PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2023
·
IF 4.7
Zhenyu Li,Mengling Chen,Wenjie Bai,Shuxia Zhang,Liwei Meng,Wei Dou,Jinjun Wang,Guorui Yuan
Carboxylesterases (CarEs) are a multifunctional superfamily of enzymes and play an important role in detoxification of various insecticides in insects. The oriental fruit fly, Bactrocera dorsalis , is one of the most destructive agricultural pests and has developed different degrees of resistance to organophosphates in field. However, the involvement of BdCarEs in tolerance or resistance to other alternative insecticides are still unclear. In the present study, 33 BdCarEs genes were identified based on the genome database of B. dorsalis . Phylogenetic analysis demonstrated that they were classified into nine clades, with abundance of α -esterases. Meanwhile, the sequence characterization and the chromosome distribution were also analyzed. The spatiotemporal expression analysis of BdCarEs genes suggested that the diversity of potential function in different physiological processes . With the exception of BdCarE21 , all BdCarEs genes responded to at least one insecticide exposure, and BdCarE20 was found to be up-regulated after exposure to all five tested insecticides individually. Eight BdCarEs genes were overexpressed in MR strain when compared to that in SS strain. Subsequently, knockdown the expression of representative BdCarEs genes significantly increased the susceptibility of the oriental fruit fly to corresponding insecticides, which indicated that the tested BdCarEs genes contributed to one or multiple insecticide detoxification. These findings provide valuable insights into the potential role in respond to tolerance or resistance to insecticides with different mode of action, and will facilitate development of efficiency management strategy for B. dorsalis .
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Yang Guo,Jianhao Lu,Zhaoqing Jin,Haorong Chen,Weikun Wang,Yaqin Huang,Anbang Wang
Lithium-sulfur batteries (LSBs) are currently being investigated as a potential candidate for high-energy–density rechargeable batteries. However, the notorious shuttle effect and uncontrolled dendrite growth posed problems for the practical applications of LSBs. Here, a new solid polymer electrolyte (SPE) was developed by in-situ polymerization of 1,3-dioxolane (DOL) in the presence of a multifunctional indium phthalocyanine (PDOL@InPc). The InPc endows the SPE with improved ionic conductivity and merit inhibition of the shuttle effect, in addition to being an effective additive for inducing uniform Li deposition by reconstructing the electric field at the interface. The characteristics of ionic migration in PDOL@InPc were quantified, and agreement between experiment and simulation established a relationship between macroscopic characteristics and molecular-level processes. The interfacial side reaction was controlled by the PDOL@InPc electrolyte, which also improved the distribution of the Li + flux for dense Li deposition. Li symmetrical batteries designed with PDOL@lnPc can withstand a current density of up to 0.5 mA cm −2 and cycle reliably for 1500 h. The PDOL@InPc electrolyte displayed a high ionic conductivity at room temperature of 3.7 mS cm −1 and a significant Li + transference number of 0.63. The resulting Li-S battery (Li|PDOL@lnPc|Sulfur) delivered a significant 325.5 Wh kg −1 of energy density at 0.2 C (1 C = 1000 mA g −1 ) at the level of the multilayer-pouch cell under an extremely low electrolyte of 3.5 g g −1 Sulfur and high loading of 8.0 mg cm −2 . This work provides a feasible strategy for the widespread implementation of LSBs with increased safety and a longer lifetime.
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MINERALS ENGINEERING
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2023
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IF 4.8
Na Chai,Lihui Gao,Shulei Li,Yijun Cao,Zilong Ma,Lingni Li,Ming Hu
In order to confirm the selectivity and mechanism of self-synthesized imprinted materials (Ga-IIP-PP) for Ga ions in mixed solutions, the adsorption experiments of bimetallic and polymetallic systems, material characterization (SEM-EDS, XPS) and Density Functional Theory (DFT) calculations were applied to examine and reveal the selective adsorption performance and mechanism of Ga-IP-PP for Ga ions. The results showed that Ga-IIP-PP exhibited excellent selectivity for Ga ions in both Ga/Al binary system and multi-ion systems, and the adsorption efficiency of Ga ions is more than 70 %. XPS analysis indicated that the phenolic hydroxyl groups in the imprinted cavity were the main adsorption sites, and the superior selectivity of the imprinted material for Ga ions derived from the stronger complexation of the imprinted sites to Ga(III), stronger electrostatic attraction of Ga-IIP-PP to Ga(III) and the high matching degree of the imprinted cavity to Ga(III). In addition, the binding energy (E ad ), Molecular Electrostatic Potential (MEP), total electron density, Mulliken charge and Frontier Molecular Orbitals (FMOs) analysis of the Ga-IIP-PP + Ga(III)/Al(III) system pointed to more electron exchange, larger absolute value of binding energy, higher electron cloud overlap, and larger orbital energy gap (E gap ) between Ga-IIP-PP and Ga ions, which confirmed a stronger adsorption affinity of Ga-IIP-PP for Ga ions than Al ions.
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FOOD AND CHEMICAL TOXICOLOGY
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2023
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IF 4.3
Xueqin Wang,Zhenzhen Cui,Yuan Luo,Yu Huang,Xinbin Yang
Xanthine oxidase (XO), a key enzyme in purine catabolism, catalyzes the oxidation of xanthine to uric acid in the body, but overproduction of uric acid may lead to hyperuricemia . This study aims to investigate in vitro XO inhibitory and in vivo anti-hyperuricemic properties of sodium kaempferol-3′-sulfonate (KS). The kinetic analysis indicates that KS is a reversible competitive inhibitor and has significant inhibitory effects on XO activity with an IC 50 value of 0.338 μM. Fluorescence spectra suggested that KS could cause fluorescence quenching and conformational changes of XO due to the formation of a KS-XO complex. Molecular docking studies demonstrated that KS interacted with several amino acid residues of XO by the π-π stacking, hydrogen bonds , and hydrophobic interactions. The inhibitory mechanism of KS on XO activity might be the insertion of KS into the active site of XO to prevent the entrance of the substrate xanthine and induce conformational changes of XO. The results carried out in hyperuricemic mice showed that KS reduced serum XO activity, serum uric acid (UA), creatinine (CRE), and urea nitrogen (BUN) levels, as well as alleviating renal histopathological injury. These findings suggest that KS may be a new potent XO inhibitor against hyperuricemia-related diseases.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
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IF 5.2
Ming Lei,Hua Huang,Junliang Liu,Feng Peng
In the demulsification process of crude oil emulsions, the temperatures of above 60 °C are most commonly required. In this work, a gemini ionic liquid (PGE-TFA) with amphipathy and interfacial activity was prepared by a simple and safe method, which could be used at the temperature of 40 °C for the effective demulsification of crude oil emulsions. The chemical structure and thermostability was investigated by FTIR, 1 HNMR and TGA . The demulsification tests of PGE-TFA showed that it had higher demulsification efficiency (DE) and faster demulsification rate than common commercial demulsifiers. PGE-TFA could be used at a low temperature of 40 °C, a wide pH range and high salinity. The DE reached as high as 95.53 % in water-in-oil emulsions with the concentration of 500 mg/L at 40 °C for 90 min. The temperature of 70 °C could slightly increase the DE and significantly improve the demulsification rate, and the DE of 97.72 % was obtained with the same concentration for 30 min. In addition, the demulsification mechanism was investigated by dynamic interfacial tension, competitive adsorption and zeta potential . The results displayed that PGE-TFA had amphipathy and interfacial activity, which promoted it quickly diffuse to the oil-water interface and displace asphaltenes at the interface. Therefore, an unstable composite film could be formed at the interface and the interfacial tension was lowered, which induced the demulsification of the emulsions.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2023
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IF 7.2
Jun Shi,Xiaoshan Zhang,Sabarinathan Ravichandran,Shirong Sun,Yulong Li,Ruoyu Wu,Wanxuan Luo,Hongmei Xing,Wenli Zhang
We report here the synthesis of N-doped porous carbon (PC) through a convenient melamine pre-grafting strategy. The melamine derivative with a monolayer structure was first grafted on the surface of the micropores of PC using a suitable diazotization reaction of melamine in an aqueous solution. After pyrolysis , PC grafting with melamine derivative was converted to the obtained N-doped PC. Meanwhile, more nitrogen dopants were grafted, and nitrogen functionalities were reconstructed to generate more graphitic N and pyridinic N atoms, in which the mesopores volume was consequently enlarged. Because of the unique structures mentioned above, the as-obtained N-doped PC exhibited enhanced oxygen reduction activity under alkaline compared with the N-doped PC from the standard physical mixture of PC and melamine. Besides, the obtained N-doped PC showed a comparable half-wave potential, remarkable durability, and good methanol tolerance compared with the commercial 20% Pt/C electrocatalyst . The results presented here could guide the development of new and improving metal-free nitrogen-doped porous carbon catalysts toward oxygen reduction reaction.
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ONCOLOGY REPORTS
·
2023
·Vol. 50
·期 2
·1-13
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IF 4.2
Xiao Wang,Simin Rong,Yunxiao Sun,Baohui Yin,Xiancong Yang,Xiaoqing Lu,Hongfang Sun,Yunfei Yan,Guangbin Sun,Yan Liang,Pingyu Wang,Shuyang Xie,Youjie Li
Imatinib resistance in chronic myelogenous leukemia (CML) is a clinical problem. The present study examined the role of N‑Myc downstream regulatory gene 3 (NDRG3) in imatinib resistance in CML. Quantitative PCR demonstrated that NDRG3 was highly expressed in patients with CML. Cell Counting Kit (CCK)‑8 experiments proved that NDRG3 promoted the proliferation of K562 CML cells and enhanced imatinib resistance. Dual‑luciferase assay showed that microRNA (miR)‑204‑5p inhibited expression of NDRG3 and immunofluorescence experiments showed that NDRG3 promoted accumulation of β‑catenin in the nucleus, thereby increasing the expression of downstream drug resistance‑ and cell cycle‑associated factors (c‑Myc and MDR1). At the same time, cell proliferation experiments showed that β‑catenin played a role in cell proliferation and drug resistance. Co‑transfection with small interfering (si)‑β‑catenin partially reversed the effect of NDRG3. This finding indicated that NDRG3 plays an important role in imatinib resistance and miR‑204‑5p and β‑catenin are involved in the biological behavior of NDRG3. The present results provide theoretical support for overcoming drug resistance in CML.
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MICROCHEMICAL JOURNAL
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2023
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IF 4.8
Mingyi Yao,Jian Liu,Narengaowa,Yonggu Li,Feng Zhao
Accurate detection of natural dyes in archaeological textiles is challenging due to limited sample availability. In situ detection of fibers by surface-enhanced Raman scattering (SERS) can overcome this challenge. In this study, we present two methods for the preparation of SERS substrates based on the laser photoreduction reaction and the galvanic displacement reaction, respectively. Our results show that the substrates prepared by these methods, including 450 nm-AgNPs, Cu-based Ag, and Zn-based Ag, offer several advantages such as rapid preparation, easy handling, low cost, high sensitivity and excellent reproducibility. Using these substrates, we successfully detected anthraquinones (alizarin, purpurin), indigoids (indigotin, indirubin), and protoberberine-type dyes in archaeological textiles from the Wei and Jin Dynasties. Notably, we were able to detect both indigo and protoberberine-type dyes in one archaeological fiber. The minimally invasive, in situ analytical approach developed provides a valuable tool for analytical applications where sampling is limited to microscopic amounts.
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FOOD CHEMISTRY
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2023
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IF 8.8
Shijin Zhou,Liang Guo,Xinheng Shi,Lele Ma,Huaixia Yang,Mingsan Miao
An electrochemical sensor based on environmentally friendly eRAFT polymerization was developed for the detection of aflatoxin B 1 (AFB 1 ) in food and herbal medicine. Two biological probes, aptamer (Ap) and antibody (Ab), were used to specifically recognize AFB 1 , and a large number of ferrocene polymers were grafted on the electrode surface by eRAFT polymerization, which greatly improved the specificity and sensitivity of the sensor. The detection limit of AFB 1 was 37.34 fg/mL. In addition, the recovery rate was 95.69% to 107.65% and the RSD was 0.84% to 4.92% by detecting 9 spiked samples. The delighted reliability of this method was verified by HPLC-FL.
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TOXICOLOGY LETTERS
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2023
·
IF 3.5
Yufen Liao,Xin Wang,Guangyun Ran,Shiyu Zhang,Chutian Wu,Rong Tan,Ying Liu,Yan He,Ting Liu,Zhongxiu Wu,Ying Peng,Weiwei Li,Jiang Zheng
Columbin (CLB) is the most abundant (>1.0%) furan-containing diterpenoid lactone in herbal medicine Tinospora sagittate (Oliv.) Gagnep. The furano-terpenoid was found to be hepatotoxic, but the exact mechanisms remain unknown. The present study demonstrated that administration of CLB at 50 mg/kg induced hepatotoxicity, DNA damage and up-regulation of PARP-1 in vivo . Exposure to CLB (10 μM) induced GSH depletion, over-production of ROS , DNA damage, up-regulation of PARP-1 and cell death in cultured mouse primary hepatocytes in vitro . Co-treatment of mouse primary hepatocytes with ketoconazole (10 μM) or glutathione ethyl ester (200 μM) attenuated the GSH depletion, over-production of ROS, DNA damage, up-regulation of PARP-1, and cell death induced by CLB, while co-exposure to L -buthionine sulfoximine (BSO, 1000 μM) intensified such adverse effects resulting from CLB exposure. These results suggest that the metabolic activation of CLB by CYP3A resulted in the depletion of GSH and increase of ROS formation. The resultant over-production of ROS subsequently disrupted the DNA integrity and up-regulated the expression of PARP-1 in response to DNA damage, and ROS-induced DNA damage was involved in the hepatotoxicity of CLB.
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HUMAN & EXPERIMENTAL TOXICOLOGY
·
2023
·
IF 2.8
Yanli Song,Dongjie He,Shaoqi Shi,Tianwei Cui,Hui Zhang,Xianmin Zhao,Tingting Ni,Huidongzi Xiao,Aiwu Wei
BackgroundWomen with antiphospholipid syndrome (APS) or antiphospholipid antibodies (aPLs) are at high risk for obstetric complications, including recurrent pregnancy loss (RPL). However, effective treatments for RPL are lacking.ObjectiveThis study aimed to reveal the function and underlying mechanism of hyperoside (Hyp) in RPL associated with antiphospholipid antibodies (aCLs).MethodsThe pregnant rats (N = 24) were divided randomly into four groups: normal human-IgG (NH-IgG); aCL-pregnancy loss (aCL-PL); aCL-PL + Hyp (40 mg/kg/day); aCL-PL + low molecular weight heparin (LMWH, 525 μg/kg/day). HTR‐8 cells were treated with 80 μg/mL aCL to establish the cell models of miscarriage.ResultsIn pregnant rats, aCL-IgG injection raised the abortion rate of embryos, while Hyp treatment inhibited the effects. Additionally, Hyp inhibited the platelet activation and uteroplacental insufficiency caused by aCL. In vivo and in vitro experiments further suggested that Hyp suppressed aCL-induced inflammation and apoptosis by downregulating NOD-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome-related factors and decreasing apoptotic rates. After aCL administration, Hyp therapy downregulated the expression of purinergic ligand-gated ion channel 7 (P2X7), which is reported to induce cytokine release and apoptosis. Furthermore, we found that the treatment of 3′-O-(4-Benzoyl) benzoyl-ATP (BzATP, an agonist of the P2X7 receptor) reversed the inhibitory effects of Hyp on cell function.ConclusionsHyp exerts protective effects on aCL-induced pregnancy loss by preventing platelet activation-mediated P2X7/NLRP3 pathway. Therefore, Hyp may provide a feasible pharmaceutical strategy for the treatment of RPL.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Zhao-Jie Wang,Yi-Chi Chen,Feng-Cai Zou,Yan Qin,Yan-Yan Zhu,Xia Xiao,Tian-Zhen Xie,Ying-Jie He,Yun-li Zhao,Xiao-Dong Luo
Zanthoxylum plants (ZPs), including multiple Chinese prickly ash species, are dual-purpose functional foods favored by the general population around the world in foods, cosmetics, and traditional medicines and have antipruritic, insecticidal, and fungicidal bioactivities. For the first time, the anti-roundworm bioactivity of ZPs and the active ingredients were compared and investigated. Through nontarget metabolomics following targeted quantitative analysis, qinbunamides, sanshools, sanshooel, asarinin, and sesamin were found to be the main different components of Zanthoxylum species. Coincidentally, the 12 chemical components were also the dominant anti-roundworm ingredients of ZP extracts. The extracts of three species of Chinese prickly ash (1 mg/mL) decreased the hatchability of roundworm eggs significantly, and the ChuanJiao seed killed roundworms (insecticidal rate 100%) and alleviated the symptoms of pneumonia in mice. Furthermore, retention time–accurate mass–tandem mass spectrometry-ion ratio (RT-AM-MS/MS-IR) were modeled by assaying 108 authentic compounds of ZP extracts, and 20 metabolites were confidently identified in biological samples from ZP extract-treated mice by analyzing the m/z values and the empirical substructures. This study provides a good reference for the proper application of ZPs.
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