BIOMACROMOLECULES
·
2013
·
IF 5.371
Qingchun Zhang,Ke Tan,Yan Zhang,Zhaoyang Ye,Wen-Song Tan,Meidong Lang
In tissue engineering, incorporation of bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable bone formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(ε-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better bone formation was observed in gelatin/rhBMP-2-coated scaffolds. Specifically, the spatial distribution of newly formed bone was more uniform in gelatin-coated scaffolds than in uncoated scaffolds, which displayed preferential bone formation at the periphery. These results collectively demonstrated that gelatin-coating of porous PCL scaffolds is a promising approach for delivering rhBMP-2 to stimulate improved bone regeneration.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Qing Wang,Zhi-Yuan Luo,Mao Ye,Yu-Zhuo Wang,Li Xu,Zhi-guo Shi,Lanying Xu
The zirconia-coated silica (ZrO 2 /SiO 2 ) material was obtained by coupling layer-by-layer (LbL) self-assembly method and sol–gel technology, to take dual advantages of the suitable porous structure of SiO 2 and basic resistance of ZrO 2 . Adenosine 5′-monophosphate (5′-AMP) was then self-assembled onto ZrO 2 /SiO 2 via Lewis acid–base interaction, generating 5′-AMP-ZrO 2 /SiO 2 . The chromatographic properties of 5′-AMP-ZrO 2 /SiO 2 were systemically studied by evaluating the effect of acetonitrile content, pH and buffer concentration in the mobile phase. The results demonstrated that the 5′-AMP-ZrO 2 /SiO 2 possessed hydrophilic interaction chromatographic (HILIC) property comprising hydrophilic, hydrogen-bonding, electrostatic and ion-exchange interactions. For basic analytes, the column efficiency of ZrO 2 /SiO 2 and 5′-AMP-ZrO 2 /SiO 2 was superior to the bare ZrO 2 , and different selectivity was obtained after the introduction of 5′-AMP. For acidic analytes, good resolution was obtained on 5′-AMP-ZrO 2 /SiO 2 while the analysis failed on the bare ZrO 2 column owing to strong adsorption. Hence, the proposed 5′-AMP-ZrO 2 /SiO 2 had great potential in analyzing acidic compounds in HILIC mode. It was an extended application of ZrO 2 based SP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 120
·99418-99424
·
IF 3.84
Jinda Fang,Ke Zhang,Jingwei Jia,Zhengke Wang,Qiaoling Hu
To improve the drug-loading capacity and control the initial release of amphiphilic drug carriers, a series of N-phthaloyl-chitosan-g-(PEO–PLA–PEO) compounds were synthesized with well-defined structures. The self-assembly behavior of copolymers in aqueous solutions was confirmed by various techniques such as fluorescence spectrometry, dynamic light scattering, and transmission electron microscopy. The results demonstrated that the micellization behavior of graft copolymers was different from that of their linear counterparts. The micelle sizes of the graft copolymers could be tuned with chemical composition as well as temperature. Furthermore, when hydrophobic indomethacin was loaded into the micelles, the graft copolymer micelles trapped more indomethacin than PEO–PLA–PEO micelles, facilitating the in vitro control of the initial burst release of the drug. Drug release could be controlled in vitro by simply altering the EO/LA ratio of the grafting chains. The graft copolymer showed low cytotoxicity to 293T cells, indicating its great potential application for drug delivery.
✓ 已验证
产品关联
DOI
高影响因子
NUTRITION RESEARCH
·
2015
·
IF 2.472
Qian Wang,Shuang-ting Wang,Xin Yang,Pan-pan You,Wen Zhang
Myricetin (MyR), a naturally occurring flavonol widely distributed in fruits, vegetables, and medicinal plants , has anticancer, anti-inflammatory, antihyperlipidaemic , and antiobesity activities. In the present study, we hypothesized that the antiobesity property of MyR is mediated via suppression of differentiation of preadipocytes into adipocytes and promotion of lipolysis of mature adipocytes, which effectively decrease the intracellular triglyceride concentration of adipocytes. Accordingly, the aim of this work was to investigate the effects of MyR on adipocyte differentiation and lipolysis in differentiated 3 T3-L1 adipocytes. Our results showed that MyR inhibited differentiation of 3 T3-L1 preadipocytes in a concentration-dependent manner. Myricetin downregulated the mRNA and protein levels of CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor γ , both of which are major adipogenic transcription factors. Furthermore, the mRNA levels of other adipogenesis-related transcription factors, namely, CCAAT/enhancer-binding protein β , sterin regulatory element binding protein 1-c, peroxisome proliferator-activated receptor γ coactivator–1, adipocyte protein 2 , lipoprotein lipase and glucose transporter 4 , were also reduced by MyR treatment. Moreover, MyR significantly inhibited the phosphorylation of extracellular signal–regulated kinase, Jun N-terminal kinase, and p38 during the differentiation process. On the other hand, MyR induced a dose-dependent increase in glycerol release in fully differentiated adipocytes, indicating its stimulatory effect on adipocyte lipolysis. Furthermore, MyR downregulated mRNA level of perilipin A and enhanced the phosphorylation level of extracellular signal–regulated kinase, Jun N-terminal kinase, and p38 during lipolysis. Taken together, these findings indicate that MyR exerts antiobesity activity in adipocytes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2013
·
IF 4.187
Zhiyao Hou,Xuejiao Li,Chunxia Li,Yunlu Dai,Ping’an Ma,Xiao Zhang,Xiaojiao Kang,Ziyong Cheng,Jun Lin
Novel multifunctional poly(ε-caprolactone)-gelatin encapsulating upconversion core/shell silica nanoparticles (NPs) composite fibers as dual drugs delivery system (DDDS), with indomethacin (IMC) and doxorubicin (DOX) releasing in individual release properties, have been designed and fabricated via electrospinning process. Uniform and monodisperse upconversion (UC) luminescent NaYF4:Yb3+, Er3+ nanocrystals (UCNCs) were encapsulated with mesoporous silica shells, resulting in the formation of core/shell structured NaYF4:Yb3+, Er3+@mSiO2 (UCNCs@mSiO2) NPs, which can be performed as DOX delivery carriers. These UCNCs@mSiO2 NPs loading DOX then were dispersed into the mixture of poly(ε-caprolactone) (PCL) and gelatin-based electrospinning solution containing IMC, followed by the preparation of dual drug-loaded composite fibers (DDDS) via electrospinning method. The drugs release profiles of the DDDS were measured, and the results indicated that the IMC and DOX released from the electrospun composite fibers showed distinct properties. The IMC in the composite fibers presented a fast release manner, while DOX showed a sustained release behavior. Moreover, the UC luminescent intensity ratios of 2H11/2/4S3/2–4I15/2 to 4F9/2–4I15/2 from Er3+ vary with the amounts of DOX in the system, and thus drug release can be tracked and monitored by the luminescence resonance energy transfer (LRET) mechanism.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2013
·
IF 2.562
Xiaohong Wang,Guohua Jiang,Zhen Wei,Xia Li,Bolin Tang
The CO 2 stimulus-sensitive nanoparticles based on poly( N , N -dimethylaminoethyl methacrylate)- b -poly styrene (PDMAEMA- b -PS) were prepared via surfactant-free miniemulsion reversible addition–fragmentation chain transfer (RAFT) polymerization. The as-prepared nanoparticles exhibited core–shell structure with about 120 nm in diameter. Their dispersion/aggregation in water can be adjusted by alternatively bubbling of CO 2 and N 2 . Drug release from these nanoparticles can be accelerated (or delayed) by bubbling (or removing) of CO 2 .
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2015
·
IF 3.545
Qing Wang,Yao Long,Lin Yao,Li Xu,Zhi-Guo Shi,Lanying Xu
A mixed-mode chromatographic stationary phase , C18-DTT (dithiothreitol) silica (SiO 2 ) was prepared through “thiol-ene” click chemistry. The obtained material was characterized by fourier transform infrared spectroscope, nitrogen adsorption analysis and contact angle analysis. Chromatographic performance of the C18-DTT was systemically evaluated by studying the effect of acetonitrile content, pH, buffer concentration of the mobile phase and column temperature. It was demonstrated that the novel stationary phase possessed reversed phase liquid chromatography (RPLC)/hydrophilic interaction liquid chromatography (HILIC) mixed-mode property. The stop-flow test revealed that C18-DTT exhibited excellent compatibility with 100% aqueous mobile phase. Additionally, the stability and column-to-column reproducibility of the C18-DTT material were satisfactory, with relative standard deviations of retention factor of the tested analytes (verapamil, fenbufen , guanine , tetrandrine and nicotinic acid) in the range of 1.82–3.72% and 0.85–1.93%, respectively. Finally, the application of C18-DTT column was demonstrated in the separation of non-steroidal anti-inflammatory drugs, aromatic carboxylic acids , alkaloids, nucleo-analytes and polycyclic aromatic hydrocarbons . It had great resolving power in the analysis of various compounds in HILIC and RPLC chromatographic conditions and was a promising RPLC/HILIC mixed-mode stationary phase.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
·
2016
·
IF 3.155
Jihui Wang,Yuan Liang,Luyang Jian,Jingwei Zhang,Shuai Liang,Shan Xiao,Bingnan Liu,Han Wang
Obesity has become a major health problem which is related with high-trans fatty acids diet. Adipogenic differentiation of adipose tissue-derived stromal cells (ADSCs) plays an important role in the development of adipose tissue . In order to determine the effect of trans fatty acids on adipogenic differentiation in ADSCs, cells were treated with linoelaidic acid, as well as linoleic acid and linolenic acid . We found that linoelaidic acid significantly increased the lipid droplet formation and triglyceride content compared with linoleic acid and linolenic acid. Linoelaidic acid also down-regulated the levels of β-catenin in cells and inhibited the accumulation of β-catenin in cell nuclei . Lithium chloride , an activator of Wnt/β-catenin pathway, antagonized the enhancement of linoelaidic acid on adipogenesis and up-regulated the levels of β-catenin in ADSCs. These results indicated that linoelaidic acid could enhance the adipogenic differentiation in ADSCs in vitro , which is partly due to the suppression of Wnt/β-catenin pathway.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 27
·16401-16409
·
IF 3.108
Xian Sun,Yingping Luo,Liwei Huang,Bo-Yang Yu,Jiangwei Tian
Multidrug resistance (MDR) is a major obstacle to the success of cancer chemotherapy, which is closely associated with the overexpression of P-glycoprotein (P-gp) and the endolysosomal capture of drug. Therefore, to explore a strategy that could down-regulate P-gp expression and promote drugs escaping from the endolysosome is highly desired for overcoming MDR in chemotherapeutic treatment. This work reports a peptide-functionalized mesoporous silica nanomedicine (MSN) for encapsulation of curcumin (CUR) and doxorubicin (DOX) to significantly reverse the MDR in consequence of rapid endolysosomal escape and the inhibition of P-gp function. The nanomedicine is modified with the peptide of GFLGHHHRRGDS to encapsulate CUR and DOX with high loading efficiency and content. It can be selectively uptaken by the DOX resistant MCF-7/ADR cells via the specific recognition between RGDS and αvβ3 integrin to enter into lysosomes. Afterwards, the GFLG is cleaved by the lysosomal cathepsin B to release CUR and DOX from MSN. Furthermore, the imidazole-rich HHHR peptide can induce effective lysosomal membrane permeability which is beneficial for drug escape from lysosome, CUR-mediated inhibition of P-gp, and nuclear import of DOX in MCF-7/ADR cells. Confocal fluorescence imaging, flow cytometry and MTT assays demonstrate that the combination of CUR and the peptide-functionalized MSN markedly enhances the intracellular DOX concentration and achieves a successful chemotherapeutic treatment to MCF-7 or MCF-7/ADR cells, thus this work offers an effective strategy to overcome MDR.
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2016
·Vol. 11
·期 2
·e0149032
·
IF 3.057
Zhao Gao,Yu Han,Yunhui Hu,Xiaoyan Wu,Yongbin Wang,Xiaoqun Zhang,Jinjuan Fu,Xue Zou,Jun Zhang,Xiongwen Chen,Pedro A. Jose,Xi Lu,Chunyu Zeng
Both oxidative stress and inflammation are involved in the pathogenesis of contrast-induced nephropathy (CIN). Epigallocatechin-3-gallate (EGCG), a purified catechin from green tea, has antioxidant and anti-inflammatory effects. However, it is unknown whether or not EGCG is effective in treating CIN. Our present study found that intravenous administration of EGCG, either before or just after the establishment of CIN, had a protective effect, determined by normalization of serum creatinine and blood urea nitrogen levels, improvement in renal histopathological scoring and alleviation of apoptosis, accompanied by decreased oxidative stress and inflammation. Because EGCG is a potent inducer of the antioxidant heme oxygenase-1 (HO-1), we studied HO-1 signaling in CIN. HO-1 levels were increased in CIN; treatment with EGCG further increased HO-1 levels, accompanied by an increase in Nrf2, a regulator of antioxidant proteins. Interestingly, blockade of HO-1 with protoporphyrin IX zinc(II) (ZnPP) prevented the protective effect of EGCG on CIN. ZnPP also blocked the ability of EGCG to increase the activity of an antioxidant (superoxide dismutase), and decrease markers of oxidative stress (myeloperoxidase and malondialdehyde) and inflammation (myeloperoxidase and IL-1β), indicating that HO-1 is the upstream molecule that regulates the EGCG-mediated protection. To determine further the role of HO-1 on the EGCG-mediated inhibition of inflammation, we studied the effect of EGCG on the NLRP3 inflammasome, an upstream signaling of IL-1β. EGCG down-regulated NLRP3 expression, which was blocked by ZnPP, indicating that HO-1 links EGCG with NLRP3. Therefore, EGCG, via up-regulation of HO-1, protects against CIN by amelioration of oxidative stress and inflammation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Xinfeng Cheng,Yong Jin,Tongbing Sun,Rui Qi,Hanping Li,Wuhou Fan
A novel pH and oxidation dual-responsive and injectable supramolecular hydrogel was developed, which was formed from multi-block copolymer poly(ether urethane) (PEU) and α-cyclodextrin (α-CD) inclusion complexes (ICs). The PEU copolymer was synthesized through a simple one-pot condensation polymerization of poly(ethylene glycol), di(1-hydroxyethylene) diselenide, dimethylolpropionic acid and 3-isocyanatomethyl-3,5,5-trimethylcyclohexyl isocyanate. In aqueous solution, the amphiphilic PEU copolymers could self-assemble into nanoparticles with dual pH and oxidation sensitivities, which can efficiently load and controllably release a hydrophobic drug indomethacin (IND). Then a dual-drug loaded supramolecular hydrogel was obtained by addition of α-CD and hydrophilic model drug (rhodamine B, RB) into the resulting IND-loaded PEU nanoparticle solution. The rheology studies showed that the supramolecular hydrogels with good injectability underwent a pH-induced reversible sol–gel transition and an oxidation-triggered degradation behavior. The in vitro drug release results demonstrated that the hydrogels showed dual drug release behavior and the release rates could be significantly accelerated by addition of an oxidizing agent (H 2 O 2 ) or increasing the environmental pH. Therefore, this injectable and dual stimuli-responsive supramolecular hydrogel based codelivery systems could potentially be a promising candidate for controlled drug delivery systems.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER
·
2016
·
IF 3.586
Xinfeng Cheng,Yong Jin,Rui Qi,Wuhou Fan,Hanping Li,Xiaopeng Sun,Shuangquan Lai
A new diselenide crosslinked polyurethane nanogel was developed, which could respond to pH change and oxidaiton condition. Firstly, PEGylated polyurethane (MPEG-PUBM-MPEG) containing tertiary amino and Br groups was prepared, which can assemble into micelles in aqueous solution. Then the nanogels with diselenides crosslinked polyurethane (PU) core and hydrophilic PEG shell were prepared using sodium diselenide (Na 2 Se 2 ) as a crosslinking agent to react with the Br groups in the PUBM block. The resultant nanogels exhibited enhanced colloidal stability relative to the non-crosslinked micelles, which have a spherical morphology with a diameter about 110–120 nm. Interestingly, the nanogels displayed a pH-induced swelling and oxidation-triggered dissociation behaviors, which can efficiently load indomethacin (IND) drugs and showed an inhibited premature release behavior as compared to the non-crosslinked micelles. An accelerated release behavior at pH 5.0 (54% in 24 h) and a burst release under oxidation condition (95% in 20 h) were observed for the nanogels, indicating their dual pH and oxidation-triggered release behaviors. Considering their enhanced stability, pH-induced swelling and oxidation-triggered degradation characteristics, the diselenide crosslinked nanogels can serve as a potential system for controlled drug delivery.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）