APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 102
·期 13
·5695-5705
·
IF 3.34
Qiao Ying,Cai Hao-Liang,Yang Xi,Zang Yuan-Yuan,Chen Zhi-Gang
In this study, a new method of cryopreservation of lactic acid bacteria (LAB) using natural deep eutectic solvents (NADESs) was developed. Survival rates of LAB during 24-h short-term and 180-day long-term cryostorage at − 20 °C were investigated. The results revealed that survival of Streptococcus thermophilus ( S. thermophilus ) in NADESs after 24 h of cryostorage was superior to survival of two other tested LAB. Moreover, survival was higher at a ratio of NADESs to S. thermophilus of 1:1 ( v / v ) than observed using a 4:1 ( v / v ) ratio. Representative freezing characteristics of five NADESs were elucidated, including thermodynamic properties and hydrogen bonding interactions after addition of water. In order to identify the protective mechanism of NADESs on cell structure and vital metabolic enzymes of S. thermophilus during cryostorage, transmission electron microscopy (TEM) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were employed and enzyme activities of both lactate dehydrogenase (LDH) and β -galactosidase were determined. Subsequently, NADES GlyP prepared from glycerol and L-proline was demonstrated to maintain cell membrane structural integrity and significantly ( p < 0.05) maintain activities of both intracellular enzymes of S. thermophilus . Moreover, NADESs could efficiently penetrate S. thermophilus cells and intracellular β -galactosidase activity could be used to demonstrate NADESs effectiveness in maintaining S. thermophilus survival after long-term cryostorage. These results demonstrate that NADESs can be used as green cryoprotective chemical agents (CPAs) that can efficiently increase LAB viability during cryostorage. These results should have great value in the food production and probiotics industries.
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高影响因子
Nanoscale Research Letters
·
2012
·Vol. 7
·期 1
·1-7
·
IF 2.726
Zhang Jun,Zhao Su-Qing,Zhang Kun,Zhou Jian-Qing,Cai Yan-Fei
In the present work, ZnO quantum dots (QDs) have been prepared by the sol–gel method, and the performance of the QDs has been improved. The effect of Cd concentration on the structural and luminescent properties of the QDs, as well as the effect of the mass ratio of trioctylphosphine oxide (TOPO)/octadecylamine (ODA), has been investigated. The ZnO and Cd-doped ZnO QDs have hexagonal wurtzite structures and are 3 to 6 nm in diameter. When the Cd content was increased, the QD particle size was reduced; this effect was confirmed in the corresponding ultraviolet–visible spectra. The fluorescence intensity was simultaneously enhanced significantly. Both the UV and fluorescence spectra were blue-shifted. The luminous intensity was further enhanced when the QDs were modified with TOPO/ODA. Fourier transform infrared and X-ray diffraction techniques proved that the polymer successfully coated the surfaces of the QDs. A TOPO/ODA mass ratio of 1:2 was determined to result in the best optical performance among the different ratios examined. The results showed that the described synthetic method is appropriate for the preparation of doped QDs with high-fluorescence quantum efficiency.
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高影响因子
Journal of Innovative Optical Health Sciences
·
2022
·
IF 2.396
Yuting Zhang,Guojing Li,Jiong Li,Ming Wu,Xiaolong Liu,Jingfeng Liu
The discovery of aggregation-induced emission (AIE) effect provides opportunities for the rapid development of fluorescence imaging-guided photodynamic therapy (PDT). In this work, a boron dipyrromethene (BODIPY)-based photosensitizer (ET-BDP-O) with AIE characteristics was developed, in which the two linear arms of BODIPY group were linked with triphenylamine to form an electron Donor–Acceptor–Donor (D–A–D) architecture while side chain was equipped with triethylene glycol group. ET-BDP-O was able to directly self-assemble into nanoparticles (NPs) without supplement of any other matrices or stabilizers due to its amphiphilic property. The as-prepared ET-BDP-O NPs had an excellent colloid stability with the size of 125 nm. Benefiting from the AIE property, ET-BDP-O NPs could generate strong fluorescence and reactive oxygen species under light-emitting diode light irradiation (60 mW/cm 2 ) . After internalized in cancer cells, ET-BDP-O NPs were able to emit bright red fluorescence signal for bioimaging. In addition, the cell viability assay demonstrated that the ET-BDP-O NPs exhibited excellent photo-cytotoxicity against cancer cells, while negligible cytotoxicity under dark environment. Thus, ET-BDP-O NPs might be regarded as a promising photosensitizer for fluorescence imaging-guided PDT in future.
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高影响因子
TALANTA
·
2012
·
IF 3.794
Yafei Yang,Jiarun Tu,Wensheng Cai,Xueguang Shao
A method for quantitative determination of fish sperm deoxyribonucleic acid (fsDNA) in solutions was developed by using adsorption preconcentration and near-infrared diffuse reflectance spectroscopy (NIRDRS). A high capacity adsorbent of amino-modified silica particle (AMSP) was prepared for preconcentration of fsDNA in solutions. Under the optimized conditions, the adsorption rate can be above 90% within 3 min. After adsorbing the DNA onto the adsorbent, near-infrared (NIR) spectra in diffuse reflectance mode were measured and partial least squares (PLS) model was established for fast quantitative prediction. The results show that the correlation coefficient ( R ) between the predicted and the reference concentration is 0.9894 and the recoveries are in the range of 92.9–123.4% for the validation samples in the concentration range of 3.00–29.38 mg L −1 . Therefore, the feasibility for quantitative analysis of DNA in solutions by NIRDRS is proved. This may provide an alternative way for fast determination of DNA in solutions.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Tong Wang,Yongyi Zhang,Zhizhou Ling,Ying He,Jinyi Yang,Yuanxin Tian,Yu Wang,Hong Wang,Zhenlin Xu,Yuanming Sun,Yudong Shen
Imazalil is an imidazole fungicide that controls fruit diseases by inhibiting ergosterol synthesis but has resulted in residues in food. There is a need to develop rapid immunoassays suitable for on-site food safety monitoring and primary screening of large samples. The development of high-performance antibodies represents a pivotal front-end process in the establishment of immuno-rapid assays for harmful small molecules. This study innovatively adopted a hapten preparation strategy by introducing a quaternary ammonium cationic arm to enhance specific immune responses without exposing the common fragment imidazole ring. Theoretical chemistry techniques, including electrostatic potential, lipophilic potential, molecular structure superposition, and frontier molecular orbital energy gap-based molecular softness, were used to analyze the structural features of the quaternary ammonium cationic hapten IMZ-A. The results showed that IMZ-A retained imazalil’s microelectronic and macroscopic three-dimensional spatial superposition structures and showed increased softness for higher bioreactivity. The obtained imazalil monoclonal antibody demonstrates high specificity and sensitivity, with an IC 50 of 1.96 ng mL −1 . A colloidal gold immunochromatographic assay was developed, detecting imazalil residues in fruit samples within 7 min, with a qLOD of 0.57–2.05 ng mL −1 and an IC 50 of 3.67–12.41 ng mL −1 in citrus, apple, and banana samples. This work offers a convenient and highly sensitive immunoassay for large-scale on-site detection of trace imazalil residues. More significantly, it provides a novel and general design idea for hapten, namely, introducing cations into hapten molecules to improve their specific immune potential.
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高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2024
·
IF 2.3
Yiwan Shi,Zhaozhen Wang,Weikang Xu,Xiaolu Yu,Botao Gao,Xinting Zhou,Jiwen Chen,Kunfeng Jia,Lek Hang Cheang,Man seng Tam,Huajun Wang,Xiaofei Zheng,Tingting Wu
Large bone defect repair is a striking challenge in orthopedics. Currently, inorganic-organic composite scaffolds are considered as a promising approach to these bone regeneration. Silicon ions (Si4+) are bioactive and beneficial to bone regeneration and Si4+-containing inorganic mesoporous silica (MS) can effectively load drugs for bone repair. To better control the release of drug, we prepared biodegradable MS/PLGA (MP) microspheres. MP loaded organic silk fibroin/carboxymethyl chitosan/sodium alginate (MP/SF/CMCS/SA) composite scaffolds were further constructed by genipin and Ca2+ crosslinking. All MP/SF/CMCS/SA scaffolds had good swelling ability, degradation rate and high porosity. The incorporation of 1% MP significantly enhanced the compressive strength of composite scaffolds. Besides, MP loaded scaffold showed a sustained release of Si4+ and Ca2+. Moreover, the release rate of rhodamine (a model drug) of MP/SF/CMCS/SA scaffolds was obviously lower than that of MP. When culturing with rat bone marrow mesenchymal stem cells, scaffolds with 1% MP displayed good proliferation, adhesion and enhanced osteogenic differentiation ability. Based on the results above, the addition of 1% MP in SF/CMCS/SA scaffolds is a prospective way for drug release in bone regeneration and is promising for further in vivo bone repair applications.
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Juan Zhu,Jian Liu,Yao Fan,Meixia Wu,Chunsong Zhou,Haiyan Fu,Yuanbin She
Anthraquinone dyes have been widely used to color textile fibers since antiquity. Identification of the dyes can help us understand the dyeing processes and when and where the textiles were produced. Herein, we present a strategy based on surface-enhanced Raman scattering (SERS) with a novel silver colloid substrate for the detection of anthraquinone dyes. Quasi-spherical silver particles with different sizes were prepared by the solvothermal method and then characterized by transmission electron microscopy (TEM). The silver colloid substrates exhibited high-density hot spots with good reproducibility (RSDs of 3 ∼ 16 %) and high sensitivity. Among them, Ag- C 2 (the molar ratio of AgNO 3 to PVP is 0.367, reacted for 2 h) was used to detect anthraquinone dyes in reference silk fibers as well as ancient textile samples due to the highest sensitivity and the low RSD (5.37 %) in this study. More importantly, Ag- C 2 can be utilized to distinguish three madder species ( Rubia tinctorum , Rubia cordifolia, and Rubia argyi ) depending on the SERS intensity of alizarin and purpurin.
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高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Ziwei Lin,Sheng Wang,Qing Yan,Qinghua Yan,Derong Cao
Light is not the only stimuli which can coverts donor-acceptor Stenhouse adducts (DASAs) from the triene to the cyclopentenone form. Here, we firstly demonstrate that Cu 2+ can drive linear-to-cyclic isomerization of DASAs in acetonitrile , which increase the rate of color fading. Three different generations of DASAs ( DASA-1 , DASA-2 , and DASA-3 ) have been synthesized by Knoevenagel condensation to verify the universal applicability of the mechanism by which copper ions discolor DASAs. The Job's plot of DASA-1 to Cu 2+ indicated DASA-1 binds to Cu 2+ with a ratio of 1:1, and the 1 H NMR titration spectrum of Cu 2+ on DASAs as well as the UV–Vis absorption spectra with adding Cu 2+ showed that copper ions could induce the process of linear-to-cyclic isomerization of DASAs in acetonitrile. Based on this, we propose copper ions as the eraser of a DASA derived ink to achieve repeatable erasing. Furthermore, DASAs have been utilized as a Cu 2+ sensor with high selectivity and sensitivity.
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高影响因子
TALANTA
·
2012
·
IF 3.794
Wen Lei,Zihui Meng,Weibing Zhang,Lingyi Zhang,Min Xue,Wei Wang
Molecular imprinting is a useful method to make enzyme mimics for protein recognition. Classic protein imprinting involves entrapping proteins within polysiloxane or polyacrylamide , but due to the rigidity of the recognition sites and the limited interaction between proteins and small molecule monomers , they often have unsatisfactory capacity, poor reproducibility and low specificity. In this report, “soft” and flexible recognition sites that can allow “induced fit” of the target proteins were created by a novel surface imprinting technique. When about 25% of the template proteins were removed, the unremoved proteins created “soft” and flexible loops that can lock into place upon protein rebinding, which provides additional favorable interactions between the rebind proteins and the imprinted sites. The adsorption capacity of the surface imprinted silica is 24.8×10 −7 mol/g. The “soft” recognition sites can distinguish target hemoglobin from other proteins such as bovine serum albumin, Cytochrome C and RNase A.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Dong-Mei Liu,Juan Chen,Yan-Ping Shi
In the present study, the immobilization method of physical adsorption combined with covalent crosslinking was developed to avoid the shortcomings of both the noncovalent and covalent coupling methods. For the first time, tyrosinase (TYR) was immobilized on the surface of aminated magnetic nanoparticles (Fe 3 O 4 -NH 2 ) by the developed method. TYR was firstly adsorbed on the surface of Fe 3 O 4 -NH 2 by electrostatic interaction , and then by covalent crosslinking with glutaraldehyde (GA), TYR was firmly immobilized on the supports. The immobilized TYR showed enhanced pH and temperature endurances at the optimum pH of 7.0 and temperature of 35 °C. TYR reusability was significantly improved after immobilization and it retained 61.4 ± 2.3% of its initial activity after 5 repeated cycles. Immobilized TYR also showed improved storage stability (73.2 ± 1.1% after 30 days of storage at 4 °C). In addition, the immobilized TYR showed a higher biological affinity to substrate owing to the stabilization of TYR in its active conformation by electrostatic interaction prior to covalent crosslinking. Finally, the immobilized TYR was used to screen of enzyme inhibitors from 11 traditional Chinese medicines (TCMs) to validate whether this method can be used for enzyme inhibitor screening or not.
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高影响因子
APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Ting Gong,Yu Huang,Lijun Qin,Wangle Zhang,Jianguo Li,Longfei Hui,Hao Feng
In the chemical industry palladium-based catalysts are commonly used to remove trace amounts of acetylene from ethylene feed. In this work atomic layer deposition (ALD) is utilized to synthesize uniformly dispersed Pd nanoparticles inside the meso-pores of a silica molecular sieve MCM-41. The space confinement effect from the internal channels of the molecular sieve leads to a very narrow size distribution (2–3 nm) of Pd nanoparticles and effectively prevents the particles from sintering during high temperature treatments. In selective hydrogenation of acetylene the ALD Pd nanoparticle catalysts exhibit excellent catalytic performances under ambient temperature and pressure. The excellent performance of the ALD catalyst is attributed to small Pd nanoparticles uniformly dispersed in the meso-pores of MCM-41 so that more Pd surface sites are available for the catalytic reaction. The strong metal support interaction between Pd and TiO 2 can be induced by high temperature treatments, which suppresses the surface adsorption of both acetylene and ethylene, leading to decreased acetylene conversion and improved ethylene selectivity . The high temperature treatment also helps improve the stability of the catalysts.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2012
·
IF 4.531
Jiaheng Zhang,Min Li,Miyi Yang,Bing Peng,Yubo Li,Wenfeng Zhou,Haixiang Gao,Runhua Lu
A novel, rapid ionic liquid dispersive liquid–liquid microextraction (IL-DLLME) technique combined with magnetic retrieval (MR-IL-DLLME) has been developed and used to analyze five benzoylurea insecticides (BUs) in environmental water samples. This procedure was based on the magnetic retrieval of the ionic liquid using unmodified magnetic nanoparticles (MNPs). In this experiment, the fine ionic liquid droplets formed in aqueous samples functioned as an extractant for the extraction of BUs; after the extraction process was completed, Fe 3 O 4 MNPs were added as a carrier to retrieve and separate the ionic liquid from the sample solution. After the supernatant was removed, the ionic liquid was desorbed using acetonitrile and subsequently injected directly into an HPLC system for analysis. The optimum experimental parameters are as follows: 20 mg of Fe 3 O 4 (20 nm) as magnetic sorbents; 70 μL of [C 6 MIM][PF 6 ] as the extraction solvent; 300 μL of acetonitrile as the disperser solvent; a vortex extraction time of 90 s with the vortex agitator set at 2800 rpm and no ionic strength. Under the optimized conditions, good linearity was obtained with correlation coefficients ( r ) greater than 0.9981. The repeatability and reproducibility of the proposed method were found to be good, and the limits of detection ranged from 0.05 μg L −1 to 0.15 μg L −1 . The proposed method was then successfully used for the rapid determination of BUs in real water samples. The recoveries of five BUs at two spiked levels ranged from 79.8 to 91.7% with RSDs less than 6.0%.
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高影响因子
危险品化学品经营许可证（带存储）