计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
产品介绍:
墨汁荚膜染色对于观察新型隐球菌有很好的作用,印度墨汁属于非常优质的墨汁,新型隐球菌菌体周围存在粘多糖荚膜物,用印度墨汁染色法能证明荚膜的存在。染色后新型隐球菌的荚膜取代了墨汁中的胶状碳粒,菌体被清楚无色透明的晕圈环所绕着。
使用说明:
1、样本离心,取沉淀涂于载玻片上,在样本涂片处滴加墨汁。
2、加盖盖玻片,并防止气泡产生。用手指轻轻压一下,使样本与墨汁混合变薄。
3、在低倍镜下寻找有荚膜的细菌,并转高倍镜或油镜下仔细辨认。
注意事项:
1、已开封印度墨汁应在6个月内用完,每次用后尽量密封好,以免挥发或变质。
2、印度墨汁存储时间较长后易产生沉淀,如果镜下发现颗粒粗糙者应过滤后使用或弃用。
3、样本的纯度和浓度应适宜,浓度过低时在镜下很难寻找到新型隐球菌。
4、样本和印度墨汁分布应均匀。
5、若觉得墨汁太浓,可稍作稀释后使用。
6、为了您的安全和健康,请穿实验服并戴一次性手套操作。
染色结果:
| 新型隐球菌 | 宽厚透亮的荚膜,细胞壁明显 |
| 背景 | 黑色 |
India ink capsule staining is an effective method for observing Cryptococcus neoformans. India ink is a high-quality reagent for this assay. Cryptococcus neoformans is surrounded by a mucopolysaccharide capsule, which can be clearly demonstrated via India ink staining. After staining, the capsule excludes colloidal carbon particles in the ink, and the fungal cells are surrounded by distinct, colorless and transparent halos.
Instructions for Use:
1. Centrifuge the sample, collect the pellet and spread it evenly on a glass slide, then add a drop of India ink onto the smear.
2. Place a coverslip carefully to avoid air bubbles. Gently press the coverslip with your finger to spread the sample and ink into a thin layer.
3. Screen for encapsulated microorganisms under a low-power objective, then observe and identify in detail under a high-power objective or oil immersion objective.
Precautions:
1. Use opened India ink within 6 months. Seal the container tightly after each use to prevent volatilization and deterioration.
2. Precipitate may form after long-term storage of India ink. If coarse particles are observed under the microscope, filter the ink before use or discard it.
3. Ensure appropriate purity and concentration of the sample. A too-low concentration will make Cryptococcus neoformans difficult to detect microscopically.
4. Distribute the sample and India ink uniformly on the slide.
5. Dilute the ink slightly if it is overly concentrated.
6. Wear a lab coat and disposable gloves during operation for personal safety and health protection.
Staining Results:
| Cryptococcus neoformans. | Surrounded by a thick, transparent capsule with clearly visible cell walls |
| Background | Black |
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | I774775 | |
| 分析证书 | I774775 | |
| 分析证书 | I774775 |
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