Frontiers in Microbiology
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2025
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IF 4.5
Yue Gao,Binbin Huang,Yunyi Qin,Bing Qiao,Mengfei Ren,Liqing Cao,Yan Zhang,Maozhen Han
Background: Obesity and high fasting blood glucose (FBG) resulting from high-fat diets (HFDs) have emerged as significant public health concerns, garnering increasing attention. Recently, gut microbiota has been linked with metabolic diseases such as type 2 diabetes (T2DM), and its mediating role in dietary supplements has been confirmed. Seeking various dietary supplements to lose body weight (BW) and decrease FBG and explaining the underlying mechanism have become the research hotspots in T2DM studies.Methods: In this study, rutin and proanthocyanidins (PA) were selected as dietary supplements (200 mg/kg × day, oral gavage, 6 weeks) in T2DM mice induced with HFD to assess their efficacy in weight loss, FBG reduction, gut microbiota alterations, and the associated underlying mechanisms.Results: Our findings indicate that rutin was more effective than PA in relieving inflammation and fat hypertrophy, although both significantly reduced BW and FBG within 2 weeks after the intervention. Analysis of 16S rRNA amplicons revealed substantial alterations in the gut microbial community composition of mice administered with PA and rutin compared to HFD-fed mice. Importantly, several core microbes, particularly a series of probiotics, such as Akkermansia, Lactococcus, Odoribacter, Faecalibaculum, and Roseburia were identified, which were significantly correlated with the changes in BW and FBG.Conclusion: Overall, our study highlights that rutin and PA can reduce BW, FBG, and inflammation by modulating the gut microbiota composition, providing novel perspectives for managing and treating weight and FBG concerns in obesity and T2DM patients through dietary supplements in clinical treatment.
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ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Abdelhadi El Jaouhari,Luyun Yan,Jinhua Zhu,Dongbao Zhao,Md. Zaved Hossain Khan,Xiuhua Liu
Exceptional sensitivity behavior of molecularly imprinted polymer (MIP) approach towards an electrochemical sensor application encourages combining this approach with other materials to tune their properties such as large surface area, high catalytic effect, electrical, thermal and mechanical stability for the fabrication of sensors with high sensitive performance. In the present work, highly sensitive MIP sensor was developed and successfully applied for rutin recognition. The MIP was decorated on the surface of zeolitic imidazolate framework (ZIF-8) and reduced graphene oxide (rGO) composite modified with glassy carbon electrode (GCE) to fabricate GCE/rGO/ZIF-8/MIP electrode. Spectroscopic and microscopic analyses such as XRD , FT-IR, BET and SEM were used to evaluate the composition and the morphology of the surface of GCE/rGO/ZIF-8/MIP electrode. The electrochemical characterization of electrodes was performed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Also different parameters influencing the sensitivity of GCE/rGO/ZIF-8/MIP, such as the percentage of rGO, template:monomer ratio, number of electropolymerization cycles, accumulation time and pH were optimized. Under optimal conditions, the MIP sensor shows a wide linear range and low limit of detection as well as good reproducibility, stability and selectivity , and used successfully for the determination of rutin in real solutions.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 6
·1-10
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IF 5.3
Wu Jun,Du Hao,Zhu Xiashi
A sensitive and selective fluorescence sensor for rutin was successfully developed. The sensor was fabricated from highly fluorescent nitrogen-boron co-doped carbon quantum dots (N,B-CDs) coated with mesoporous silica molecularly imprinted polymer (MIP). Rutin was detected by this sensor (N,B-CDs @ MIP) with a linear range of 0.08–30 μM and a detection limit as low as 0.05 μM, under optimal conditions. Meanwhile, the usefulness of the developed sensor was evaluated in samples of lake water, orange, and honey with satisfactory recoveries. Graphical
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TALANTA
·
2012
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IF 3.794
Huan Zeng,Yuzhi Wang,Xiaojie Liu,Jinhuan Kong,Chan Nie
Molecular imprinted polymers (MIPs) were prepared using rutin as the template, different reagents as the functional monomer and different reagents as the cross-linker by solution polymerization. Several parameters that would influence the performance of MIPs were investigated including the type of functional monomer (single or double) and cross-linker (single or double), and the molar ratio of the template, the functional monomer and the cross-linker. The optimum synthesis conditions of MIPs were found to be bi-monomers (acrylamide-co-2-vinyl pyridine, 3:1) and bi-crosslinker (ethylene glycol dimethacrylate-co-divinylbenzene, 3:1). The ratio of the template, the functional monomer and the cross-linker was found to be 1:6:20. MIPs synthesized under these conditions were filled into the cartridges as the adsorbents of solid-phase extraction (SPE). A competition test was conducted to authenticate the selectivity and the specificity of molecularly imprinted solid-phase extraction (MISPE) for rutin using the mixture solution of standard rutin and its structural analogs including quercetin, naringenin and kaempferol. Compared with purchased SPE including C 18 , silica and PCX, MISPE showed better selectivity and enrichment property for rutin in the extracted solutions of Chinese medicinal plants than any others. The mean recoveries were 85.93% (RSD: 3.04%, n = 3) for Saururus chinensis (Lour.) Bail and 88.61% (RSD: 3.36%, n = 3) for Flos Sophorae, respectively, which indicated that the optimized rutin -MIPs possess the value of practical application.
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AQUACULTURE NUTRITION
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2026
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IF 3.9
Ke Cheng,Xinyao Zhang,Lixue Dong,Di Peng,Yangyang Liu,Dexing Zhu,Zhongbao Guo,Yongju Luo,Apeng Liu,Juan Tian,Hua Wen,Mingdian Liu,Ming Jiang
A high‐level fava beans diet has been proven to enhance the texture characteristics of tilapia muscle, yet it can also induce hepatointestinal injury. Rutin, as a nutritional additive, has antioxidant and immune‐boosting effects. This study explored the regulatory effect of 150 and 300 mg/kg rutin on the muscle characteristics and hepatointestinal health of tilapia after being fed with a 60% fava beans diet. Two hundred and forty tilapias (average weight: 371.50 ± 9.29 g) were evenly divided into four groups and fed with the diet of groups C0 (0% fava bean), R0 (60% fava bean), R1 (60% fava bean + 150 mg/kg rutin), and R2 (60% fava bean + 300 mg/kg rutin) for 10 weeks, respectively. The results indicated that the growth performance in R1 and R2 showed no significant changes compared to R0, while muscle hardness, gumminess, and resilience increased, along with an elevation in muscle crude protein deposition. The addition of 150 and 300 mg/kg rutin enhanced the antioxidant capacity (AOC) of the muscles, liver, and intestines, alleviated liver damage by regulating liver lipid metabolism compared with R0. Dietary supplementation with 150 mg/kg rutin improved the composition of intestinal microbiota, significantly upregulating the abundance of beneficial bacteria Muribaculaceae unclassified and reduced the abundance of harmful bacteria Rhodobacteraceae , Chloroplast_unclassified , and Rothia . In summary, rutin can effectively function as a nutritional supplement to alleviate liver and intestinal damage caused by 60% fava bean feed, while also improving muscle texture simultaneously. This study provides a crucial theoretical basis for optimizing crispy tilapia aquaculture through rutin supplementation, promoting innovation in aquatic feed formulations.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2014
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IF 3.84
Suling Yang,Gang Li,Guifang Wang,Junhong Zhao,Zhihui Qiao,Lingbo Qu
Through a simple, rapid and green method, a small size of yttrium hexacyanoferrate nanoparticles (YHCFNPs) was synthesized firstly. As the novel sensing materials, a sensor of YHCFNPs/chemically reduced graphene oxide (CRGO) nanocomposite modified carbon paste electrode (CPE) was fabricated. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), inductive coupled plasma emission spectrometer (ICPES) and electrochemistry were performed to characterize the modified electrode. SEM images showed that the average diameter of the prepared YHCFNPs was about 40 nm. The electrochemical behavior of the YHCFNPs/CRGO/CPE in response to rutin was studied. The response current of rutin at the YHCFNPs/CRGO/CPE was obviously higher than that at the other modified CPE. Under the optimal conditions, YHCFNPs/CRGO/CPE exhibited a broader detection range from 2.0 to 4000 nmol/L with a nanomolar detection limit of 0.82 nmol/L ( S / N = 3). This novel method was successfully used to determine rutin in pharmaceutical tablets.
研究领域:
分析化学
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电化学传感
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纳米材料
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药物分析
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生物医学工程
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ACS Nano
·
2023
·
IF 17.1
Hao Wang,Feng Lin,Yi Wu,Wei Guo,Xuesi Chen,Chunsheng Xiao,Meiwan Chen
Spinal cord injury (SCI), which is characterized by excessive inflammatory cell infiltration and accumulation of oxidative substance, would severely impede neurological functional recovery and lead to permanent and profound neurologic deficits and even disability. Methylprednisolone (MP) is the most commonly used clinical anti-inflammatory drug for SCI treatment, but high doses are typically required that can cause severe side effects. Here, we developed a carrier-free thioketal linked MP dimer@rutin nanoparticles (MP2-TK@RU NPs) which can achieve combined SCI treatment by coassembling reactive oxygen species (ROS) cleavable MP dimers and rutin. This proposed nanodrug possesses the following favorable advantages: (1) the carrier-free system is easily accessible and has a high drug-loading capacity, which is preferred by the pharmaceutical industry; (2) The ROS-cleavable linker increases the efficiency of targeted drug delivery to the injury site; (3) Rutin, a type of plant-derived natural flavonoid with good biocompatibility, anti-inflammatory, and antioxidant properties, is codelivered to enhance the therapy outcomes. The obtained MP2-TK@RU NPs exhibited potent anti-inflammatory and antioxidative properties both in vitro and in vivo, demonstrating superior locomotor function recovery and neuroprotective efficacy in rats with SCI. This carrier-free nanodrug is anticipated to provide a promising therapeutic strategy for clinical SCI treatment.
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Xuqian Ma,Fangqi Wang,Taijun Hang,Pierre Dramou
Compound structure change of the template molecule from rutin samples might affect the selectivity and adsorption of molecularly imprinted polymers based on magnetic halloysite nanotubes (MHNTs@MIPs). In the present study, not only MHNTs@MIPs were successfully characterized by TEM, SEM, EDS, FT-IR, TGA and VSM, but related compounds and potential degradation factors of template molecule rutin were also investigated in the polymerization and elution process of MHNTs@MIPs by high-performance liquid chromatography together with photodiode array and tandem mass spectrometry (HPLC-PDA-MS/MS) in negative ion mode. Nine flavonol components were detected in rutin active pharmaceutical ingredient samples, such as rutin, isoquercetrin, kaempferol-3- O -rutoside, isorhamnetin-3- O -rutoside, quercetin, kaempferol, isorhamnetin and two unknown triglycosides. Under acidic, basic, oxidative and solvent conditions, two common degradants with negative ESI-MS ions at m/z 317 and 349 were found. Meanwhile, protocatechuic acid and methyl ester were also observed as two characteristic oxidative degradants. Compared to above degradation results, rutin-dimethylsulfoxide adduct and oxidative degradant were observed in the synthesis supernatant, which demonstrated that appropriate solvents and strict control of the oxygen level were critical in the synthesis process. Therefore, degradation studies provide a solid foundation for the optimization of MHNT@MIP synthesis with rutin as a template, which can be potentially applied to other templates for further material performance investigation.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Fangqi Wang,Xu Ni,Jingjing Zhang,Qikun Zhang,Huning Jia,Hua He,Pierre Dramou
In this work, with the attempt to further improve the selectivity, magnetism and loading proportion of existing adsorbents, a novel composite (MGO/MHNTs@MIPs) was synthesized by electrostatically combining molecularly imprinted polymer based on the surface of magnetic halloysite nanotubes (MHNTs@MIPs) with magnetic graphene oxide (MGO). Then some characterizations were done to prove its successful synthesis. Besides, the bonding experiment showed that it possessed a loading capacity of up to 132 mg·g −1 , and the adsorption behavior of MGO/MHNTs@MIPs was elucidated by Langmuir isotherm model and Pseudo-second order model. By comparing its adsorption capacity to analogues, we concluded that the MGO/MHNTs@MIPs with the MHNTs@MIPs as basic elements exhibited higher selectivity (imprinting factor = 2.25) than that of MGO/MHNTs@NIPs based on MHNTs@NIPs for template rutin. Furthermore, a series of solid phase extraction conditions were optimized, and then the materials were used for the extraction and detection of rutin in fruit juice under the optimal conditions.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Huijia Song,Haipin Zhang,Yulian He,Ruixia Gao,Yue Wang,Wenting Wang,Lisa D. Pfefferle,Xiaoshuang Tang,Yuhai Tang
Rutin (RT), a widely distributed natural flavonoid compound, has been generally utilized as an important active ingredient owing to its considerable biomedical and economic value. Inspired by the structure features of densely-packed bayberry and well-orientated honeycomb, a novel type of magnetic molecularly imprinted polymers (HB-TI-MMIPs) with abundant high-affinity and uniformly-distributed binding sites was rationally constructed for the selective enrichment of RT from Sophora japonica. The polymerization conditions, physicochemical properties, and adsorption performance of the imprinted nanomaterials were systematically investigated. The optimized HB-TI-MMIPs display a high adsorption capacity, fast adsorption rate, and satisfactory selectivity towards RT. Meanwhile, the proposed analytical methodology using HPLC, with HB-TI-MMIPs as adsorbents, successfully applied to enrich and detect RT from Sophora japonica with high recoveries (87.2–94.6%) and good RSDs (lower than 4.3%). Therefore, the fabricated HB-TI-MMIPs with a fast magnetic responsivity and desirable adsorption performance would be attractive in plant active ingredients extraction fields.
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FOOD AND CHEMICAL TOXICOLOGY
·
2020
·
IF 4.679
Chunlei Zhu,Fengru Liu,Yunlong Wei,Fan Zhang,Ting Pan,Yingwang Ye,Yizhong Shen
Having reported that rare earth elements displayed potential toxicity i n vivo , often be found in soil, plants and etc. , which might be easily chelated with the natural functional molecule rutin to form rutin metal complexes, ultimately entering the human body by means of food chain . However, few reports paid the attention on the toxicology of the complexes consisting of rutin with rare earth ions. Here, we focused on the potential toxicity by probing the site-selective binding of the rutin-rare earth ions complexes to human serum albumin (HSA). As a proof-of-concept, we selected Pr 3+ as the representative to conjugate with rutin to form rutin-Pr(III) complex, which was further applied to interact with HSA in aqueous solution. The results exhibited that the rutin-Pr(III) complex primary bound to the hydrophobic cavity at site II (subdomain IIIA) of HSA through hydrogen bonding and van der Waals force . Through the thermomechanical analysis, we found this binding process was spontaneous because of the negative Δ G. We believe that this work may offer a new insight into understanding the physiological effects ( e.g. toxicology) of rutin and rare earth ions, which could be helpful to guide their rational use in the agriculture and environment-related industries.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yixia Lu,Jian Hu,Yanbo Zeng,Ying Zhu,Hailong Wang,Xiaoling Lei,Shisi Huang,Longhua Guo,Lei Li
A novel electrochemical sensor for rutin determination based on molecularly imprinted poly (ionic liquid) (MIPIL)/ionic liquid-graphene (IL-GR) modified electrode was developed. MIPIL was synthesized via free radical polymerization using rutin as the template, 1-allyl-3-ethyl imidazolium bromide ([AEIm]Br) as the functional monomer and 1,4-butanediyl-3,3′-bis- l -vinylimidazolium dibromide ([V 2 C 4 (mim) 2 ]Br 2 ) as the cross-linker. The composite of IL-GR as an electrode sensitive element was prepared using carboxymethyl-3-methylimidazolium chloride ([HO 2 CMMIm]Cl) by one-step ultrasound method. Fourier transform infrared spectroscopy and scanning electron microscope were used to characterize IL-GR and MIPIL. The increased surface area and conductivity of IL-GR/GCE improved the sensitivity of rutin sensor. Under the optimum condition, good linearity for rutin analysis by the MIPIL-based sensor was obtained from 0.03 to 1 μM. A low limit of detection for rutin was 0.01 μM (S/N = 3). The MIPIL-based sensor demonstrated superiority on DPV response compared to the imprinted sensors based on traditional cross-linkers. The proposed sensor presented good selectivity for rutin and successfully applied for rutin determination in tablets with RSD of 3.05 % and the average recovery range was 98.3 %.
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