JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 12
·B1028
·
IF 3.12
Dandan Hu,Yufang Hu,Qingqing Zhang,Pingjian Ran,Xinda Liu,Yudi Zheng,Zhiyong Guo,Sui Wang,Di Wu
Monitoring DNA-related enzymes activity precisely and reliably is absolutely vital to better grasp their roles in the initiation and progression of cancer, thus further exploring their functions in cancer diagnosis and treat. Here, we establish a label-free electrochemical biosensor for investigating both terminal deoxynucleotidyl transferase (TdT) and exonuclease I (Exo I) activity by silver-based square wave stripping voltammetry (SWV). The strategy is achieved by two attractive points in design: a TdT-derived enzymatic synthesis of randomly arrayed C-rich DNA sequence and an Exo I-guided enzymatic degradation. This C-rich DNA-templated silver nanoclusters (AgNCs) serve as the electrochemical signal probe, and a signal on-off modulated process is displayed for the existence of TdT and Exo I, respectively. On account of AgNCs-based square wave stripping voltammetry, testing TdT has a linear concentration range of 0.001 to 5 U/mL with a detection limit of 0.00044 U/mL (S/N = 3). Additionally, Exo I with a detection limit of 0.027 U/mL (S/N = 3) can be analyzed with a linear range of 0.1 to 500 U/mL. The real application of the proposed method in their inhibitors screening is also discussed well. The biosensor provides a powerful idea for the ultrasensitive bio-analysis and shows broad clinical application prospect.
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2020
·Vol. 15
·期 10
·e0240920
·
IF 2.74
Li-Wei Gao,Hong-Tao Zhu,Cai-Yun Liu,Zhi-Xiang Lv,Xiao-Man Fan,Ye-Wang Zhang
As one of the most extensively studied glycosaminoglycan lyases, heparinase I has been used in producing low or ultra-low molecular weight heparin. Its’ important applications are to neutralize the heparin in human blood and analyze heparin structure in the clinic. However, the low productivity and activity of the enzyme have greatly hindered its applications. In this study, a novel Hep-I from Bacteroides cellulosilyticus (BcHep-I) was successfully cloned and heterologously expressed in E . coli BL21 (DE3) as a soluble protein. The molecular mass and isoelectric point (pI) of the enzyme are 44.42 kDa and 9.02, respectively. And the characterization of BcHep-I after purified with Ni-NTA affinity chromatography suggested that it is a mesophilic enzyme. BcHep-I can be activated by 1 mM Ca 2+ , Mg 2+ , and Mn 2+ , while severely inhibited by Zn 2+ , Co 2+ , and EDTA. The specific activity of the enzyme was 738.3 U·mg -1 which is the highest activity ever reported. The K m and V max were calculated as 0.17 mg·mL -1 and 740.58 U·mg -1 , respectively. Besides, the half-life of 300 min at 30°C showed BcHep-I has practical applications. Homology modeling and substrate docking revealed that Gln15, Lys74, Arg76, Lys104, Arg149, Gln208, Tyr336, Tyr342, and Lys338 were mainly involved in the substrate binding of Hep-I, and 11 hydrogen bonds were formed between heparin and the enzyme. These results indicated that BcHep-I with high activity has great potential applications in the industrial production of heparin, especially in the clinic to neutralize heparin.
研究领域:
生物化学
·
分子生物学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2020
·
IF 7.727
Tao Sun,Guangping Zhang,Zhongyuan Guo,Qinjun Chen,Yujie Zhang,Yongchao Chu,Qin Guo,Chao Li,Wenxi Zhou,Yiwen Zhang,Peixin Liu,Hongyi Chen,Haijun Yu,Liping Jiang,Chen Jiang
Chelating Cu from tumors has been verified as an effective and promising strategy for cancer therapy through antiangiogenesis. However, systematic removal Cu by injecting with Cu chelators will result unavoidable side effects, since Cu is indispensable to the body. In this work, a micelle targeting to tumors' newborn vessels based on a polypeptide was developed to co-load DOX and Probe X, which can go through an “OFF-to-ON” procedure to report the Cu + -capture events in vivo in a real-time way by giving near infrared (NIR) fluorescence and photoacoustic signal. By co-delivering antiangiogenesis and chemotherapeutic reagents, the tumor can be significantly suppressed, meanwhile with a low systematic toxicity. Hopefully, this work can offer new insights in designing sophisticated antitumor strategy.
研究领域:
肿瘤学
·
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Li Zhao,Xianzhi Hu,Futing Zi,Shuliang Chen,Huiling Cheng,Peng Yang,Yan Zhang,Yunlong Chen,Yunshu Jiang,Xinrong Li,Yue Lin,Zhujuan Li,Juan Li,Haiwen Wang,Yufang Li
It is desirable to develop a highly stable, cost-effective, and recyclable adsorbent to adsorb Au(I) from thiosulfate solutions. In this study, reliable aminated microspheres (PS–TETA) were synthesized via a one-pot method that profited from active chloromethyl group-induced nucleophilic substitution between chloromethylated polystyrene (PS–Cl) and triethylenetetramine (TETA). These microspheres were used to recover a gold(I) thiosulfate complex. Scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller technique, and X-ray photoelectron spectroscopy were adopted to analyze the surface elements and structure of PS–TETA. Additionally, influencing parameters, such as pH, thiosulfate concentration, and temperature, were investigated. The results indicated that the synthetic process and gold-adsorption behavior of PS–TETA was directly related to the surface chlorine and amine groups. The adsorption process was exothermic and consistent with the Freundlich isotherm model and pseudo-second-order kinetics. Complete adsorption of Au(I) was achieved with a maximum capacity of 23.79 kg t–1. Na2SO3 proved to be an effective desorbent and exhibited a satisfactory adsorption ability of the adsorbent even after five cycles. Anion exchange as a reasonable Au(S2O3)23– adsorption mechanism of PS–TETA is proposed. This study offers a practical method for synthesizing aminated microspheres with excellent properties to recover Au(I) from thiosulfate solutions.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2020
·
IF 1.415
Cai-Yun Liu,Wen-Bin Su,Li-Bin Guo,Ye-Wang Zhang
Heparinase I (Hep I) specifically degrades heparin to oligosaccharide or unsaturated disaccharide and has been widely used in preparation of low molecular weight heparin (LMWH). In this work, a novel Hep I from Bacteroides eggerthii VPI T5-42B-1 was cloned and overexpressed in Escherichia coli BL21 (DE3). The enzyme has specific activity of 480 IU·mg−1 at the optimal temperature and pH of 30 °C and pH 7.5, and the Km and Vmax were 3.6 mg·mL−1 and 647.93 U·mg−1, respectively. The Hep I has good stability with t1/2 values of 350 and 60 min at 30 and 37 °C, respectively. And it showed a residual relative activity of 70.8% after 21 days incubation at 4 °C. Substrate docking study revealed that Lys99, Arg101, Gln241, Lys270, Asn275, and Lys292 were mainly involved in the substrate binding of Hep I. The shorter hydrogen bonds formed between heparin and these residues suggested the higher specific activity of BeHep I. And the minimum conformational entropy value of 756 J·K−1 provides an evidence for the improved stability of this enzyme. This Hep I could be of interest in the industrial preparation of LMWH for its high specific activity and good stability.
研究领域:
生物化学
·
酶学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 33
·16177-16182
·
IF 3.925
Shuyan Xiao,Liang Sun,Jiping Lu,Zhongping Dong
A label-free fluorescent biosensor based on the clenbuterol binding aptamer and the fluorescent intercalator SYBR Green I (SGI) was established for the sensitive and selective detection of clenbuterol. In the absence of clenbuterol, a weak fluorescence signal of SGI was observed. In the presence of clenbuterol, the specific recognition and high-affinity binding of the aptamer with clenbuterol induced a conformational change of the aptamer, and a longer double-stranded DNA was formed. As a result, an enhancement in the fluorescence of SGI could be found. Under optimal conditions, the fluorescence change ratio increased linearly with clenbuterol concentration from 50 nM to 450 nM, with a detection limit of 18.8 nM. In addition, the proposed aptasensor showed good selectivity and was successfully applied for clenbuterol quantification in pork samples with recoveries of 90.37–104.98%.
研究领域:
分析化学
·
食品安全
·
分子生物学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zhexiang Wang,Xinyi Li,Rui Lv,Ruochen Guo,Qinglei Shu,Tianjin Ge,Jun Yan,Zhihao Zhao,Guanghui Wang,Jian Liu
The click chemistry catalyzed by transition metals is the research front line for bioorthogonal therapeutics. However, it demands the use of external ligands to form transition metal–ligand complexes for the catalytic enhancement, thus leading to biosafety concerns on the undesired side effects of the complexes or the additional ligands themselves during therapies. Here we report a pH/GSH-driven inside-out nanoreactor initiating Cu(I)-precursor chelation-promoted bioorthogonal catalysis against malignant tumors. It comparts the precursors (Azide and Alkyne) and Cu(II) safely with disulfide-functionalized hyaluronic acid-polycarprolactone (abbr. PCHP1) polymersome structure. Once sensing the pH/GSH changes typically in tumor microenvironment, our nanoreactor starts with a unique programed releasing, allowing for the inside-out translocation of Azide with the highest priority. Subsequently the Cu(I)-Azide chelation can promote the catalytic reaction to generate drug molecules in a highly efficient, ligand-free manner. The PCHP1 nanoreactor can suppress malignant tumors significantly better than the other control groups in the in vitro and in vivo experiments, while inducing minimal side effects. Our strategy of ligand-free Cu(I)-precursor chelation promoted click chemistry will facilitate the development of bioorthogonal catalytic therapies for clinical translation.
研究领域:
药学
·
肿瘤学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2024
·
IF 3.3
Lizhu Zhang,Wenqian Du,Jiawen Wu,Rongjie Yang,Fen Zhao,Baomin Fan,Zhonghua Xia
A gold-catalyzed sulfonylation of aryl/vinyl iodides to synthesize aryl sulfones facilitated by the ligand-enabled Au(I)/Au(III) redox catalysis was developed. In the reaction, aryl sodium sulfinates or sulphinic acids can react smoothly with aryl/vinyl iodides to directly construct various aryl sulfones. The strong synthetic capabilities of sulfone synthesis are demonstrated by its easily available and handled reagents, good functional group compatibility, and late-stage application of complicated biomolecules. Mechanistic studies suggest that the silver salt plays a crucial role in the transmetalation with the Au(I)/Au(III) intermediate, and the gold complex favors Au–S bond formation over Au–O bond formation.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
PubMed
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2025
·
IF 7.8
Gao Feiyue,Zhou Chuncai,Cao Yachuan,Yang Ke,Li Zhiguo,Li Xin,Liu Guijian
Thallium (Tl) is a typical hypertoxic heavy metal, its accumulation in the water environment can have serious impacts on ecosystems and human health. In this study, a composite material (PC@MnO 2 ) was designed for removing Tl(I) by grafting MnO 2 onto coal tar residue-based porous carbon (PC) via hydrothermal impregnation. δ-MnO 2 with nano flower-like structure was verified to be evenly distributed on PC surface through material characterizations. The adsorption performance was markedly elevated after loading MnO 2 on PC, and the optimal adsorbent (PC@MnO 2 -75) was prepared by hydrothermal process from KMnO 4 (75 mg) using PC (100 mg) as a reducing agent. The adsorption of Tl(I) onto PC@MnO 2 -75 has achieved equilibrium in 90 min, slightly affected by solution pH (4−12), and exhibited an outstanding adsorptive selectivity in the presence of high concentrations of K + , Na + , Ca 2+ , Mg 2+ , Cl - , CO 3 2- , humic acid, and fulvic acid. The pseudo second order kinetic and Freundlich isotherm models were preferably described as the adsorptive characteristics. And the Tl(I) removal was a spontaneous exothermic adsorptive process. Density functional theory (DFT) results manifested that Mn doping enhanced electron transfer rate during adsorption. The highly efficient removal of Tl(I) on PC@MnO 2 -75 was achieved via electrostatic interaction, complexation, and oxidation-adsorption. The porous carbon derived from coal tar residue was engineered into PC@MnO 2 composites for efficient Tl(I) removal, which was in line with the environmental protection concept of sustainable development and green production.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2017
·
IF 3.833
Yumin Leng,Kai Jiang,Wentai Zhang,Yuhui Wang
Metal-mediated interlocking rings won the 2016 Nobel Prize in Chemistry. The metal-directed interlocking rings in macromolecular systems (e.g., proteins) may be similar to the form of Maxwell’s electromagnetic waves; the metal ions may shuttle among the rings in the special environment. To verify this hypothesis, we designed a general approach to synthesize the multicolored gold nanoparticles (GNPs) mediated by Au(I)-directed interlocking rings in proteins. The Au(I) ions shuttled among these interlocking rings in the strong alkaline solution. Through the rapid nucleation method, the multicolored GNPs of different morphology and sizes were synthesized in the multiple honeycombed templates. On the basis of the “three-color” principle of Thomas Young, we extracted the red, green, and blue (RGB) alterations of GNPs to fabricate a visual sensor array for protein discrimination. The fingerprints (ΔRGB) were obtained from the target proteins and fed into computer programs. The proposed sensing platform was also applied to detect lysozyme in human tears with satisfactory results. Importantly, we forecasted that lysozyme could be the effective drug for curing dacryocystitis and nasolacrimal duct obstruction diseases.
研究领域:
化学
·
生物学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2022
·
IF 10.319
Qin Wang,Yao Tong,Meiting Yang,Hangtao Ye,Xiaojuan Liang,Xin Wang,Weidong Xiang
In the current situation of energy shortages, saving energy and reducing consumption are undoubtedly valid methods to relieve the pressure on resources. CsPb X 3 ( X = Cl, Br, and I) perovskites as star inorganic materials have drawn growing attention owing to their unique properties, making them suitable for applications such as light-emitting diodes (LEDs), solar cells, photodetectors, or lasers. This work presents zinc oxide (ZnO) induced self-crystallized CsPb(Br/I) 3 perovskite with a 26% photoluminescence quantum yield (PLQY) prepared by the traditional melt-quenching method. Furthermore, the full width at half maximum (FWHM) (34–44 nm) can be regulated by the ZnO content. In addition, as a downconverter, CsPb(Br/I) 3 perovskite nanocrystals (NCs) realize a bright red backlight that covers ≈ 94% Rec. 2020 standards in the CIE 1931 color space and 126% of National Television System Committee (NTSC). This finding undoubtedly confirms an effective path for fabricating perovskite materials in a green, low-cost, and time-saving way, enriching the knowledge of glass crystallization mechanisms and promoting their applications in wide color gamut backlights.
研究领域:
材料科学
·
光学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Weirui Chen,Weijie Chen,Yimin Luo,Yanqi Feng,Xukai Li,Jing Wang,Ping Li,Liying Bin,Bing Tang,Laisheng Li
g-C 3 N 4 (CN) based catalysts were effective for photocatalytic ozonation (PCO), but their stability during reaction remained a mystery. This study investigated the change on PCO activity and stability of CN induced by F or I doping. Compared with CN, F-CN had the greater VB potential while I-CN had a greater CB position, which both led to their better O 3 activation. F-CN and I-CN PCO showed the greater oxalic acid (OA) degradation than that of CN PCO process. Though the enhanced activity, both F-CN and I-CN demonstrated the lower stability compared with that of CN. Because of the existing of reactive oxygen species (ROS) like •OH, •O 2 - and h vb + , CN, F-CN and I-CN were partially oxidized, generating NO 3 - and organic molecules such as cyanuric acid. h vb + and •OH mainly accounted for the decomposition of three CN. Triazine ring oxidation resulted in internal tearing and edge fracture as well as the formation of O-containing groups on all CN-based catalysts. O contents in catalysts initially increased and then decreased with reaction time, indicating that surface O-containing groups were unstable. The structural destroy could be eased with the present of organic pollutants. This study deepened the understanding of fate of CN based catalysts during PCO application.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）