This experimental method was obtained from the official website of the Fourth Military Medical University

Operation method

Bacterial slide agglutination test

Principle

When the particulate antigen binds specifically to the corresponding antibody, it can gradually aggregate in the presence of an appropriate amount of electrolyte, resulting in the phenomenon of agglutination visible to the naked eye.

Materials and Instruments

Typhoid bacillus Dysentery bacillus
Saline
Slides Capillary Pipettes

Move

1. Take a clean slide and mark it with a crayon into three compartments with numbers. Under aseptic operation, add 1~2 drops of 1:10 diluted S. typhi diagnostic serum to compartments 1 and 2 with an inoculating loop, and add 1~2 drops of saline to the third compartment.

2. Under sterile operation, use the inoculation ring to take a little culture of S. typhi, mix it in the third compartment, and then mix it in the first compartment (you can't mix the first compartment first and then mix the third compartment, because it will make the diagnostic serum mix with saline and affect the control results), and then mix the bacteria with the saline or serum to make a homogeneous emulsion. At this time, the amount of bacteria should not be too much, so that the suspension is mildly turbid.

3. Take a small amount of dysentery bacillus culture in the same way, and mix it in the second compartment.

4. Gently shake the slide, after 1~2min visual observation, the appearance of milky white agglutination, that is, a positive reaction; still equal to the emulsion, that is, a negative reaction. If the result is not clear enough, put the slide under a low-power microscope.

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