36966128] [10.1038/s41467-023-37288-0]
17. Weijun Leng, Xiaoyun Wu, Xianghui Qi, Hongying Liu, Li Yuan, Ruichang Gao. (2023) Systematic functional analysis and potential application of a serine protease from cold-adapted Planococcus bacterium. Food Science and Human Wellness, [10.1016/j.fshw.2023.02.025]
18. Wang Yushang, Wang Lihua, Sun Tianyong, Shen Song, Li Zixuan, Ma Xiaomei, Gu Xiufeng, Zhang Xiumei, Peng Ai, Xu Xin, Feng Qiang. (2023) Study of the inflammatory activating process in the early stage of Fusobacterium nucleatum infected PDLSCs. International Journal of Oral Science, 15 (1): (1-14). [PMID:36754953] [10.1038/s41368-022-00213-0]
19. Huanyu Wang, Qianru Lin, Mingwang Liu, Wen Ding, Nanhai Weng, Hao Ni, Jing Lu, Mingsheng Lyu, Shujun Wang. (2023) Molecular Docking and Site-Directed Mutagenesis of GH49 Family Dextranase for the Preparation of High-Degree Polymerization Isomaltooligosaccharide. Biomolecules, 13 (2): (300). [PMID:36830669] [10.3390/biom13020300]
20. Zhen-Ping Zou, Yue Du, Ting-Ting Fang, Ying Zhou, Bang-Ce Ye. (2022) Biomarker-responsive engineered probiotic diagnoses, records, and ameliorates inflammatory bowel disease in mice. Cell Host & Microbe, [PMID:36758520] [10.1016/j.chom.2022.12.004]
21. Baizhu Chen, Xiaoge Zhang, Lili Cheng, Xiaomei Chen, Junjie Tang, Peng Zhang, Chen Wang, Jie Liu. (2022) Surface programmed bacteria as photo-controlled NO generator for tumor immunological and gas therapy. JOURNAL OF CONTROLLED RELEASE, [PMID:36528194] [10.1016/j.jconrel.2022.12.030]
22. Congyang Mao, Wanyu Jin, Yiming Xiang, Yizhou Zhu, Jun Wu, Xiangmei Liu, Shuilin Wu, Yufeng Zheng, Kenneth M. C. Cheung, Kelvin Wai Kwok Yeung. (2022) Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon–Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects. ADVANCED MATERIALS, 35 (9): (2208681). [PMID:36524686] [10.1002/adma.202208681]
23. Dan Zhao, Mengyu Xu, Kang Dai, Huan Liu, Yan Jiao, Xincai Xiao. (2022) The preparation of chiral carbon dots and the study on their antibacterial abilities. MATERIALS CHEMISTRY AND PHYSICS, [10.1016/j.matchemphys.2022.127144]
24. Jian Yao, Lun Gui, Qizhang Long. (2022) A chlorogenic acid esterase from a metagenomic library with unique substrate specificity and its application in caffeic and ferulic acid production from agricultural byproducts. BIOCATALYSIS AND BIOTRANSFORMATION, [10.1080/10242422.2021.1969370]
25. Jiaheng Wang, Nuo Zhang, Yawen Huang, Shunyi Li, Guimin Zhang. (2022) Simple and efficient enzymatic procedure for p-coumaric acid synthesis: Complete bioconversion and biocatalyst recycling under alkaline condition. BIOCHEMICAL ENGINEERING JOURNAL, [10.1016/j.bej.2022.108693]
26. Xiaofu Wang, Yunsong Zhao, Sufang Zhang, Xinping Lin, Huipeng Liang, Yingxi Chen, Chaofan Ji. (2022) Heterologous Expression of the Lactobacillus sakei Multiple Copper Oxidase to Degrade Histamine and Tyramine at Different Environmental Conditions. Foods, 11 (20): (3306). [PMID:37431050] [10.3390/foods11203306]
27. Tiancheng Tang, Benwei Zhu, Zhong Yao. (2022) Biochemical characterization and elucidation the action mode of a new PL25 family ulvan lyase from marine bacterium Alteromonas sp. TK-45 (2). Algal Research-Biomass Biofuels and Bioproducts, [10.1016/j.algal.2022.102854]
28. Xiaoxiang Hu, Huaxiang Deng, Yajun Bai, Tai-Ping Fan, Xiaohui Zheng, Yujie Cai. (2022) Heterologous expression and characterization of a borneol dehydrogenase from Arabidopsis lyrate and its application for the enzymatic resolution of rac-camphor. Molecular Catalysis, [10.1016/j.mcat.2022.112572]
29. Jiang Zhu, Jing Feng, Kai Tian, Chong Li, Mei Li, Xinghui QIU. (2022) Functional characterization of CYP6G4 from the house fly in propoxur metabolism and resistance. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, [PMID:36127048] [10.1016/j.pestbp.2022.105186]
30. Linhan Li, Tao Zhang, Gengrong Zhang, Guoqing Zhou, Fei Yang, Erlong Wang, Tianqiang Liu, Gaoxue Wang. (2022) High immune efficiency of bacterial nanocellulose loaded MSRV G protein vaccine for bath immunization. AQUACULTURE, [10.1016/j.aquaculture.2022.738579]
31. Xue-Yong Li, Jia-Ying Huang, Qiang Zhou, Yuan-Yuan Xu, Ponnandy Prabhu, Ye-Wang Zhang. (2022) Immobilization of Alcohol Dehydrogenase, Acetaldehyde Lyase, and NADH Oxidase for Cascade Enzymatic Conversion of Ethanol to Acetoin. Energies, 15 (12): (4242). [10.3390/en15124242]
32. Xinyu Wang, Jicong Zhang, Ke Li, Bolin An, Yanyi Wang, Chao Zhong. (2022) Photocatalyst-mineralized biofilms as living bio-abiotic interfaces for single enzyme to whole-cell photocatalytic applications. Science Advances, 8 (18): [PMID:35522739] [10.1126/sciadv.abm7665]
33. Jiacheng Shi, Rui Shu, Xiuyuan Shi, Yunfei Li, Jiangge Li, Yi Deng, Weizhong Yang. (2022) Multi-activity cobalt ferrite/MXene nanoenzymes for drug-free phototherapy in bacterial infection treatment. RSC Advances, 12 (18): (11090-11099). [PMID:35425054] [10.1039/D2RA01133F]
34. Wang Jing, Chen Qiaoyu, Wang Xin, Chen Kequan, Ouyang Pingkai. (2022) The Biosynthesis of D-1,2,4-Butanetriol From d-Arabinose With an Engineered Escherichia coli. Frontiers in Bioengineering and Biotechnology, [PMID:35402410] [10.3389/fbioe.2022.844517]
35. Hong-Ru Zhao, Bing-Mei Su, Yi-Bing Shi, Juan Lin. (2022) Construction of efficient enzyme systems for preparing chiral ethyl 3-hydroxy-3-phenylpropionate. ENZYME AND MICROBIAL TECHNOLOGY, [PMID:35364555] [10.1016/j.enzmictec.2022.110033]
36. Cong Chen, Fei Yu, Xinli Wen, Shuna Chen, Kaixi Wang, Feiquan Wang, Jianming Zhang, Yuanyuan Wu, Puming He, Youying Tu, Bo Li. (2022) Characterization of a new (Z)-3:(E)-2-hexenal isomerase from tea (Camellia sinensis) involved in the conversion of (Z)-3-hexenal to (E)-2-hexenal. FOOD CHEMISTRY, [PMID:35183969] [10.1016/j.foodchem.2022.132463]
37. Ling Zheng, Zilong Guo, Yinxiao Xu, Benwei Zhu, Zhong Yao. (2022) Biochemical characterization and immobilization of a novel pectate lyase ErPL2 for efficient preparation of pectin oligosaccharides. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:35151709] [10.1016/j.ijbiomac.2022.02.022]
38. Lichao Chen, Bo Wang, Haohao Ren, Yanan Wu, Defu Lyu, Yanan Ouyang, Qiyi Zhang, Yonggang Yan. (2022) Arg−Gly−Asp peptide functionalized poly-amino acid/ poly (p-benzamide) copolymer with enhanced mechanical properties and osteogenicity. Biomaterials Advances, [PMID:35527153] [10.1016/j.msec.2021.112627]
39. Zhou Li-Jian, Guo Li-Bin, Wei Wei, Lv Zhi-Xiang, Zhang Ye-Wang. (2021) A Novel Chondroitin AC Lyase With Broad Substrate Specificity From Pedobacter rhizosphaerae: Cloning, Expression, and Characterization. Frontiers in Bioengineering and Biotechnology, [PMID:35004658] [10.3389/fbioe.2021.808872]
40. Li Xue-Yong, Xu Meng-Qiu, Liu Hui, Zhou Qiang, Gao Jian, Zhang Ye-Wang. (2021) Preparation of combined cross-linked enzyme aggregates containing galactitol dehydrogenase and NADH oxidase for l-tagatose synthesis via in situ cofactor regeneration. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 45 (2): (353-364). [PMID:34797400] [10.1007/s00449-021-02665-w]
41. Guotao Mao, Fangyuan Wang, Jie Wang, Pengyu Chen, Xiaodong Zhang, Hongsen Zhang, Zhimin Wang, Andong Song. (2021) A sustainable approach for degradation and detoxification of malachite green by an engineered polyphenol oxidase at high temperature. Journal of Cleaner Production, [10.1016/j.jclepro.2021.129437]
42. Jiawei Guo, Fengwei Li, Fangyuan Cheng, Li Ma, Xiaohui Liu, Pradeepraj Durairaj, Gang Zhang, Dandan Tang, Xiangtian Long, Wei Zhang, Lei Du, Xingwang Zhang, Shengying Li. (2021) Bacterial Biosynthetic P450 Enzyme PikCD50N: A Potential Biocatalyst for the Preparation of Human Drug Metabolites. JOURNAL OF ORGANIC CHEMISTRY, [PMID:34662127] [10.1021/acs.joc.1c01407]
43. Qingman Li, Qihua Jiang, Pengcheng Gu, Lianju Ma, Yiwu Wang. (2021) Engineering of a Novel, Magnetic, Bi-Functional, Enzymatic Nanobiocatalyst for the Highly Efficient Synthesis of Enantiopure (R)-3-quinuclidinol. Catalysts, 11 (9): (1126). [10.3390/catal11091126]
44. Yongzhong Jiang, Menglu Hu, An-An Liu, Yi Lin, Linlin Liu, Bo Yu, Xiaoming Zhou, Dai-Wen Pang. (2021) Detection of SARS-CoV-2 by CRISPR/Cas12a-Enhanced Colorimetry. ACS Sensors, [PMID:33683104] [10.1021/acssensors.0c02365]
45. Shifeng Xiao, Yafei Lu, Qiuping Wu, Jiaying Yang, Jierui Chen, Suyue Zhong, David Eliezer, Qiulong Tan, Chengchen Wu. (2021) Fisetin inhibits tau aggregation by interacting with the protein and preventing the formation of β-strands. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:33662414] [10.1016/j.ijbiomac.2021.02.210]
46. Li-Bin Guo, Chen-Yuan Zhu, Yi-Bei Wu, Xiao-Man Fan, Ye-Wang Zhang. (2021) A novel chondroitin AC lyase from Pedobacter xixiisoli: Cloning, expression, characterization and the application in the preparation of oligosaccharides. ENZYME AND MICROBIAL TECHNOLOGY, [PMID:33812567] [10.1016/j.enzmictec.2021.109765]
47. Yunxiu Liu, Yu Tian, Qiuyang Han, Jie Yin, Junchuan Zhang, Yue Yu, Weizhong Yang, Yi Deng. (2020) Synergism of 2D/1D MXene/cobalt nanowire heterojunctions for boosted photo-activated antibacterial application. CHEMICAL ENGINEERING JOURNAL, [10.1016/j.cej.2020.128209]
48. Ning Hou, Qiaoruo Wang, Yang Sun, Xianyue Li, Qiuying Song, Xinxin Jiang, Baoxin Li, Xinyue Zhao, Hailian Zang, Dapeng Li, Chunyan Li. (2020) A novel biodemulsifier of Bacillus mojavensis XH1 – Oxalate decarboxylase with the potential for demulsification of oilfield emulsion. JOURNAL OF HAZARDOUS MATERIALS, [PMID:33321372] [10.1016/j.jhazmat.2020.124737]
49. Guo Xiaolei, Wu Weibin, Zhang Mingliang, Wu Licheng, Huang Jianzhong. (2020) A bi-enzymatic cascade to yield pyruvate as co-substrate for l-tyrosine production. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 104 (23): (10005-10018). [PMID:33128613] [10.1007/s00253-020-10975-4]
50. Ying Zhou, Rufang Deng, Xinlan Xu, Ziyin Yang. (2020) Enzyme Catalytic Efficiencies and Relative Gene Expression Levels of (R)-Linalool Synthase and (S)-Linalool Synthase Determine the Proportion of Linalool Enantiomers in Camellia sinensis var. sinensis. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:32829629] [10.1021/acs.jafc.0c04381]
51. Zelong Li, Runtao Zhu, Yongqi Liu, Jiaqi Li, Haofeng Gao, Nan Hu. (2020) γ-Glutamyltranspeptidase from Bacillus amyloliquefaciens: transpeptidation activity enhancement and L-theanine production. ENZYME AND MICROBIAL TECHNOLOGY, [PMID:32912696] [10.1016/j.enzmictec.2020.109644]
52. Xiaoxiang Hu, Yajun Bai, Tai-Ping Fan, Xiaohui Zheng, Yujie Cai. (2020) A novel type alanine dehydrogenase from Helicobacter aurati: Molecular characterization and application. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:32534087] [10.1016/j.ijbiomac.2020.06.067]
53. Shi-Yu Zhang, Ze-Wang Guo, Xiao-Ling Wu, Xiao-Yang Ou, Min-Hua Zong, Wen-Yong Lou. (2020) Recombinant expression and characterization of a novel cold-adapted type I pullulanase for efficient amylopectin hydrolysis. JOURNAL OF BIOTECHNOLOGY, [PMID:32198062] [10.1016/j.jbiotec.2020.03.007]
54. Jun Ma, Weifeng Lin, Liangbo Xu, Sihang Liu, Weili Xue, Shengfu Chen. (2020) Resistance to Long-Term Bacterial Biofilm Formation Based on Hydrolysis-Induced Zwitterion Material with Biodegradable and Self-Healing Properties. LANGMUIR, [PMID:32154728] [10.1021/acs.langmuir.0c00006]
55. Guoyan Zhang, Hossain M. Zabed, Junhua Yun, Jiao Yuan, Yufei Zhang, Yang Wang, Xianghui Qi. (2020) Two-stage biosynthesis of D-tagatose from milk whey powder by an engineered Escherichia coli strain expressing L-arabinose isomerase from Lactobacillus plantarum. BIORESOURCE TECHNOLOGY, [PMID:32105844] [10.1016/j.biortech.2020.123010]
56. Cai-Yun Liu, Wen-Bin Su, Li-Bin Guo, Ye-Wang Zhang. (2020) Cloning, expression, and characterization of a novel heparinase I from Bacteroides eggerthii. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, [PMID:31900079] [10.1080/10826068.2019.1709977]
57. Bing-Mei Su, Ze-Hui Shao, Ai-Peng Li, Muhammad Naeem, Juan Lin, Li-Dan Ye, Hong-Wei Yu. (2019) Rational Design of Dehydrogenase/Reductases Based on Comparative Structural Analysis of Prereaction-State and Free-State Simulations for Efficient Asymmetric Reduction of Bulky Aryl Ketones. ACS Catalysis, [10.1021/acscatal.9b04778]
58. Jiang Zhu, Kai Tian, Christopher A. Reilly, Xinghui Qiu. (2019) Capsaicinoid metabolism by the generalist Helicoverpa armigera and specialist H. assulta: Species and tissue differences. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, [PMID:31973854] [10.1016/j.pestbp.2019.11.013]
59. Peng Ji, Haiqin Huang, Shirui Yuan, Le Wang, Siqi Wang, Yiwei Chen, Na Feng, Hanitrarimalala Veroniaina, Ziheng Wu, Zhenghong Wu, Xiaole Qi. (2019) ROS-Mediated Apoptosis and Anticancer Effect Achieved by Artesunate and Auxiliary Fe(II) Released from Ferriferous Oxide-Containing Recombinant Apoferritin. Advanced Healthcare Materials, 8 (23): (1900911). [PMID:31701665] [10.1002/adhm.201900911]
60. Qiang Zhang, Dan Xu, Qiuyan Guo, Wenjun Shan, Jun Yang, Tongtong Lin, Shefang Ye, Xi Zhou, Yunlong Ge, Shengli Bi, Lei Ren. (2019) Theranostic Quercetin Nanoparticle for Treatment of Hepatic Fibrosis. BIOCONJUGATE CHEMISTRY, [PMID:31644261] [10.1021/acs.bioconjchem.9b00631]
61. Kai Tian, Jiang Zhu, Mei Li, Xinghui Qiu. (2019) Capsaicin is efficiently transformed by multiple cytochrome P450s from Capsicum fruit-feeding Helicoverpa armigera. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, [PMID:31027574] [10.1016/j.pestbp.2019.02.015]
62. Jiang-Wei Shen, Jia-Mei Qi, Xiao-Jian Zhang, Zhi-Qiang Liu, Yu-Guo Zheng. (2018) Significantly increased catalytic activity of Candida antarctica lipase B for the resolution of cis-(±)-dimethyl 1-acetylpiperidine-2,3-dicarboxylate. Catalysis Science & Technology, 8 (18): (4718-4725). [10.1039/C8CY01340C]
63. Yujiao Long, Jinren Ni, Zuhui Wang. (2015) Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes. WATER RESEARCH, [PMID:26233659] [10.1016/j.watres.2015.07.035]
64. Liu Junjie, Wu Shengxi, Zhang Lu, Zhang Mingjun, Wu Haotian, Shi Miwan, Weng Yuan, Ye Wenqiao, Gu Kui. (2024) A novel electrochemical immunosensor for the ultrasensitive detection of vascular endothelial growth factor based on MB@Cu-MOFs/MWCNTs composite. MICROCHIMICA ACTA, 192 (1): (1-13). [PMID:39730872] [10.1007/s00604-024-06884-2]
65. Dezheng Peng, Yang Li, Linlin Si, Bo Zhu, Peng Wu, Yibang Li, Dongfang Tang, Yu Liu, Yunxiao Zhang. (2024) A two-step method preparation of semaglutide through solid-phase synthesis and inclusion body expression. PROTEIN EXPRESSION AND PURIFICATION, [PMID:38527576] [10.1016/j.pep.2024.106477]
66. Jingqi Sun, Jiaxin Li, Yanzhi Xing, Han Leng, Wei Chen, Yanfeng Zhang, Xuwei Chen. (2025) Accurately sensing analysis of active adenine DNA glycosylase (MutY) via the high identification/excision capability to specific base-mismatches of dsDNA chains. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:40054823] [10.1016/j.ijbiomac.2025.141789]
67. Yidan Chen, Tiantian Feng, Xiaohong Zhu, Yuting Tang, Yao Xiao, Xiuhua Zhang, Sheng-Fu Wang, Dong Wang, Wei Wen, Jichao Liang, Huayu Xiong. (2024) Ambient Synthesis of Porphyrin-Based Fe-Covalent Organic Frameworks for Efficient Infected Skin Wound Healing. BIOMACROMOLECULES, [PMID:38720431] [10.1021/acs.biomac.4c00261]
68. Ting Wu, Huashan Sun, Wenyao Wang, Bin Xie, Zhengjie Wang, Jianqi Lu, Anming Xu, Weiliang Dong, Jie Zhou, Min Jiang. (2024) Boosting extracellular FastPETase production in E. coli: A combined approach of cognate chaperones co-expression and vesicle nucleating peptide tag fusion. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39566783] [10.1016/j.ijbiomac.2024.137857]
69. Yicong Lu, Xiao Zheng, Wan-Qiu Liu, Shengjie Ling, Jian Li. (2024) Chitin-Functionalized Cell-Free System Enables Sustainable Biocatalysis and Gene Expression. ACS Sustainable Chemistry & Engineering, [10.1021/acssuschemeng.4c07695]
70. Li Yang-Nan, Zhu Chen-Yuan, Xu Chen-Lu, Yu Shen, Huan Tong, Zhang Ye-Wang. (2024) Cloning, expression, and characterization of a heparinase III coupled with heparinase I for enzymatic depolymerization of heparin. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 41 (1): (1-12). [PMID:39710799] [10.1007/s11274-024-04225-2]
71. Feifei Wang, Dianlong Jia, Qingcui Song, Xiaomeng Lu, Guozi Xia, Jun Li, Xiaodong Jia, Mingliang Gu, Fengjiao Yuan, Zhengping Wang, Zhangyong Xia. (2025) Co-delivery of TRAIL and bortezomib via liposomes demonstrates enhanced antitumor efficacy in vivo. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, [10.1016/j.jddst.2025.106602]
72. Jingjing Dong, Yanli Wang, Xiaoxiao Li, Ying Chen, Rui Fan, Nana Wang, Zhengyu Jin, Yuxiang Bai. (2024) Comparison of a novel GtfB-type 4,6-α-glucanotransferase from Fructilactobacillus sanfranciscensis Gs2 for converting starch in the gelatinized and granular systems. Food Bioscience, [10.1016/j.fbio.2024.104118]
73. Zhuangzhuang Huang, Junru Zhou, Jialing Wang, Sheng Xu, Cheng Cheng, Jiangfeng Ma, Zhen Gao. (2025) Complementary Distant and Active Site Mutations Simultaneously Enhance Catalytic Activity and Thermostability of α-Galactosidase. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:39899880] [10.1021/acs.jafc.4c12426]
74. Binbin Chen, Jiahui Huang, Yingchun Liu, Lirong Yang, Qi Wang, Haoran Yu. (2025) Computational Study on the Catalytic Mechanism of UstD Catalyzing the Synthesis of γ-Hydroxy-α-Amino Acids. Journal of Chemical Information and Modeling, [PMID:39913657] [10.1021/acs.jcim.4c01998]
75. Qing Wang, Jiajia You, Yichen Li, Jie Zhang, Yi Wang, Meijuan Xu, Zhiming Rao. (2024) Continuous Evolution of Protein through T7 RNA Polymerase-Guided Base Editing in Corynebacterium glutamicum. ACS Synthetic Biology, [PMID:39700484] [10.1021/acssynbio.4c00606]
76. Guocong Luo, Zhaolin Huang, Yingying Zhu, Jiajun Chen, Xiaodong Hou, Dawei Ni, Wei Xu, Wenli Zhang, Yijian Rao, Wanmeng Mu. (2024) Crystal structure and structure-guided tunnel engineering in a bacterial β-1,4-galactosyltransferase. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39265897] [10.1016/j.ijbiomac.2024.135374]
77. Lin Ge, Yu Xia, Wenxin Xu, Ruobing Jia, Tingting Zhang. (2025) Efficient Biosynthesis of Gastrodin by UDP-Glycosyltransferase from Rauvolfia serpentina. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, [PMID:40147927] [10.4014/jmb.2501.01002]
78. Ziqi Liang, Qiao Zhou, Yicheng Li, Xiaoqing Liu, Yiwen Shen, Jian Tian, Xiaolu Wang, Xing Qin, Yuan Wang, Huiying Luo, Xiaojun Yang, Bin Yao, Tao Tu. (2025) Efficient C25-Hydroxylation of Vitamin D3 Utilizing an Artificial Self-Sufficient Whole-Cell Cytochrome P450 Biocatalyst. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:40112285] [10.1021/acs.jafc.4c12356]
79. Ai Yulin, Huang Yusong, Zhao Hongru, Su Bingmei, Lin Juan. (2025) Engineered phenylalanine hydroxylase coupled with an effective cofactor synthesis and regeneration system for high-yield production of 5-hydroxytryptophan. Bioresources and Bioprocessing, 12 (1): (1-14). [PMID:40047997] [10.1186/s40643-025-00846-z]
80. Jingyu Zhao, Huanhuan Sun, Gege Wang, Qi Wang, Yipeng Wang, Qingbin Li, Shuangyu Bi, Qingsheng Qi, Qian Wang. (2024) Engineering Chimeric Chemoreceptors and Two-Component Systems for Orthogonal and Leakless Biosensing of Extracellular γ-Aminobutyric Acid. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:38860925] [10.1021/acs.jafc.4c00041]
81. Runtao Zhu,Jiao Zhang,Lin Wang,Yunfeng Zhang,Yang Zhao,Ying Han,Jing Sun,Xi Zhang,Ying Dou,Huaxiong Yao,Wei Yan,Xiaozhou Luo,Junbiao Dai,Zhuojun Dai. (2024) Engineering functional materials through bacteria-assisted living grafting. Cell Systems, [PMID:38460522] [10.1016/j.cels.2024.02.003]
82. Yuan Li, Shu-Fang Li, Lin Zhang, Ya-Ping Xue, Yu-Guo Zheng. (2024) Engineering of a hydroxysteroid dehydrogenase with simultaneous enhancement in activity and thermostability for efficient biosynthesis of ursodeoxycholic acid. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 90 (9): [PMID:39207158] [10.1128/aem.01237-24]
83. Weijun Leng, Ying Li, Xin Liang, Li Yuan, Xiuting Li, Ruichang Gao. (2024) Engineering of protein glutaminase for highly efficient modification of fish myofibrillar protein through structure-based and computational-aided strategy. FOOD CHEMISTRY, [PMID:39154467] [10.1016/j.foodchem.2024.140845]
84. Miao Tian, Yuan-Yuan Xu, Yang-Nan Li, Shen Yu, Yi-Lin Wang, Xiao-Lai Ma, Ye-Wang Zhang. (2024) Engineering of Substrate-Binding Domain to Improve Catalytic Activity of Chondroitin B Lyase with Semi-Rational Design. CURRENT ISSUES IN MOLECULAR BIOLOGY, 46 (9): (9916-9927). [PMID:39329943] [10.3390/cimb46090591]
85. Shen Yu, Xue-Yong Li, Wen-Jie Si, Ye-Wang Zhang. (2024) Enhanced Stabilization Effect of Cofactor Thiamine Pyrophosphate in the Covalent Immobilization of Formolase. Catalysts, 14 (12): (911). [10.3390/catal14120911]
86. Feng Xinming, Cui Xinyu, Wang Kun, Liu Juanjuan, Meng Dongdong. (2024) Enzymatic characterization of a thermostable 6-phosphogluconate dehydrogenase from Hydrogenobacter thermophilus and its application for NADH regeneration. 3 Biotech, 15 (1): (1-8). [PMID:39650808] [10.1007/s13205-024-04165-6]
87. Muhammad Waheed Iqbal, Xinrui Tang, Tahreem Riaz, Shahid Mahmood, Yufei Zhang, Mei Zhao, Junhua Yun, Jia Li, Xianghui Qi. (2024) Exploiting the biocatalytic potential of co-expressed l-fucose isomerase and d-tagatose 3-epimerase for the biosynthesis of 6-deoxy-l-sorbose. BIOORGANIC CHEMISTRY, [PMID:38350272] [10.1016/j.bioorg.2024.107189]
88. Xiaotong Xu, Xiaoshuang Yan, Xuerong Jin, Juyi Li, Qiaoxia Hu, Dong-Hyun Ahn, Chao Zhong. (2025) Expression and characterization of colanic acid-degrading enzyme from Escherichia phage phi92 and analysis of its hydrolysate composition and structure. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39909247] [10.1016/j.ijbiomac.2025.140646]
89. Tong Zhang, Shubin Li, Lei Chen, Weiwen Zhang, Tao Sun. (2024) Extended Toolboxes Enable Efficient Biosynthesis of Multiple Products from CO2 in Fast-Growing Synechococcus sp. PCC 11901. ACS Sustainable Chemistry & Engineering, [10.1021/acssuschemeng.4c04497]
90. Tengfei Xiong, Mingyue Yu, Jiang Zhu, Kai Tian, Mei Li, Xinghui Qiu. (2024) Functional characterization of Helicoverpa assulta CYP6B6 in insecticide metabolism. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, [PMID:38685236] [10.1016/j.pestbp.2024.105857]
91. Chang Liu, Qi Yan, Xuying Ding, Meijun Zhao, Chen Chen, Qian Zheng, Huiying Yang, Yining Xie. (2024) Functional modification of recombinant brain-derived neurotrophic factor and its protective effect against neurotoxicity. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:38621565] [10.1016/j.ijbiomac.2024.131610]
92. Xiaohong Zhu, Tiantian Feng, Yidan Chen, Yao Xiao, Wei Wen, Xiuhua Zhang, Dong Wang, Shengfu Wang, Jichao Liang, Huayu Xiong. (2024) GSH-depleted Cu-covalent organic frameworks for multimodal synergistic therapy against diabetic infections. CHEMICAL ENGINEERING JOURNAL, [10.1016/j.cej.2024.150770]
93. Meng-Fan Feng, Li-Miao Qin, Bo Sun, Xianghui Yu, Yuqing Wu. (2024) Harnessing HPV Virus-like Nanoparticles for Efficient Circular RNA Delivery. ACS Applied Nano Materials, [10.1021/acsanm.4c03770]
94. Chen-Lu Xu, Chen-Yuan Zhu, Yang-Nan Li, Jian Gao, Ye-Wang Zhang. (2024) Heparinase III with High Activity and Stability: Heterologous Expression, Biochemical Characterization, and Application in Depolymerization of Heparin. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:38307881] [10.1021/acs.jafc.3c07197]
95. Jingjing Zhang, Jing Huang, Yufan Yang, Daxin Zhao, Cong Wang, Peng Zhong, Jianhua Jia, Weifan Dang, Qiwei Lu, Chengyu Zhang, Xiaohui Yan. (2024) Icaritin production from Epimedium folium extract by a one-pot enzymatic cascade of a multifunctional glycosidase and rhamnosidase. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39557269] [10.1016/j.ijbiomac.2024.137784]
96. Chenyu Song, Wei Xu, Cuie Guang, Ting Xue, Wanmeng Mu. (2024) Identification and application of a novel patulin degrading enzyme from Cyberlindnera fabianii. FOOD RESEARCH INTERNATIONAL, [PMID:39147475] [10.1016/j.foodres.2024.114846]
97. Long Zhang, Jinna Cui, Wei Liu, Zhanying Liu. (2024) Impact of membrane gene overexpression on endoglucanase secretion in ruminal cellulolytic Escherichia coli. PROCESS BIOCHEMISTRY, [10.1016/j.procbio.2024.11.006]
98. Chengyu Lu, Ziwei Chen, Yuvaraj Ravikumar, Guoyan Zhang, Xinrui Tang, Yufei Zhang, Mei Zhao, Wenjing Sun, Xianghui Qi. (2024) Improving Catalytic Efficiency of L-Arabinose Isomerase from Lactobacillus plantarum CY6 towards D-Galactose by Molecular Modification. Foods, 13 (11): (1727). [PMID:38890956] [10.3390/foods13111727]
99. Xinyi Wang, Wenhong Li, Xiang Yang, Mingwei Yang, Yucheng Gu, Zhao Du, Jingyi Yang, Mingxia Wen, Yoonseong Park, Chunyang Huang, Yueping He. (2024) Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos. PEST MANAGEMENT SCIENCE, [PMID:39166741] [10.1002/ps.8382]
100. Kai Tian, Jiang Zhu, Xinghui Qiu. (2024) Metabolism of Furanocoumarins by Three Recombinant CYP9A Proteins From the Polyphagous Cotton Bollworm Helicoverpa armigera. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 117 (3): (e70004). [PMID:39494619] [10.1002/arch.70004]
101. Bingbing Xia, Zhiwei Li, Jinao Wei, Ganpeng Hu, Jingwen Yang, Hongbin Zhang, Xueqin Hu. (2024) Modification of the Loop Region Near the Substrate Tunnel to Alter the Hydrolytic Process of Dextranase. JOURNAL OF BIOTECHNOLOGY, [PMID:38185430] [10.1016/j.jbiotec.2024.01.003]
102. Wen-Kai Liu, Xiu-Xin Ren, Lian Xu, Juan Lin. (2025) Modular cascade with engineered HpaB for efficient synthesis of hydroxytyrosol. BIOORGANIC CHEMISTRY, [PMID:39764921] [10.1016/j.bioorg.2025.108125]
103. Wen-Kai Liu, Bing-Mei Su, Xin-Qi Xu, Lian Xu, Juan Lin. (2024) Multienzymatic Cascade for Synthesis of Hydroxytyrosol via Two-Stage Biocatalysis. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:38940657] [10.1021/acs.jafc.4c04228]
104. Zifu Ni, Linshang Zhang, Azhen Nie, Huan Wang, Xiaoling Wu. (2024) One-Pot Synthesis of β-Alanine from Fumaric Acid via an Efficient Dual-Enzyme Cascade Biotransformation. Biomolecules, 14 (12): (1553). [PMID:39766260] [10.3390/biom14121553]
105. Lizhu Aer, Qifa Jiang, Linling Zhong, Qiuyue Si, Xianghong Liu, Yan Pan, Juan Feng, Hongjuan Zeng, Lixia Tang. (2024) Optimization of polyethylene terephthalate biodegradation using a self-assembled multi-enzyme cascade strategy. JOURNAL OF HAZARDOUS MATERIALS, [PMID:38901251] [10.1016/j.jhazmat.2024.134887]
106. Qi Houbao, Gao Haiting, Li Meihui, Sun Tianyong, Gu Xiufeng, Wei Li, Zhi Mengfan, Li Zixuan, Fu Dachuan, Liu Yiran, Wei Ziyi, Dou Yu, Feng Qiang. (2025) Parvimonas micra promotes oral squamous cell carcinoma metastasis through TmpC-CKAP4 axis. Nature Communications, 16 (1): (1-19). [PMID:40055343] [10.1038/s41467-025-57530-1]
107. Yuqiu Wu, Fanjin Meng, Jiaolong Fu, Yuyang Zhou, Huan Fan, Xin Ju, Liangzhi Li. (2024) PDA/SiO2 microspheres immobilize dual enzymes to increase temperature tolerance and catalyze the production of D-ribulose and D-xylulose from D-xylose. Molecular Catalysis, [10.1016/j.mcat.2024.113900]
108. Yuanyan Wei, Shuting Geng, Qihang Chen, Jiayue Chen, Zhijun Fan, Yi Li, Yu Si, Yuerong Yang, Ziwei Liang, Jianhai Jiang. (2024) Protocol for sorting cancer stem cells using a combination of anti-CD133 antibody and lectin cyanovirin-N. STAR Protocols, [PMID:39565690] [10.1016/j.xpro.2024.103443]
109. Lixin Kang, Haoran Li, Kexin Lin, Shen Hu, Song Liu, Yuben Qiao, Ying Wang, Aitao Li. (2025) Regioselectivity Switching in CYP107Pdh-Catalyzed VD3 Hydroxylation: A Structure-Guided Approach To Improve Calcidiol Production. ACS Catalysis, [10.1021/acscatal.5c00444]
110. Liang Pengda, Lv Bei, Chen Ke, Li Dawei. (2024) Sensitive aptasensing of ATP based on a PAM site-regulated CRISPR/Cas12a activation. MICROCHIMICA ACTA, 191 (7): (1-11). [PMID:38867016] [10.1007/s00604-024-06477-z]
111. Wang Xiaolu, Wang Qianqian, Wang Hong, Cai Guodi, An Yana, Liu Peiqing, Zhou Huihao, Chen Hong-Wu, Ji Shufeng, Ye Jiantao, Wang Junjian. (2025) Small protein ERSP encoded by LINC02870 promotes triple negative breast cancer progression via IRE1α/XBP1s activation. CELL DEATH AND DIFFERENTIATION, [PMID:39799200] [10.1038/s41418-025-01443-5]
112. Yuwen Wei, Fulong Li, Yukun Zheng, Youxiang Liang, Yan Du, Huimin Yu. (2025) Strengthening core-region hydrogen-bond networks and rigidifying surface loop to enhance thermostability of an (R)-selective transaminase converting chiral hydroxyl amines. JOURNAL OF BIOTECHNOLOGY, [PMID:40058651] [10.1016/j.jbiotec.2025.03.006]
113. Jialing Wang, Jie Chen, Kemin Lv, Zhen Gao, Jiahuang Li, Bin Wu, Bingfang He, Gerhard Schenk. (2024) Structure-Guided Engineering Unveils Deeper Substrate Channel in Processive Endoglucanase EG5C-1 Contributing to Enhanced Catalytic Efficiency and Processivity. ACS Synthetic Biology, [PMID:39579149] [10.1021/acssynbio.4c00562]
114. Bingjie Xu, Dan Yu, Chenlu Xu, Yujie Gao, Hengqiu Sun, Lei Liu, Yang Yang, Dongming Qi, Jindan Wu. (2024) Study on synergistic mechanism of molybdenum disulfide/sodium carboxymethyl cellulose composite nanofiber mats for photothermal/photodynamic antibacterial treatment. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:38521322] [10.1016/j.ijbiomac.2024.130838]
115. Sirui Zhang, Jingdan Wang, Rong Yu, Haiping Liu, Shuyan Liu, Kai Luo, Jin'e Lei, Bei Han, Yanjiong Chen, Shaoshan Han, E Yang, Meng Xun, Lei Han. (2024) The role of universal stress protein Usp1413 in meropenem adaptive resistance and environmental stress responses in Acinetobacter baumannii. Current Research in Microbial Sciences, [PMID:39758053] [10.1016/j.crmicr.2024.100332]
116. Di Junhua, Qin Lizhen, He Yu-Cai. (2022) Valorization of biomass to furfuryl alcohol via chemoenzymatic cascade catalysis by deep eutectic solvent-silica heterogeneous catalyst and reductase biocatalyst in the aqueous system. Biomass Conversion and Biorefinery, 13 (17): (15837-15844). [10.1007/s13399-021-02171-9]
117. Jie Cui, Yuxin Xia, Leilei Jin, Jishuang Chen, Zongli Huo, Fei Shen, Li Mi. (2025) A highly sensitive and anti-interference Bio-QDs biosensor for electrochemical detection calcium ion in plant tissue culture medium. MICROCHEMICAL JOURNAL, [10.1016/j.microc.2025.113284]
118. Wang Qing, Zhang Jie, Zhao Zhe, Li Yichen, You Jiajia, Wang Yi, Li Xiangfei, Xu Meijuan, Rao Zhiming. (2024) Dual genetic level modification engineering accelerate genome evolution of Corynebacterium glutamicum. NUCLEIC ACIDS RESEARCH, [PMID:38967005] [10.1093/nar/gkae577]
119. Wei He, Benshun Chen, Bin Yin, Jianren Ye, Yucai He. (2024) Efficient Biosynthesis of Monacolin J through Enzymatic Hydrolysis Using a Recombinant Lovastatin Hydrolase. Processes, 12 (9): (2006). [10.3390/pr12092006]
120. Dingtao Tang, Jiajie Quan, Zhengjiao Gao, Bingfeng He, Yu Hou, Peipei Fan, Meidong Pan, Jiali Yang. (2025) Prenylation of Flavanones by an Aromatic Prenyltransferase from Fusarium globosum. MOLECULES, 30 (7): (1558). [PMID:40286157] [10.3390/molecules30071558]
121. Kang Wei, Wu Zhiyue, Huang Xinzhi, Qi Hongbin, Wu Jiaxuan, Wang Jiahui, Li Jing, Wu Sijin, Kang Byung-Ho, Li Bo, Ma Juncai, Xue Chuang. (2025) Time-dependent catalytic activity in aging condensates. Nature Communications, 16 (1): (1-16). [PMID:40730570] [10.1038/s41467-025-62074-5]
122. Qiong Wang, Mei Zhao, Hossain M. Zabed, Xinrui Tang, Jiaojiao Chen, Jiayin Wang, Haoyang Li, Xianghui Qi. (2025) Data-Driven Mining of a Thermostable Lipase with Molecular Insights into Mechanisms of Its Substrate Specificity. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:40590090] [10.1021/acs.jafc.5c02801]
123. Chen Xue, Zhou Xing-Yue, Lan Cai, Fu Hai-Jun, Li Zhi-Chao, Chen Meng-Yi, Wen Yong-Ping, Yu Lu, Qin Da-Lian, Wu An-Guo, Wu Jian-Ming, Zhou Xiao-Gang. (2025) Araloside A Induces Raf/MEK/ERK-Dependent Autophagy to Mitigate Alzheimer’s and Parkinson’s Pathology in Cellular and C. elegans Models. MOLECULAR NEUROBIOLOGY, [PMID:40742404] [10.1007/s12035-025-05242-4]
124. Yuvaraj Ravikumar, Abdullah Arsalan, Xinrui Tang, Guoyan Zhang, Xianghui Qi. (2025) Enhanced Thermostability of an Improved l-Arabinose Isomerase Mutant for d-Tagatose Synthesis by Molecular Dynamics Simulations-Guided Rational Redesign of Flexible Regions. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:40802916] [10.1021/acs.jafc.5c03396]
125. Meichen Zhu, Qinzhe Lin, Mengning Zhong, Jian Dong, Leyi Zhao, Qiyun Wu, Huilin Ren, Wenting Du, Jun Li. (2025) Rational engineering of an imine reductase unlocks stereochemical gatekeeping for efficient synthesis of Evocalcet chiral intermediate. Molecular Catalysis, [10.1016/j.mcat.2025.115415]
126. Jiang Cheng, Li Luli, Li Jialu. (2025) Characterization of a β-N-acetylhexosaminidase CgNagase20 from Chaetomium globosum with antifungal activity against Fusarium sporotrichioides. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 41 (8): (1-13). [PMID:40788452] [10.1007/s11274-025-04524-2]
127. Hanchi Chen, Shijie Lu, Liying Zha, Yazhou Lu, Qixin Wang, Linjiang Zhu, Xiaolong Chen. (2025) Molecular dynamics guided mining and engineering of glucosyltransferase in synthesizing menthyl-β-glucoside. AICHE JOURNAL, [10.1002/aic.70032]
128. Yingying Zhu, Dawei Ni, Zeyu Li, Zhebin Hao, Liang Wang, Wanmeng Mu. (2025) NADPH regeneration for efficient biosynthesis of indigo by flavin-containing monooxygenase and formate dehydrogenase. ENZYME AND MICROBIAL TECHNOLOGY, [PMID:40773977] [10.1016/j.enzmictec.2025.110731]
129. Ziyu Kuai, Yibing Huang, Fengjie Hao, Wantong Jiang, Mengmeng Niu, Yu qin, Ao Song, Yuran Deng, Youzhong Wan, Quan Luo. (2025) Atomic-Level Engineering of Protein-Templated Gold Clusterzymes with Tunable Fluorescence and Multi-Enzyme Activities for Antiaging Treatment. ACS Applied Materials & Interfaces, [PMID:40957724] [10.1021/acsami.5c11714]
130. Wenwei Tang, Meihuan Tong, Hui Liao, Peiqi Zhang, Xinping Zeng. (2025) Construction of engineering bacteria for manganese oxidation and degradation of aureomycin by biogenic manganese oxides. Journal of Environmental Chemical Engineering, [10.1016/j.jece.2025.119402]
131. Qiang Wang, Hossain M. Zabed, Mei Zhao, Xianghui Qi. (2025) A Deeper Insight into the Thermostability of a Novel Laccase Designed by Data-Driven Mining and Rational Engineering. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:40906826] [10.1021/acs.jafc.5c03876]
132. Yuke Qin, Shuting Hu, Yibin Yang, Xiaoqiang Jia. (2025) Whole-cell biosensors with regulatory circuits based on a synthetic dual-input promoter enabling the highly sensitive detection of ultra-trace Cd2+. Synthetic and Systems Biotechnology, [PMID:41079772] [10.1016/j.synbio.2025.09.008]
133. Tengfei Xiong, Mingyue Yu, Mei Li, Christopher A. Reilly, Xinghui Qiu. (2024) CYP6B6 mediated adaptation to capsaicinoids in the generalist Helicoverpa armigera and specialist H. assulta: Transcriptional response and metabolic detoxification. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39631573] [10.1016/j.ijbiomac.2024.138286]
134. Yizhen Zhang, Yu-Cai He, Cuiluan Ma. (2024) Efficient synthesis of vanillylamine through bioamination of lignin-derived vanillin by recombinant E. coli containing ω-transaminase from Caulobacter sp. D5 in dimethyl sulfoxide-water. BIORESOURCE TECHNOLOGY, [PMID:39321936] [10.1016/j.biortech.2024.131526]
135. Fan Jiyuan, Sun Hao, Fang Jiawen, Gao Yafan, Ding Haojie, Zheng Bin, Kong Qingming, Zhuo Xunhui, Lu Shaohong. (2024) Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species. Parasites & Vectors, 17 (1): (1-13). [PMID:38389080] [10.1186/s13071-024-06180-1]
136. Mengna Chen, Yanping Li, Qinghua He, Junjie Zeng, Xiaojiang Li, Zhui Tu. (2025) Homogeneous bioluminescent immunosensor for aflatoxin B1 detection via unnatural amino acid-based site-specific labeling. FOOD CONTROL, [10.1016/j.foodcont.2025.111358]
137. Tianyi Zhang, Yingying Zhao, Cong Zhu, Xi Zhu, Xiaohua Zhu, Ye Qiu, Zhou Nie, Chunyang Lei. (2025) CRISPR/Cas12a Protein Switch Powered Label-Free Electrochemical Biosensor for Sensitive Viral Protease Detection. ANALYTICAL CHEMISTRY, [PMID:40165508] [10.1021/acs.analchem.5c00547]
138. Ketao Yan, Yidan Chen, Tiantian Feng, Ziwei Xiang, Yuzhu Long, Yuzhu Yuan, Wei Wen, Xiuhua Zhang, Shengfu Wang, Suxiao Wang, Jichao Liang, Huayu Xiong. (2025) Multifunctional Nanomedicine with Microenvironment-Regulated Controlled Release for Promoting Healing of Infected Diabetic Wounds. ACS Applied Nano Materials, [10.1021/acsanm.5c00921]
139. Zepeng Li, Runmin Wang, Xiantai Lai, Wenyi Liao, Runfeng Liao, Zhuohong Wu, Guoyan Zhang, Xianghui Qi. (2025) Enabling Stable Recycling of L-Arabinose Isomerase Through Whole-Cell Immobilization for Efficient and Cost-Effective D-Tagatose Production. Foods, 14 (9): (1538). [PMID:40361621] [10.3390/foods14091538]
140. Puhong Yi, Yue Xu, Yuhua Hao, Zhiqiang Liu, Yaping Xue, Liqun Jin, Yuguo Zheng. (2025) Structural Analysis and Mutability Landscape-Guided Engineering of ω-Transaminase for Improved Catalytic Performance. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:40244570] [10.1021/acs.jafc.4c13142]
141. Yan Wang, Suyuan Su, Chaofeng Wang, Congyang Mao, Xiangmei Liu, Hanpeng Liu, Zhaoyang Li, Shengli Zhu, Hui Jiang, Zhenduo Cui, Yufeng Zheng, Shuilin Wu. (2025) Hydrogel-Mediated Delivery of Baicalein for the Effective Therapy of MRSA-Infected Diabetic Wounds by Immune Response and Moderate Photothermal Effect. Biomaterials Science, [PMID:40356501] [10.1039/D5BM00335K]
142. Dong Rui, Li Meihui, Gu Xiu Feng, Gao Haiting, Wei Ziyi, Qi Houbao, Zhang Jun, Feng Qiang. (2025) The surface protein Gbp of Fusobacterium nucleatum inhibits osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2. Journal of Translational Medicine, 23 (1): (1-18). [PMID:40369630] [10.1186/s12967-025-06569-1]
143. Xuan Wang, Xiaolin Tian, Jiawei Guo, Fangyuan Cheng, Mingyu Liu, Shanmin Zheng, Yangliu Feng, Ying Lv, Yuanning Li, Shengying Li, Xingwang Zhang. (2025) Carboxylic Acid Tailoring in Thioquinolobactin Biosynthesis. JOURNAL OF NATURAL PRODUCTS, [PMID:40498497] [10.1021/acs.jnatprod.5c00331]
144. Yuhan Zhao, Xiaojie Zhou, Mei Li, Xinghui Qiu. (2025) Functional characterization of CYP9J26, a cytochrome P450 associated with insecticide resistance in the arbovirus vector Aedes albopictus. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:40533012] [10.1016/j.ijbiomac.2025.145280]
145. Naying Zhang, Zefeng Ren, Dongsheng Wei, Meng Yang, Meng Niu, Chunxiao Shen, Xuanyan Jin, Maolin Wei, Jaehwan Choi, Myeongsam Park, Zhengqun Li. (2025) Discovery, expression, and characterization of highly tolerant superoxide dismutases from extremophiles for potential industrial applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:40541892] [10.1016/j.ijbiomac.2025.145272]
146. Wang Li, Song YingJie, Guo Yi, Chen Guobing, Zhang Hua, Liang Changhai. (2025) Modification of Nitrile Hydratase by Substrate Channel Engineering Regulates Monocyanamide Synthesis. CATALYSIS LETTERS, 155 (7): (1-12). [10.1007/s10562-025-05096-4]
147. Pan Zhou, YanLi Yu, WeiYu Qi, XiaoJuan Wang, YouLi Yu, JianDong Wang, Li Zhang, ZhengQing Yu, TingLi Liu. (2025) PLGA nanoparticles as an efficient carrier in Toxoplasma GAP45: a more effective vaccine against acute toxoplasmosis than traditional ones. Frontiers in Immunology, [PMID:40625742] [10.3389/fimmu.2025.1600399]
148. Penghui Zhu, Bochen Pan, Chen Li, Benwei Zhu, Zhong Yao, Yun Sun. (2025) Biochemical characterization and immobilization of a PL25 family ulvan lyase for efficient degradation of Enteromorpha polysaccharide. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:40571024] [10.1016/j.ijbiomac.2025.145518]
149. Yue Liu, Wansen Tan, Jingjun Hong. (2025) Co-Expression and Purification of the Complex of Single-Chain H2B-H2A.Z and Histone Chaperone YL1. Current Protocols, 5 (5): (e70151). [PMID:40421937] [10.1002/cpz1.70151]
150. Yiming Zhao, Jingjun Hong. (2025) Purification of Recombinant Histones and Mononucleosome Assembly. Current Protocols, 5 (5): (e70155). [PMID:40402568] [10.1002/cpz1.70155]
151. Yi Zheng, Yingbo Yuan, Yuanfei Han, Tianyuan Su, Qingsheng Qi. (2025) Recyclable chemical-enzymatic hydrolysis cascade for the degradation and closed-loop recycling of blended fabrics. BIORESOURCE TECHNOLOGY, [PMID:40581055] [10.1016/j.biortech.2025.132897]
152. Jiawei Guo, Qiaoyu Zhang, Yang Shen, Fangyuan Cheng, Moli Sang, Xuan Wang, Yunjun Pan, Mingyu Liu, Hao-Bing Yu, Bo Hu, Sheng Wang, Liangzhen Zheng, Ce Geng, Chaofan Yang, Lianzhong Luo, Gang Zhang, Lei Du, Yuanning Li, Wei Zhang, Yandong Zhang, Binju Wang, Shengying Li, Xingwang Zhang. (2025) Deciphering the Thiolactonization Mechanism in Thiolactomycin Biosynthesis. Journal of the American Chemical Society, [PMID:40537968] [10.1021/jacs.4c14296]
153. Xiao-Ting Zhou, Ke-Yan Wei, Shan Li, Qi Shen, Ya-Ping Xue, Yu-Guo Zheng. (2025) A Triple-Focus Workflow of a Novel Nitrilase for Simultaneously Optimizing Activity, Thermostability, and Specificity. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:41039745] [10.1021/acs.jafc.5c07983]
154. Alexey Tarabarov, Xingxing Wang, Yige Lu, Jing Jiang, Yifei Cheng, Kexin Huang, Jiayao Yang, Zhuqian Xiao, Fangfang Fan, Dapeng Li, Hongpeng Wang, Jun Huang. (2025) Elucidating protein M performance and its binding modes in antibody purification via integrated site-specific immobilization and molecular dynamics. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:41093200] [10.1016/j.ijbiomac.2025.148306]
155. Ranran Ma, Fred Mwabulili, Weiwei Peng, Weibin Ma, Yuhui Yang, Yanli Xie. (2025) Dual Substrate Catalytic Detoxification of Aflatoxin B1 and Zearalenone by a Novel Enzyme PsCopA from Pseudomonas for Food and Feed Safety. TOXICON, [PMID:41151655] [10.1016/j.toxicon.2025.108629]
156. Xianglong Li, Haoxian Huang, Yachuan Yuan, Mingdong Wang, Xuanyu Cao, Yanbin Feng, Song Xue. (2025) Enhancing CO2 fixation catalyzed by salicylic acid decarboxylase with remodeled tunnels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:41176010] [10.1016/j.ijbiomac.2025.148584]
157. Yiming Zhao, Jingjun Hong. (2025) Expression, Purification, and ATP Hydrolysis Activity Measurement of CtISWI and CtATPase. Current Protocols, 5 (10): (e70241). [PMID:41133289] [10.1002/cpz1.70241]
158. Mengjie Lin, Wenxin Chen, Cunshan Zhang, Jiewei He, Lina Wu, Ailin Liu, Xinhua Lin, Liqing Lin. (2025) Thiamine-regulated fluorescence based on the oxidase-mimicking activity of BSA-MnO2 nanosheets for the detection of β-galactosidase and Escherichia coli. MICROCHEMICAL JOURNAL, [10.1016/j.microc.2025.116246]
159. Man Zhao, Mengying Yu, Huiru Yuan, Yiting Shen, Zhiqiang Liu, Yuguo Zheng. (2025) Linker Peptide Engineering Combined With SA-PVA Immobilization in Fed-Batch Biocatalysis for High-Efficiency Carcinine Synthesis. BIOTECHNOLOGY AND BIOENGINEERING, [PMID:41235884] [10.1002/bit.70101]
160. Ruyue Dong, Jiaying Wang, Jian Tian, Guoshun Xu, Ziqi Liang, Xing Qin, Xiaolu Wang, Xiaoqing Liu, Huiying Luo, Bin Yao, Yaru Wang, Tao Tu. (2025) ESTEEM Strategy: Revolutionizing Enzymatic Catalysis through the Evolutionary Gateway of Enzyme Tunnels. ACS Catalysis, [10.1021/acscatal.5c03170]
161. Xue-Chun Li, Hong-Jie Xie, Zheng-Xiong Zhou, Bin Sun, Jun-Jie Lin, Xiao-Dan Li, Ren Wang. (2025) An Exporter of Leucine, LeuE Functions as an Exporter of 1-Aminocyclopropane-1-Carboxylic Acid in Escherichia coli. BIOCHEMICAL ENGINEERING JOURNAL, [10.1016/j.bej.2025.110038]
162. Jiashi Li, Zhong Lu, Xiaoli Zeng, Hanxiao Zhang, Haixia Lu, Lifang Feng, Junli Zhu. (2025) Efficient quenching activity and thermostability of N-acyl homoserine lactonase AiiA against fish spoilage mediated by Pseudomonas fluorescens via rational design. FOOD MICROBIOLOGY, [PMID:41478679] [10.1016/j.fm.2025.105002]
163. Ke Zhan, Yuting Zhao, Haiying Li, Shan Jiang. (2025) Octopod-shaped silver–gold alloy for NIR-driven synergistic photothermal and catalytic bacterial ablation. COLLOIDS AND SURFACES B-BIOINTERFACES, [PMID:41380240] [10.1016/j.colsurfb.2025.115353]
164. Yuhan Zhao, Xiaojie Zhou, Mei Li, Xinghui Qiu. (2025) Aedes albopictus CYP6Z8 metabolizes multiple AChE-inhibiting insecticides. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, [PMID:41629003] [10.1016/j.pestbp.2025.106934]
165. Jinwei Zhai, Wansen Tan, Lei Ji, Jingjun Hong. (2025) Fermentation and Purification of Recombinant Human Type III Collagen Expressed in Escherichia coli. Current Protocols, 5 (12): (e70284). [PMID:41452303] [10.1002/cpz1.70284]
166. Guimeng Xiao, Xiaonan Zhao, Shaoyun Shi, Jianxing Lu, Guiling Li, Fengying Gong, Xinming Feng, Juanjuan Liu, Dongdong Meng. (2025) Thermostable Whole-Cell Biocatalysts Enable Sustainable and Practical Synthesis of N-Acetylneuraminic Acid. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:41456346] [10.1021/acs.jafc.5c10911]
167. Bao Fan, Jiale Xiong, Cuiluan Ma, Yu-Cai He. (2025) Bioproduction of Gastrodin from Lignin-Based p-Hydroxybenzaldehyde Through the Biocatalysis by Coupling Glycosyltransferase UGTBL1-Δ60 and Carbonyl Reductase KPADH. Processes, 14 (1): (55). [10.3390/pr14010055]
168. Yang Xinyi, Ning Tingting, Zhang Wanru, Hou Songyuan, Su Jiayi, Li Wenkun, Wu Jing. (2025) Fusobacterium nucleatum drives gastric cancer metastasis via Gbp-CypA-NF-κB-mediated CXCL8 crosstalk between tumor cells and mast cells. Cell Communication and Signaling, 24 (1): (6). [PMID:41316398] [10.1186/s12964-025-02561-w]
169. Yun-Fei Zhu, Xing-Yue Zhou, Cai Lan, Yong-Ping Wen, Hai-Jun Fu, Zhi-Chao Li, Ya-Ping Li, Shi-Ying Li, Fei-Hong Huang, Long Wang, Lu Yu, Da-Lian Qin, An-Guo Wu, Jian-Ming Wu, Xiao-Gang Zhou. (2025) Tricin Delays Aging and Enhances Muscle Function via Activating AMPK-Mediated Autophagy in Diverse Model Organisms. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:40243450] [10.1021/acs.jafc.4c12619]
170. Jianfeng He, Yongyi Zhu, Zichao Tian, Mengqin Liu, Anmin Gao, Wangmi Fu, Fei Lu, Yutong Sun, Yajun Guo, Rongqing Pan, Yuchen Ji, Jianxiang Chen, Huasong Lu, Juan Lin, Xingguo Liang, Chun Kim, Chun Zhou, Huipeng Jiao. (2025) ZBP1 senses spliceosome stress through Z-RNA:DNA hybrid recognition. MOLECULAR CELL, [PMID:40267921] [10.1016/j.molcel.2025.04.004]
171. Qing Wang, Jing Sun, Lei Zang, Huaxiong Yao, Lin Wang, Runtao Zhu, Jie Li, Simin Zeng, Hongting Tang, Teng Wang, Ji Liu, Bo Wang, Bo Li, Zhiyuan Liu, Zhuojun Dai. (2025) Programmable cell aggregation by a synthetic biosilicification approach. iScience, [PMID:40546950] [10.1016/j.isci.2025.112519]
172. Zhi Li, Jing Wang, Juan Du, Jun Li, Yanan Song, Junji Zhu, Ziyi Li, Wei Zhou, Shiyun Chen, Lijie Yang, Maohui Feng, Xiaolian Cai, Katryn J. Stacey, Wuhan Xiao. (2025) Human OCEL1 senses bacterial infection to unlock inflammatory responses. Science Immunology, 10 (114): [PMID:41417924] [10.1126/sciimmunol.adk0853]
173. Qihan Jin, Yukai Wang, Dachuan Chen, Jinyang Liao, Zhanyuan Cui, Yuxuan Fan, Anping Zeng, Mingqi Xie, Longxing Cao. (2026) De novo design of small molecule–regulated protein oligomers. SCIENCE, 391 (6780): [PMID:41477902] [10.1126/science.ady6017]
174. Xiaoxiao Li, Ying Chen, Zhengyu Jin, Yuxiang Bai. (2026) Mining and Modifying Distal Amino Acid Residue Assisted with Tunnel Engineering to Regulate the Product Specificity of Limosilactobacillus fermentum NCC 2970 GtfB 4,3-α-Glucanotransferase. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:41494056] [10.1021/acs.jafc.5c14570]
175. Ye Wang, Heshi Tian, Qiannan Li, Zhenhua Ma, Yunna Jia, Xiaojun Liang, Xiuzhen Yu, Xiuyun Jiang, Yunhang Gao. (2026) Expression and application of α/β hydrolase from Kurthia gibsonii TYL-A1. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, [PMID:41538961] [10.1016/j.ecoenv.2026.119718]
176. Quanfu Wang, Xingshun Li, Yatong Wang, Yanhua Hou. (2026) Characteristics of cold-adapted arylesterase and low-temperature efficient PBAT degradation via cell surface display technology. JOURNAL OF HAZARDOUS MATERIALS, [PMID:41544593] [10.1016/j.jhazmat.2026.141150]
177. Yucheng Guo, Zhenlin Ouyang, Wenbo He, Qian Qin, Jiaxin Zhang, Ting Yu, Min Jiao, Peter M. Hwang, Fang Zheng, Serge Muyldermans, Yurong Wen. (2026) Mechanistic diversity of Bacteroides fragilis toxins and neutralization with single domain antibody. Cell Chemical Biology, 33 (1): (102-116). [PMID:41544614] [10.1016/j.chembiol.2025.12.009]
178. Qingao Xue, Ze Liang, Yi Zhang, Fei Wang, Fulian Wang, Lili Liu, Guang Yang, Lei Yan. (2026) Rapid discovery of repurposed drugs targeting SARS-CoV-2 spike HR1 by DNA-encoded library screening. BIOORGANIC CHEMISTRY, [PMID:41691755] [10.1016/j.bioorg.2026.109627]
179. Xin Li, Haoyu Chai, Zhifan Yang, Cuiluan Ma, Yu-Cai He. (2026) Efficient synthesis of syringyl alcohol through bioreduction of lignin-derived syringaldehyde by newly constructed recombinant Escherichia coli C165F-V231D. BIORESOURCE TECHNOLOGY, [PMID:41672312] [10.1016/j.biortech.2026.134193]
180. Chi Wang, Fahui Sun, Heyu Zhao, Nan Zhang, Jiali Guan, Yuxing Zhou, Wentong Shuai, Hui Zheng, Jun He. (2026) Structural Basis of Nucleosome Deubiquitination by the Bidentate Calypso/Asx Complex. iScience, [PMID:41782825] [10.1016/j.isci.2026.114958]
181. Ning Wang, Meiling Jin, Zixuan Zhu, Yuxin Wang, Xinyu Li, Jing Xu, Shijunyi Cheng, Yuanying Zhu, Ruiqi Wang, Tong Xu, Furong Yin, Xinyue Li, Yue Ke, Haitao Yue. (2026) Ecological distribution and functional characterization of polyethylene-degrading enzymes from diverse metagenomes. SCIENCE OF THE TOTAL ENVIRONMENT, [PMID:41671813] [10.1016/j.scitotenv.2026.181486]
182. Zixuan Li, Pingping Wang, Haiting Gao, Mengfan Zhi, Xiufeng Gu, Tianyong Sun, Yushang Wang, Song Shen, Xiaomei Ma, Xiaoli Ji, Xiumei Zhang, Dongxu Liu, Qiang Feng. (2026) Parvimonas micra exacerbates periodontitis by infiltrating host cells through TmpC and circumventing lysosomal elimination via AppA. EBioMedicine, [PMID:41740230] [10.1016/j.ebiom.2026.106187]
183. Qian Zhong, Zhen Li, Waibin Duan, Peng Lei, Xiaoqi Xu, Hong Xu, Sha Li, Yibin Qiu. (2026) Programming Low-Molecular-Weight Hyaluronic Acid Biosynthesis in Bacillus amyloliquefaciens Via an Autoinducible Molecular Switch. ACS Synthetic Biology, [PMID:41706816] [10.1021/acssynbio.5c00570]
184. Xin Li, Haoyu Chai, Zhifan Yang, Yucai He, Cuiluan Ma. (2026) Bioproduction of 2,5-Furandimethanol from High Titer of 5-Hydroxymethylfurfural by Robust Mutant Alcohol Dehydrogenase Biocatalyst in Ternary Deep Eutectic Solvent Betaine:Malic Acid:Glycerol. ACS Sustainable Chemistry & Engineering, [10.1021/acssuschemeng.5c13655]
185. Wenmei Li, Chenchen Guo, Kaixin Wu, Qunrui Xie, Jianye Yang, Aiwen Shen, Qin Liu, Yuhong Ren. (2026) Rolling circle amplification-based self-assembled CpG nanoparticles: Immune activation and prevention of Edwardsiella piscicida. FISH & SHELLFISH IMMUNOLOGY, [PMID:41794159] [10.1016/j.fsi.2026.111260]
186. Lei Mingzhu, Zhu Wenying, Wang Chao, Zhang Fan, Li Miaolin, Zhang Yi, Qu Jia, Luo Zhiguo, Li Jintao, Li Zeng-Xia, Yin Miao, Lei Qun-Ying. (2026) Targeting methionine-induced pyroptosis enhances anti-tumor immunity. Science China-Life Sciences, [10.1007/s11427-025-3149-4]
187. Kai Li, Dongsheng Sun, Dongyang Zhang, Yang Liu, Xinli Li. (2026) High-titer nicotinamide adenine dinucleotide production via artificially designed pseudo-de novo biosynthesis pathway. BIORESOURCE TECHNOLOGY, [PMID:41916456] [10.1016/j.biortech.2026.134529]
188. Hong-Ru Zhao, Wen-Tao Shen, Bingmei Su, Lian Xu, Juan Lin. (2026) Multi-strategy engineering of transaminase enables a one-pot synergistic biocatalytic cascade to a key florfenicol chiral intermediate. GREEN CHEMISTRY, [10.1039/D5GC06908D]
189. Jingjing Kuang, Yu An, Jianguo Zhu, Kehua Jiang, Fa Sun, Kun Chen. (2026) An AviTag/BirA Platform for IL7: Minimized Activity Loss over Random Biotinylation. ACS Omega, [10.1021/acsomega.5c12146]
190. Yuqing Wang, Mingdong Wang, Xianglong Li, Xinyu Che, Xuanyu Cao, Jiangang Ren, Yanbin Feng, Song Xue. (2026) Structure-Guided Ancestral Evolution of HmfF Yields a Robust Decarboxylase for Terephthalic Acid Degradation. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:41773466] [10.1021/acs.jafc.5c14497]
191. Ye Meng, Liu Xinyu, Peng Zhengxiong, Li Shuaichao, Jia Xiaoyu, Yang Xiyuchen, Jiang Shan, Wang Feng, Yang Xiaobing, Yu Juping, Liu Wei, Gao Xiangdong. (2026) Engineered Bacteroides thetaiotaomicron sense gut inflammation and deliver therapeutic molecules to alleviate colitis in mice. Nature Microbiology, [PMID:42225846] [10.1038/s41564-026-02378-4]
192. Liqiang Zhang, Xiao Wei, Ke Zhang, Fan Ding, Ziliang Guo, Bin Zou, Wangxiao He, Wenjia Liu. (2026) Functional matrix vesicle replacement partially restores chemo-mechanical coupling in the aged bone niche. Cell Reports Medicine, [PMID:42462727] [10.1016/j.xcrm.2026.102923]
193. Xiangyu Zhao, Tao Wu, Sanan Wu, Yujin Chen, Yu Zhang, Jing Chen, Kaixuan Diao, Zaoke He, Jiawei Yan, Tianzhu Lu, Chao Xu, Lu Liu, Gaofeng Fan, Dongliang Xu, Xinxiang Li, Xiaopeng Xiong, Jianjun Cheng, Fang Bai, Xue-Song Liu. (2026) Deep learning reveals FLAD1-mediated mitochondrial metabolic reprogramming in hypoxic tumors. Cell Reports, 45 (7): [PMID:42467529] [10.1016/j.celrep.2026.117711]
194. Sun Ze-Rui, Xia Yu-Xin, Li Yi-Ran, Liu Li, Voglmeir Josef. (2026) Thermostability Engineering of a Chitin-Utilizing GlcNAc Deacetylase from Acinetobacter baumannii via Distal Mutations. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 74 (28): (22149-22158). [PMID:42443049] [10.1021/acs.jafc.6c07357]
195. Yunxiao Zhang, Bo Zhu, Binhui Peng, Peng Wu, Guyu Zhong, Yongchen Wang, Zhaofeng Wang, Xinyu Feng, Dongfang Tang, Dezheng Peng, Yu Liu. (2026) A Sequential Dual GLP-1R/GIPR Agonist-To-Antagonist Molecule Achieves Superior Weight Loss in Obese Mice. DIABETES OBESITY & METABOLISM, [PMID:42410323] [10.1111/dom.70967]
196. Yiwen Shen, Ziqi Liang, Lanxue Li, Wei Zhang, Yaru Wang, Jian Tian, Bin Yao, Huoqing Huang, Huiying Luo, Tao Tu. (2026) Glucose-to-Starch Conversion by a Synthetic Enzymatic Cascade. Engineering, [10.1016/j.eng.2026.04.021]
197. Liang Zhu, Lejun Liao, Yan Huang, Zhou Nie, Chunyang Lei. (2026) Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection. ANALYTICAL CHEMISTRY, [10.1021/acs.analchem.6c02971]
198. Tang Tianyu, Yang Wuping, Lu Minghao, Peng Ding, Li Shiqi, Cui Sijia, Ye Sunyi, Liu Jiandong, Wang Yifan, Wu Cunjin, Teng Haoyang, Huang Haojie, Xu Xin, Xia Dan, Zhu Yi. (2026) AURKB-mediated phosphorylation of metabolic enzyme CD73 drives immune suppression and renal cell carcinoma progression. CELL DEATH AND DIFFERENTIATION, [10.1038/s41418-026-01845-z]
199. Chen Yuehua, Liu Peng, Sheng Binbin, Xing Jiaxin, Ouyang Jia, Zheng Zhaojuan. (2026) Expanding Aldopentose Catalysis of a Novel PQQ-Dependent Glucose Dehydrogenase without Compromising Native d-Glucose Activity. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 74 (33): (26532-26543). [PMID:42677662] [10.1021/acs.jafc.6c08393]