提供 ≥97%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Leptin (aka OB protein, or Obesity factor protein) is a 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.|Leptin (human), (recombinant) is produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of approximately 16 kDa. The Leptin protein is purified by proprietary chromatographic techniques.|Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Product description
Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Human Leptin is a 16.0 kDa protein containing 147 amino acid residues.
Source
E.coli
Synonyms
Obesity protein (OB)
AA Sequence
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
Purity:
≥ 97% by SDS-PAGE gel
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg
Accession Number
P41159
Gene ID
3952
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Leptin (aka OB protein, or Obesity factor protein) is a 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.|Leptin (human), (recombinant) is produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of approximately 16 kDa. The Leptin protein is purified by proprietary chromatographic techniques.|Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Product description
Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Human Leptin is a 16.0 kDa protein containing 147 amino acid residues.
Source
E.coli
Synonyms
Obesity protein (OB)
AA Sequence
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
Purity:
≥ 97% by SDS-PAGE gel
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg
Accession Number
P41159
Gene ID
3952
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
P264: 处理后要彻底洗手。
C2325742
危险品化学品经营许可证（带存储）