Journal of Extracellular Vesicles
·
2023
·Vol. 12
·期 9
·12356
·
IF 16
Chuqi Liu,Dexiang Liu,Shen Wang,Lu Gan,Xiangliang Yang,Cong Ma
Exosomes play crucial roles in local and distant cellular communication and are involved in various physiological and pathological processes. Tumour-derived exosomes are pivotal to tumorigenesis, but the precise mechanisms underlying their secretion remain elusive. In particular, the SNARE proteins that mediate the fusion of multivesicular bodies (MVBs) with the plasma membrane (PM) in tumour cells are subject to debate. In this study, we identified syntaxin-4, SNAP-23, and VAMP-7 as the SNAREs responsible for exosome secretion in MCF-7 breast cancer cells and found that a SNARE complex consisting of these SNAREs can drive membrane fusion in vitro. Deletion of any of these SNAREs in MCF-7 cells did not affect MVB biogenesis and transportation, indicating their specific involvement in MVB–PM fusion. In addition, syntaxin-4, SNAP-23, and VAMP-7 play equivalent roles in exosome secretion in both HeLa cervical cancer cells and A375 melanoma cells, suggesting their conserved function in exosome secretion. Furthermore, deletion of VAMP-7 in 4T1 mammary carcinoma cells efficiently inhibited exosome secretion and led to significant attenuation of tumour growth and lung metastasis in mouse models, implying that VAMP-7 may hold promise as a novel therapeutic target for breast cancer.
研究领域:
癌症生物学
·
细胞生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale
·
2013
·Vol. 5
·期 10
·4291-4301
·
IF 6.233
Daoshu Lin,Qiang Cheng,Qian Jiang,Yuanyu Huang,Zheng Yang,Shangcong Han,Yuning Zhao,Shutao Guo,Zicai Liang,Anjie Dong
A low cytotoxicity and high efficiency delivery system with the advantages of low cost and facile fabrication is needed for the application of small interfering RNA (siRNA) delivery both in vitro and in vivo. For these prerequisites, cationic polymer–mesoporous silica nanoparticles (ssCP-MSNs) were prepared by surface functionalized mesoporous silica nanoparticles with disulfide bond cross-linked poly(2-dimethylaminoethyl methacrylate) (PDMAEMA). In vitro and in vivo evaluations were performed. The synthesized ssCP-MSNs are 100–150 nm in diameter with a pore size of 10 nm and a positively charged surface with a high zeta potential of 27 mV. Consequently, the ssCP-MSNs showed an excellent binding capacity for siRNA, and an enhancement in the cell uptake and cytosolic availability of siRNA. Furthermore, the intracellular reducing cleavage of the disulfide bonds cross-linking the PDMAEMA segments led to intracellular cleavage of PDMAEMA from ssCP-MSNs, which facilitated the intracellular triggered release of siRNA. Therefore, promoted RNA interference was observed in HeLa-Luc cells, which was equal to that of Lipofectamine 2000. Significantly, compared to Lipofectamine 2000, the ssCP-MSNs were more biocompatible, with low cytotoxicity (even non-cytotoxicity) and promotion of cell proliferation to HeLa-Luc cells. The in vivo systemic distribution studies certified that ssCP-MSNs/siRNA could prolong the duration of siRNA in vivo, and that they accumulated in the adrenal gland, liver, lung, spleen, kidney, heart and thymus after intravenous injection. Encouragingly, with the ability to deliver siRNA to a tumor, ssCP-MSNs/siRNA showed a tumor suppression effect in the HeLa-Luc xenograft murine model after intravenous injection. Therefore, the ssCP-MSNs cationic polymer–mesoporous silica nanoparticles with low cytotoxicity are promising for siRNA delivery.
研究领域:
纳米技术
·
药学
·
分子生物学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2021
·
IF 2.991
Lichuang Zhang,Hao Xu,Ziyi Cheng,Yan Wei,Ruize Sun,Ziwei Liang,Yinchun Hu,Liqin Zhao,Xiaojie Lian,Xia Li,Di Huang
Surface modification of nanoparticles with cellular protein components is a new biomimetic modification strategy, which utilizes the inherent affinity between homologous cells to introduce the same surface molecules into nanoparticles to improve the targeting performance. In this study, oleic acid (OA)-coated Fe3O4 nanocubes were prepared by a high-temperature thermal decomposition method and modified by 3, 4-dihydroxyphenylpropionic acid (DHCA); then, HeLa cell membranes were introduced onto the surface of the nanocubes through mixed coextrusion to try to endow them with the targeting function of natural cells. The results show that the prepared Fe3O4 nanocubes have high monodispersity, excellent water solubility, and biocompatibility. Moreover, the Fe3O4 nanocubes encapsulated by cellular protein show an obvious core–shell structure and the specific targeting property to HeLa cells is improved significantly, which is expected to be used in clinical targeted diagnosis and treatment of cancer.
研究领域:
纳米技术
·
癌症研究
·
生物医学工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Tiantian Liu,Shuting Yi,Gang Liu,Xin Hao,Ting Du,Jialin Chen,Tao Meng,Ping Li,Yaolei Wang
A facile method was developed to produce porous alginate beads (PABs) with a controllable interconnected porous structure with aqueous two phase (ATPS) emulsions as template for 3D cell culture. ATPS emulsions, containing two biocompatible immiscible aqueous phases of cell/dextran (Dex) mixture and alginate (Alg)/polyethylene glycol (PEG) mixture and stabilized by mPEG-BSA particles, were introduced to form PABs. The pore size of PABs could be controlled by changing the emulsification frequency and the volume ratio between the ATPS emulsions and PEG-Alg solution. Moreover, cells could be directly encapsulated in the interconnected pores due to the excellent biocompatibility of ATPS. HeLa and human liver cancer cells encapsulated in the PABs present stronger cell activity (>95 %), proliferation, and enhanced functions compared with the cells encapsulated in general alginate beads (GABs). It is believed that the PABs is a promising microcarriers for 3D cell culture in vitro .
研究领域:
生物技术
·
组织工程
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Metallomics
·
2024
·
IF 2.9
Shi Jia,Xie Leilei,Gong Wenjun,Bai Hehe,Wang Wenming,Wang Ai,Cao Wei,Tong Hongbo,Wang Hongfei
Four Ru(II)-centered isomeric complexes [RuCl(5cqn)(Val)(NO)] (1-4) were synthesized with 5cqn (5-chloro-8-hydroxyquinoline) and chiral Val (Val = L- or D-valine) as co-ligand, and their structures were confirmed using the X-ray diffraction method. The cytotoxicity and photodynamic activity of the isomeric complexes and their human serum albumin (HSA) complex adducts were evaluated. Both the isomeric complexes and their HSA complex adducts significantly affected HeLa cell proliferation, with an IC50 value in the range of 0.3–0.5 μM. The photo-controlled release of NO in solution was confirmed using time-resolved FT-IR and EPR spectroscopy techniques. Furthermore, photoinduced NO release in living cells was observed using a selective fluorescent probe for NO. Moreover, the binding constants (Kb) of the complexes with HSA were calculated to be 0.17–1.98 × 104 M−1 and the average number of binding sites (n) was found to be close to 1, it can serve as a crucial carrier for delivering metal complexes. The crystal structure of the HSA complex adduct revealed that one [RuCl(H2O)(NO)(Val)]+ molecule binds to a pocket in domain I. This study provides insight into possible mechanism of metabolism and potential applications for nitrosylruthenium complexes.
研究领域:
无机化学
·
药物化学
·
癌症研究
·
生物物理化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Guohua Qi,Haijuan Li,Ying Zhang,Chuanping Li,Shuping Xu,Minmin Wang,Yongdong Jin
Cytochrome c (Cyt c) release and cellular pH change are two important mediators of apoptosis. Effective methods to regulate or monitor such two events are therefore highly desired for apoptosis research and cancer cell therapy. Herein, we exploited electrostimulation to regulate cellular Cyt c release and apoptosis process, and by designing and preparing a smart and efficient plasmonic nanorobot (with surface-modified Cyt c-specific aptamer and 4-mercaptobenzoic acid) that is capable of Cyt c capture and self-sensing, we achieved real-time SERS monitoring of dynamic Cyt c release and simultaneous cell acidification in apoptosis during electrostimulation. Distinctly different molecular stress responses in the two events for cancerous MCF-7 and HeLa cells and normal L929 cells were identified and revealed. The method and results are valuable and promising for apoptosis and Cyt c-mediated biology studies.
研究领域:
生物医学工程
·
纳米技术
·
癌症研究
·
细胞生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 5
·期 10
·7773-7780
·
IF 3.708
Junping Zhang,Fuyao Liu,Tao Li,Xiuxia He,Zhenxin Wang
Taking advantage of the unique optical properties of upconversion nanoparticles (NP) and the feasibly modified nature of SiO2, NaYF4:Yb3+, Er3+@SiO2 core@shell (UCNP@SiO2) NPs with excellent morphology have been synthesized to investigate the surface charge effect on the interactions of NPs with cells. The cellular uptake behaviors, cytotoxicities and intracellular metal ion release of the as-prepared NPs have been investigated in two cell lines (HeLa and HepG-2) by fluorescence microscopy, MTT assay and inductively coupled plasma mass spectrometry (ICP-MS), respectively. It is found that the UCNP@SiO2 NPs with positive surface charge have much higher cell internalization ability and cytotoxicity than those with negative surface charge. The experimental results also demonstrate that the cytotoxicity of UCNP@SiO2 NP is closely related to the increase of intracellular reactive oxygen species (ROS) and metal ion release of internalized NPs.
研究领域:
纳米技术
·
细胞生物学
·
材料科学
·
毒理学
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产品关联
DOI
高影响因子
CHINESE SCIENCE BULLETIN
·
2013
·Vol. 59
·期 2
·147-153
·
IF 1.319
Wang Wei,Yan Xiao Li,Zhan Lei,Leng Fei,Yang Xiao Xi,Huang Cheng Zhi
A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris(2,2′-bipyridyl)ruthenium(II)@SiO 2 NPs] were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)
2+ 3
) as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru(bpy)
2+ 3
@SiO 2 NPs possessed superior advantages of strong fluorescence, low toxicity, and easy surface modification for bioconjugation. Cell imaging experiments indicated that Apt-Ru(bpy)
2+ 3
@SiO 2 NPs had great tendency to human bone marrow neuroblastoma cells (SK-N-SH cells), since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.
研究领域:
纳米技术
·
生物医学工程
·
细胞生物学
·
分子生物学
·
荧光成像
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Guo Sun,Ren-Wei-Yang Zhang,Xu-Yang Chen,Yu-Hua Chen,Liang-Hua Zou,Jian Zhang,Ping-Gui Li,Kai Wang,Zhi-Gang Hu
H 2 S plays a crucial role in numerous physiological and pathological processes. In this project, a new fluorescent probe, SG-H 2 S , for the detection of H 2 S, was developed by introducing the recognition group 2,4-dinitrophenyl ether. The combination of rhodamine derivatives can produce both colorimetric reactions and fluorescence reactions. Compared with the current H 2 S probes, the main advantages of SG-H 2 S are its wide pH range (5–9), fast response (30 min), and high selectivity in competitive species (including biological mercaptan). The probe SG-H 2 S has low cytotoxicity and has been successfully applied to imaging in MCF-7 cells, HeLa cells, and BALB/c nude mice. We hope that SG-H 2 S will provide a vital method for the field of biology.
研究领域:
化学
·
生物化学
·
生物成像
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2010
·
IF 5.429
Zhuyuan Wang,Shenfei Zong,Jing Yang,Jin Li,Yiping Cui
A dual-mode imaging probe for targeting cancer cells has been fabricated based on mesoporous silica coated gold nanorods (MS-GNRs) for the first time. In this probe, fluorescence and surface enhanced Raman scattering (SERS) signals can be generated independently by using different excitation wavelengths. To investigate the targeting performance of the probe, folic acid (FA) is conjugated on the outer surfaces of MS-GNRs as a targeting ligand and HeLa cells were used as model cancer cells because they overexpress folate receptors (FRs). The endocytosis mechanism was verified by competing experiments with free FA through both fluorescence images and SERS mappings. Moreover, the cytotoxicity of the probe was remarkably reduced in comparison with the GNRs without the silica shell as proved by the results of MTT assay . Compared with traditional imaging probes, this new type of nanoprobe has great potential for multiplexed imaging in living cells, which can be easily realized by using fluorescence and SERS signals.
研究领域:
纳米技术
·
癌症研究
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2019
·
IF 3.926
Ya-Liang Zhang,Rong Yang,Lin-Ying Xia,Ruo-Jun Man,Yi-Chun Chu,Ai-Qin Jiang,Zhong-Chang Wang,Hai-Liang Zhu
Twenty-four 1,2-diarylbenzimidazole derivatives were designed, synthesized and biologically evaluated. It turned out that most of them were potential anticancer drugs. Among them, compound c24 showed the highest anti-tumor activity (GI 50 = 0.71–2.41 μM against HeLa, HepG2, A549 and MCF-7 cells), and low toxicity to normal cells (CC 50 > 100 μM against L02 cells). In the microtubule binding assay, c24 showed the most potent inhibition of microtubule polymerization (IC 50 = 8.47 μM). The binding ability of compound c24 to tubulin crystal was verified by molecular docking simulation experiment. Further studies on HepG2 and HeLa cells showed that compound c24 could cause mitotic arrest of tumor cells to G2/M phase then inducing apoptosis. To sum up, compound c24 is a promising microtubule assembly inhibitor.
研究领域:
药理学
·
癌症研究
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Weibing Wu,Sijie Li,Wen Deng,Shanshan Zhai,Youlu Chu,Xingyue Liu,Peng Li,Lei Zhang
Surface enhanced Raman scattering spectroscopy (SERS) technology has received increasing attention in the bioimaging field. Herein, based on the support of cellulose nanocrystal (CNC) with thiol groups, one-dimensional (1D) "hot spot" structure was constructed by the self-assembly of gold nanoparticles (AuNPs) via Au-S bonding. Silica shell was subsequently coated on the CNC loaded with AuNPs to fabricate core-shell SERS nanoprobe, which was further successfully applied for the SERS imaging of Hela cells. Owing to the presence of silica shell, the nanoprobe exhibited excellent water-dispersible stability in phosphate buffer salt solution (PBS). Moreover, the silica shell effectively protected both the "hot spot" structure and the adsorbed Raman reporter of 4-mercaptobenzoic acid from external interference, thereby improving the signal stability of SERS nanoprobe. In addition, the plasma coupling of electromagnetic field between AuNPs and silica significantly enhanced the Raman signal of "hot spots". The SERS nanoprobe possessed good biocompatibility even at relatively high concentrations, and successfully achieved bioimaging of Hela cell using the Raman signal at 1580 cm -1 channel.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
分析化学
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）