RUSSIAN JOURNAL OF ELECTROCHEMISTRY
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2026
·Vol. 62
·期 1
·49-59
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IF 0.9
Hong Sun,Yao Chuan,Zhang Wenxiao,Dang Jiayi,Zhao Chenguang,Wang Shicheng,Wang Junyan,Nie Jiwei,Wang Shengju
A poly L -arginine/β-cyclodextrin molecularly imprinted polymer-modified graphite electrode (PL-Arg/β-CD MIPs/GE) was developed for sensitive acetaminophen detection. The sensor achieved a wide linear range of 0.1–200 μmol L –1 and a low detection limit of 0.022 μmol L –1 , attributed to synergistic enrichment and electron transfer. It demonstrated excellent selectivity, reproducibility, and high recovery (98.20–102.80%) in real-sample analysis, showing great promise for practical drug monitoring applications.
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Drug Design Development and Therapy
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2026
·
IF 6.1
Jiali Cai,Yaojian Zhang,Tian Zhang,Mengyi Wu,Dijun Wang,Chunyan Yin,Xueke Nie,Lan Chen,Zhihu Sun,Chanming Liu,Xiaojing Yan
Purpose: β-Amyrin acetate (BAA), a natural pentacyclic triterpenoid, exhibits greater lipophilicity than its antiepileptic precursor β-amyrin, implying enhanced blood-brain barrier (BBB) permeability. This study aimed to evaluate the antiepileptic efficacy of BAA and investigate its neuroprotective mechanisms, with a focus on calcium signaling.Patients and Methods: An epilepsy model was established in zebrafish using pentylenetetrazole (PTZ) to assess the effects of BAA on seizure-like behaviors, reactive oxygen species (ROS) levels, apoptosis, and inflammatory markers. Potential targets were predicted via network pharmacology and molecular docking. Furthermore, a glutamate (Glu)-induced HT-22 neuronal injury model was used to validate BAA’s effects on intracellular calcium homeostasis and downstream signaling pathways.Results: BAA significantly attenuated PTZ-induced seizure-like behaviors in zebrafish, specifically reducing the frequency of clonic and tonic-clonic seizures, as well as total movement distance and velocity. Concurrently, BAA mitigated oxidative stress, apoptosis, and neuroinflammation in the zebrafish. Network pharmacology and molecular docking analyses suggested the calcium signaling pathway as a potential target, with BAA showing high binding affinity to proteins such as Bcl-2 and JAK2. In vitro experiments suggested that BAA effectively alleviated Glu-induced calcium overload in HT-22 cells. It downregulated the Bax/Bcl-2 ratio, suppressed overactivation of the JAK2/STAT3 pathway, and consequently reduced neuronal apoptosis and inflammation. The BAPTA-AM co-treatment experiment further supported that the protective effect of BAA depends on the regulation of intracellular calcium homeostasis.Conclusion: BAA exerts antiepileptic effects by inhibiting neuronal calcium overload and modulating the JAK2/STAT3 signaling pathway, thereby attenuating neuroinflammation and apoptosis. These findings provide a solid experimental foundation for developing BAA as a promising antiepileptic candidate and elucidate its multi-target mechanism of action.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2026
·
IF 4
Songyu Wang,Junjie Luo,Kefan Ouyang,Genyuan Li,Fangjie Xiao,Qin Zhang,Ningxiang Yu,Qiang Zhao
BACKGROUND β -Carotene (BC) is highly susceptible to degradation during processing, storage and digestion. This study evaluated BC-loaded emulsions stabilized by succinylated rice glutelin (S-RG) against whey protein isolate (WPI) emulsions in terms of stability and bioaccessibility. RESLUTS S-RG emulsions exhibited significantly smaller particle sizes than WPI emulsions at pH 5 and 6, indicating better colloidal stability in mildly acidic conditions. Both systems showed comparable resistance to thermal treatment, ultrasonication and UV exposure. However, during in vitro gastrointestinal digestion, the S-RG emulsion demonstrated superior functionality, achieving a fat release rate of 18.33 ± 0.89%, a BC bioretention rate of 52.5 ± 0.72% and a bioaccessibility of 21.61 ± 1.33% – all notably higher than those of the WPI control. CONCLUSION S-RG effectively protects BC and enhances its bioaccessibility, performing comparably or better than animal-derived WPI. These findings support S-RG as a sustainable, plant-based alternative for delivering sensitive lipophilic nutrients in functional food emulsions. © 2026 Society of Chemical Industry.
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BIOTECHNOLOGY LETTERS
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2026
·Vol. 48
·期 2
·44
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IF 2.5
Ma Li,Wang Yuyu,Liu Ruiqi,Hu Jingjia,Zheng Xiaobing,Yue Xiaoyang,Liu Guanhua,He Ying,Zhou Liya
Developing stable and easy-to-operate biocatalysts is crucial for their use as industrial catalysts. Here immobilized whole-cell catalysts were used for β-alanine production by immobilizing recombinant Escherichia coli cells (containing hydroaminase) with diatomite. E. coli BL21 (DE3)/pET-30a ( +)-HAMase was genetically engineered for the efficient synthesis of β-alanine from acrylic acid and aqueous ammonia. Using glutaraldehyde as a cross-linking agent, polyethyleneimine (PEI) as a flocculant, and diatomite as the immobilization carrier, optimal immobilization was achieved with 8% (w/v) PEI solution, 5% (w/v) glutaraldehyde, and 100 mg wet cell/mL cell suspension, along with a PEI flocculation time of 2.5 h and glutaraldehyde cross-linking time of 1.5 h. The enzyme activity recovery rate reached 70.72%. Remarkably, the immobilized whole-cell catalysts exhibited excellent stability, retaining over 90% of initial enzyme activity after 12 h incubation at 45 °C and maintaining over 72% enzyme activity after storage for 60 days at 4 °C. Additionally, the immobilized cells demonstrated enhanced reusability, maintaining consistent β-alanine yield even after ten consecutive reaction batches with an average yield of approximately 80%.
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MOLECULAR BIOLOGY REPORTS
·
2026
·Vol. 53
·期 1
·440
·
IF 3.2
Ding Hongran,Slack Maya,McClure Holly,Gu Wen,Gu Helene,Kappes Ferdinand,Schultz Thomas F.,Somarelli Jason A.,Tsigkou Anastasia
Per- and polyfluoroalkyl substances (PFAS) are a class of synthetic chemicals extensively used as plastic additives. Their environmental persistence and potential for bioaccumulation have raised significant toxicological concerns. This study evaluates the cytotoxic and molecular effects of Perfluorooctanoic acid (PFOA) and its replacement compound, Perfluoro(2-methyl-3-oxohexanoic) acid (GenX), in human-derived skin (A375), liver (HepG2), kidney (SN12C), and colon (SW620) cell lines. The experimental design assessed cell viability, gene expression, and perturbations in key cellular stress pathways, with a particular focus on TGF-β/SMAD-mediated inflammation and the p53-driven DNA damage response. Our results demonstrate compound- and cell-type-specific toxicity, with GenX displaying reduced cytotoxicity compared to PFOA across all cell types. Molecular analyses revealed that both PFAS induced alterations in the TGF-β/SMAD pathway, consistent with a pro-inflammatory cellular state. Additionally, we observed activation of the DNA damage response, as evidenced by increased expression of ATM, ATR, and p53, alongside ribosomal stress-related changes in RPL5 and RPL11. Notably, while skin and liver cells exhibited similar response profiles, kidney and colon cells showed divergent modulation of SMAD signaling, suggesting tissue-specific susceptibility and mechanistic differences. These findings contribute to a deeper understanding of the differential toxicological profiles of legacy and replacement PFAS, with implications for health risk assessment and regulatory policy. Graphical abstract
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FASEB JOURNAL
·
2026
·Vol. 40
·期 6
·e71634
·
IF 4.3
Wenyu Gao,Hao Chen,Fangyu Lin,Jialin Liu,Tingxuan Huang,Han Wang,Xiaojiao Weng,Xinli Wang,Xiaoyan Lin,Tao Jiang
Gastric cancer (GC) is a leading cause of cancer‐related deaths and has high recurrence rate. Although fibronectin domain‐containing protein 1 (FNDC1) is implicated in GC progression, its molecular mechanisms remain unclear. Multi‐omics analyses (TCGA, GEO datasets) were used to assess FNDC1 expression and clinical correlation. In vitro (cell proliferation, invasion, EMT markers) and in vivo (xenograft) experiments, combined with molecular assays (Co‐IP, WB, ChIP), explored FNDC1's function and mechanism. FNDC1 was significantly upregulated in GC, correlating with advanced clinicopathological features and poor prognosis. Knockdown of FNDC1 suppressed GC cell proliferation, invasion, and metastasis by inhibiting EMT and Wnt/β‐catenin signaling. Mechanistically, FNDC1 competitively bound the WD5 domain (residues 224–254) of Gβ2, disrupting Gβγ–Dvl1 interaction. This prevented Dvl1 degradation, promoted Axin1 ubiquitination, and destabilized the β‐catenin–destruction complex (GSK3 β–APC–Axin1), leading to β‐catenin accumulation and Wnt pathway activation. FNDC1 drives GC malignancy by targeting the Gβ2–Dvl1 axis to activate Wnt/β‐catenin signaling, suggesting FNDC1 as a novel prognostic biomarker and therapeutic target. FNDC1 competitively binds Gβ2 to suppress the β‐catenin–destruction complex and promote the malignant behavior of gastric cancer (GC) by the following mechanism: elevated FNDC1 competes with Dvl for binding to Gβ2, thereby reducing Dvl–Gβ2 interaction; this reduction inhibits Dvl degradation in the cytoplasm; increased Dvl recruits Axin1, thereby disrupting the β‐catenin–destruction complex (GSK3β–APC–Axin) and blocking ubiquitination and degradation of β‐catenin; accumulated β‐catenin in the cytoplasm translocates to the nucleus, where it activates Wnt target genes and promotes epithelial–mesenchymal transition (EMT) in GC cells.
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EUROPEAN POLYMER JOURNAL
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2026
·
IF 6.8
Jiaen Chen,Kedi Yang,Yidan Wang,Jiaxing Yang,Qiang Lu,Xiaolu Mao,Li Ge
The separation of enantiomers is crucial in the pharmaceutical industry. Herein, a novel polymeric adsorbent was developed through the single-step cross-linking of [6-DHAA-6-deoxy]-β-CD, a dehydroabietylamine (DHAA) modified β-CD using epichlorohydrin. The resulting polymer demonstrates exceptional chiral recognition for the S-enantiomer of valsartan, a model chiral pharmaceutical, with a high adsorption capacity 20 mg·g −1 at 25 °C. Thermodynamics and kinetics studies reveal that S -valsartan adsorption onto poly-[6-DHAA-6-deoxy]- β -CD follows the Langmuir isotherm model and the pseudo-second-order kinetics, respectively. Molecular simulations suggest that chiral recognition arises from differing orientations and specific binding energies of the two enantiomers on the [6-DHAA-6-deoxy]-β-CD host. Under optimized conditions, racemic valsartan is successfully resolved with an excellent enantiomeric excess, leading to a high enantiopurity of 99.5% for the S -valsartan product. The current study not only presents a high–performance adsorbent for the enantioseparation of valsartan enantiomers, but more importantly, outlines a versatile pathway for developing sustainable separation media from natural, chiral resources.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
·
IF 6.7
Chi Lei,Qixiu Weng,Ping Song,Yingchun Zhu,Liangzhi Li,Lingzhao Kong,Lishi Yan
β-Glucosidase (BGL) is limited by the catalytic efficiency and environmental tolerance in the biomass biorefinery process. In this study, maleimide and its derivatives were used to modify BGL. FT-IR, circular dichroism, ζ-potential, ultraviolet, and fluorescence were used to characterize the conformational alteration of the thiol-modified enzyme and induced the enhancement of rigidity and substrate affinity. Molecular docking revealed that the modified BGL, especially Moc-Mal-BGL, had lower binding energy (−6.8 kcal mol–1) and shorter average hydrogen bond length (2.018 Å) with the substrate. The activity of Moc-Mal-BGL increased by 110.6% compared with native BGL and realized a 2.6-fold increase in catalytic efficiency kcat/Km (13.92 s–1/mM). The corresponding cellobiose conversion rate increased by 19.0%. Thermal stability, storage stability, solvent (GVL and DMSO) tolerance, and glucose tolerance of Moc-Mal-BGL were significantly improved. This work provides a rapid strategy to improve the catalytic efficiency and environmental tolerance of BGL and investigates the mechanism in depth.
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Communications Chemistry
·
2026
·
IF 6.9
Li Xiaopan,Shang Sensen,Liu Chenyu,Diao Jingjing,Li Xiang,Lv Xiaojun,Zhao Qingbo
Friedel-Crafts alkylation reactions are generally accepted to proceed by an electrophilic aromatic substitution (S E Ar) mechanism. Here, we observed that MCM-22 exhibited a high activity in the alkylation of thiophene with styrene at temperatures near ambient, while β was almost inactive. Three species were identified in the adsorption of thiophene on HMCM-22 and Hβ: the hydrogen-bonded thiophene, the protonated thiophene, and the thiophene oligomers. Styrene was chemically adsorbed on the two zeolites through its vinyl group. The infrared studies of the consecutive adsorption of thiophene and styrene on MCM-22 and β reveal that the major difference between the thiophene species on the two zeolites was the disappearance of the protonated thiophene on MCM-22. These results indicate that the reaction over MCM-22 occurs by the attack of a protonated thiophene molecule on the side chain of styrene that chemically adsorbed on the zeolite but not the conventional S E Ar mechanism. The alternative text for this image may have been generated using AI.
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Nanoscale Horizons
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2026
·
IF 6.4
Liyan Wang,Kaijun Feng,Shuai Liu,Jiehui Tian,Xiangdong Guo,Lin Geng,Yuchuan Xiao,Debo Hu,Lixing Kang,Qing Dai
Twist-polaritonics provides precise control of light-matter states through the stacking of atomically smooth, anisotropic layers, but has been restricted to the van der Waals (vdW) crystals. Non-vdW crystals, despite their symmetry-broken dielectric responses ideal for exotic polaritons, are challenging to prepare as suitable flakes due to their rigid 3D bonding networks, thus limiting the implementation of deep-subwavelength twist-polaritonics. We established a non-vdW polaritonic platform using ultrathin, single-crystalline β-Ga2O3 nanoflakes synthesized by exploiting its anisotropic bonding hierarchy on the quasi-layered (100)B plane. These flakes exhibited deep-subwavelength polariton confinement beyond λ/20. Moreover, their atomic-scale flatness enabled the assembly of twisted bilayers, in which we observed a topological transition of the polariton dispersion from hyperbolic to elliptical, directly controlled by the twist angle. This work positions β-Ga2O3 as a high-performance nanophotonic platform beyond the vdW family, while proposing that anisotropic bonding hierarchy provides a general strategy to unlock non-vdW twist-polaritonic functionality in a wide range of bulk crystals.
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IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
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2026
·
IF 7
Chen Liu,Xue Zhou,Hanyu Bai,Yana Zhang,Yong Zhao,Xuegang Li
Beta-actin (ACTB), a highly conserved cytoskeletal protein, is frequently dysregulated in early-stage malignancies, making its sensitive and specific detection crucial for cancer screening. Here, we develop a reflective fiber-optic SPR biosensor functionalized with copper–glycine engineered WS₂ nanosheets for label-free ACTB detection. The Cu–glycine modification not only enables WS₂ surface amination for efficient antibody conjugation, but also introduces Cu²⁺-mediated near-infrared absorption to amplify the plasmonic resonance response. Combined with the intrinsically large surface area and high refractive index sensitivity of WS₂, this synergistic design markedly enhances both molecular binding and signal transduction. The resulting WS₂-NH₂-based sensor achieves a refractive index sensitivity of 1467.5 nm/RIU, representing a 1.67-fold enhancement over unmodified fiber probes. For ACTB detection, it delivers an ultralow detection limit down to 9.766 pM, a broadened linear range of 2.4–120 pM (5×expansion), and a biosensing sensitivity of 84.477 nm/nM (1.4× improvement). Furthermore, at 1 ng/mL, a rapid response time of 20 min is achieved, demonstrating applicability for trace-level biomarker screening. Integrating high sensitivity, broad dynamic range, amplified spectral response, and strong anti-interference capability, this plasmonic WS₂-NH₂-modified fiber platform enables highly selective, label-free ACTB quantification. These findings highlight its strong potential for early cancer diagnosis, biomarker monitoring, and precision medical applications.
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JOURNAL OF FOOD ENGINEERING
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2026
·
IF 5.8
Qiao Chen,Hai-Xia Xu,Ji-Guang Chen,Lin Dai,Bao-Geng Fu,Qing-Feng Zhang
HP-β-CD and M-β-CD exhibited stronger complexation capacity with quercetin (Que) than native β-CD. • The solid complexes of Que and β-CD derivatives were prepared by spray-drying. • The complexes significantly improved the dissolution and solubility, and suppressed the recrystallization of Que. • M-β-CD carrier exhibited better bioavailability enhancement effects on Que in rat than HP-β-CD. Quercetin (Que) is a common flavonol with diverse biological activities. Cyclodextrins (CD) were used to enhance the oral bioavailability of Que and the underlying mechanisms were explored anew. The complexation reactions between Que and β-CD, 2-hydroxypropyl-β-CD (HP-β-CD) and methyl-β-CD (M-β-CD) were characterized by phase-solubility analysis and UV-Vis spectroscopic titration. The results revealed that HP-β-CD and M-β-CD exhibited stronger complexation capacity than native β-CD. It was novelty discovered that the reversible complexation reaction effectively suppressed the recrystallization of supersaturated Que. The solid complexes of Que with HP-β-CD and M-β-CD were prepared by spray drying with a drug loading capacity of 4.67 ± 0.08%. The SEM, XRD and DSC analyses showed Que lost its crystal structure in the complexes, while FTIR revealed the interactions between Que and CDs. The complexes significantly improved the dissolution and solubility of Que. In addition to being dissolved, approximately 25% Que existed in nanoparticle form in simulated gastrointestinal fluids. M-β-CD effectively maintained the particles in nanoscale, whereas HP-β-CD failed. After oral administration, Que mainly exists in rat circulating blood as glucuronide metabolites. Pharmacokinetics revealed that, compared with free Que, M-β-CD and HP-β-CD complexes did not significantly increase the blood concentration of Que, but increased the AUC (0-t) of quercetin glucuronide metabolite (Que-Glu) by 7.52 times and 4.44 times, respectively. The primary novelty of this work is the finding that in addition to solubility improvement, the suppression of Que recrystallization through CD complexation is also an important mechanism for bioavailability enhancement. Download: Download high-res image (282KB) Download: Download full-size image
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