What Is Recombinant Human GEF-H1 Protein?

GEF-H1, encoded by the ARHGEF2 gene, is a member of the Dbl family of Rho guanine nucleotide exchange factors (GEFs). It catalyzes the exchange of GDP for GTP on RhoA, thereby activating downstream cytoskeletal and transcriptional pathways. A defining feature of GEF-H1 is its association with polymerized microtubules: when sequestered on microtubules the protein is inactive, and microtubule depolymerization releases and activates it, directly coupling microtubule dynamics to RhoA-dependent actin reorganization.

Recombinant Human GEF-H1 Protein (SKU rp374278) available from Aladdin Scientific is produced for use in biochemical and cell-biology research applications, providing a well-defined source of this exchange factor outside the complexity of cell lysates.

Biological Role and Research Significance

GEF-H1 sits at the intersection of microtubule dynamics and Rho GTPase signaling, making it relevant to a broad range of cell-biological processes:

• Cytoskeletal regulation: By activating RhoA, GEF-H1 promotes stress-fiber formation and actomyosin contractility.
• Cell migration and invasion: Modulation of GEF-H1 activity influences lamellipodia formation, directionality, and invasive behavior of cells.
• Mitosis: GEF-H1 has been implicated in regulating contractile-ring formation during cytokinesis.
• Epithelial barrier function: GEF-H1 contributes to tight-junction regulation and paracellular permeability.
• Oncology relevance: Dysregulation of GEF-H1 / ARHGEF2 has been associated with tumor invasion and metastasis in multiple cancer types.

These roles make Recombinant Human GEF-H1 Protein a valuable tool for in vitro GEF activity assays, protein–protein interaction studies, and structural investigations.

Product Specifications and Quality

Aladdin Scientific supplies Recombinant Human GEF-H1 Protein (SKU rp374278) as a research-grade reagent. Researchers should consult the current product datasheet for the most accurate and up-to-date specifications, including:

• Expression system: Confirm from the datasheet (commonly E. coli, baculovirus/insect cell, or mammalian cell systems are used for Rho-GEF family proteins).
• Tag information: His-tag or GST-tag details as specified on the datasheet.
• Purity: Typically assessed by SDS-PAGE; confirm target purity grade from the datasheet.
• Formulation and buffer: Refer to the current certificate of analysis (CoA) for buffer composition and any carrier protein.
• Storage and stability: Generally stored at −80 °C; avoid repeated freeze–thaw cycles. Refer to the datasheet for shelf life.

Note: Specific numerical values for purity percentage, concentration, and endotoxin level are not reproduced here to avoid inaccuracy; always verify against the current CoA and datasheet available on the Aladdin Scientific product page.

Recommended Applications

Recombinant Human GEF-H1 Protein is suitable for a variety of research applications, subject to validation in the user's specific experimental system:

• In vitro GEF activity assays: Measure nucleotide exchange activity toward RhoA using fluorescence-based (e.g., BODIPY-GDP) or radioactive assays.
• Binding and interaction studies: Pull-down assays, co-immunoprecipitation validation, surface plasmon resonance (SPR), and isothermal titration calorimetry (ITC) to characterize interactions with RhoA, microtubule components, or regulatory partners.
• Structural biology: Crystallography or cryo-EM studies of the DH-PH domain architecture.
• Inhibitor screening: Use as a target in small-molecule screening campaigns aimed at blocking GEF-H1–RhoA interaction.
• Antibody validation: Serve as a positive control antigen for Western blot or ELISA validation of anti-GEF-H1 antibodies.

Users are encouraged to optimize experimental conditions for their specific assay formats.

Ordering, Storage, and Handling Guidelines

Ordering: Recombinant Human GEF-H1 Protein is available from Aladdin Scientific under SKU rp374278. Contact your regional Aladdin Scientific representative or place an order via the Aladdin Scientific website. For bulk or custom quantities, request a quote through the customer-service portal.

Shipping: Recombinant proteins are typically shipped on dry ice. Inspect the package upon receipt and confirm the product arrived frozen. Any concerns regarding shipment condition should be reported to Aladdin Scientific customer service promptly.

Storage: Store at −80 °C upon receipt. Aliquot into single-use volumes before freezing to minimize freeze–thaw cycles, which can compromise protein activity and integrity.

Handling: Thaw on ice immediately before use. Avoid prolonged incubation at room temperature. Centrifuge briefly after thawing to collect any condensate. For long-term stability, refer to the product datasheet.