What Is Recombinant Human GRAF Protein?

GRAF (GTPase Regulator Associated with Focal adhesion kinase) is a member of the Rho family GTPase-activating proteins (GAPs). It contains a characteristic BAR domain, a PH domain, a RhoGAP domain, and a C-terminal SH3 domain. GRAF acts as a negative regulator of RhoA GTPase activity, thereby influencing actin cytoskeleton dynamics, cell morphology, and membrane tubulation.

Recombinant Human GRAF Protein (SKU: rp374624) is a recombinant form of this human protein produced for use in biochemical and cell biology research. It enables investigators to study GRAF's enzymatic activity, protein–protein interactions, and its roles in signaling pathways without reliance on endogenous protein sources.

Biological Function and Research Significance

GRAF plays a critical role in regulating the Rho GTPase signaling axis. By stimulating the intrinsic GTPase activity of RhoA, GRAF promotes conversion of active RhoA-GTP to inactive RhoA-GDP, thus attenuating downstream effectors involved in stress fiber formation and actomyosin contractility.

Beyond cytoskeletal regulation, GRAF has been associated with endocytic membrane trafficking, myoblast differentiation, and has been reported as a candidate tumor suppressor gene in certain hematologic malignancies. These diverse roles make Recombinant Human GRAF Protein a valuable tool for researchers investigating cytoskeletal biology, cancer biology, and membrane dynamics.

Storage, Handling, and Quality Considerations

Proper storage and handling are essential to maintain the activity and integrity of Recombinant Human GRAF Protein. General best practices for recombinant proteins apply:

• Storage temperature: Store at –20 °C or –80 °C as indicated in the product datasheet. Avoid repeated freeze–thaw cycles.
• Reconstitution: If supplied in lyophilized form, reconstitute according to the datasheet instructions using the recommended buffer. Allow the vial to equilibrate to room temperature before opening.
• Aliquoting: Upon reconstitution, prepare single-use aliquots to minimize freeze–thaw degradation.
• Carrier protein: Some preparations include BSA as a stabilizer; confirm formulation details in the product-specific datasheet.

Always consult the product datasheet (absea#PP-8402) for lot-specific purity, concentration, and activity data.

Typical Applications of Recombinant Human GRAF Protein

Recombinant Human GRAF Protein is suitable for a range of in vitro research applications, including but not limited to:

• GAP activity assays: Measuring the ability of GRAF to stimulate GTP hydrolysis by RhoA or related GTPases using colorimetric or radioactive phosphate-release assays.
• Protein interaction studies: Investigating binding partners such as FAK (focal adhesion kinase) via co-immunoprecipitation, pull-down assays, or surface plasmon resonance (SPR).
• Structural and biophysical studies: Using the purified protein for X-ray crystallography, cryo-EM, or NMR studies of GRAF domains.
• Antibody validation: Serving as a positive control antigen for ELISA or Western blot validation of anti-GRAF antibodies.
• Cell-free reconstitution: Reconstituting cytoskeletal regulation pathways in cell-free systems.

Ordering and Support Information

Recombinant Human GRAF Protein is available from Aladdin Scientific under SKU rp374624. The corresponding supplier reference is absea#PP-8402.

For ordering, pricing, or availability inquiries, please contact Aladdin Scientific's customer service team or visit the product page on the Aladdin website. For technical questions regarding experimental design, reconstitution, or application optimization, our scientific support team is available to assist.

When placing an order, please have the SKU (rp374624) and your institution's purchasing information ready to expedite processing.