ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Wei Zhang,Yan Jiang,Fengna Xi
Accurate detection of placental growth factor (PlGF) is crucial for early diagnosis, risk assessment, and clinical management of preeclampsia. Herein, we present an electrochemiluminescence (ECL) aptasensor targeting PlGF with high sensitivity, in which Ni(OH) 2 –CeO 2 composite nanocatalyst was confined in the vertically-order nanochanels to facilitate the ECL of luminol-dissolved oxygen (DO). These Ni(OH) 2 –CeO 2 composite nanocatalyst exhibited good catalytic performance towards oxygen reduction during electrochemical cathodic scanning and could catalyze the electrochemical oxidation of luminol anions. Compared to catalysts directly electrodeposited on indium tin oxide (ITO) electrode, the nanocatalyst confined within the ultra-small VMSF nanochannels demonstrated stronger ECL enhancement effects and higher stability. Additionally, the composite of Ni(OH) 2 and CeO 2 exhibited remarkable synergistic catalytic performance. By immobilizing PlGF recognition aptamer (Apt) on the VMSF surface, an aptasensor was constructed for the highly sensitive analysis of PlGF. Upon the capture of target PlGF (Ag) by recognition aptamer, the formed Apt-Ag complex blocking the nanochannel causes the hindered diffusion of luminol, ultimately leading to a decreased ECL signal for the quantitation of PlGF. Convenient, and sensitive detection of PlGF was achieved by the developed ECL aptasensor with wide linear range (10 fg/mL ∼ 10 ng/mL) and low limit of detection (LOD 1.2 fg/mL). Additionally, analysis of real human serum was realized. The aptasensor demonstrated good selectivity, anti-interference, reproducibility, and long-term storage stability.
研究领域:
生物分析化学
·
生物传感器
·
纳米技术
·
电化学
·
临床诊断
·
母胎医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Shanshan Wang,Minmin Wang,Chuanping Li,Haijuan Li,Chunhua Ge,Xiangdong Zhang,Yongdong Jin
Due to their high catalytic effect on the electrochemiluminescence (ECL) of luminol, noble metal nanoparticles (NPs) have been widely used in luminol-based ECL immunosensors. However the aggregation of NPs often resulted in the decrease of ECL signal and affected the stability and durability of the sensor. In this study, a microflower-like structure made of ultrathin Co/Ni-based metal-organic frameworks (MOF) nanosheets were used as a platform for luminol-functionalized AgNPs to construct an immunosensor for tumor marker alpha-fetoprotein. The nanosheet and microflower-like assembly structure of the Co/Ni-MOF greatly improved the ECL performance of the luminol-AgNPs system, attributed to their large surface area, resistance to particle agglomeration and the high catalytic activity of the Co/Ni-MOF. The ultrathin Co/Ni-MOF was full of atomically dispersed cobalt ions and nickel ions, which can catalyze the ECL reaction of luminol and H 2 O 2 . The as-fabricated immunosensor has a good sensitivity with detection limit of 0.417 pg mL −1 (S/N=3), and shows satisfactory performance in practical applications.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Yunxia Hu,Chaomin Chen,Yuan Liu,Sui Wang,Zhiyong Guo,Yufang Hu
In the present work, a dual signals electrochemiluminescence (ECL) ratiometric strategy was designed based on the synergic effect of the catalysis effect and ECL resonance energy transfer . It was found that CdSe quantum dots (CdSe QDs) can catalyze the oxidation of luminol to promote the signal intensity of luminol at 0.45 V. At the same time, a stronger cathodic ECL peak at −0.75 V from CdSe QDs was observed, which could be attributed to the resonance energy transfer between luminol as a donor and CdSe QDs as an acceptor. Moreover both signals from two different potentials increased with the increase hydrogen peroxide concentration. On the basis of the above results, an enzyme-free ECL sensor was fabricated by immobilization of coating CdSe QDs on Au-graphite oxide (GO-Au) composites modified glassy carbon electrode (GCE), and luminol solution as probe solution, which was used to detect rapidly and sensitively hydrogen peroxide in the range of 0.5–500 μM with a detection limit of 0.5 μM based on the ration of two signals. The sensor exhibited good reproducibility and sensitivity, suggesting that the simple method will be promising in the detection of active oxygen in environmental samples.
研究领域:
分析化学
·
生物传感
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2019
·
IF 4.018
Wenjing Qi,Maoyu Zhao,Yuling Fu,Hongkun He,Xue Tian,Di Wu,Yan Zhang,Ping Ping Hu
Lanthanide coordination nanoparticles terbium(III) (Tb 3+ )-based fluorescent nanoparticles (luminol-Tb NPs) are synthesized via aggregation-induced fluorescence strategy. Fluorescence of luminol-Tb NPs is enhanced nearly 40 times compared with luminol, which is the effect of aggregation-induced fluorescence by Tb 3+ . Luminol-Tb NPs with high fluorescence is utilized in fluorescent “turn-off” detection of uric acid (UA) within 1 min from 0.1 to 50 μM with the limit of detection (LOD) of 28 nM. The fluorescence quenching effect may be ascribed to photoinduced electron transfer occurs from luminol-Tb NPs to UA. This fluorescent method exhibits high selectivity in UA detection towards metal ions, glucose, ascorbic acid , dopamine, creatinine, urea and cysteine. When it is also applied in detection of UA in serum samples, 5 μM, 10 μM and 30 μM UA are respectively added to serum samples and recoveries range from 97.5% to 101.3% in serum samples. It demonstrates the practical value of luminol-Tb NPs for UA detection in serum.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2016
·
IF 2.687
Haibo Liu,Li Qiao,Ning Gan,Saichai Lin,Yuting Cao,Futao Hu,Jiayu Wang,Yinji Chen
Electrochemical polymerization of luminol molecularly imprinted polymer on carboxyl graphene (MIP/CG) was developed as stir bar sorptive extraction (SBSE) coating for selective pre-concentration and specific recognition of bisphenol A (BPA), hexoestrol and diethylstilbestrol in milk samples. Luminol was employed as monomer and BPA as the template to prepare MIP under 0–0.6 V electro-polymerization. Carboxyl graphene was modified on pencil lead as the substrate to increase extraction capacity. The preparation and extraction conditions affecting the extraction efficiency were optimized. Under the optimized conditions, a good linearity of three estrogens was obtained in the range of 4–1000 ng mL −1 . The average recoveries at the three spiked levels of the three estrogens ranged from 83.4% to 96.3% with the relative standard deviations (RSD) ≤ 7.1%. The limits of detection were in the range of 0.36–1.09 ng mL −1 . The developed method with low cost, high selectivity and good reproducibility can be potentially applied for determining trace estrogens in complex food samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 181
·期 11
·1223-1230
·
IF 3.719
Wang Ke,Wei Xiuhua,Tu Yifeng
This paper reports on the synthesis of AuAg and PtAg alloy nanoclusters (NCs) and their enhancement effect on the electrochemiluminescence (ECL) of luminol. The conditions of synthesis were optimized, and the structure and properties of the NCs were characterized by X-ray diffraction, transmission electron microscopy, electrochemistry, and optical spectroscopy. The NCs are found to intensify (by up to 20 times) the ECL of luminol in solution of pH 8.5. This finding can largely extend the useful pH range of the ECL of luminol. The enhanced ECL is strongly affected by oxygen and hydrogen peroxide, and the mechanism of enhancement is attributed to the accelerated production of reactive oxygen species. The enhanced ECL is also affected by phenolic artificial estrogens, and this was used for their determination with detection limits as low as 700 pg L− 1 (with AuAg) and 1.6 ng L− 1 (with PtAg). The method was applied to the determination of such estrogens in egg samples using diethylstilbestrol as a reference substance.
The alloy nanoclusters (AuAg/PtAg) have been synthesized. They enhance the electrochemiluminescence of luminal for about 20 times in pH 8.5 solution. It extended the practicable pH range of ECL and can be employed for detecting the oxygen matters and other related trace compounds.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
LUMINESCENCE
·
2016
·Vol. 32
·期 3
·277-284
·
IF 1.452
Jianqiu Chen,Juan Shu,Jiao Chen,Zhiran Cao,An Xiao,Zhengyu Yan
S,N co-doped carbon quantum dots (N,S-CQDs) with super high quantum yield (79%) were prepared by the hydrothermal method and characterized by transmission electron microscopy, photoluminescence, UV–Vis spectroscopy and Fourier transformed infrared spectroscopy. N,S-CQDs can enhance the chemiluminescence intensity of a luminol–H 2 O 2 system. The possible mechanism of the luminol–H 2 O 2 –(N,S-CQDs) was illustrated by using chemiluminescence, photoluminescence and ultraviolet analysis. Ranitidine can quench the chemiluminescence intensity of a luminol–H 2 O 2 –N,S-CQDs system. So, a novel flow-injection chemiluminescence method was designed to determine ranitidine within a linear range of 0.5–50 μg ml −1 and a detection limit of 0.12 μg ml −1 . The method shows promising application prospects.
研究领域:
分析化学
·
药物分析
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2016
·
IF 2.693
Min Zhou,Ailian Wang,Cong Li,Xiaowei Luo,Yongjun Ma
A novel flow-injection chemiluminescence method (FI-CL) was established for the determination of methionine in this paper, based on its strong enhancement on CL intensity of the luminol-KIO 4 system catalyzed by thioglycolic acid-capped CdTe quantum dots in alkaline media. Under the optimized conditions, the relative CL intensity was in proportion to methionine concentration in the range from 1.0×10 − 8 to 1.0×10 −5 g mL −1 with a detection limit of 6.6×10 −9 g mL −1 (3σ). The relative standard deviation (RSD) of the CL intensity for 1.0×10 −6 g mL −1 standard methionine solution was 0.97% (n=11). The proposed method was successfully applied to determine methionine in commercial pharmaceutical formulations with recoveries between 98.0% and 101.9%. The possible CL mechanism was discussed as well.
研究领域:
分析化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2015
·
IF 3.545
Xiaoqian Yu,Yingying Sheng,Yanjun Zhao,Aiping Fan
Bromophenol red, known as chemical indicator, was found to act as a novel potent signal enhancer of the peroxidase-catalyzed luminol–H 2 O 2 chemiluminescent (CL) reaction. It was found interestingly that bovine serum albumin (BSA) played a role in the enhanced chemiluminescent reaction (ECR). The addition of 2.5 mg mL −1 BSA into bromophenol red-enhance CL system showed 36 times stronger CL signal than that without addition of BSA. Mechanism study showed that the luminophors in the ECR were still 3-aminophthalate ion in an excited state (3-APA*). In addition, singlet oxygen ( 1 O 2 ) and hydroxyl radical ( ∙ OH) played a role in the ECR. The possible mechanism was discussed in the present study. The effect of pH, reaction time, and concentration of bromophenol red, BSA, luminol, and H 2 O 2 on CL intensity of the peroxidase-catalyzed CL reaction was studied. The detection limit value (LOD) of HRP and streptavidin-modified HRP in the proposed ECR with bromophenol red and BSA was 0.20 ng mL −1 and 0.05 ng mL −1 , respectively. This novel luminol–H 2 O 2 –HRP–bromophenol red-BSA CL system was applied to the CL detection of sequence-specific DNA based on a magnetic separation process. As low as 0.4 fmol of target DNA could be sensitively detected using the proposed CL system without any amplification process. The obtained results demonstrate very promising perspectives for using bromophenol red and BSA to improve the sensitivity of CL detection of sequence-specific DNA. In addition, this novel ECR system can also be generalized for CL immunoassay , CL western blotting, and so on.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jing Lei,Xiaoqing Sun,Yan Jin,Chunli Xu,Baoxin Li
Fe and Co single-atom catalysts (SACs) have been widely explored in many fields, while Zn SACs are still in their infancy stage. Herein, we unexpectedly found that atomically dispersed Zn2+ on N-doped carbon material (Zn-N-C) exhibited high catalytic activity on luminol-H2O2 chemiluminescence (CL) reaction. The Zn-N-C SACs were readily prepared through simple pyrolyzation of the cheap precursors (dopamine and ZnCl2). The mechanism of Zn SAC-catalyzed CL reaction of luminol-H2O2 was investigated in detail. The activity of Zn SACs originated from the Zn-N sites in the Zn-N-C structure. The monoatomic dispersion makes Zn2+ catalytic performance change from no activity to high activity in luminol-H2O2 CL reaction. This study demonstrated the particularity of the monatomic metal catalyst over the conventional metal ion. This work provides the unprecedented perspective for design of new metal SACs in CL reaction.
研究领域:
化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 13
·3541-3550
·
IF 4.157
Li Zimu,Xi Yachao,Zhao Anqi,Jiang Jianming,Li Bing,Yang Xinming,He Jianbo,Li Fang
Chemiluminescence (CL) reagent luminol was loaded into the porous structure of cobalt-imidazole metal-organic framework (MOF) ZIF-67 to obtain luminol-functionalized ZIF-67 (luminol@ZIF-67) with CL property. The morphology, composition, CL property, and CL mechanism of luminol@ZIF-67 were carefully investigated. The obtained luminol@ZIF-67 exhibited strong, stable, and visible CL emission that reacted with H 2 O 2 , attributed to the strong catalytic effect of ZIF-67 combined with the shortened diffusion distance between luminol and the catalytic center. The CL intensity of luminol@ZIF-67 was more than 550 times higher than that of luminol. Catechol can effectively quench the CL emission of luminol@ZIF-67 that reacted with H 2 O 2 . Then, a simple paper-based CL imaging detection method was developed for the detection of catechol by using a smartphone as a portable detector. The linear calibration curve of the developed CL assay for catechol ranged from 5 to 100 mg/L with detection limit of 1.1 mg/L (S/N = 3δ). The strong CL emission of luminol@ZIF-67 combined with the effective quench ability of catechol guaranteed high sensitivity of the detection method. The practical application ability of the developed CL assay was tested by the determination of catechol in tea and tap water samples, resulting in acceptable results. This work provides an effective paper-based CL detection method for catechol and enriches the species of the chemiluminescent MOF material. Graphical abstract
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Cui Wang,Yuqing Wang,Junhua Liu,Feng Li,Panpan Gai
Classical luminol-based chemiluminescence (CL) is the process of emitting light enhanced by the addition of coreactant hydrogen peroxide (H2O2). To address the instability issue of H2O2 decomposition, herein, we proposed a nanozyme-based biofuel cell (BFC) ingeniously coupled with a luminol CL system via in situ generation of H2O2. Specifically, the gold nanoparticle (AuNP) nanozyme with glucose oxidase-like activity can act as the anodic enzyme of BFC to catalyze the oxidation of glucose to produce H2O2 and electrons. In this case, H2O2 as a coreactant enhanced the CL intensity and the cathode of the BFC obtained electrons to generate the open circuit voltage (EOCV) signals. As a result, a dual-signal biosensing platform was successfully constructed. Interestingly, the AuNPs-catalyzed system operates in an alkaline medium, which precisely meets the pH requirement for luminol luminescence. Such a BFC-CL system not only greatly lessens the effect of unstable exogenous H2O2 on the signal stability but also enhances the CL of luminol. Furthermore, both CL and EOCV signals present a positive correlation with the glucose concentration. Therefore, this novel BFC-CL system shows good performance for dual-signal biosensing, which would serve as a valuable guideline for the design and application of BFC-based self-powered or CL biosensors.
研究领域:
生物化学
·
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）