计算溶液所需的质量、体积或浓度。
脂肪固定液
有货
为什么选择此级别
BioReagent,即用型 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
组织病理学中，组织必须先经过及时有效的固定才能完成随后的包埋及切片。组织固定的作用在于保持组织､细胞的固有形态和结构，防止细菌的腐蚀和组织细胞的自溶，保存细胞内固有的物质，使细胞内蛋白质凝固，尽量减少或终止内源性及外源性酶的反应，使细胞或组织基本上保持与生活时的物质一样，还能使组织硬化，便于组织的修切。对于某些具有传染性的标本，能防止疾病的扩散；保存好大体标本。最后，固定还可增强染色的作用。
脂肪组织含有大量脂滴，如果没有及时有效的固定，在石蜡制片过程中容易出现脂肪细胞膜破损，导致脂肪细胞结构不完整。在冰切制片及脂肪染色过程中，容易出现脂滴移位现象。
脂肪固定液专门用于脂肪组织的固定，能够有效及时的固定脂肪，解决上述制片问题，制备出质量良好的石蜡切片及冰冻切片。本产品主要成分为甲醛､冰醋酸及少量乙醇。
该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
将新鲜脂肪组织取下后迅速放入脂肪固定液中，固定液体积以完全浸没组织且组织能自由移动为准，一般应为组织体积的 10 倍以上。因脂肪组织密度小，刚放入固定液中组织易漂浮在液面上，可在固定容器表面塞入适量脱脂棉球，使脂肪组织悬浮在固定液中，直至组织沉入固定液底部，使组织充分固定。一般室温固定 12‑24 h。
注意事项:
1、请在通风良好的环境下使用，并做好防护，避免吸入。
2、固定液体积需充足，装固定液的容器不能太小，确保组织能自由悬浮在固定液中，不要受到积压，否则会影响组织结构完整性。
3、用完后请及时拧紧瓶盖，防止有效成分挥发。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
In histopathology, tissues must undergo timely and effective fixation prior to subsequent embedding and sectioning. Tissue fixation functions to preserve the inherent morphology and structure of tissues and cells, prevent bacterial erosion and autolysis of tissue cells, retain intrinsic cellular substances, coagulate intracellular proteins, minimize or terminate endogenous and exogenous enzymatic reactions, and keep cellular or tissue substances largely consistent with their in‑vivo state. It also hardens tissues to facilitate trimming. For certain infectious specimens, fixation prevents pathogen spread and preserves gross specimens. Finally, fixation improves staining performance.
Adipose tissue contains abundant lipid droplets. Without timely and effective fixation, adipocyte membranes are prone to rupture during paraffin‑embedded tissue preparation, resulting in incomplete adipocyte architecture. Lipid‑droplet displacement frequently occurs during cryosection preparation and fat‑specific staining.
Adipose‑tissue Fixative is specially formulated for fixation of adipose tissue. It enables prompt and effective fat fixation to resolve the above‑mentioned histological‑preparation issues and produce high‑quality paraffin sections and frozen sections. This product mainly consists of formaldehyde, glacial acetic acid and a small amount of ethanol.
This reagent is for research use only and is not intended for clinical diagnosis or other applications.
Operating Procedures (For Reference Only)
Immediately place freshly‑harvested adipose tissue into adipose‑tissue fixative. The fixative volume shall fully immerse the tissue and allow free movement of the specimen, generally no less than 10 times the tissue volume. Owing to the low density of adipose tissue, specimens tend to float on the liquid surface upon immersion. Place an appropriate amount of absorbent cotton on top inside the container to submerge the tissue until it sinks to the bottom for adequate fixation. Fix at room temperature for 12‑24 hours in general.
Precautions
1. Use in a well‑ventilated environment with proper personal protection and avoid inhalation.
2. Use sufficient volume of fixative and choose an adequately‑sized container to ensure free suspension of tissue without compression; otherwise, tissue structural integrity will be compromised.
3. Tighten the cap promptly after use to prevent volatilization of active ingredients.
4. Wear a lab coat and disposable gloves for your safety and health during operation.
5. Use the reagent as soon as possible after opening to avoid compromised experimental performance.
规格
规格或纯度
BioReagent, 即用型
稳定性与储存
Store at RT long term (12 months).
英文名称
Lipid Fixation Solution
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）