JOURNAL OF APPLIED POLYMER SCIENCE
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2026
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IF 3.1
Zebin Xu,Hui He,Ailong Lv,Taotao Wang
Crosslinking modification of polymer emulsions is an effective approach to suppress swelling and improve coating durability. In this study, an epoxy-functional silane oligomer crosslinker (PGOPTS) was synthesized via a silane hydrolysis–condensation route using γ-(2,3-epoxypropoxy)propyltrimethoxysilane (GOPTS) as the monomer. A styrene–acrylate latex (SAL) crosslinked by PGOPTS was used, and the process variables were optimized using response surface methodology. Under the optimal conditions of 80.8°C reaction temperature, 0.58 wt% PGOPTS content, and 6.84 mol% tetrabutylammonium bromide (TBAB), the modified latex film exhibited a 35.70% reduction in water absorption and a 13.4° increase in water contact angle, indicating improved water resistance. The modified latex was further applied to polymer cement waterproof coatings. Silanol self-condensation and reactions with cement hydration products promoted the formation of an organic–inorganic co-crosslinked network. The resulting coating exhibited a 30.61% reduction in water absorption, a 14.2° increase in contact angle, and enhanced durability. This work demonstrates a practical crosslinking strategy for durable polymer cement waterproof coatings.
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Plants-Basel
·
2026
·Vol. 15
·期 16
·2500
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IF 4.7
Su Yan,Yinghui Gu,Yifei Kong,Yudi Xiang,Bohan Yang,Xiaomeng Su,Li Sheng,Kai Song
Aronia melanocarpacontains abundant phenolic compounds, including procyanidins, flavonols, flavan-3-ols, and phenolic acids, but its pronounced astringency limits its broader use in food applications. Catechol and other oxygen-donor motifs in plant phenolics are known to associate with Fe3+and can support metal–phenolic assembly. In this study, ultrasound-assisted cellulase–pectinase extraction was used to recover anA. melanocarpapolyphenol extract, which was subsequently combined with Fe3+to construct a plant-derived hair dye coating on keratin fibers. Under optimized extraction conditions, the total phenolic yield reached 82.315 mg gallic acid equivalents (GAE)/g dry fruit. A limited targeted LC–MS/MS panel was used to evaluate 12 selected non-anthocyanin phenolic analytes, including procyanidin dimers, a flavan-3-ol, flavonols, and phenolic acids; the contribution of the pH-sensitive anthocyanin fraction was not resolved. Among the tested metal ions, Fe3+produced the strongest chromogenic response. The optimized L-cysteine pretreatment–Fe3+mordanting–polyphenol dyeing sequence produced a ΔE* of 55.42. Adsorption experiments empirically described the uptake behavior of the polyphenol extract, whereas complementary surface, spectroscopic, elemental, diffraction, thermal, and wettability analyses were consistent with the presence of a relatively uniform Fe-containing polyphenol coating at the hair fiber interface. Neither the adsorption-model fits nor the individual characterization techniques uniquely resolved the underlying molecular mechanism. Preliminary HET-CAM, single-exposure dermal, and acute eye irritation assessments showed no obvious acute irritation under the tested conditions. An exploratory image-based analysis examined whether standardized hair tress photographs could approximate a predefined colorimetric score. Because only 30 independent original samples were available and no external validation was performed, this analysis was treated solely as a proof of concept. This laboratory proof-of-concept extends established Fe3+–polyphenol assembly chemistry to a compositionally complexA. melanocarpaextract for hair fiber coloration. Further work is required to clarify anthocyanin behavior, simplify the sequential protocol, and benchmark its performance against representative commercial hair dyes.
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VACCINE
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2026
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IF 3.4
Xiaotong Wang,Chanyuan Dai,Xiang Ji,Haiyue Zheng,Hao Wu,You Yang
Pseudomonas aeruginosa is a critical-priority pathogen responsible for life-threatening infections in immunocompromised individuals, yet its extensive antigenic heterogeneity has impeded the development of immunotherapeutics. Alginate, a structurally conserved surface polysaccharide that forms the backbone of the biofilm matrix of mucoid P. aeruginosa strains in chronic infections, represents a compelling target. Here, we report the generation and functional evaluation of mAb 546B, a murine IgG1 monoclonal antibody directed against the synthetic mannuronic acid tetrasaccharide epitope 1 . This antibody bound to the surface alginate of both mucoid and nonmucoid P. aeruginosa strains and induced pronounced bacterial aggregation. Notably, 546B inhibited biofilm formation, disrupted established biofilms, and reduced the minimum inhibitory concentration of meropenem across multiple strains. In a bacterial clearance model, 546B monotherapy reduced lung bacterial burden compared to PBS controls, and its combination with meropenem achieved a marked reduction accompanied by significantly attenuated pulmonary inflammation and histopathological injury. In a lethal PAO1 pneumonia model, 546B alone improved the 7-day survival rate of mice, and combination with meropenem could further enhance the survival rate. These findings establish 546B as a promising monoclonal antibody that targets alginate-mediated biofilms, supporting its further development as a passive immunotherapeutic against P. aeruginosa infection.
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ACS Sustainable Chemistry & Engineering
·
2026
·Vol. 14
·期 34
·15218-15228
·
IF 7.6
Wu Mingxiong,Luo Shuai,Xu Heyi,Jin Jing,Tan Haiying,Jiang Wei
The increasing demands for environmental adaptability and recyclability in high-performance hot melt adhesives have driven the development of a novel material with ultra-high bonding strength. In this work, biodegradable poly(propylene carbonate) (PPC) and high-strength polyethylene terephthalate glycol (PETG) were linked via chain extension using methylene diphenyl diisocyanate to fabricate a PPC-PETG hot melt adhesive. The resulting adhesive exhibits exceptional adhesion across a wide range of substrates, achieving a maximum single-lap shear strength of 29.9 MPa on stainless steel. Notably, after five consecutive recycling cycles, the adhesive strength remains as high as 21.0 MPa. The PPC-PETG adhesive also demonstrates excellent resistance to both acidic and alkaline environments on stainless steel substrates, with single-lap shear strength significantly improved compared to neutral conditions. Its overall performance substantially surpasses that of conventional commercial adhesives and previously reported counterparts. In practical applications, this adhesive has been successfully used to fabricate high-performance plywood by bonding various hardwoods and softwoods, yielding flexural strengths of at least 150 MPa and flexural moduli of at least 14 GPa.
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ANALYTICAL CHEMISTRY
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2026
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IF 7.3
Boyu Wu,Jiawei Chen,Yanheng Huang,Pan Li,Qingmei Deng,Ronglu Dong,Liangbao Yang
Surface-enhanced Raman spectroscopy (SERS) offers exceptional sensitivity and specificity for biomolecular detection, particularly in analyzing serum tumor biomarkers. However, its application is hindered by spectral complexity and matrix interference. Although machine learning (ML) has shown promise in SERS data analysis, existing quantitative models for biomarker detection lack generalizability and interpretability. To address these challenges, we developed an interpretable stacked ensemble model (ISEM) that integrates feature selection, ensemble learning, and explainable artificial intelligence (XAI) for quantifying 12 serum tumor biomarkers. To ensure reliable quantitative analysis, we validated the acquired SERS data using uniform and reproducible Au nanoparticle substrates. Preprocessing, least absolute shrinkage, and selection operator (LASSO) feature selection were employed to establish a foundation for accurate quantification. Subsequently, 12 serum tumor biomarkers were quantified, demonstrating the superior performance of ISEM, which achieved a high predictive accuracy, with R2 values exceeding 0.9 for all biomarkers. Crucially, based on the quantitative results, we provided molecular-level interpretability for structure-spectrum correlations using Shapley additive explanations (SHAP), revealing how glycosylation reactions, matrix interference, and spectral overlap influence prediction accuracy. Furthermore, the capability of ISEM to quantify multiple biomarkers in unseen samples was confirmed through validation of its generalizability. Our study establishes an ensemble-driven, interpretable framework for quantitative biomarker analysis in a complex biological matrix, demonstrating significant potential for early cancer diagnosis and screening.
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BIOSENSORS & BIOELECTRONICS
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2026
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IF 11.8
Mengqing Cheng,Xinyue Chen,Hongrui Cheng,Xianglin Gao,Haoyue Ao,Xinyang Bao,Xiaojun Song,Yulei Tai,Dazhi Jin,Lei Zhang
Clostridioides difficile ( C. difficile ) infection (CDI) represents a formidable global healthcare challenge, necessitating the development of rapid, accurate, and cost-effective diagnostic platforms to mitigate nosocomial transmission and improve patient outcomes. Compared with the conventional methods, CRISPR/Cas systems featured by specific target reorganization by a single chain RNA, coupled with electrochemical technology enables highly sensitive detection of various biomarkers. However, their application to CDI has remained unexplored due to the lack of tailored crRNAs. Herein, we present the integration of CRISPR/Cas12a with electrochemical transduction for the direct detection of C. difficile . A novel crRNA was engineered to specifically recognize the toxin B gene ( tcdB ), activating the trans-cleavage activity of Cas12a upon target binding. This cascade triggers the cleavage of immobilized ssDNA reporters on the electrode surface, generating measurable amperometric signal changes. The developed biosensor demonstrates exceptional performance, achieving a detection limit of pM level for tcdB DNA within 40 min, while exhibiting high specificity against non-target pathogens and robust stability over 7 days. This work establishes a rapid and reliable CRISPR-electrochemical diagnostic platform, offering significant potential for point-of-care CDI management.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
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IF 5.9
Ke Wang,Tao Xu,Ruixiao Li,Chao Tao,Weiyi Chen,Shiying Mao,Meiling Zhou,Bin Liao,Jinlu Shang,Li Deng
Thermosensitive MGA@Gel enables prolonged intratumoral retention and controlled release. • GA-mediated HSP90 inhibition reduces thermotolerance and enhances PTT efficacy. • MGA@Gel exhibits stable photothermal performance with rapid heating. • MGA@Gel achieves effective tumor inhibition with good biocompatibility in 4T1 mice. Triple-negative breast cancer (TNBC) is highly aggressive and prone to recurrence, and durable therapeutic efficacy is often difficult to achieve with monotherapy. Photothermal therapy (PTT) offers advantages such as minimal invasiveness and spatiotemporal controllability and induces tumor cell death through localized hyperthermia. However, thermal stimulation simultaneously activates protective heat-stress responses in tumor cells, among which the inducible upregulation of heat shock protein 90 (HSP90) enhances thermotolerance and consequently compromises the efficacy of PTT. To overcome this limitation, we proposed a synergistic strategy based on HSP90 inhibition to induce tumor cell apoptosis while sensitizing tumors to PTT, and constructed an intratumorally injectable thermosensitive hydrogel for localized drug delivery. The HSP90 inhibitor geldanamycin (GA) was loaded into mesoporous polydopamine (MPDA) to form MGA, which was subsequently encapsulated within a PNIPAM/β-glucan composite thermosensitive hydrogel, yielding MGA@Gel. Both in vitro and in vivo studies demonstrated that MGA@Gel markedly enhanced antitumor efficacy under near-infrared (NIR) laser irradiation and effectively suppressed HSP90-associated heat-stress tolerance. Notably, in a 4T1 tumor-bearing mouse model, a single intratumoral injection established a sustained local drug depot. When combined with NIR laser irradiation, this system enabled efficient tumor suppression, pronounced downregulation of HSP90 signaling, increased apoptosis, and reduced tumor cell proliferation. These findings confirm that GA, by blocking the heat-stress protective pathway, effectively potentiates the antitumor effects of PTT. Overall, we developed a thermosensitive in situ hydrogel delivery system that enhances PTT via HSP90 inhibition, thereby overcoming tumor thermoresistance and providing a promising strategy for precise local treatment of TNBC.
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JOURNAL OF NEUROSCIENCE METHODS
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2026
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IF 2.5
Moran Zhang,Xin Zeng,Min Chen,Jinxuan Liu,Li Li,Hongxiang Chen,Fangyi Chen
Automated platform enables detailed and unbiased quantification of scratching behavior. • High-throughput processing increases efficiency and reduces manual effort. • Improved YOLOv8s balances efficiency and precision for fine-grained behavioral detection. • Immediate analytics support on-the-fly experimental monitoring and adjustments • Compact edge-computing design ensures scalable and easy deployment in laboratories Background Quantitative analysis of rodent scratching is essential for studying somatosensory and neuroimmune mechanisms, yet scalable and high-throughput analysis remains challenging. New method We developed RMScratcher, a compact platform combining multi-channel acquisition with Jetson TX2–based edge computing and an enhanced YOLOv8s framework. Incorporating GhostConv and Context Aggregation modules, the system achieves efficient and accurate detection of bouts, touch, duration and scratching region. Results RMScratcher achieved > 95% accuracy in distinguishing scratching from other behaviors, reliably quantified bout, touch and duration, and scratching region. Performance was robust across multiple rodent models and experimental conditions. Comparison with existing methods Manual scoring is slow and prone to bias, while existing automated tools often lack throughput and generalizability. Crucially, there is no known literature that identifies the scratching region. RMScratcher provides these capabilities with minimal hardware requirements. Conclusions RMScratcher extends the methodological toolkit for behavioral neuroscience by integrating video recording with intelligent computation. The platform facilitates reproducible studies of somatosensory function, neuroimmune interactions, behavioral phenotyping, and the neural mechanisms underlying abnormal sensory behaviors. Download: Download high-res image (169KB) Download: Download full-size image
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SENSORS AND ACTUATORS B-CHEMICAL
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2026
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IF 8.3
Xiuyun Li,Anle Ge,Zhidian Diao,Wenjie Zhao,Lingyan Kan,Sijun Luo,Wei Gao,Xixian Wang,Jian Xu,Bo Ma
Single mammalian cell heterogeneity plays a critical role in biological research. However, current microfluidic platforms for single-cell analysis still face the challenge of simultaneously achieving high-efficiency capture, cost-effective target cell sorting, and functional integration. To overcome these challenges, we developed a novel microfluidic platform for high-throughput single-cell analysis and sorting. This platform integrates multiple functions, including high-efficiency capture, online incubation, in situ detection, and target sorting. The chip employs a cost-effective polydimethylsiloxane-indium tin oxide (PDMS-ITO) glass configuration with arrayed capture microstructures, achieving average capture efficiency exceeding 93% for mammalian cells (RAW264.7, HCT116, and MCF7) across a size range of approximately 10–20 μm. The platform allows online incubation and artificial intelligence (AI)-assisted detection using both bright-field and fluorescent imaging. Cell viability rates consistently exceeded 80% after 24 h incubation and live/dead staining. A laser-induced microbubble strategy enabled precise releasing target single cells which were subsequently collected in a 96-well plate. In addition, the platform was applied to evaluate heterogeneity in reactive oxygen species (ROS) responses among macrophages, revealing intercellular differences under stimulation. This high-throughput, cost-effective platform combines high capture efficiency, advanced detection, and cell sorting functionality, demonstrating its utility for single mammalian cell heterogeneity studies and downstream applications.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
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IF 5.9
Weiqiang Wang,Xuan Wang,Dongdong Hu,Xiaoqing Song,Jiaying Qiu,Jiajia Gao,Yutong Liu,Yixuan Tao,Honghua Ge
The extracellular matrix (ECM) is a dynamic, hierarchically structured network essential for mechanical support and regulation of cell behavior. Mimicking its complexity in synthetic materials remains a major challenge. Here, we present a straightforward strategy to fabricate ECM-inspired, dynamically phase-separated hydrogels (DyPS-MAPgel) by methacrylation of mussel adhesive proteins (MAPs), followed by metal ion-induced phase separation and in situ photopolymerization. Leveraging the intrinsic ability of MAPs to undergo biomolecular phase separation and subsequent covalent crosslinking, the resulting hydrogels exhibit tunable mechanical properties, strong tissue adhesion, self-healing, antibacterial, and antioxidant activities. Importantly, DyPS-MAPgels support stem cell proliferation and lineage-specific differentiation both in vitro and in vivo through integrin-dependent mechanotransduction pathways. This work demonstrates the potential of phase separation–mediated assembly as a robust strategy for constructing ECM-mimetic materials and provides insights into ECM-cell interactions. Our findings offer a versatile platform for regenerative medicine applications, including bioadhesion, 3D bioprinting, and tissue engineering.
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SENSORS AND ACTUATORS B-CHEMICAL
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2026
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IF 8.3
Yuan Xu,Songbai Cao,Yuheng Liu,Jun Peng,Mingyi Yu,Youjian Liu,Yuhua Cai,Sebastien Balme,Tianji Ma,Zhong Cao
Concanavalin A (Con A) is a glycoprotein binding lectin which involves cytotoxicity, cell death and cancer diagnosis. An iontronic sensor was designed using nano-confined polysaccharide-protein complexation. Dextran was functionalized onto conical track-etched nanopore surface as a lectin probe. When tetramer Con A agglutinate with dextran inside the conical nanopores, the nanopore is effectively blocked by steric hindrance, enabling a molecularly gated response that results in an amplified decrease of ionic conductance. Subsequent elution removes Con A and reopens the channel, allowing the sensor to be regenerated and reused. In 1 mol/L KCl PBS buffer (pH 7.4), the sensor achieved an ultra-low detection limit of 0.19 ng/mL and exhibited a broad linear range from 10 ng/mL to 100 mg/mL, with channel conductivity showing clear logarithmic dependence on Con A concentration. The sensor exhibits excellent selectivity toward Con A and maintains stable performance for 20 days. With its label-free design, simple structure, reusability and successful application in real samples, this iontronic nanopore sensing platform offers a highly promising tool for lectin monitoring and related bioanalytical applications.
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Nature Communications
·
2026
·
IF 18.1
Xue Wang,Yuzhuo Wang,Yuxin Li,Yujiao Sun,Peiyuan Mao,Simiao Liu,Jieying Ou,Xuemei Wang,Fazheng Ren,Hao Zhang
Invasive and inconvenient blood tests hinder the diagnosis and treatment of micronutrient deficiencies, which affect billions worldwide. Wearable sweat sensors offer a noninvasive and real-time alternative. However, on-body monitoring of multiple low-abundance vitamins in sweat remains challenging. Here, we show a wearable electrochemical platform that utilizes bioreceptor-functionalized gold nanoflower/sulfur and nitrogen codoped carbon to detect six low-concentration vitamins in sweat (vitamins B 1 , B 2 , B 7 , B 9 , B 12 , and D) with nanomolar-level sensitivity. Integrated system incorporates iontophoretic sweat induction, microfluidic sampling and real-time vitamin detection with calibration. Human studies revealed temporal profiles of VB 9 levels in sweat in response to oral VB 9 supplementation and VB 9 -rich diets, and a strong sweat-serum correlation ( r = 0.849) has been found. The significant differences in VB 9 levels observed between smokers and nonsmokers demonstrated the potential of this technology for vitamin monitoring in diverse populations. Our platform facilitates early detection of nutritional imbalances and advances personalized nutrition. Nutrient deficiencies are widespread, but blood tests are often inconvenient. Here, the authors develop a wearable electrochemical platform for noninvasive, real-time detection of multiple low-concentration vitamins in sweat and validate its performance in human studies.
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