计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
结缔组织狭义上是指其含有的三种纤维:胶原纤维、网状纤维、弹力纤维,而胶原纤维(collagenfiber)是分布最广、含量最多的一种纤维。Masson三色染色又称马松染色,是结缔组织染色中最经典的一种方法,是胶原纤维染色权威而经典的技术方法,所谓三色染色通常是指染胞核和能选择性的显示胶原纤维和肌纤维;该法染色原理与阴离子染料分子的大小和组织的渗透有关:分子的大小由分子量来体现,小分子量易穿透结构致密、渗透性低的组织;而大分子量则只能进入结构疏松的、渗透性高的组织。
丽春红酸性品红染色液是Masson三色染色的有效成分之一,主要用于肌纤维的染色,染色后呈红色。由丽春红2R、酸性品红等组成。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、切片常规脱蜡至水,蒸馏水洗1min。
2、入丽春红酸性品红染色液染色10min,蒸馏水稍微冲洗。
3、按要求进行下游实验,可95%乙醇快速脱水,无水乙醇脱水3次,每次5~10s,二甲苯透明3次,每次1~2min,中性树胶封固。
染色结果:
胞浆、肌肉、红细胞:红色
注意事项:
1、切片脱蜡应尽量干净。
2、固定起着重要的作用,使用不同的固定液可延或缩短染色时间。
3、注意密闭保存,否则有效成分挥发会导致染色效率下降。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
In a narrow sense, connective tissue contains three types of fibers: collagen fibers, reticular fibers, and elastic fibers. Among them, collagen fibers are the most widely distributed and abundant fiber type. Masson's Trichrome Staining, also known as Masson staining, is the most classic method for connective tissue staining and an authoritative and standard technique for collagen fiber staining. Trichrome staining generally stains nuclei and selectively demonstrates collagen fibers and muscle fibers. The staining principle is related to the molecular size of anionic dyes and tissue permeability: Molecular size is reflected by molecular weight. Small molecules easily penetrate dense tissues with low permeability, whereas large molecules can only enter loose tissues with high permeability.
Ponceau Acid Fuchsin Staining Solution is one of the active components in Masson's Trichrome Staining, mainly used for staining muscle fibers, which appear red after staining. It is composed of Ponceau 2R, acid fuchsin, etc. This reagent is for research use only. Not for clinical diagnosis or other purposes.
Operating Procedures (For Reference Only):
1. Deparaffinize sections routinely to water, then rinse in distilled water for 1 min.
2. Stain in Ponceau Acid Fuchsin Staining Solution for 10 min, then rinse briefly with distilled water.
3. Perform downstream procedures as required. Rapidly dehydrate in 95% ethanol, then dehydrate in absolute ethanol 3 times, 5–10 s each. Clear in xylene 3 times, 1–2 min each. Mount with neutral balsam.
Staining Results:
Cytoplasm, muscle, red blood cells: Red
Precautions:
1. Section deparaffinization should be as thorough as possible.
2. Fixation plays an important role. Different fixatives may prolong or shorten the staining time.
3. Store tightly sealed; otherwise, volatilization of active components will reduce staining efficiency.
4. For your safety and health, wear laboratory coat and disposable gloves during operation.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L1516051 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →