BIOSENSORS & BIOELECTRONICS
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2019
·
IF 9.518
Yujie Yu,Chao Yu,Rufei Gao,Jun Chen,Hangtian Zhong,Yilin Wen,Xingduo Ji,Jiahao Wu,Junlin He
We fabricate a novel electrochemical biosensor based on the specific thymine-Hg 2+ -thymine (T-Hg 2+ -T) base pair for the highly sensitive detection of mercury ions (Hg 2+ ) and utilize toluidine blue (TB) as a redox indicator that is combined with a hybridization chain reaction (HCR) for signal amplification . The dandelion-like CuO (D-CuO) microspheres that were assembled using Au nanoparticles were first introduced as support materials, which produced more active sites for the thiolated probe (P1) combination. Then, the presence of Hg 2+ induced P1 to hybridize with the other oligonucleotide (P2) through Hg 2+ -mediated T-Hg 2+ -T complexes. In addition, the partial sequence of P2 acted as an initiator sequence, which led the two hairpin DNA (H1 and H2) strands to collectively form the extended double-strand DNA through the HCR process on the electrode surface . TB was employed to interact with the double strands and produce an efficient electrochemical signal. The proposed strategy combined the amplification of the HCR and the inherent redox activity of TB and utilized D-CuO/Au composites, which exhibited high sensitivity for Hg 2+ determination. Under the optimum conditions, the proposed biosensor showed a prominent response for Hg 2+ , including a linear range from 1 pM to 100 nM and a detection limit of 0.2 pM (S/N = 3). Moreover, the new biosensor proved its potential application for trace Hg 2+ determination in environmental water samples.
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ADVANCED FUNCTIONAL MATERIALS
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2024
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IF 18.5
Ruyan Xie,Shuzhe Zhang,Yanhua Song,Xiaozhen Zhang,Shihou Sheng,Haifeng Zou
The optical properties of quantum dots are significantly influenced by photoactivation. However, the progress in the development of photoactivation carbon dot (CDs) is still stagnant. Herein, a method for preparing photoactivated CDs/NiFe 2 O 4 by introducing affluent defects onto the surface of CDs through modification with NiFe 2 O 4 , extremely filling the gap of photoactivated CDs is proposed. Interestingly, a fantastic fluorescence detection strategy in a unique “OFF-ON-OFF” mode, combined with Ag + -assisted fluorescence enhancement, to establish a dual-channel regulation mechanism for ultra-sensitive detection of Hg 2+ is first proposed. By increasing surface traps and non-radiative recombination, defect-induced manipulation on the surface of CDs creates favorable conditions for enhancing photoactivation capacity. Unexpectedly, the CDs/NiFe 2 O 4 shows a 25-fold enhancement in fluorescence intensity after exposure to UV-lamp. Compared to CDs/NiFe 2 O 4 , Ag + -assisted photoactivation CDs/NiFe 2 O 4 exhibits superior selectivity and sensitivity for Hg 2+ detection, with low detection limits of 0.7 nM. Moreover, TiO 2 @CDs/NiFe 2 O 4 catalyst is successfully prepared by anchoring CDs/NiFe 2 O 4 onto the surface of TiO 2 , which demonstrates efficient degradation of tetracycline within 90 min under visible light irradiation, with a degradation rate of 98.3%. This work first provides a novel defects modification strategy for developing highly active photoactivated CDs technology that is significant for future environmentally sensitive applications.
研究领域:
材料科学
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环境科学
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分析化学
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ANALYTICAL CHEMISTRY
·
2016
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IF 5.886
Wang Ren,Ying Zhang,Hong Guo Chen,Zhong Feng Gao,Nian Bing Li,Hong Qun Luo
A novel signal-on and label-free resonance Rayleigh scattering (RRS) aptasensor was constructed for detection of Hg2+ based on Hg2+-triggered Exonuclease III-assisted target recycling and growth of G-quadruplex nanowires (G-wires) for signal amplification. The hairpin DNA (H-DNA) was wisely designed with thymine-rich recognition termini and a G-quadruplex sequence in the loop and employed as a signal probe for specially recognizing trace Hg2+ by a stable T–Hg2+–T structure, which automatically triggered Exonuclease III (Exo-III) digestion to recycle Hg2+ and liberate the G-quadruplex sequence. The free G-quadruplex sequences were self-assembled into guanine nanowire (G-wire) superstructure in the presence of Mg2+ and demonstrated by gel electrophoresis. The RRS intensity was dramatically amplified by the resultant G-wires, and the maximum RRS signal at 370 nm was linear with the logarithm of Hg2+ concentration in the range of 50.0 pM to 500.0 nM (R = 0.9957). Selectivity experiments revealed that the as-prepared RRS sensor was specific for Hg2+, even coexisting with high concentrations of other metal ions. This optical aptasensor was successfully applied to identify Hg2+ in laboratory tap water and river water samples. With excellent sensitivity and selectivity, the proposed RRS aptasensor was potentially suitable for not only routine detection of Hg2+ in environmental monitoring but also various target detection just by changing the recognition sequence of the H-DNA probe.
研究领域:
分析化学
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环境科学
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生物传感技术
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ANALYTICA CHIMICA ACTA
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2025
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IF 6
Zifei Wu,Hao Ding,Kailai Xu,Chenghui Li,Xiandeng Hou
Background due to the toxicity and widespread distribution of inorganic mercury ions (Hg 2+ ), it is a crucial task for researchers to develop simple methods for field analysis of water samples for trace Hg 2+ . Result a colorimetric and ratiometric fluorescent dual-mode assay based on bifunctional UiO–(COOH) 2 –NH 2 /Eu and gold nanocomplexes (AuNPs@UiO–(COOH) 2 –NH 2 /Eu) was constructed for the sensitive, selective and potentially on-site determination of Hg 2+ . The incorporation of Hg 2+ resulted in the formation of gold amalgam, which enhanced peroxidase-like activity of AuNPs@UiO–(COOH) 2 –NH 2 /Eu, accelerating the oxidization of zymolyte 3,3′,5,5′-tetramethylbenzidine (TMB). Concurrently, the color of the reaction solution turned blue, and the RGB (red, green, and blue) of the solution color changed accordingly. The fluorescence at 618 nm was almost unchanged with the increase of Hg 2+ concentration, whereas the fluorescence at 460 nm was obviously quenched due to the inner filter effect (IFE). Under UV irradiation, the fluorescence emission of the sensing system changed from blue to red, enabling easy visual detection. The probe showed great linearity over a wide range of 20–1200 nM (colorimetric) and 50–4000 nM (fluorescent), with limits of detection (LODs) of 8 nM and 3 nM, respectively. Significance the proposed method was successfully applied to the determination of trace Hg 2+ in tap water and groundwater, while the use of a smartphone enables its simplicity and portability. The results show that the colorimetric and ratiometric fluorescent dual-mode method based on AuNPs@UiO–(COOH) 2 –NH 2 /Eu can achieve rapid visual determination of Hg 2+ potentially useful for on-site analysis.
研究领域:
分析化学
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环境科学
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纳米技术
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RSC Advances
·
2013
·Vol. 3
·期 46
·24618-24624
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IF 2.562
Dongyue Su,Xin Yang,Qingdong Xia,Fang Chai,Chungang Wang,Fengyu Qu
This paper describes the sensing application of water-dispersible thioctic acid functionalized gold nanoparticles (TA-GNPs) as sensors for the colorimetric detection of Hg2+ ion in water samples. The state and stability of thioctic acid decorated gold nanoparticles in pH range (pH = 4–11) were evaluated by UV/vis spectra. The thioctic acid modified GNPs could be induced to aggregate quickly in the presence of Hg2+, especially after adding a solution of 0.05 M NaCl. The detection of Hg2+ by TA-GNPs at different pH values (pH = 5, 7, 9, 11) was also investigated to optimize the experimental conditions in detail. The result indicated that the TA-GNPs at pH 5 were the most optimal choice for detecting Hg2+. The detection results of Hg2+ could be determined by the naked eye or a UV-vis spectrophotometer. The detection of Hg2+ could be realized with a detection limit of 10 nM over a long range (10 nM to 20 μM). Additionally, the selectivity of this method has been investigated using other metal ions. The TA-GNPs can selectively bind Hg2+ over other metal ions (Pb2+, Mg2+, Zn2+, Ni2+, Cu2+, Co2+, Ca2+, Mn2+, Fe2+, Cd2+, Ba2+, Ag+ and Cr3+), leading to prominent color change. This will provide a simple and effective colorimetric sensor (no DNA) for on-site and real-time Hg2+ ion detection. More importantly, this probe was also applied to determine the Hg2+ in lake samples, and the results demonstrate low interference and high sensitivity.
研究领域:
分析化学
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环境科学
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纳米技术
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International Journal of Electrochemical Science
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2023
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IF 1.5
Li Sun,Buhong Gao,Weina Jiang,Li Xu,Wen Lu,Shilong Yang,Dong Jiang,Jichao Chen,Huayu Xue,Jiuzhou Shi
Isorhamnetin as a natural product has excellent biological activity, but there are few reports of it in electrochemical applications. In this paper, we developed a neoteric electrochemical sensor for sensitive detection of mercury ions (Hg 2+ ) for the first time using glassy carbon (GC) electrode which was modified with isorhamnetin (ISO) and graphene oxide (GO). The electrochemical behaviors of the fabricated GC electrode were characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under optimal conditions, the linear range to detect Hg 2+ with DPV was from 1.0×10 -6 to 1.0×10 -4 M with limit detection of 2.3×10 -8 M (S/N=3). In addition, it also showed excellent selectivity of Hg 2+ with comparison to several inorganic salt ions and other metal ions. We also report the application for Hg 2+ detection in real water samples with a good recovery (92.1-104.7%). In conclusion, isorhamnetin can be applied in the electrochemical field successfully with extraordinary results.
研究领域:
分析化学
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环境科学
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生物医学工程
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Materials Advances
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2022
·Vol. 3
·期 8
·3593-3599
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IF 0
Shanshan Xing,Chunqiu Xia,Xinyi Liu,Liangqia Guo,Fengfu Fu
A simple and green one-step method for the simultaneous exfoliation and functionalization of few-layered MoS2 nanosheets was developed via the ultrasonic treatment of bulk MoS2 powder in sodium phytate-containing aqueous solution. The as-prepared MoS2 nanosheets show high stability, low cytotoxicity and high adsorption capacity for Hg2+ in aqueous solution. The maximum adsorption capacity for Hg2+ is about 7.5 times and 3.7 times that of bulk MoS2 powder and unfunctionalized MoS2 nanosheets, respectively. The adsorption kinetics and thermodynamics indicate that the adsorption of Hg2+ is a monolayer chemical adsorption. Finally, the as-prepared MoS2 nanosheets were applied for the removal of Hg2+ in HepG2 cells and show a similar detoxification effect as that of the common antidote meso-2,3-dimercaptosuccinic acid, indicating the potential application of MoS2 nanosheets for biological detoxification.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
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2019
·Vol. 21
·期 6
·2919-2928
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IF 3.567
Kang-Tao Liu,Shi-Yong Ran
The metal ion–DNA interaction is key to biochemical processes and has applications in areas such as metal ion sensors and DNA nanomachines. For example, the formation of the T–Hg2+–T structure has been used in technologies such as DNA-based mercuric ion sensors. Though the interaction is widely used for practical purposes, the underlying mechanism has not been fully understood. In the present study, we used magnetic tweezers to explore the interactions between λ-DNA and two metal ions, Hg2+ and Cd2+, at the single-molecule level. Both metal ions caused considerable DNA conformational changes. The resulting DNA compaction dynamics were related to the ion concentration and the exerted force. The increase in the ion concentration promoted DNA compaction, whereas exerting greater forces inhibited this process. Application of a high force generated two-stage dynamics of the Hg2+–DNA interaction. However, at a sufficiently high Hg2+ concentration, a lower force led to a three-stage process. In contrast, the curves of the binding of Cd2+ ions to DNA had a stepwise pattern. Both the AFM scanning results and the single-molecule measurements confirmed that Hg2+ influences the DNA conformation in a more pronounced manner than Cd2+. The multistage Hg2+–DNA interaction was considered to be a result of the different binding mechanisms, including the mismatched base-pair formation. A model was then proposed to explain the peculiar dynamics.
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EUROPEAN POLYMER JOURNAL
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2024
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IF 5.8
Hong-Hui Shu,Hui-Qin Zhang,Sheng-Li Han,Yun Liu,Xiu-Qin Fang,Cheng-Mei Liu
Petroleum-derived polythioureas (PTUs) are typical functional polymers that have been applied as essential materials in the optoelectric and environmental fields. The design and preparation of sustainable PTUs are the current focus in this field. In the present study, we first proposed that PTUs can be prepared from bisfuran diamines by solution polymerisation with CS 2 without any catalyst. The diamine monomers were prepared from renewable furfurylamine, which enabled the final PTUs to be sustainable. The highest molecular weight of the resulting PTUs was 86.64 kg/mol, with a polydispersity of 1.32. The glass transition temperature ( T g ) of PTU can be tuned by changing the spacer of monomers. All synthesised PTUs showed excellent thermal stability, and the char yield at 800 °C was > 40 %. Attractively, these PTUs are excellent Hg 2+ ion capturers due to their thiourea structure.
研究领域:
材料科学
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环境科学
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ANALYST
·
2012
·Vol. 137
·期 22
·5398-5402
·
IF 4.23
Qun-bo Mei,Yuan-hui Guo,Bi-hai Tong,Jie-Na Weng,Bin Zhang,Wei Huang
A new phosphorescent chemosensor for Hg2+ and acetonitrile (MeCN) based on iridium(III) complex Ir(dpp)2(dtc) (Ir1, dppH = 4,6-diphenylpyrimidine, dtcH = diethyl dithiocarbamic acid) was realized. Upon addition of a tetrahydrofuran (THF) solution of Hg2+, the dichloromethane (DCM) solution of Ir1 gave a visual color change and significant fluorescent quenching. When MeCN was added, a new fluorescent emission emerged, which constituted a selective MeCN phosphorescent chemosensor. Complex Ir1 has been developed as an AND logic gate with Hg2+ and MeCN as inputs.
研究领域:
分析化学
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配位化学
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MICROCHEMICAL JOURNAL
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2022
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IF 5.304
Dehong Bai,Ziyu Xue,Peiqing Guo,Mingzhu Qiu,Xuefang Lei,Yujin Li,Chengjie Ma,Dongxia Zhang,Xibin Zhou
Due to the harm of metal mercury to the environment and the human body, there is an urgent need for exploring novel, simple, and reliable methods for the detection of Hg 2+ . Herein, a colorimetric method is proposed for the determination of Hg 2+ based on the analyte inducing the aggregation of Glutathione-modified gold nanoflowers (GSH-Au NFs) with activated peroxidase-mimicking activity. Briefly, highly dispersed GSH-Au NFs show negligible enzyme-like activity in water. When Hg 2+ is added, GSH-Au NFs would aggregate rapidly for the interaction of Hg 2+ with the carboxyl and amine groups of GSH. And the aggregates exhibit high peroxidase-mimicking catalytic activity and can catalyze the color reaction of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . Based on these phenomena, an ultrasensitive colorimetric detection method of Hg 2+ has been developed. The linear range of this method is 10–300 nM and the detection limit is down to 3.9 nM, which is much lower than the standard value specified by the US EPA. The accuracy of the method has been verified in water samples, which reveals its great potential in environmental monitoring.
研究领域:
环境科学
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纳米生物学
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分析化学
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JOURNAL OF FLUORESCENCE
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2022
·Vol. 32
·期 5
·1755-1759
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IF 2.525
Nie Jia,Xu Tong,Liu Qian,Yang Chun,Sun Xuping
Hg 2+ is one of the most toxic heavy metal ions that exist in the environment and it forms large numbers of toxic binary compounds. Accurate and rapid detection of the concentration of heavy metal ions is a prerequisite technology to achieve pollution control and prevention. Fluorescent probes have attracted extensive attention because of their high sensitivity, prominent precision, convenient and fast visualization of heavy metals. Herein, we report multi-layered graphitic carbon nitride via a simple thermopolymerization treatment as a very effectual fluorescent probe for sensitive and selective detection of Hg 2+ with a limit of detection as low as 1.14 nM.
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