BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Ruixue Ma,Jun Cheng,Ying Liu,Dongwei Jia,Jiahao Wang
Given that excessive CO 2 induces reactive oxygen species (ROS) which inhibit photosynthesis and microalgal survival, this study explores how exogenous arginine enhances stress resilience and biomass yield of Nannochloropsis oceanica under high CO 2 stress. Arginine promoted NO synthesis, enhancing antioxidant enzyme activities to combat ROS damage. Transcriptomic analysis revealed that key enzymes in the Calvin cycle (PRK, GAPDH, FBPase and SBPase) and TCA cycle (CS, IDH, SCS and MDH) were significantly up-regulated, supporting carbon fixation and energy metabolism. Arginine also alleviated cell necrosis by extending the G1 phase, promoting DNA replication and protein synthesis, which increased the proportion of viable cells from 47 % to 84 %. Furthermore, the synthesis of chlorophyll a and carotenoid were enhanced, leading to 46 % increase in biomass yield. The results highlighted arginine as a promising metabolic regulator for enhancing stress tolerance and carbon fixation efficiency of N. oceanica under high CO 2 conditions.
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EMBO JOURNAL
·
2023
·Vol. 42
·期 6
·e112647
·
IF 11.4
Mingyue Xu,Ye Guo,Min Wang,Xing Luo,Xuning Shen,Zhimin Li,Lei Wang,Weixiang Guo
Neurogenesis in the developing and adult brain is intimately linked to remodeling of cellular metabolism. However, it is still unclear how distinct metabolic programs and energy sources govern neural stem cell (NSC) behavior and subsequent neuronal differentiation. Here, we found that adult mice lacking the mitochondrial urea metabolism enzyme, Arginase-II (Arg-II), exhibited NSC overactivation, thereby leading to accelerated NSC pool depletion and decreased hippocampal neurogenesis over time. Mechanistically, Arg-II deficiency resulted in elevated L-arginine levels and induction of a metabolic shift from glycolysis to oxidative phosphorylation (OXPHOS) caused by impaired attachment of hexokinase-I to mitochondria. Notably, selective inhibition of OXPHOS ameliorated NSC overactivation and restored abnormal neurogenesis in Arg-II deficient mice. Therefore, Arg-II-mediated intracellular L-arginine homeostasis directly influences the metabolic fitness of neural stem cells that is essential to maintain neurogenesis with age.
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ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Xiaoliang Zeng,Siqi Wei,Jing Hu,Lichen Gou,Li Wu,Xiandeng Hou
Arginine is considered as a biomarker of cystinuria and other diseases, and thus, it is of urgency to develop a simple and rapid method with high sensitivity and selectivity for arginine detection to meet the demand of on-site analysis and bedside diagnosis. In this work, a lanthanide metal–organic framework, La(TATB), was prepared using a triazine-based planar ligand, 4,4′,4″-s-triazine-2,4,6-triyltribenzoate (H3TATB), and lanthanide ion (La3+). La(TATB) can be used as a highly photosensitive agent to activate molecular oxygen to 1O2 to achieve efficient photosensitive oxidation of arginine accompanied by strong blue fluorescence emission under 302 nm UV irradiation. Due to the porous structure and high specific surface area of La(TATB), short-life 1O2 can effectively approach and react with amino acid substrate molecules, thus leading to higher sensitivity than other systems. Therefore, the “turn-on” fluorescence sensing of trace arginine can be realized, with a measured linear response range of 10–20,000 nM and a limit of detection as low as 7 nM. This method can be used for the detection of trace arginine in urine, which is conducive to the bedside diagnosis and rapid screening of cystinuria and other diseases. The proposed method not only expands the application scope of Ln-MOFs but also provides a new construction strategy for “turn-on” luminescence sensors.
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RSC Advances
·
2017
·Vol. 7
·期 66
·41561-41572
·
IF 3.108
Yuxian Wang,Ling Jiang,Qinke Shen,Jian Shen,Yuwang Han,Hongman Zhang
In the field of drug delivery, there is growing concern over the self-assembly of fatty acids, since their structures and properties are similar to liposomes, which are characteristically nontoxic for humans, possess good biocompatibility and biodegradation, and are without immunogenicity. Changes in pH, temperature, concentration, molecular structure of the amphiphilic molecule, head group type and hydrocarbon chain length have significant effects on the self-assembled structures; however, the degree of unsaturation can also influence the self-assembled structures of fatty acids. The aggregation behaviors of the mixtures of arginine and three kinds of unsaturated fatty acids (UFAs, oleic acid, linoleic acid and linolenic acid) in aqueous solutions have been investigated. Phase transition from transparent micelles (L1 phase) to birefringent bilayer structures (Lα phase) occurred with increasing amounts of UFAs. However, the Lα phase was different for macro and micro structures. Oleic acid with only one double bond was regarded as less flexible and curved, but with higher stacking, leading to a gradual phase transition process from the vesicle phase to the stacked lamellar phase in the Lα phase region, which could be proved by cryogenic transmission electron microscopy (cryo-TEM) and polarizing microscopy observations combined with 2H-nuclear magnetic resonance (2H NMR) and rheological properties. The turbid vesicle phase with weak viscoelastic properties were only observed in linoleic acid and linolenic acid systems because UFAs with more than one double bond are easier to bend, indicating that highly curved vesicles are likely to form. Through the analysis of the proposed mechanism and FT-IR spectra, the synergistic effects of non-covalent interactions, including hydrogen bonding, electrostatic interaction, and hydrophobicity, were considered to be responsible for the aggregation behaviors. Finally, vesicles displayed their potential in encapsulating water-soluble model drugs such as calcein. The results show that drug-loaded vesicles could play a role in the potential applications in drug sustained delivery systems.
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RSC Advances
·
2022
·Vol. 12
·期 15
·9223-9237
·
IF 4.036
Chen Cheng,Yi Wang,Yanling Lu,Shaojie Li,Hua Li,Jun Yan,Shiguo Du
In this work, ammonium polyphosphate (APP) was surface-modified by bio-based arginine (Arg) for the first time to enhance its flame retardance for fire-safety epoxy resin (EP). The structure of Arg modified APP (Arg-APP) was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), 1H nuclear magnetic resonance (1H-NMR), and scanning electron microscopy (SEM). The results illustrated that Arg was attached on the surface of APP through a cation exchange reaction. With Arg acting as the efficient carbon source, the char-forming ability of Arg-APP was significantly improved as illustrated by thermogravimetric analysis (TGA). The flame retardance of EP/APP and EP/Arg-APP composites was evaluated using the limit oxygen index (LOI), vertical burning tests (UL-94), and cone calorimeter tests (CCT). The results showed that at the same weight loading (15 wt%), Arg-APP had better flame retardance and smoke suppression performance compared with pristine APP, which can be attributed to Arg-APP constituting an integrated intumescent flame retardant (IFR) and facilitating formation of char residues with significantly expanded structures and higher carbonization degrees. When the weight loading of Arg-APP reached 25 wt%, the EP/Arg-APP composite could achieve an LOI value as high as 34.7%, pass V-0 requirements in UL-94 tests, and decrease the peak heat release rate and total smoke production by 83.5% and 61.1% compared with neat EP in CCT, respectively, indicating the superior flame retardance performance of Arg-APP. Finally, the effects of the flame retardant additives on the mechanical properties of EP were evaluated by the differential scanning calorimetry (DSC) tests and tensile-strain tests. At the same additive weight loading (15 wt%), the EP/Arg-APP composite showed higher glass-transition temperature and better tensile-strain properties compared with EP/APP composite, which can be attributed to the Arg shell structure improving the compatibility between APP and the organic substrate. In conclusion, this work presents a convenient and environmentally friendly method to improve the practical performance of APP.
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INSTRUMENTATION SCIENCE & TECHNOLOGY
·
2019
·
IF 1.118
X.-C. Fan,Q. Wang
A highly sensitive arginine sensor based on optical fiber surface plasmon resonance (SPR) and 11-mercaptoundecanoic acid (MUA) was studied and implemented in this work. The method of modifying the mercaptoundecanoic acid molecule on the surface of the sensing area was proposed initially by using molecular self-assembly technology in order to complete the detection of arginine. The detection principle is a hydrogen bond-based aggregation between mercaptoundecanoic acid and arginine. The close alignment of the mercaptoundecanoic acid molecules allows the aggregation of arginine to affect changes within the surrounding local refractive index. This aggregation was selective for the arginine molecules. The sensor probe has a detection limit of 0.005 μM and a sensitivity of up to 1474 nm/μM. The entire detection system, including the probe, has the advantages of being low cost, small in size, easy to operate, and uses low power. This approach has the potential to be developed as a mobile and fast-detection method.
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JOURNAL OF ANALYTICAL CHEMISTRY
·
2018
·Vol. 73
·期 8
·817-823
·
IF 0.971
Hu Q. Q.,Gao H.,Wang Y. M.,Ma W.,Sun D. M.
Abstract The poly(L-arginine)–graphene modified glassy carbon electrode ( GCE ) was fabricated and the electrochemical behavior of carmine and amaranth on this electrode was studied by cyclic voltammetric method. Compared with poly(L-arginine)/GCE and GCE, the poly(L-arginine)–graphene modified GCE improved the sensitivity for the determination of carmine and amaranth. The experimental results indicated that the oxidation peaks of carmine and amaranth were separated at 121 mV in phosphate buffer solution at pH 2.5. The linear range for the simultaneous determination of carmine and amaranth were 7.5 × 10 –7 –7.5 × 10 –5 M. The detection limits were 7 × 10 –8 M for carmine and 2.5 × 10 –7 M for amaranth. The method has been applied to the simultaneous determination of carmine and amaranth in drinks with satisfactory results.
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RUSSIAN JOURNAL OF ELECTROCHEMISTRY
·
2026
·Vol. 62
·期 1
·49-59
·
IF 0.9
Hong Sun,Yao Chuan,Zhang Wenxiao,Dang Jiayi,Zhao Chenguang,Wang Shicheng,Wang Junyan,Nie Jiwei,Wang Shengju
A poly L -arginine/β-cyclodextrin molecularly imprinted polymer-modified graphite electrode (PL-Arg/β-CD MIPs/GE) was developed for sensitive acetaminophen detection. The sensor achieved a wide linear range of 0.1–200 μmol L –1 and a low detection limit of 0.022 μmol L –1 , attributed to synergistic enrichment and electron transfer. It demonstrated excellent selectivity, reproducibility, and high recovery (98.20–102.80%) in real-sample analysis, showing great promise for practical drug monitoring applications.
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BMC Pregnancy and Childbirth
·
2026
·
IF 3.1
Li Ye,Li Yini,Wang Jiangzhu,Ma Liangkun,Zhang Suhan,Zhao Wei,Sun Yin
Objective To characterize longitudinal metabolic alterations associated with gestational diabetes mellitus (GDM) and to identify candidate metabolite signals for earlier risk assessment using a widely targeted metabolomics platform. Methods In this prospective cohort, 35 women who developed GDM and 35 matched healthy controls underwent fasting blood sampling in early pregnancy (6–13 weeks) and mid-pregnancy (24–28 weeks). Widely targeted metabolomics and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. Multivariate analyses, logistic regression, receiver operating characteristic (ROC) analysis, internal cross-validation, and restricted cubic spline modeling were applied within an exploratory framework. Results In early pregnancy, 11 differential metabolites were identified, including 6 amino acid-related metabolites, but no robust signal remained after false discovery rate (FDR) correction. By mid-pregnancy, 35 differential metabolites were identified, including 13 amino acid-related metabolites. Among 19 amino acid-related metabolites examined in focused analyses, L-arginine was the only amino acid-related metabolite that remained significant after FDR correction (FDR = 0.043). Higher mid-pregnancy L-arginine was associated with increased odds of GDM (aOR = 2.840, 95% CI 1.481–5.446), was positively correlated with 1-h ( r = 0.28) and 2-h ( r = 0.26) glucose levels during OGTT, and showed modest discrimination (AUC = 0.754; mean cross-validated AUC = 0.754). Quartile analyses showed a more pronounced risk increase in the highest exposure group, and restricted cubic spline analysis suggested an overall positive association with a possible nonlinear trend at higher levels. KEGG analyses highlighted arginine- and amino acid-related pathways, including arginine and proline metabolism and arginine biosynthesis, while network analysis suggested a potential link to mTOR signaling. Conclusion In this exploratory longitudinal analysis, pregnancies complicated by GDM showed progressively increasing amino acid-related metabolic disturbances from early to mid-pregnancy. Mid-pregnancy L-arginine emerged as a candidate metabolic signal associated with GDM risk, post-load glycemia, and greater risk elevation at higher levels. These findings support further investigation of amino acid-focused metabolic profiling as a research-stage approach for earlier GDM risk identification.
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 4
·1523-1537
·
IF 4.5
Zhang Dongqing,Yu Xiaoguang,Zhang Feng,Liu Wenqi,Miao Jiyuan,Li Xiaojin
High-temperature proton exchange membranes (HTPEMs) based on arginine-modified phosphoric acid-doped polybenzimidazole (Arginine-PBI-PA) and poly(tetrafluoroethylene) phosphoric acid-doped polybenzimidazole (PTFE-Arginine-PBI-PA) were fabricated and characterized for fuel cell applications. Arginine as filler was used to prepare HTPEMs for the first time, and the Arginine-PBI-PA membrane exhibited an excellent proton conducting ability with 24.1 mS cm −1 at 160 °C while maintaining mechanical strength. Furthermore, Arginine-PBI-PA composite membrane was also appropriate for membrane electrode assemblies (MEAs), and it exhibited better fuel cell performances (0.68 V@400 mA cm −2 ) compared with the PBI-PA membranes prepared. Molecular dynamics (MD) simulation revealed that the better electrochemical properties of Arginine-PBI-PA membranes over PBI-PA membranes were ascribed to the strong interactions between the basic sites and carboxyl groups of arginine and PA molecules, respectively. In addition, the higher proton conductivity for Arginine-PBI-PA membranes was attributed to the formation of a tighter H-bonding network and the interaction between arginine non-protonated oxygen (O) and PA protons (HPA). Graphical Abstract
Experimental investigation and molecular dynamics simulation of novel arginine/PBI composite membranes for high temperature PEM fuel cells
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DESALINATION
·
2022
·
IF 11.211
Jiawei Liu,Zhen Wang,Wenwen Li,Xuliang Wang,Yanlei Su
A noticeable improvement of nanofiltration (NF) membranes with high separation performance for desalination was reported in this work. Graphene quantum dots (GQDs) enhanced polyamide (PA) composite membranes (GPA) were composed of porous substrates and nanofilms The nanofilms as separation layers were fabricated by the support-free interfacial polymerization and possessed ultrathin thickness below 8.0 nm. GQDs-PA interface channels and GQDs-GQDs interface channels gradually generated and penetrated the PA functional layer, resulting in a dramatic permeance increase of GPA membranes. The GPA membranes were creatively modified with arginine (Arg) to improve hydrophilicity and narrow the water channels. The Arg-GPA NF membranes under optimized preparation exhibited exceedingly high permeance of 40.3 L m −2 h −1 bar −1 with Na 2 SO 4 rejection rate of 99.7 %, and 60.7 L m −2 h −1 bar −1 with Na 2 SO 4 rejection rate of 99.4 %. This synergistic strategy of GQDs regulation on PA nanofilm structure and Arg post-treatment modification may provide a reliable and efficient technique for the design of NF membranes.
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RSC Advances
·
2022
·Vol. 12
·期 5
·3157-3164
·
IF 4.036
Yi Xiao,Guofang Wang,Haomin Yi,Suhua Chen,Qinyu Wu,Siyi Zhang,Kexin Deng,Simeng Zhang,Zi-Qi Shi,Xiaoping Yang
Ru(bpy)3Cl2/TPrA is a prominent and widely used ECL system in analytical science. However, the co-reactant TPrA restricts the variety of applications because of its toxicity, volatility, and high cost. Here, we use arginine (Arg) as an alternative co-reactant for Ru(bpy)32+ by taking advantage of its low cost, non-toxicity, and biocompatibility. The mechanism of the Ru(bpy)32+/Arg system is that the deprotonated Arg can react with Ru(bpy)32+ to release emission. The similarity between the Ru(bpy)32+/Arg, Ru(bpy)32+/TPrA, and Ru(bpy)32+/DBAE systems demonstrates that Arg can be used as an alternative co-reactant for Ru(bpy)32+ ECL. As a proof of concept, we achieve an excellent performance for acetaminophen (Ace) detection based on the specificity of Arg and Ace, with excellent linearity, low detection limits, and good recoveries. This work is promising to expand the scope of the Ru(bpy)32+/Arg system and move forward their applications in bioassays.
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