ANALYTICA CHIMICA ACTA
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2017
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IF 4.95
Yujia Wang,Shucheng Sun,Irfan Azhar,Qian Zhang,Qishu Qu,Li Yang
Core-shell mesoporous silica (CSMS) microspheres with tunable mesopores in the shell are highly desired in various bioapplications. With novel CSMS microspheres that are synthesized using a convenient two-phase process, we report in this study the analysis of low molecular-weight (MW < 30 kDa) proteins by combining size-exclusion separation and enzyme immobilization . The obtained CSMS microspheres possess uniform diameter (1.3 μm with a shell thickness of 57 nm), large and tunable perpendicular mesopores (7.9 nm), high surface area (55.5 m 2 /g), large pore volume (0.12 cm 3 /g) and excellent water dispersibility. The CSMS microsphere-based enzyme nanoreactors have been fabricated by immobilizing trypsin on the pore channels of the CSMS microspheres using either physical absorption or covalent binding via thiol or aldehyde group with a high loading capacity of 11.8–6.1 mg/g. Due to the unique fibrous pore structure , low MW proteins can enter the channels in the shell to interact with immobilized trypsin, followed by analysis of the digestion products using MALDI-TOF MS or electrophoresis (CE) techniques. The properties and analytical performance of different trypsin-immobilized CSMS microspheres has been systematically evaluated. The results show that the peptide-sequence coverage of the smaller protein is enhanced by using trypsin-CSMS microspheres, indicating the size-dependent digestion which results from the size-exclusion interaction of the mesopores against the high-MW proteins. The present study would pave the way for further applications of mesoporous materials in proteome analysis.
研究领域:
生物技术
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分析化学
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材料科学
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 18
·4384
·
IF 4.2
Fangmin Wang,Xue Dong,Yuzhen Zhao,Zemin He,Wenqi Song,Chunsheng Li,Jiayin Li,Jianfeng Huang,Zongcheng Miao
The utilization of sheet structure composites as a viable conductive filler has been implemented in polymer-based electromagnetic shielding materials. However, the development of an innovative sheet structure to enhance electromagnetic shielding performance remains a significant challenge. Herein, we propose a novel design incorporating silver-modified nanosheet self-assembled hollow spheres to optimize their performance. The unique microporous structure of the hollow composite, combined with the self-assembled surface nanosheets, facilitates multiple reflections of electromagnetic waves, thereby enhancing the dissipation of electromagnetic energy. The contribution of absorbing and reflecting electromagnetic waves in hollow nanostructures could be attributed to both the inner and outer surfaces. When multiple reflection attenuation is implemented, the self-assembled stack structure of nanosheets outside the composite material significantly enhances the occurrence of multiple reflections, thereby effectively improving its shielding performance. The structure also facilitates multiple reflections of incoming electromagnetic waves at the internal and external interfaces of the material, thereby enhancing the shielding efficiency. Simultaneously, the incorporation of silver particles can enhance conductivity and further augment the shielding properties. Finally, the optimized Ag/NiSi-Ni nanocomposites can demonstrate superior initial permeability (2.1 × 10−6H m−1), saturation magnetization (13.2 emu g−1), and conductivity (1.2 × 10−3Ω•m). This work could offer insights for structural design of conductive fillers with improved electromagnetic shielding performance.
研究领域:
材料科学
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纳米技术
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DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
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2025
·
IF 14.2
Junfei Li,Yajie Xie,Jianming Zhao,Feilong Zhao,Abudureheman Bahatibieke,Guodong Liu,Haoye Meng,Yudong Zheng
Physiologically related electrical microenvironments play an indispensable role in nerve regeneration and repair. Simulating the electrophysiological characteristics of nerves through bionic construction of bioelectric fields has attracted wide attention, as it can regulate the behavior of nerve cells and promote the repair of nerve injuries. Electret is a kind of material that can maintain permanent polarization state without external electric field, and can be used as a new self-powered electrical stimulation method for nerve regeneration and repair. In this work, a novel self-powered conductive nerve conduit with both electret electrical effects and conduction activity was constructed to promote peripheral bioelectret nerve regeneration and repair. The microsphere electret is embedded on the bacterial cellulose structure through the self-polymerization reaction of dopamine, wherein the microsphere electret can provide micro-power and release electrical signals through the conducting layer of polydopamine. The maximum conductivity of the composite bioelectret can reach 6.2 × 10 −3 S/cm, and the output charge can reach 264 pC at a bending strain of 50 %. In vitro, composite bioelectret provided a good electrophysiological microenvironment for cells and enhance their adhesion, proliferation and migration capabilities. In the repair of sciatic nerve in rats, the electret effect and conductivity of composite bioelectret provide continuous electrical stimulation for repairing microenvironment, strengthen the communication among cells, and promote axon growth and myelin regeneration. Conductive composite bioelectret provides a new non-contact electrical stimulation strategy for nerve repair by combining the dual synergistic effects of conductivity and electret self-powered.
研究领域:
生物医学工程
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神经科学
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材料科学
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产品关联
DOI
高影响因子
POLYMER
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2024
·
IF 4.1
Fang Wang,Naipin Chen,Jiaqi Liu,Xinbao Zhu
The ability of an active protective organic coating to restore its protection functionality in the event of a coating defect is crucial for ensuring durable performance under harsh corrosive conditions. In the present work, a novel thermogenesis effect polyurethane complex microsphere with a particle size of approximately 430 nm was developed using CNCs and LNPs as Pickering emulsifiers. The obtained microspheres (4 wt%) were incorporated into epoxy-amine networks, resulting in improved toughness and bending strength. Additionally, tensile measurement results demonstrated that the composite resin exhibited a self-healing efficiency of 81.25 % at room temperature within 30 min. Meanwhile, for the NIR-triggered self-healing process, the damaged samples could be locally repaired quickly. Experiments also showed that the microspheres exhibited excellent anti-ultraviolet aging performance and satisfying storage stability for more than 20 days at room temperature. Importantly, the as-prepared epoxy complex resin served as an in-situ self-healing metal coating, confirming its long-lasting corrosion resistance. This work provides an efficient strategy for high-performance self-healing materials, particularly epoxy anti-corrosion coatings.
研究领域:
材料科学
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化学工程
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腐蚀与防护
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产品关联
DOI
高影响因子
International Journal of Minerals Metallurgy and Materials
·
2025
·Vol. 32
·期 3
·718-727
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IF 7.2
Wen Haoliang,Zhang Weidong
The increase in the utilization of infrared heat detection technology in military applications necessitates research on composites with improved thermal transmission performance and microwave absorption capabilities. This study satisfactorily fabricated a series of MoS 2 /BN- xyz composites (which were characterized by the weight ratio of MoS 2 to BN, denoted by xy : z ) through chemical vapor deposition, which resulted in their improved thermal stability and thermal transmission performance. The results show that the remaining mass of MoS 2 /BN-101 was as high as 69.25wt% at 800°C under air atmosphere, and a temperature difference of 31.7°C was maintained between the surface temperature and the heating source at a heating temperature of 200°C. Furthermore, MoS 2 /BN-301 exhibited an impressive minimum reflection loss value of −32.21 dB at 4.0 mm and a wide effective attenuation bandwidth ranging from 9.32 to 18.00 GHz (8.68 GHz). Therefore, these simplified synthesized MoS 2 /BN- xyz composites demonstrate great potential as highly efficient contenders for the enhancement of microwave absorption performance and thermal conductance.
研究领域:
材料科学
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物理化学
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产品关联
DOI
高影响因子
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
·
2024
·
IF 4.2
Rui Li,Zewei Wu,Yiyun Yang,Sijia Sun,Ruixin Ma,Hao Ding
A silica-loaded g-C 3 N 4 composite photocatalyst (M-SiO 2 /g-C 3 N 4 ) was prepared by grinding amorphous SiO 2 microspheres (M − SiO 2 ) with melamine and calcining the resulting grinding products. This composite was used for photocatalytic persulfate (PMS) activation to degrade carbamazepine (CBZ). The results showed that the degradation efficiencies of CBZ by M-SiO 2 /g-C 3 N 4 -2.5 (39.16 % of g-C 3 N 4 )-activated PMS were 78.92 % and 100 % after simulated sunlight irradiation for 60 and 120 min, respectively. These values were significantly greater than those of the system with pure g-C 3 N 4 (29.15 % and 42.4 %). In particular, the degradation efficiency of CBZ with M-SiO 2 /g-C 3 N 4 -2.5 decreased by only 9.47 % after 4 cycles of recycling. In the catalytic process, h + , SO 4 •− and O 2 •− were the main reactive species contributing to CBZ degradation. Compared with g-C 3 N 4 , loading g-C 3 N 4 on the surface of M − SiO 2 resulted in a decrease in g-C 3 N 4 lamellar stacking, which increased the number of active surface sites and improved the separation and migration of photogenerated carriers, improving the degradation performance.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
Polymers
·
2024
·Vol. 16
·期 5
·647
·
IF 4.7
Lin Qi,Teng Hang,Weijie Jiang,Sinong Li,Hui Zhang,Xiang Liang,Le Lei,Qiangqiang Bi,Hang Jiang,Yunxing Li
Microalgae are highly regarded as ideal materials for the creation of liquid biofuels and have substantial potential for growth and utilization. However, traditional storage and culture methods for microalgae are plagued by challenges such as uncontrolled growth, bacterial contamination, and self-shading among algae. These issues severely impede the photosynthetic process and the efficient extraction of biomass energy. This study tackles these problems by utilizing magnetic hydrophobic protein particles to stabilize water-in-oil Pickering emulsions. This allows for the micro-compartment storage and magnetic transfer of algae. Additionally, the successful encapsulation of Chlorella cells in high-internal-phase water-in-oil Pickering emulsions effectively mitigates the settling problem of Chlorella cells in the liquid phase, thereby enabling the potential use of Pickering emulsions for the confined cultivation of microalgae.
研究领域:
生物技术
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Qingjun Xu,Yang Zhao,Pingping Yuan,Xuan Ma,Sheng Wang,Lin Li,Ping Cheng,Shaoqi Qu
The escalating prevalence of multidrug-resistant (MDR) bacterial infections has emerged as a critical global health crisis, undermining the efficacy of conventional antibiotic therapies. This pressing challenge necessitates the development of innovative strategies to combat MDR pathogens. Advances in multifunctional drug delivery systems offer promising solutions to reduce or eradicate MDR bacteria. Inspired by the fact that the growth of bacteria requires essential nutrients, core–shell porous poly(lactic-co-glycolic acid) (PLGA) microspheres coated with pH-responsive polydopamine (PDA) were fabricated to improve delivery, resulting in enhanced efficacy through nutrient restriction and combination therapy. The PDA chelates iron ions in the environment, preventing bacteria from absorbing iron and thus suppressing their growth and proliferation. Subsequently, the released antibiotics from the porous PLGA core, rifampicin and polymyxin B, accelerate bacterial eradication by disrupting their inner and outer membrane structures. Such a multifunctional microsphere platform clears 99% Salmonella Typhimurium in 4 h and shows increased efficiency in a lethal intestinal infection model in mice. These findings provide a drug delivery system that integrates bacterial nutrient restriction and antibiotic killing, highlighting the potential of targeting bacterial iron regulation as a strategy for developing new antimicrobial delivery systems to address MDR bacterial infections.
研究领域:
生物医学工程
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微生物学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2024
·
IF 3.6
Qingqing Liu,Aihua Qu,Chuanlai Xu,Xinxin Xu,Liqiang Liu,Hua Kuang
In this research, we used a novel hapten to generate a sensitive monoclonal antibody (mAb) 2C3 that targeted etomidate (ET) and metomidate (MT). We used this mAb to establish a lateral-flow immunoassay (LFIA) that incorporated fluorescent microsphere sensors, enabling both the qualitative and quantitative detection of ET and MT within 10 min. Analysis indicated that the visual colorimetric values for ET and MT in water samples were 0.3 µg/kg, respectively, with quantitative detection ranges of 0.08 to 1.31 µg/kg and 0.08 to 2.21 µg/kg, respectively. The visual colorimetric values for ET and MT in urine samples were 0.3 to 1 µg/kg, with quantitative detection ranges of 0.12 to 3.13 µg/kg and 0.09 to 2.98, respectively. The visual colorimetric values for ET and MT in serum samples were 3 µg/kg, with quantitative detection ranges of 0.15 to 21.2 µg/kg and 0.08 to 13.8 µg/kg, respectively. Recovery tests and detection were conducted in water, urine and serum samples, validating the reliability of this method in real clinical samples. Collectively, our novel LFIA provides a promising option for rapid on-site detection of ET and MT.
研究领域:
医学检测
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免疫学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Electronic Materials
·
2024
·
IF 4.3
Chen Wang,Jianye Guang,Ruizhi Fan,Ming Lin,Jianuo Nie,Fang Wang,Wei Peng,Mengdi Lu
Flexible strain sensors have gained prominence in applications such as wearable devices, smart healthcare, and human motion monitoring due to their excellent adaptability and sensitivity. However, challenges such as high production costs and complex fabrication processes hinder their widespread commercial adoption. This study presented a fatigue-resistant polystyrene (PS) microsphere nanoarray optical strain sensor based on a polydimethylsiloxane flexible substrate, fabricated using a simple hemispherical indentation-assisted method. The sensor exhibited exceptional stability, enduring over 950 cycles, and demonstrated remarkable durability and sensitivity (−0.371 nm/%) at stretching speeds of 62.5 μm/s. Finite element analysis, supported by the experimental data, clarified the mechanical behavior of PS nanoarrays under varying strain angles and analyzed the influence of these angles on the sensing spectrum. Besides, fatigue points caused by repeated use could be naturally recovered, with the restored sensor maintaining excellent stability. The results suggested that this proposed flexible nanostructure offered a high-performance, cost-effective, and straightforward manufacturing option for strain optical sensors.
研究领域:
材料科学
·
传感器技术
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纳米技术
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产品关联
DOI
高影响因子
Small
·
2024
·
IF 13
Ya-Jun Fu,Rao-Kaijuan Wang,Cheng-Ye Ma,Li-Ya Wang,Si-Yu Long,Kai Li,Xing Zhao,Wei Yang
The delayed healing of infected wounds can be attributed to the increased production of reactive oxygen species (ROS) and consequent damages to vascellum and tissue, resulting in a hypoxic wound environment that further exacerbates inflammation. Current clinical treatments including hyperbaric oxygen therapy and antibiotic treatment fail to provide sustained oxygenation and drug-free resistance to infection. To propose a dynamic oxygen regulation strategy, this study develops a composite hydrogel with ROS-scavenging system and oxygen-releasing microspheres in the wound dressing. The hydrogel itself reduces cellular damage by removing ROS derived from immune cells. Simultaneously, the sustained release of oxygen from microspheres improves cell survival and migration in hypoxic environments, promoting angiogenesis and collagen regeneration. The combination of ROS scavenging and oxygenation enables the wound dressing to achieve drug-free anti-infection through activating immune modulation, inhibiting the secretion of pro-inflammatory cytokines interleukin-6, and promoting tissue regeneration in both acute and infected wounds of rat skins. Thus, the composite hydrogel dressing proposed in this work shows great potential for dynamic redox regulation of infected wounds and accelerates wound healing without drugs.
研究领域:
生物材料学
·
创伤修复
·
医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2025
·
IF 4.3
Lingchen Meng,Haodong Li,Bofan Liu,Ri Duan,Shenghua Yuan
The challenge of improving the wear resistance of Cu–Bi catalysts continues to persist in the industrial production of 1,4-butynediol (BYD) through the ethynylation of a formaldehyde reaction. Herein, We successfully prepared a hierarchical malachite microsphere catalyst by cooling the crystallization process under atmospheric pressure. The hierarchical malachite microspheres are uniform spheres with a diameter of 18 μm, which are composed of numerous two-dimensional microplatelets paralleling the sphere surface. The cooling rate is a crucial factor that significantly influences the development of the crystal morphologies. A slower cooling rate enhances the crystallinity of the catalyst particles, leading to the formation of larger microplatelets on the catalyst surface. Experimental results demonstrated that the spherical malachite catalyst, synthesized via the cooling crystallization method, exhibits excellent wear resistance and sustained catalytic performance in the ethynylation of formaldehyde for the synthesis of BYD.
研究领域:
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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