计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
为了观察病叶内、外部病菌及其他并组织薄片,可采用组织整体透明处理后,可看清楚表生病菌和组织内部病菌,常用的组织整体透明方法有乳酚油、乳酸酚棉蓝、水合氯醛、乳酚油-水合氯醛、水合氯醛-苯酚、乳酸、吡啶等,其中水合氯醛是最常用的方法。
阿拉丁乳酸透明液主要由乳酸和水组成,呈酸性,该产品透明所需时间较长。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
1、蒸馏水、乳酸酚棉蓝染色液、甘油浮载剂
2、梯度乙醇、二甲苯或环保脱蜡透明液、加拿大树胶
3、镊子、显微镜、载玻片
操作步骤(仅供参考):
1、将小块病叶用水洗干净。
2、用镊子将病叶投入乳酸透明液中浸泡2~5天,充分透明。
3、镊子取出组织用蒸馏水清洗,放置于载玻片上。
4、组织中有颜色的菌丝体和子实体可被观察到;没有颜色的透明组织可用乳酸酚棉蓝染色后经脱水封固再观察。
5、滴加甘油浮载剂后盖片观察。
6、若需制成永久玻片,应梯度乙醇脱水,二甲苯或脱蜡透明液处理,并用加拿大树胶封固。
注意事项:
1、乳酸透明液呈酸性,避免直接接触皮肤。
2、乳酸透明病组织所需时间较长,应注意透明充分。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
4、试剂开封后请尽快使用,以防影响后续实验效果。
In order to observe the pathogens inside and outside the diseased leaves as well as other tissue sections, the entire tissue can be subjected to clearing treatment, which allows for clear visualization of both epiphytic pathogens and those residing within the tissue. Common methods for whole tissue clearing include the use of lactophenol, lactophenol cotton blue, chloral hydrate, lactophenol-chloral hydrate, chloral hydrate-phenol, lactic acid, pyridine, etc. Among these, the chloral hydrate method is the most frequently used.
Aladdin Lactic Acid Clearing Solution is mainly composed of lactic acid and water, and it is acidic. This product requires a relatively long time to achieve tissue clearing. This reagent is for research use only and is not intended for clinical diagnosis or any other purposes.
Self-prepared Materials:
1. Distilled water, lactophenol cotton blue staining solution, glycerol mounting medium
2. Gradient ethanol, xylene or environment-friendly dewaxing clearing solution, Canada balsam
3. Tweezers, microscope, glass slides
Operating Procedures (For Reference Only):
1. Rinse small pieces of diseased leaves thoroughly with water.
2. Use tweezers to immerse the diseased leaves in lactic acid clearing solution and soak for 2–5 days to achieve complete clearing.
3. Take out the tissues with tweezers, rinse them with distilled water, and place them on glass slides.
4. Pigmented mycelia and fruiting bodies in the tissues can be observed directly; for non-pigmented and transparent tissues, staining with lactophenol cotton blue followed by dehydration and mounting is required before observation.
5. Add a drop of glycerol mounting medium, cover with a coverslip, and proceed with microscopic observation.
6. If permanent slides are to be prepared, perform dehydration with gradient ethanol, treat with xylene or dewaxing clearing solution, and seal the slides with Canada balsam.
Precautions:
1. Lactic acid clearing solution is acidic; avoid direct contact with the skin.
2. Lactic acid requires a relatively long time to clear diseased tissues, so ensure that the clearing process is thorough.
3. For your safety and health, please wear a lab coat and disposable gloves during the operation.
4. Please use the reagent as soon as possible after opening to prevent compromising subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L1508769 |
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