BIOSENSORS & BIOELECTRONICS
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2022
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IF 12.545
Ru Cheng,Fu Zhu,Min Huang,Qiang Zhang,Hui Hong Yan,Xiao Hui Zhao,Fu Kang Luo,Chun Mei Li,Hui Liu,Gao Lin Liang,Cheng Zhi Huang,Jian Wang
As the common hepatitis viruses , hepatitis B virus (HBV) and hepatitis C virus (HCV) are important causes of liver diseases, which greatly threaten human health and public hygiene. Furthermore, due to the similar transmission routes of HBV and HCV, it is highly susceptible to suffer from the cross-infection of HBV and HCV when humans are infected by hepatitis viruses. So it is urgent to develop strategies with high sensitivity to distinguish HBV/HCV and detect their overlapping infection. Herein, a new biosensor named “hepatitis virus indicator” for simultaneous detection of HBV and HCV based on the automatic particle enumeration was developed by the combination of Exo III assisted signal amplification strategy and particle counting technology with a dark-field microscopy. The limit of detections (LOD) were as low as 0.194 pM for HBV and 0.169 pM for HCV, respectively. In addition, the proposed method was capable of simultaneously detecting HBV and HCV based on the colorful light scattering from plasmonic nanoparticles . The proposed assay was simple, sensitive and selective for the simultaneous detection of HBV and HCV, which provided a new idea for the early diagnosis of diseases, such as hepatitis A virus (HAV), hepatitis D virus (HDV) and hepatitis E virus (HEV).
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2022
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IF 7.139
Pan Li,Xinbao Li,Ye Yuan,Yang Wang,Pengfei Shen,Xinbo Zhu,Yingying Zhu
Steam reforming of bio-oil for hydrogen production is a promising green technology. Acetic acid was used as the bio-oil model compound. Experimental and density functional theory calculations were carried out to study the performance of Co/Al 2 O 3 catalysts doped with boron (B) with a 1 wt.%–5 wt.% content. Catalyst characterization by BET, XRD , XPS , NH 3 -TPD, H 2 -TPR, TEM, and TG-DTG was performed. We found that the catalyst performance improved significantly by B doping. Under the reaction conditions of T = 500 °C, steam-to-carbon ratio (S/C) = 5, and liquid hourly space velocity (LHSV) = 4.3 h −1 , the catalyst with a B doping ratio of 1 wt.% had the highest hydrogen yield of 85% and a maximum acetic acid conversion rate of 95%. The corresponding hydrogen productivity was 0.8 mmol/min. The stability of this catalyst exceeded 29 h. Density functional theory calculations showed that the interactions between the reaction intermediates and the surface were strengthened with B addition.
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Micromachines
·
2023
·Vol. 14
·期 4
·870
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IF 3.4
Huan Wang,Mingxuan Dong,Baorui Shao,Yaodan Chi,Chao Wang,Sa Lv,Ran Duan,Boqi Wu,Xiaotian Yang
N-TiO2/Ni(OH)2nanofiber was successfully prepared by combining the electrospinning and solvothermal method. It has been found that under visible light irradiation, the as-obtained nanofiber exhibits excellent activity for the photodegradation of rhodamine B, and the average degradation rate reaches 3.1%/min−1. Further insight investigations reveal that such a high activity was mainly due to the heterostructure-induced increase in the charge transfer rate and separation efficiency.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
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2021
·Vol. 147
·期 15
·8145-8155
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IF 4.626
Tao Lili,Xu Yue,Liu Lubin,Li Bin,Xu Miaojun
A novel mono-component P/N/B-containing intumescent flame retardant poly(methylphosphonic-boric acid piperazine neopentylgcol ester) named MBPNE was synthesized. The chemical structure of MBPNE was analyzed by FTIR, 13 C and 31 P solid-state NMR tests. The results of thermal gravimetric analysis indicated that MBPNE possessed excellent charring capability, and its residual char was 27.5 mass% at 800 °C. Besides, MBPNE occurred melting and expansion process during charring evolution process. MBPNE was used to prepare flame retardant polyolefin elastomer (POE) composites. The 1.6 mm POE/MBPNE specimens achieved UL-94 V-0 rating and their LOI value was 25.5% when 22 mass% MBPNE was incorporated. The sheet was extinguished quickly after departing the flame, and no dripping appeared. Furthermore, the incorporation of MBPNE effectively enhanced the residual char mass after cone calorimeter tests, and the heat release was suppressed. The compact, expanded, partially graphitized and coherent char layer was generated on POE composites surface during burning process and exerted isolation effect in condensed phase. Meanwhile, the degradation products of MBPNE quenched active free radicals in gaseous phase, which effectually interrupted the combustion chain reaction of POE composites. Consequently, the POE composites were endowed excellent flame retardant performance.
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Bioengineered
·
2021
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IF 3.269
Bo Li,Xianyi Liu,Guogang Wu,Jiawen Liu,Shouliang Cai,Fuxin Wang,Chunyu Yang,Jisheng Liu
Colorectal cancer (CRC) is a global public health issue with increasing prevalence. MicroRNA-934 (miR-934) is a kind of non-coding RNA involved in the regulation of diverse cancers. Though previous researches have revealed part of association between miR-934 and CRC, the role of miR-934 in CRC pathogenesis has not been completely explored yet. In this study, we aim to investigate the effect of miR-934 on cell proliferation, migration, invasion and angiogenesis in CRC. Accordingly, miR-934 was found to be over-expressed in SW480 and HCT116 cells, two typical CRC cell lines. Meanwhile, miR-934 knockdown significantly inhibited cell proliferation and induced cell cycle arrest in SW480 and HCT116 cells. It was further validated that miR-934 knockdown displayed an inhibitory effect on cell migration and invasion in SW480 and HCT116 cells. Additionally, miR-934 deficiency markedly decreased VEGF expression in SW480 and HCT116 cells and suppressed capability of CRC cells to promote tube formation in vascular endothelial cells, which suggests the pro-angiogenesis role of miR-934 in vitro. Dual luciferase reporter assay further showed that miR-934 directly bound to B-cell translocation gene 2 (BTG2). BTG2 knockdown reversed the inhibitory effect of miR-934 silencing on cell proliferation, migration, invasion, and angiogenesis in SW480 and HCT116 cells. In summary, this study suggests that miR-934 facilitates CRC progression by targeting BTG2, and further highlights the role of miR-934 in pathogenesis of CRC.
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Frontiers in Pharmacology
·
2021
·
IF 5.811
Shi Hongwei,Tang Heng,Ai Wen,Zeng Qingfu,Yang Hong,Zhu Fengqing,Wei Yunjie,Feng Rui,Wen Li,Pu Peng,He Quan
Objective: Pirarubicin (THP), one of the anthracycline anticancer drugs, is widely used in the treatment of various cancers, but its cardiotoxicity cannot be ignored. Schisandrin B (SchB) has the ability to upregulate cellular antioxidant defense mechanism and promote mitochondrial function and antioxidant status. However, it has not been reported whether it can resist THP-induced cardiotoxicity. The aim of this study was to investigate the effect of SchB on THP cardiotoxicity and its mechanism.Methods: The rat model of cardiotoxicity induced by THP was established, and SchB treatment was performed at the same time. The changes of ECG, cardiac coefficient, and echocardiogram were observed. The changes of myocardial tissue morphology were observed by H&E staining. Apoptosis was detected by TUNEL. The levels of LDH, BNP, CK-MB, cTnT, SOD, and MDA in serum were measured to observe the heart damage and oxidative stress state of rats. The expression of cleaved-caspase 9, pro/cleaved-caspase 3, Bcl-2/Bax, and cytosol and mitochondrial Cyt C and Bax was evaluated by western blot. H9c2 cardiomyocytes were cocultured with THP, SchB, and mPTP inhibitor CsA to detect the production of ROS and verify the above signaling pathways. The opening of mPTP and mitochondrial swelling were detected by mPTP kit and purified mitochondrial swelling kit.Results: After 8 weeks, a series of cardiotoxicity manifestations were observed in THP rats. These adverse effects can be effectively alleviated by SchB treatment. Further studies showed that SchB had strong antioxidant and antiapoptotic abilities in THP cardiotoxicity.Conclusion: SchB has an obvious protective effect on THP-induced cardiotoxicity. The mechanism may be closely related to the protection of mitochondrial function, inhibition of mPTP opening, and alleviation of oxidative stress and apoptosis of cardiomyocytes.
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Practical Laboratory Medicine
·
2023
·
IF 1.9
Xu Tang,Lin-Ping Fan,Yang Liu
Introduction Group B streptococcus(GBS)often causes adverse outcomes such as urinary system infection, intrauterine infection, premature birth, and stillbirth in perinatal women. Perinatal screening of GBS is conducive to guiding clinical scientific intervention and improving delivery outcomes.This study quantitative real-time PCR (RT-qPCR) combined with magnetic separation was used for GBS detection. Materials and methods Sample pre-treatment in this study involved the utilization of magnetic separation (MS) technology, aiming to expedite the detection process and enhance detection sensitivity, and the cfb gene of group B streptococcus was used as the target gene to establish quantitative real-time PCR (RT-qPCR) to detect group B streptococcus. Results It was found that penicillin-functionalized magnetic beads had a good ability to enrich and capture group B Streptococcus.The findings revealed an exceptional detection sensitivity, with the ability to detect B streptococcus in urine samples at levels as low as 10 2 CFU/mL. Conclusions The utilization of MS technology in conjunction with the RT-qPCR (MS-RT-qPCR) assay, as demonstrated in this study, offers a viable approach for prenatal screening of group B streptococcus among perinatal women.
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EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Jiayi Wang,Yicen Ge,Yue Wang,Rui Sun,Xiao Yang,Haodan Xue,Xiaoyan Ma,Jinyu Liu,Keling Hu
Pd nanoparticles (NPs) supported by micelles formed with poly(2-(dimethylamino)ethyl methacrylate)- b -poly(lauryl methacrylate) (Pd/PDMAEMA- b -PLMA) were prepared and employed as catalyst in the Suzuki-Miyaura cross-coupling reaction of aryl bromides with arylboronic acids in water. Most reactions proceeded with high efficiency at room temperature with only 0.05 mol% Pd loading in the absence of ligands, which afforded the desired functionalized biaryls in 73–98 % yields after 24 h. After reaction, Pd/PDMAEMA- b -PLMA retained in the aqueous phase was easily separated from the insoluble biaryl product and could be further reused two times without significant loss in catalytic efficiency. Characterization of the catalyst disclosed that sub-2 nm Pd NPs were embedded in the hydrophilic corona of polymeric micelles that served as nanoreactors in water.
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CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Xiaoyu Pan,Bin Meng,Qingqing Yang,Xinyu Ping,Congcong Fang,Zhiyuan Ma
In order to further improve their dielectric properties of BaTiO 3 -based high-entropy ceramics, three groups of perovskite-structured high-entropy dielectric ceramics with compositions of (Ca 1/3 Sr 1/3 Ba 1/3 )(Ti 1/3 X 1/3 Y 1/3 )O 3 (X and Y chosen from Zr, Hf, and Sn) were fabricated by solid state reaction method and then fast hot-pressing sintering (FHPS) at 1300 °C and 50 MPa. All three groups of samples possess a single-phase perovskite structure with an average grain size of 160–727 nm and a uniform elemental distribution. Among the three groups of samples, (Ca 1/3 Sr 1/3 Ba 1/3 )(Ti 1/3 Sn 1/3 Hf 1/3 )O 3 exhibits the highest dielectric constant of 11,037 at the Curie temperature of 588 °C, while (Ca 1/3 Sr 1/3 Ba 1/3 )(Ti 1/3 Zr 1/3 Hf 1/3 )O 3 shows the highest discharge energy storage density ( W d = 0.702 J/cm 3 ) and the critical electric field ( CEF = 389 kV/cm) corresponding to the saturation polarization intensity. The CEF and energy storage performance of the three samples are significantly higher than those of BaTiO 3 ( W d = 0.08 J/cm 3 , η = 46.09%, CEF = 30 kV/cm) and other reported BaTiO 3 -based high-entropy ceramics.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 34
·1-10
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IF 2.8
Huang Chu,Meng Yingzhi,Li Chenlin,Wang Dawei,Chen Xue,Sun Lixian,Peng Biaolin,Lei Xiuyun,Shen Yufang,Liu Laijun
High-performance colossal permittivity ( ε′ > 10 4 ) plays an indispensable role in the development of electronic field. In this work, ultra-high dielectric permittivity ( ε′ > 237, 294 @1 kHz) and low dielectric loss (0.012 @1 kHz) were simultaneously achieved in Ta-doped BaTi 0.995 Ta 0.005 O 3 ceramics. Importantly, the dielectric permittivity changes less than 15% between − 55 and 200 °C, and the dielectric loss is less than 0.04. The excellent giant dielectric performance is related to the defect dipoles of electronic pins associated with electrons/oxygen vacancies/Ti 3+ , which are generated by B-site donor. And the thermal-activated short-range hopping of electrons is conformed to be the origin of low dielectric losses and excellent thermal stability. This work provides a strategy for achieving ultra-high dielectric permittivity, low dielectric loss, and excellent temperature stability of Ta-doped BaTiO 3 simultaneously through B-site defect engineering.
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Science Bulletin
·
2023
·
IF 18.9
Yue Zhou,Jiayuan Huang,Guanlin Wang,Zizhao Zhai,Maizbha Uddin Ahmed,Xiao Xia,Cenfeng Liu,Yuzhen Jin,Xin Pan,Ying Huang,Chuanbin Wu,Xuejuan Zhang
Pulmonary infections caused by multidrug-resistant bacteria have become a significant threat to human health. Bacterial biofilms exacerbate the persistence and recurrence of pulmonary infections, hindering the accessibility and effectiveness of antibiotics. In this study, a dry powder inhalation (DPI) consisting of polymyxin B sulfate (PMBS) inhalable microparticles and high-lectin-affinity (HLA) sugar (i.e., raffinose) carriers was developed for treating pulmonary infections and targeting bacterial lectins essential for biofilm growth. The formulated PMBS-HLA DPIs exhibited particle sizes of approximately 3 μm, and surface roughness varied according to the drug-to-carrier ratio. Formulation F5 (PMBS: raffinose = 10: 90) demonstrated the highest fine particle fraction (FPF) value (64.86%), signifying its substantially enhanced aerosol performance, potentially attributable to moderate roughness and smallest mass median aerodynamic particle size. The efficacy of PMBS-HLA DPIs in inhibiting biofilm formation and eradicating mature biofilms was significantly improved with the addition of raffinose, suggesting the effectiveness of lectin-binding strategy for combating bacterial biofilm-associated infections. In rat models with acute and chronic pulmonary infections, F 5 demonstrated superior bacterial killing and amelioration of inflammatory responses compared to spray-dried PMBS (F 0 ). In conclusion, our HLA carrier-based formulation presents considerable potential for the efficient treatment of multidrug-resistant bacterial biofilm-associated pulmonary infections.
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MICROCHEMICAL JOURNAL
·
2021
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IF 4.821
Yawen Zhang,Xia Wang,Xiang Fang,Xianxian Yuan,Huaixia Yang,Jinming Kong
As importance of early diagnosis, prognosis, and clinical monitoring in improving survival rate of lung cancer is increasing, the advance of effective diagnostic method for tumor marker is crucial. Herein, linear poly( ε -caprolactone)- b -poly(ethylene oxide) (PCL-PEO) copolymer with hydrophilic property is applied to detect CYFRA 21-1 in label-free electrochemical immunoassay. 4-(2-trimethylsilylethynyl)benzoic acid (TEB) as “bridge” bond first connects initial antibody to the surface of gold electrode. Then one side of CYFRA 21-1 antigen can recognize with initial antibody of electrode interface, and the other side hybridize specifically secondary antibody grafted PCL-PEO copolymer. So far we have constructed a complete immunoassay for CYFRA 21-1 detection using electrochemical impedance spectroscopy. The sensitivity and selectivity of analytical method depend on “Au–C” bonding, specific recognition of “antibody-antigen–antibody sandwich”, and PCL-PEO copolymer. Under optimal conditions, the analytical method for quantitative detection of CYFRA 21-1 manifests a broad linear range (1 pg/mL–10 ng/mL) and a low detection limit (0.125 pg/mL, about 3.47 × 10 -15 M). Furthermore, according to the analysis results in clinical samples, the constructed strategy also shows satisfactory sensitivity and anti-interference. It indicates that the immunoassay has broad promising applications for expecting to be applied in practical clinical prevention and monitoring.
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