溶菌酶(lysozyme)又称胞壁质酶(muramidase),是一种能水解致病菌中黏多糖的碱性酶。其作用机制为破坏细菌细胞壁中N-乙酰胞壁酸和N-乙酰氨基葡糖之间的β-1,4糖苷键,使细胞壁不溶性黏多糖分解成可溶性糖肽,导致细胞壁破裂、内容物逸出,最终使细菌溶解。
溶菌酶溶液(10 mg/mL)由溶菌酶和TRIS等成分组成,pH值约为8.0,主要用于溶解细菌细胞壁,方便后续质粒DNA的提取工作。
本试剂仅用于科研领域,不适用于临床诊断或其他用途。
产品特点
抗热性强,能在一定温度范围内保持活性;
对有机溶剂不敏感,有机溶剂无法使其完全失活;
活力稳定,能长期保持良好的酶解效果;
具有抗菌性,抗菌谱较广泛;
兼具有效的防腐作用,可辅助保护实验样本。
操作步骤(仅供参考)
根据实验具体要求进行操作,一般按细菌悬液与溶菌酶溶液体积比6:1~10:1的比例添加本试剂。
注意事项
若每次使用量较小,可将试剂适当分装后使用,避免反复冻融影响活力;
为保障实验人员的安全和健康,操作时请穿戴实验服并佩戴一次性手套;
试剂开封后请尽快使用,防止活力下降,影响后续实验效果。
Lysozyme, also known as muramidase, is an alkaline enzyme capable of hydrolyzing the mucopolysaccharides present in pathogenic bacteria. Its mechanism of action involves breaking the β-1,4 glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine in the bacterial cell wall. This breaks down the insoluble mucopolysaccharides in the cell wall into soluble glycopeptides, leading to cell wall rupture, leakage of cellular contents, and ultimately, bacterial lysis.
The lysozyme solution (10 mg/mL) is composed of lysozyme and TRIS among other components, with a pH of approximately 8.0. It is primarily used to lyse bacterial cell walls to facilitate subsequent plasmid DNA extraction procedures.
This reagent is intended for research purposes only and is not suitable for clinical diagnosis or other uses.
Product Features
High heat resistance, maintaining activity within a certain temperature range.
Insensitive to organic solvents, which cannot completely inactivate it.
Stable activity, ensuring long-term effective enzymatic effects.
Exhibits antibacterial properties with a broad antibacterial spectrum.
Possesses effective preservative qualities, aiding in the protection of experimental samples.
Operating Procedure (For reference only)
Follow specific experimental requirements. Generally, add this reagent at a volume ratio of bacterial suspension to lysozyme solution ranging from 6:1 to 10:1.
Precautions
If using small volumes each time, it is advisable to aliquot the reagent appropriately to avoid repeated freeze-thaw cycles that may affect activity.
To ensure the safety and health of experimenters, please wear a lab coat and disposable gloves during operation.
Use the reagent promptly after opening to prevent a decrease in activity that could affect subsequent experimental results.