计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
菌根侵染状况的观察和侵染率的测定,可以用活体镜检法和染色镜检法。活体镜检法的优点是可连续活体观察多次,能及时大体了解菌根发育情况,并可节省植株。缺点是并不十分准确。为了精确检查菌根发育状况、测定侵染率,则需采用染色法。一般要经过根系透明-染色-分色过程。
阿拉丁乳酸品红染色液 (0.01%) 主要乳酸、甘油、酸性品红、水等组成,用于霉菌的染色观察,也可用于丛枝菌根的染色研究。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
1、蒸馏水、FAA固定液、5%~10%KOH溶液、2%的HCl溶液
2、显微镜、、水浴锅
操作步骤(仅供参考):
1、将根段或经过FAA固定的根系剪成长0.5~1.0cm的小段放入试管或其他染色容器,加入5%~10%KOH溶液,放在90°C水浴锅内20~60min。
2、去掉碱液然后用自来水轻轻冲洗根系3次,再加入2%的HCl溶液浸泡酸化5min。
3、去掉酸液后加入乳酸品红染色液(0.01%),再放回90°C水浴锅内20~60min或室温下过夜。
4、加入乳酸分色后即可镜检。
染色结果:
植物细胞不着色(有时中柱着色),真菌组织染成红色。
注意事项:
1、通常草本植物的幼嫩根系透明时间较短,而木本植物的根系则较长。
2、乳酸品红染色液(0.01%)可回收重复再用。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
4、试剂开封后请尽快使用,以防影响后续实验效果。
The observation of mycorrhizal colonization and the determination of colonization rate can be conducted using the vital microscopic examination method and the staining microscopic examination method. The advantage of the vital microscopic examination method is that it allows repeated continuous observation of living samples, enables timely and general understanding of mycorrhizal development status, and helps save plant materials. Its disadvantage is that the results are not very accurate. To accurately examine mycorrhizal development and determine colonization rate, the staining method should be adopted, which generally involves the processes of root clearing, staining and differentiation.
Aladdin Lactofuchsin Staining Solution (0.01%) is mainly composed of lactic acid, glycerol, acid fuchsin and water. It is used for the staining and observation of molds, and also applicable to the staining research of arbuscular mycorrhizae. This reagent is for research use only and is not intended for clinical diagnosis or any other purposes.
Self-prepared Materials:
1. Distilled water, FAA fixative, 5%–10% KOH solution, 2% HCl solution
2. Microscope, water bath
Operating Procedures (For Reference Only):
1. Cut root segments or roots fixed with FAA into small pieces of 0.5–1.0 cm in length, place them into a test tube or other staining container, add 5%–10% KOH solution, and incubate in a water bath at 90 °C for 20–60 minutes.
2. Discard the alkaline solution, then rinse the roots gently with tap water for 3 times, and subsequently soak them in 2% HCl solution for acidification for 5 minutes.
3. Discard the acid solution, add lactofuchsin staining solution (0.01%), and incubate again in a water bath at 90 °C for 20–60 minutes or overnight at room temperature.
4. Add lactic acid for differentiation and then proceed with microscopic examination.
Staining Results:
Plant cells remain unstained (sometimes the stele is stained), while fungal tissues are stained red.
Precautions:
1. Generally, the clearing time required for the tender roots of herbaceous plants is relatively short, whereas that for the roots of woody plants is longer.
2. The 0.01% lactofuchsin staining solution can be recovered and reused.
3. For your safety and health, please wear a lab coat and disposable gloves during the operation.
4. Please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L1508766 |
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