Catalase-like activity of Co2+-MOF/CNTs nanocomposite for in situ electrochemical sensing of H2O2 in living cells

A flexible electrochemical sensing platform on the basis of carbon cloth modified with zeolite imidazole framework-67/carbon nanotubes nanocomposite with catalase-like activity was developed for in situ and real-time tracking of H 2 O 2 produced by living cells. The obtained sensor exhibits excellent electrochemical performance for detecting H 2 O 2 with a broad linear response range from 1 µM to 5.2 mM and a detection limit of 0.3 µM. Owing to the favorable biocompatibility and mechanical stability of the prepared electrodes, living cells can be proliferated well on their surfaces, thereby achieving in situ and real-time profiling of endogenous H 2 O 2 flux from normal and cancer cells. This work proposed a novel sensing platform for extracellular analysis, providing an innovative tool for the dynamic monitoring of biomarkers generated by cellular metabolism in cell biology. Graphical abstract The alternative text for this image may have been generated using AI.