Lipopolysaccharides, from E. coli O128:B12 is commonly used to stimulate the inflammatory pathway in an infection/inflammation induced preterm animal model. Specific Lipopolysaccharides from E. coli serotypes induce activation of different inflammatory pathways in the neonatal rat brain. Compared with other Escherichia coli, Lipopolysaccharides (O111:B4, O55:B5, O127:B8), Lipopolysaccharides, from E. coli O128:B12 has lower induction efficiency of inflammation. Cub survival rate was 100% after the treatment.