SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xin Liu,Shiyue Niu,Liqian Niu,Xian Wang,Xinxin Liu,Xin Guan,Xinyu Fan,Shuyun Bi
A highly sensitive surface-enhanced Raman spectroscopy (SERS) approach was established for the detection of monosultap using the nanosubstrate of β -CD/Trp/MgO/AgNPs which was MgO-protected silver nanoparticles (AgNPs) assembled tryptophan (Trp) and β -cyclodextrin ( β -CD). Six nanosubstrates including AgNPs, MgO/AgNPs, Trp/AgNPs, β -CD/MgO/AgNPs, Trp/MgO/AgNPs and β -CD/Trp/MgO/AgNPs were compared for their enhancement effect on monosultap. Among them, β -CD/Trp/MgO/AgNPs had the greatest enhancement effect, with an enhancement factor (EF) reaching up to 1.95 × 10 6 . The nanosubstrate of β -CD/Trp/MgO/AgNPs was characterized by various instrumental techniques, such as transmission electron microscopy (TEM), high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM), zeta potential, X-ray diffraction (XRD), thermogravimetric analysis (TGA), ultraviolet-visible (UV–vis) and Fourier transform infrared (FT-IR). The binding of β -CD/Trp/MgO/AgNPs with monosultap was studied in detail. The optimum experimental conditions were confirmed through single-factor experiments and response surface analysis. The interferences from the coexisting substances (common metal ions, glucose and congeneric drugs) were investigated to evaluate the method selectivity. Under the optimal conditions, the standard curve equation of monosultap was established within the concentration range of 2.00 × 10 −8 –1.00 × 10 −6 mol L −1 , with detection limit (LOD) of 0.970 nmol L −1 (3 σ / S ). The SERS approach was utilized for detecting monosultap in several vegetables and fruits with the recoveries from 96.3 % to 105 %.
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Shu Li,Tong Wang,Shu Ding,Xinyuan Guo,Yadi Liu,Shou-Qing Ni,Jing Chen,Jinhua Zhan
Surface-enhanced Raman spectroscopy (SERS) encounters difficulties in complex sample analysis due to the matrix interference caused by competitive adsorption on SERS substrates. To address this limitation, this work proposes an online-integrated paper centrifugation and SERS (PC-SERS) approach, using a circular paper disk coated with silver nanoparticles (P-AgNPs) as a separation medium and a SERS substrate. The device’s functionality is enabled by the differential migration behavior of substances on the P-AgNPs disk through rotation. Upon sample loading at the disk center, centrifugal force drives radial outward diffusion of analytes, solvent, and interferents. The enhanced diffusivity of solvent molecules relative to target analytes on P-AgNPs facilitates more expeditious adsorption of the analytes onto the substrate surface, resulting in higher SERS responses in a short time. Compared with conventional SERS analysis, the PC-SERS enhances signal intensity by 14-fold within a 20 min analysis. Mechanistic studies reveal that target analytes and interferents exhibit distinct interaction forces with the P-AgNPs, causing them to deposit at separated positions on the substrate during outward diffusion, thereby forming characteristic detection zones. PC-SERS effectively resists competitive adsorption, achieving a detection limit of 1 μg·L–1 for sulfonamide in the presence of strong interferents, which is 2 orders of magnitude lower than static SERS analysis. These enhanced capabilities enable reliable detection of trace sulfonamide antibiotics in complex matrices such as regenerated water (recovery of 84.12–105.27%) and vegetable extracts. Given the rapid analysis and portable operation requirements, PC-SERS is suggested to have strong potential for on-site environmental monitoring applications.
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高影响因子
PHYSICA SCRIPTA
·
2024
·Vol. 99
·期 10
·105604
·
IF 2.6
YangTao Yuan,ZhengXian Zhou,XuDong Hao,JiaJia Mi,Yu Cao,JianPing Shi
Surface-enhanced Raman scattering (SERS) is a spectroscopic technique that utilizes nanostructured substrates and is vital in food analysis. However, enhancing SERS substrates' sensitivity and applicability remains a challenge. The conductive metal organic framework Ni3(HHTP)2 has been widely used due to its excellent conductivity and porous structure. This study combines Ni3(HHTP)2's charge transfer properties with AgNPs's localized surface plasmon resonance to create a highly sensitive SERS substrate. Firstly, rod-shaped Ni3(HHTP)2 was prepared, and then a monolayer of AgNPs film was loaded on its surface. The substrate can detect crystal violet at a concentration of 10−14 M, and Ni3(HHTP)2 has a flat structure, coupled with AgNPs film, provided good detection uniformity (Relative Standard Deviation, RSD = 10.09%). Moreover, the substrate successfully detected food additives solutions, as well as the pesticide residues on fruit surfaces by bending. This substrate offers a rapid monitoring solution for food safety, facilitating real-time detection of contaminants in daily life.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Wen-Shu Zhang,Ya-Ning Wang,Zhang-Run Xu
Endogenous hydrogen sulfide (H 2 S) exists in multiple physiological processes . In order to further understand the action mechanism of H 2 S in cells and human body, we proposed a smart surface-enhanced Raman scattering (SERS) nanoprobe, Au core-4-mercaptobenzonitrile-Ag shell nanoparticle ( [email protected] @Ag), for the detection of endogenous H 2 S in living cells based on the reaction between Ag shell and sulfide species. 4-MBN was selected as the SERS reporter to avoid interference from cellular molecules. With the sulfide concentration increasing, the Ag 2 S constantly formed, and consequently the SERS signal intensity of [email protected] @Ag gradually decreased owing to the weaker SERS activity of Ag 2 S. With the nanoprobes, this method not only offers a high sensitivity for H 2 S detection at an nM level, but also achieves the goal of non-background analysis. It displays satisfactory anti-interference capability and a good linear relationship with sulfide concentration ranging from 50 nM to 500 μM, and an estimated detection limit is 0.14 nM. The [email protected] @Ag nanoprobes were successfully applied to detect endogenous H 2 S in living HepG2 cells stimulated by pyridoxal 5-phosphate monohydrate. This work offers a potential analytical method in the related research of H 2 S physiological function.
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PubMed
高影响因子
CLINICA CHIMICA ACTA
·
2025
·
IF 2.9
Biqing Chen,Zengkun Wang,Jiayin Gao,Haizhu Sun,Yinghan Zhao,Yan Liu,Jianyu Liu,Xiaohong Qiu
The occurrence and progression of endometrial cancer are closely associated with metabolic reprogramming, in which polyamine metabolites play a critical role in tumor cell proliferation and invasion. In this study, we developed a label free surface enhanced Raman scattering (SERS) detection platform based on gold nanostars (AuNS), integrated with machine learning algorithms, to achieve highly sensitive detection and precise identification of polyamine metabolites related to endometrial cancer. In complex biological matrices such as serum, the platform yielded stable and reproducible spectral fingerprints, with a detection limit at the nanogram level. Furthermore, by constructing a polyamine metabolite spectral database and introducing machine learning models, both the classification accuracy and AUC values exceeded 95 %, enabling effective discrimination of different metabolic states and mixed systems. Taken together, the AuNS SERS strategy combined with machine learning provides a rapid, non-invasive, and intelligent detection tool for the early diagnosis and metabolic subtyping of endometrial cancer, with significant clinical application potential.
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PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Xuanyu Sha,Siqingaowa Han,Guoqiang Fang,Nan Li,Dianyang Lin,Wuliji Hasi
In this work, a facile and effective method for assembly and transfer nanoparticles on thin layer chromatography (TLC) plate was proposed and used to combined with layer chromatography-surface-enhanced Raman spectroscopy (TLC-SERS) strategy for detection of Rhodamine B and Sudan I. First, Monodispersed Ag NCs with broadly tunable diameters (from 41 to 67 nm) and 7.25 × 10 5 maximum actual enhancement factor (AEF) were successfully prepared and systematically investigated for its optic performance use in TLC-SERS. Au@Ag NCs were monitored by TEM, SEM and UV–Vis spectrometer. The optical properties of Ag NCs were simulated and the experimental results showed good consistency with simulation results of absorption spectra. Through three phases interfacial technique and a simple transfer method, nanostructures were assembled on the TLC plate. Moreover, the semi-quantitative analysis of Rhodamine B and Sudan I in the range of 1–200 ppm was performed based on the assembled TLC plate with the limit of detection as low as 1 ppm. In addition, the practical feasibility of this strategy was verified by the detection of Sudan I and Rhodamine B in chili oil. In general, this assembled TLC-SERS strategy builds a connection for the application of nanomaterials with rich morphology in TLC-SERS, which has a great application prospect and practical application value.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 19
·4751-4761
·
IF 4.157
Cai Rongyuan,Lu Dechan,She Qiutian,You Ruiyun,Feng Shangyuan,Lin Xueliang,Lu Yudong
When nitrite is ingested and absorbed by the body, it can be converted into highly toxic nitrosamines (carcinogens, teratogens, and mutagens), posing health risks to the general population. Therefore, it calls for establishing a method for determination of nitrite. In this paper, the glass-SiO 2 -Ag surface-enhanced Raman scattering (SERS) substrate with a large number of “hot spots” were prepared by two kinds of silane coupling agents. The SERS substrate had high sensitivity and repeatability. Silicon dioxide supported the silver nanoparticles (Ag NPs), which increased surface roughness of the substrate, generated a great quantity of hot spots and enhanced the SERS signal. In the SERS spectrum, the intensity ratio of the two characteristic peaks (1287 cm −1 and 1076 cm −1 ) had a good linear correlation with the logarithm of the concentration of nitrite, R 2 = 0.9652. The recoveries of 50 μM and 100 μM nitrite in three kinds of foods, three kinds of cosmetics and tap water were 90.9–105.3%. Graphical abstract
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
·
IF 2.983
Yaohui Gao,Ziwei Hu,Jiamin Wu,Zan Ning,Jingyi Jian,Ting Zhao,Xiaochen Liang,Xingjie Yang,Zi Yang,Qinyu Zhao,Jianping Wang,Zhigang Wang,Nicoleta Elena Dina,Ana Maria Raluca Gherman,Zhengjin Jiang,Haibo Zhou
Palmatine is a protoberberine alkaloid separated from several plants and application as an anti-inflammatory and antibacterial agent in the therapy of gastrointestinal and genitourinary disorder. Thus, the fast quantification of palmatine is important in clinic medical assays. Herein, we report simple, fast and sensitive colorimetric visualization and surface-enhanced Raman spectroscopy (SERS) dual-mode detection of palmatine basing on bimetallic size tunable silver shell capped gold nanoparticles (Au@Ag NPs). Interesting, the best signals output for dual-mode sensing of palmatine were both 5 nm Ag shell thickness of Au@Ag NPs. Meanwhile, we found that the addition of NaHSO 4 significantly improves the aggregating sensitivity of Au@Ag NPs to trace palmatine. Upon exposure to 0.1 μM level palmatine, NaHSO 4 -optimized Au@Ag NPs solution exhibits a highly sensitive color change from orange to green and rapid aggregation kinetics within the initial 5 min, which can directly be seen with the naked eye and monitored by UV–vis absorbance spectra. In addition, we measured palmatine by SERS with the excellent enhancement effect of Au@Ag NPs for further increase the sensitivity and selectivity. More importantly, other protoberberine alkaloids do not interfere with this dual-mode sensor due to the different interaction force between Au@Ag NPs and these alkaloids, and the applicability of the sensor is well demonstrated in real samples with satisfactory results. This provide a fast and simple assay for the rapid detection of palmatine in traditional Chinese medicine, the limit of detection (LOD) is 0.13 μM by the naked eye and 0.10 μM by UV–vis spectroscopy. Therefore, the size-tunable of NaHSO 4 -optimized Au@Ag NPs can be used not only as a naked-eye sensor of palmatine, but also as a highly selective SERS probe.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Shuang Xiong,Tao He,Tao Zhou,Dalei Wang,Yueyue Wang,Chaoqing Dai,Wei Liu
Pesticides are often overused in production and life, which harms the environment and human health. However, the current tools for detecting pesticides are not effective enough due to lower flexibility and stability. With regards to this, we synthesized a flexible substrate of PDMS modified by MXene (Ti 3 C 2 Tx)/Ag. The MXene-noble metal composite substrate can detect 4-mercaptobenzoic acid (4MBA) at a low concentration of 1.0 × 10 −11 M. Furthermore, MXene can inhibit the oxidation of Ag nanoparticles (NPs) and efficiently localize the molecules to be tested within the hot spots. In particular, the flexibility of the substrate enables the detection of trace amounts of thiabendazole (TBZ) by collecting samples directly from uneven surfaces such as fruits and vegetables. The substrate is also capable of detecting a mixture of TBZ and 4MBA simultaneously. Using intrinsic Raman peak of PDMS as the internal standard can eliminate the influence of environmental factors on the detection results, which makes the detection results more quantitative. The resultant noble metal-semiconductor substrates show great potential applications in food safety and environmental monitoring fields.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Abid Hussain,Hongbin Pu,Da-Wen Sun
A cysteamine functionalized core shelled nanoparticles (Au@Ag-CysNPs) was presented for simultaneous and rapid detection of sodium thiocyanate (STC) and benzoic acid (BA) preservatives in liquid milk using surface-enhanced Raman spectroscopy (SERS) technique. A spectrum covering 350–2350 cm −1 region was selected to detect STC with concentrations ranging from 0.5 to 10 mg/L and BA with concentrations ranging from 15 to 240 mg/L in milk samples. Characterization of nanoparticles using high-resolution TEM confirmed that the successful synthesis of Au@AgNPs with core (gold) size of 28 nm and shell (silver) thickness of about 5 nm was grafted with 120 μL of 0.1 nM cysteamine hydrochloride. Results showed that Au@Ag-CysNPs could be used to detect STC up to 0.03 mg/L with a limit of quantification (LOQ) of 0.039 mg/L and a coefficient of determination (R 2 ) of 0.9833 in the milk sample. For detecting BA, it could be screened up to 9.8 mg/L with LOQ of 10.2 mg/L and R 2 of 0.9903. The proposed substrate was also highly sensitive and the employed method involved only minor sample pretreatment steps. It is thus hoped that the new substrate could be used in the screening of prohibited chemicals in complex food matrices in future studies.
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高影响因子
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
·
2018
·
IF 2.701
Xue-Qin Zhang,Sheng-Xian Li,Zeng-Ping Chen,Yao Chen,Ru-Qin Yu
Due to the practical impossibility in controlling the number and distribution of "hot spots" on or close to the surfaces of enhancing substrates, internal standards are generally used to improve the accuracy and precision of quantitative surface-enhanced Raman spectroscopy (SERS). Traditional internal standard methods based on either univariate ratiometric models or conventional multivariate calibration models can use the information of only one characteristic SERS peak of only one internal standard, which weakens the efficiency of traditional internal standard methods in improving the quantitative results of SERS assays. In this contribution, the concept of using multiple internal standards was introduced for the first time to quantitative SERS assays by incorporating a unique spectral shape deformation (SSD) quantitative theory with multiple-internal-standard embedded core-shell nanoparticles . The quantification of phosmet residues on apple skins by SERS technique with Au-core/Ag-shell nanoparticles embedded with both 2-MB and PATP as enhancing substrate was used to illustrate the applicability of the proposed strategy. Experimental results demonstrated that the proposed multiple-internal-standard strategy based on SSD achieved much more accurate and precise concentration predictions for phosmet residues on apple skin than traditional single-internal-standard methods based on either univariate ratiometric calibration models or conventional multivariate calibration models. The SERS quantitative results of the multiple-internal-standard strategy were comparable to those obtained by LC-MS/MS. The average relative deviation between the quantitative results of the proposed method and those of ICP-MS was about 8%. The good accuracy of the proposed method makes it a promising alternative for quantitative SERS assays.
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高影响因子
NANOTECHNOLOGY
·
2016
·Vol. 27
·期 39
·395304
·
IF 3.573
Honghua Zhang,Dilong Liu,Lifeng Hang,Xinyang Li,Guangqiang Liu,Weiping Cai,Yue Li
A facile route has been proposed for the fabrication of morphology-controlled periodic SiO2 hierarchical micro/nanostructured arrays by reactive ion etching (RIE) using monolayer colloidal crystals as masks. By effectively controlling the experimental conditions of RIE, the morphology of a periodic SiO2 hierarchical micro/nanostructured array could be tuned from a dome-shaped one to a circular truncated cone, and finally to a circular cone. After coating a silver thin layer, these periodic micro/nanostructured arrays were used as surface-enhanced Raman scattering (SERS)-active substrates and demonstrated obvious SERS signals of 4-Aminothiophenol (4-ATP). In addition, the circular cone arrays displayed better SERS enhancement than those of the dome-shaped and circular truncated cone arrays due to the rougher surface caused by physical bombardment. After optimization of the circular cone arrays with different periodicities, an array with the periodicity of 350 nm exhibits much stronger SERS enhancement and possesses a low detection limit of 10−10 M 4-ATP. This offers a practical platform to conveniently prepare SERS-active substrates.
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高影响因子
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