Biological Background of CHIC2

CHIC2 (Cysteine-Rich Hydrophobic Domain 2), also known as BRX or RINF, is a protein encoded by the CHIC2 gene located on human chromosome 4q12. The protein is characterized by a conserved cysteine-rich hydrophobic (CHIC) domain, which is thought to facilitate membrane association and protein–protein interactions critical to intracellular signaling pathways.

CHIC2 has attracted significant scientific interest because the CHIC2 gene locus is recurrently deleted or rearranged in certain hematological malignancies. Notably, deletions at 4q12 involving CHIC2 are associated with the formation of the FIP1L1-PDGFRA fusion gene, a driver mutation found in a subset of myeloid neoplasms with eosinophilia. This genomic context positions CHIC2 as a relevant marker and potential functional participant in disease pathogenesis.

Beyond its role as a deletion marker, CHIC2 protein is implicated in membrane-associated signaling and has been studied for its interactions with components of growth factor receptor pathways. Understanding CHIC2 biology may provide insights into leukemogenesis and broader oncogenic mechanisms.

Protein Characteristics and Product Specifications

Recombinant Human CHIC2 Protein (rp374608) is produced using established recombinant expression systems to ensure consistent quality for experimental reproducibility. The CHIC2 protein contains the defining CHIC domain, a structural motif conserved across species, which is important for its subcellular localization and function.

Researchers should consult the official product datasheet for Aladdin Scientific SKU rp374608 for confirmed specifications including molecular weight, purity grade, expression host, tag information, formulation buffer, and recommended storage conditions. Key specification details such as endotoxin levels, activity data, and validated applications are provided on the datasheet and should be verified prior to experimental use.

As with all recombinant proteins, batch-to-batch consistency is maintained through quality control testing. Users are advised to record the lot number from the product vial for traceability in publication and regulatory submissions.

Recommended Handling and Storage Protocol

Proper handling of Recombinant Human CHIC2 Protein is essential to preserve its biological activity and structural integrity. The following general guidelines apply to most lyophilized or liquid recombinant proteins; always verify against the product-specific datasheet.

• Reconstitution: If supplied lyophilized, reconstitute the protein in the buffer recommended on the datasheet (typically sterile PBS or a low-salt buffer with a carrier protein such as BSA). Allow the vial to equilibrate to room temperature before opening. Gently pipette to dissolve; do not vortex, as vigorous mixing can cause protein aggregation and denaturation.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles, which can reduce protein activity and promote aggregation.
• Storage: Store long-term at –80 °C. For short-term use (up to 1 month), –20 °C may be acceptable. Avoid repeated freeze-thaw cycles. Adding a carrier protein (e.g., 0.1% BSA) to the working stock can improve stability during storage.
• Handling: Work on ice when pipetting protein solutions. Use low-protein-binding tubes and pipette tips to reduce adsorption losses.
• Quality check: Prior to critical experiments, verify protein integrity by SDS-PAGE and confirm activity using a functional assay appropriate to your experimental system.

Research Applications

Recombinant Human CHIC2 Protein is a versatile reagent suitable for a range of in vitro research applications in oncology, molecular biology, and biochemistry:

• Protein–Protein Interaction Studies: CHIC2 can be used as bait or prey in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance (SPR) studies to identify and characterize binding partners relevant to membrane signaling complexes.
• Antibody Validation and Immunoassay Development: Recombinant CHIC2 serves as a positive control antigen for validating anti-CHIC2 antibodies in Western blotting, ELISA, and immunofluorescence assays, ensuring antibody specificity before use in complex biological samples.
• Structural and Biophysical Studies: The recombinant protein can be used for circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), or crystallography studies aimed at elucidating CHIC2 domain structure and folding.
• Functional Assays in Oncology Research: In the context of FIP1L1-PDGFRA-associated myeloid neoplasms and eosinophilic disorders, recombinant CHIC2 provides a research tool for investigating the functional consequences of CHIC2 loss or overexpression on cellular signaling.
• Biomarker Research: Given the association of CHIC2 deletion with specific hematological malignancies, the recombinant protein supports the development of diagnostic or prognostic assays.

Ordering, Quality Assurance, and Support

Recombinant Human CHIC2 Protein is available from Aladdin Scientific under SKU rp374608. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative. Bulk quantities and custom formulations may be available upon request.

Quality Assurance: All Aladdin Scientific recombinant proteins undergo rigorous quality control prior to release, including purity assessment by SDS-PAGE, identity confirmation, and where applicable, functional activity testing. A certificate of analysis (CoA) is available for each lot and should be requested at the time of purchase for documentation purposes.

Technical Support: For questions regarding reconstitution, optimal assay conditions, or troubleshooting experimental results with rp374608, contact Aladdin Scientific technical support. Detailed protocol recommendations and application notes are available from the product page.

Storage and Shipping: Products are shipped under conditions designed to maintain protein integrity. Upon receipt, inspect the package and store the product immediately under recommended conditions as stated on the datasheet. Report any concerns about shipping damage or product appearance to Aladdin Scientific customer service promptly.