一般在组织切片过程中，当某些组织内含有骨质或钙化灶时，含钙的组织不能直接用石蜡包埋切片。因为钙和石蜡之间的密度不同，不容易切出完整的切片。对含钙组织最好固定之后，再进行脱钙或者二者同时进行。然后进行下游操作如脱水、透明、浸蜡、包埋、切片。用于脱钙的试剂很多，脱钙剂包括有酸、无机酸、乙二胺四乙酸 (EDTA) 以及电解法脱钙。EDTA 是一种相对较好的螯合脱钙剂，对组织结构影响最小，可以较好的保存组织的某些酶类，经 EDTA 脱钙后的组织可以进行免疫组化和原位杂交染色。但是该法脱钙速度太慢，一般脱钙需要数周至数月。
JYBL-Ⅰ 脱钙液主要由稀酸、甲醛、甲酸等组成。其优点是：
①作用迅速，24～36 h 即可。
②对组织的结构损害小。
③脱钙完全。该产品为快速脱钙液，推荐用于日常骨组织病理样本的脱钙，对组织损伤小，但不宜用化学方法确定脱钙终点。
保存条件：
室温保存，一年有效。
系列产品概览
| 货号 | 名称 | 主要成分 | 脱钙时间 | 组织损伤风险 | 核心优势 | 适用标本类型 |
| J1525466 | JYBL-Ⅰ脱钙液 | 稀酸、甲醛、甲酸 | 24～36 h | 相对较小 | 速度与损伤的基础平衡 | 常规骨组织标本 |
| J1529419 | JYBL-Ⅱ脱钙液 | 稀酸、甲醛、甲酸 | 约 24 h（比Ⅰ更快） | 有一定损伤（比Ⅰ略高） | 速度优于Ⅰ | 常规骨组织标本 |
| J1529418 | JYBL-Ⅲ脱钙液 | 甲醛、50%甲酸 | 室温：2～3 天 | 相对最小（甲酸最温和） | 甲酸对组织损伤小，形态保存更佳 | 特殊骨组织标本 |
| J1529417 | JYBL-Ⅳ脱钙液 | 甲醛、甲酸（不含强酸） | 37℃加热：2～3 h
室温：2～3 天 | 相对较小，加热需控温 | 速度最快，专为骨穿刺微量样本设计 | 骨组织穿刺样本 |
自备材料：
1、PBS
2、蒸馏水
3、加热装置或微波炉
操作步骤 (仅供参考)：
1、骨组织脱钙时，取材不易过厚。
2、组织固定后，用 PBS 清洗。
3、组织用蒸馏水清洗。
4、组织转移至的 JYBL-Ⅰ 脱钙液中，脱钙。如果想加快脱钙速度，可以置于 37℃进行脱钙。
5、用蒸馏水冲洗数次。
6、常规脱水、包埋。
注意事项：
1、厚度 5 mm 的骨组织块脱钙时间一般脱钙 24～36h 即可。
2、适当加温能加快脱钙的速度，一般不应超过 37～40℃，温度过高容易使骨组织造成松散解体，因此不可大于 60℃。
3、脱钙应彻底，防止脱钙不足或过度。脱钙程度应控制在不影响组织切片的同时尽量缩短脱钙时间，以免脱钙过长引起组织损害。
4、脱钙用具避免使用金属容器，尽量使用玻璃容器。
5、骨组织脱钙应先固定后脱钙或脱钙固定同时进行，不应先脱钙后固定，以便减少组织的损伤程度。
6、每隔一段时间检测一次脱钙程度，脱钙过度会增加组织的损伤程度，影响染色结果。
7、为了您的安全和健康，请穿实验服并戴一次性手套操作。
脱钙终点的测定 (物理法)：
采用针刺、手掐、钳夹等方法，当骨组织变软或针刺时没有阻力即可终止脱钙。物理检测法会对组织结构有一定的损害，尽量避免用力过大。
During routine tissue sectioning, tissues containing bone or calcified lesions cannot be directly embedded in paraffin for sectioning. Due to the density difference between calcium and paraffin, intact sections are difficult to obtain. For calcium-containing tissues, decalcification should be performed after tissue fixation, or fixation and decalcification can be carried out simultaneously, followed by subsequent procedures including dehydration, clearing, infiltration, embedding and sectioning.A variety of decalcifying reagents are available, including organic acids, inorganic acids, ethylenediaminetetraacetic acid (EDTA) and electrolytic decalcification. As a superior chelating decalcifier, EDTA causes minimal damage to tissue structure and well preserves endogenous enzymes within tissues. Tissues decalcified with EDTA are compatible with immunohistochemistry and in situ hybridization staining. However, EDTA decalcification is extremely time-consuming, typically requiring weeks to several months to complete.
JYBL-Ⅰ Decalcifying Solution is mainly composed of dilute acid, formaldehyde, formic acid and other components. Its advantages are as follows:
① Fast decalcification, which can be finished within 24 to 36 hours;
② Slight damage to tissue structure;
③ Thorough decalcification.This product is a rapid decalcifying reagent recommended for decalcification of routine pathological bone tissue samples with low tissue damage. Chemical methods are not suitable for determining the decalcification endpoint for this product.Storage Conditions
Storage Conditions:
Store at room temperature; valid for 1 year.
Product Series Overview
| Cat. No. | Product Name | Main Components | Decalcification Time | Tissue Damage Risk | Core Advantages | Applicable Specimen Types |
| J1525466 | JYBL-Ⅰ Decalcifying Solution | Dilute acid, formaldehyde, formic acid | 24–36 h | Relatively low | Balanced speed and tissue preservation | Routine bone tissue specimens |
| J1529419 | JYBL-Ⅱ Decalcifying Solution | Dilute acid, formaldehyde, formic acid | Approx. 24 h (faster than Ⅰ) | Moderate (slightly higher than Ⅰ) | Faster decalcification than Ⅰ | Routine bone tissue specimens |
| J1529418 | JYBL-Ⅲ Decalcifying Solution | Formaldehyde, 50% formic acid | Room temperature: 2–3 days | Relatively minimal (formic acid is the mildest) | Minimal tissue damage; superior morphology preservation | Special bone tissue specimens |
| J1529417 | JYBL-Ⅳ Decalcifying Solution | Formaldehyde, formic acid (acid-free formulation) | 37°C incubation: 2–3 h | Relatively low; temperature control required | Fastest decalcification; designed for bone marrow biopsy | Bone marrow biopsy specimens |
Required Self-prepared Materials:
1. PBS
2. Distilled water
3. Heating device or microwave oven
Operating Procedures (For Reference Only):
1. Avoid overly thick sampling when preparing bone tissue for decalcification.
2. Rinse fixed tissues with PBS.
3. Wash tissues with distilled water.
4. Immerse tissues in JYBL-Ⅰ Decalcifying Solution for decalcification. Incubate at 37 ℃ to accelerate the decalcification process if needed.
5. Rinse the tissues several times with distilled water.
6. Perform standard dehydration and embedding procedures.
Precautions:
1. Decalcification generally takes 24-36 hours for bone tissue blocks with a thickness of 5 mm.
2. Moderate heating accelerates decalcification; the temperature should not exceed 37-40 ℃. Excessively high temperature will lead to loose and decomposed bone tissue, and the temperature must never go above 60 ℃.
3.Ensure complete decalcification to avoid insufficient or over-decalcification. Minimize decalcification duration while guaranteeing qualified sectioning quality to prevent tissue damage caused by prolonged decalcification.
4.Do not use metal containers during decalcification; glass containers are highly recommended.
5.Bone tissue must be fixed prior to decalcification, or fixation and decalcification shall be conducted concurrently. Decalcification before fixation is forbidden to reduce tissue damage.
6.Check the decalcification progress regularly. Over-decalcification will aggravate tissue damage and interfere with staining outcomes.
7.Wear a lab coat and disposable gloves during operation for personal safety and health protection.
Determination of Decalcification Endpoint (Physical Method):
Use acupuncture, pinching or clamping to test tissues. Terminate decalcification when the bone tissue softens or no resistance is felt during needling. This physical detection method may damage tissue structure, so excessive force should be avoided.
ZJ26F0838439
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