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EnzymoPure™,天然 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
肌酸激酶在能量需求高且波动大的细胞的能量代谢中起着关键作用。这类细胞包括心肌细胞、骨骼肌细胞以及大脑和视网膜的神经组织等。该酶催化磷酸基团从磷酸肌酸可逆地转移到 ADP 上,从而生成 ATP。据测定,该蛋白质的分子量约为 80 kDa,由 2 个亚基组成,每个亚基的分子量为 40 kDa±2000,其中较轻的亚基含量更高。
应用
牛心肌肌酸磷酸激酶已被用于研究缺血心脏是否会刺激内皮细胞生长,还被用于评估高剂量但无毒的膳食铜和硒摄入对小牛代谢的影响。该产品可用于兔肌原纤维蛋白的 tATP 酶测定,通过此测定可评估结合态肌酸激酶(CK)对钙激活的肌球蛋白 ATP 酶的动力学影响。此外,在通过热解气相色谱法测定色氨酸的过程中,该产品还被用于蛋白质样品的酶解。
Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80 kDa Da. It is made up of 2 subunits, each having a molecular weight of 40 kDa ± 2000. The lighter subunit is present in larger amounts.
Applications
Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves. The product has been used for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase. The product has also been used for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.