AIE脂滴蓝色探针是一种基于AIE原理开发的三苯胺类脂滴探针，具有典型的AIE特性。产品具有细胞跨膜能力，只需简单地和细胞孵育，即可通过被动转运完成跨膜运输。
产品特性
AIE脂滴蓝色探针具有良好的聚集诱导发光特性，可以通过靶向脂滴的极性环境，特异性标记多种活细胞及固定细胞的脂滴结构，在与脂滴结合前后由于聚集状态的变化，其荧光强度会产生极为明显的变化，而未与脂滴结合的荧光探针基本不会发射出荧光信号。
图 1. HeLa细胞上的激光共聚焦成像效果图
与常见染料不同， AIE脂滴蓝色探针的斯托克斯位移大，可以明显与其他染料进行区分，减少成像中串色的可能性。并且，AIE脂滴蓝色探针操作简便，无需洗涤便可成像。同时AIE脂滴蓝色探针的生物相容性好，成像浓度高，可在多次扫描的状态仍然保证稳定的荧光信号输出，非常适合多次成像。
产品性质
化学式（Formula）
| C₃₃H₃₀N₂O
| 分子量（Molecular Weight）
| 470.62g/mol
| 纯度（Purity）
| ≥98% (HPLC)
| 工作浓度（Working Concentration）
| 100-200nM
| 半峰宽（Full width at half maximum）
| 450nm–550nm
| 最大吸收/发射波长（nm）
| λabs =412nm / Em= 497nm |
|
产品优势
1、细胞毒性相对较小，可以进行活细胞成像及固定细胞成像；
2、强抗光漂白能力，40 次共15 分钟的激光扫描，发射荧光强度不变；
3、背景信号低，无需洗涤即可快速成像。
实验方法
1、染料储备液准备：短暂离心，吹打均匀后，将探针溶液适当分装后避光保存于-20°C或更低温度；
2、染料工作液准备：取1μL AIE脂滴蓝色探针储备液加入到1-10mL 细胞培养液或适当的缓冲液中 (例如PBS)，得到终浓度100-1000nM的AIE脂滴蓝色探针工作液；
3、细胞染色：对于活细胞，用适量的工作染液孵育贴壁细胞30 min（最好置于细胞培养箱中）；对于固定细胞，待细胞固定后，用适量工作染液孵育固定细胞30 min，PBS清洗三次；染完后用共聚焦荧光显微镜或荧光显微镜观察，激发波长设为405 nm；收集418-500 nm的信号。
注意事项
1、使用前请先短暂离心，可根据细胞的不同适当调整染色浓度及时间；
2、于-20°C保存，避免反复冻融；
3、为了您的安全和健康，请穿实验服并戴一次性手套操作；
4、本品仅适用于科研用途。
AIE Lipid droplets Blue Probe is a triphenylamine-based lipid droplet probe developed based on the AIE principle, with typical AIE properties. The product has cell transmembrane ability; it can complete transmembrane transport through passive transport just by simply incubating with cells.
Product Characteristics
AIE Lipid droplets Blue Probe has excellent aggregation-induced emission properties. It can specifically label lipid droplet structures in various living cells and fixed cells by targeting the polar environment of lipid droplets. Due to the change in aggregation state before and after binding to lipid droplets, its fluorescence intensity will produce extremely obvious changes, while the fluorescent probes that do not bind to lipid droplets basically do not emit fluorescent signals.
Figure 1. Laser confocal imaging effect diagram of HeLa cells
Unlike common dyes, the AIE Lipid Droplet Blue Probe has a large Stokes shift, which can be clearly distinguished from other dyes and reduce the possibility of crosstalk in imaging. Moreover, the AIE Lipid Droplet Blue Probe is easy to operate and can be imaged without washing. At the same time, the AIE Lipid Droplet Blue Probe has good biocompatibility and high imaging concentration, and can still ensure stable fluorescent signal output under multiple scanning conditions, making it very suitable for multiple imaging.
Product Properties
| Product Properties | Details |
|---|
| Formula | C₃₅H₃₀N₂O | | Molecular Weight | 470.62 g/mol | | Purity | ≥98% (HPLC) | | Working Concentration | 100 - 200 nM | | Full Width at Half Maximum | 450 nm - 550 nm | | Max Absorption/Emission Wavelength (nm) | λabs = 412 nm / Em = 497 nm |
|
Product Advantages
- Relatively low cytotoxicity, applicable for both live cell imaging and fixed cell imaging;
- Strong anti-photobleaching ability, with the emitted fluorescence intensity remaining unchanged after 40 laser scans totaling 15 minutes;
Low background signal, enabling rapid imaging without the need for washing.
Experimental Methods
- Preparation of dye stock solution: Centrifuge briefly, pipette to mix evenly, then aliquot the probe solution appropriately and store in the dark at -20°C or lower;
- Preparation of dye working solution: Take 1μL of AIE Lipid Droplet Blue Probe stock solution and add it to 1-10mL of cell culture medium or an appropriate buffer (such as PBS) to obtain a working solution of AIE Lipid Droplet Blue Probe with a final concentration of 100-1000nM;
- Cell staining: For live cells, incubate adherent cells with an appropriate amount of working staining solution for 30 minutes (preferably in a cell culture incubator); for fixed cells, after cell fixation, incubate the fixed cells with an appropriate amount of working staining solution for 30 minutes, then wash three times with PBS; after staining, observe with a confocal fluorescence microscope or a fluorescence microscope, set the excitation wavelength to 405 nm; collect signals at 418-500 nm.
Precautions
- Please centrifuge briefly before use, and appropriately adjust the staining concentration and time according to different cell types;
- Store at -20°C and avoid repeated freezing and thawing;
- For your safety and health, please wear a lab coat and disposable gloves during operation;
- This product is only for scientific research purposes.
ZJ25F0926751
危险品化学品经营许可证（带存储）