Materials Science & Engineering C-Materials for Biological Applications
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2017
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IF 4.164
Lihong Qiao,Tingting Sun,Xiaohua Zheng,Min Zheng,Zhigang Xie
Targeting imaging to the desired site of action can increase the accuracy and effectiveness of diagnostic and treatment. In this work, a series of fluorescent carbon dots (CDs) were prepared by varying molar ratios of d -glucose (Glu) to l -aspartic acid (Asp). Their photophysical properties, morphologies and structures were investigated in detail. More important, the targeting ability was screened by confocal laser scanning microscopy and flow cytometry. The results indicate that CDs prepared from the optimal molar ratio of Glu/Asp (7:3) exhibit the highest targeting ability on C6 glioma cells. This work highlights the interplay of molecular design and corresponding functions, and open new possibility of developing state-of-art nanoparticles for biomedical applications.
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EUROPEAN POLYMER JOURNAL
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2018
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IF 3.741
Wu Xiaomeng,Chen Xiaoyu,Fan Zhongyong
The macroinitiator of poly(1,3-trimethylene carbonate- ran -glycolide) (P(TMC- ran -GA)) copolymers terminated with hydroxyl groups were synthesized by ring-opening polymerization (ROP) at 120 °C for 12 h. Then, the novel block copolymers consisting of P(TMC- ran -GA) segments bonding with L -lactide ( L LA) and glycolide (GA) random copolymerization segments were prepared. The poly(TMC/GA- block - L LA/GA)) block copolymers were synthesized by ROP in bulk at 130 °C with high M n ¯ (above 2.0 × 10 5 Da). Such kind of block copolymers composed of P(TMC- ran -GA) and P( L LA- ran -GA) segments show good mechanical properties and adjustable degradation rate. The molecular structures of block copolymers were characterized by GPC, FTIR, and 1 H NMR. Chain microstructure analysis was performed in detail with 13 C NMR spectroscopy. The effect of GA units content on thermal behaviors, mechanical properties as well as biodegradability of copolymers was investigated by DSC, XRD, stress-strain measurements and enzymatic degradation tested in comparison with corresponding block copolymers P(TMC- block - L LA/GA). The results shown that amorphous P(TMC- ran -GA) segments significantly improve the flexibility of block copolymers. The incorporation of GA units strongly decreases the crystallization ability of L LA segment within copolymers due to more random L LA-GA sequence and shorter average L LA block length. Meanwhile, the tensile strength of material and flexibility are significantly improved with the raising GA units content. The enzymatic degradation rate is also accelerated with the increase GA unit content. Thus, the biodegradable block copolymers with unique molecular structure and modifiable properties are promising new materials for biomedical applications.
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JOURNAL OF PLANT PHYSIOLOGY
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2025
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IF 4.1
Yijia Zhong,Zhie Zhou,Zhongping Yin,Lu Zhang,Qingfeng Zhang,Yihai Xie,Jiguang Chen
Physalis peruviana L. ( P. peruviana ) is an edible medicinal plant rich in bioactive phenolics. This study aimed to establish a hairy root (HR) culture of P. peruviana as a potential source for the synthesis of natural compounds. HRs were induced in P. peruviana using different Agrobacterium rhizogenes strains (R1601, C58C1, A4, and K599). Notably, K599 did not induce HR formation, whereas R1601, C58C1, and A4 yielded transformation frequencies of 57.78, 65.14, and 72.31%, respectively. Secondary metabolite production and antioxidant capacity were further examined in HRs induced using C58C1, R1601, and A4. It was found that A. rhizogenes R1601 induced the greatest increase (44% compared to that observed in the non-transformed culture). The methanolic extract of HRs induced by A. rhizogenes R1601 exhibited strong antioxidant capacity, with IC 50 values of 1.41 mg DE/mL and 2.33 mg DE/mL for 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), respectively. The HR culture showed higher production of phenolic compounds and higher antioxidant capacity than the non-transformed cultures. Ultra-performance liquid chromatography time-of-flight tandem mass spectrometry was used to identify eight alkaloids, phenolics, and glycoside compounds. A. rhizogenes R1601 is emerging as a possible strain for the mass production of HR and bioactive phenolic compounds in P. peruviana .
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Zhaoyu Mao,Xiang Gao,Juan Qiu,Chunchao Chen,Kangwei Gongsun,Changming Bu,Qing Zang,Min Yu,Houyi Ma
A simple two-electrode system was utilized to fabricate a layer of CuCl on the surface of bronze for simulating the "bronze disease". The addition of L-arginine (Arg) reduced the conversion time of the CuCl patina in water from 6 h to 3 h and enhanced the protection efficiency of the transformed patina from 27.9 % to 81.8 %. The alkalinity of Arg and its coordination capacity with Cu 2 + facilitate the transformation of CuCl, modify the transformation mechanism, and alter the morphology and structure of the patina. The immersion experiment further substantiates the stability and self-reinforcing properties of the Arg-transformed patina.
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ACS Sustainable Chemistry & Engineering
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2024
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IF 7.1
Meng-Qi Wang,Ming-Xin Sun,Han-Guang Li,Ze-Hua Wu,Jian-Zhong Xu
l-Hypaphorine (l-HYP) is an l-tryptophan-derived alkaloid with significant medicinal value. The objective of this study tried to use Escherichia coli as a chassis cell to produce l-HYP from glucose by fermentation. To do this, the optimized methyltransferases were first mined based on the data of the phylogenetic tree of methyltransferase, indicating that the methyltransferase from Mycobacterium smegmatis with two mutation sites (i.e., EgtDM252 V,E282A and MsE) was designed as the best enzyme for producing l-HYP using l-tryptophan as a substrate. After this, a mutant MsET163G with high catalytic efficiency was constructed by semirational directed evolution focusing on the substrate binding cavity. To achieve the self-supply of l-tryptophan (l-Trp) and S-adenosyl methionine (SAM) in E. coli, the biosynthetic pathway of l-Trp and SAM was reconstructed. As a result, the final strain E. coli HYP-12 could produce 200.5 ± 2.6 mg/L of l-HYP with a glucose conversion efficiency (α) of 2.01% in shake-flask fermentation. This is the first report on producing l-HYP from glucose by microbial fermentation, which provides a real possibility for industrial l-HYP production using environmentally friendly methods rather than extraction from plant and provides a general concept for constructing other nonendogenous products’ high-producing strains.
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Foods
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2024
·Vol. 13
·期 22
·3691
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IF 4.7
Haicui Suo,Jitao Liu,Li Wang,Chengchen Li,Jianwei Shan,Kang An,Kun Yang,Xiaobo Li
Individual carotenoids provide significant health benefits to humans, and potatoes are recognized as some of the most stable crops, distinguished by their substantial carotenoid content. To elucidate the accumulation patterns of individual carotenoids in potatoes, we quantified the carotenoid content in yellow- and white-fleshed genotypes across five developmental stages using LC-MS/MS. A total of 22 carotenoids were identified in yellow potatoes, whereas 18 were detected in white potatoes. The yellow-fleshed genotype was characterized by high levels of individual carotenoids and xanthophyll esters, with violaxanthin as the dominant component. The white-fleshed genotype exhibited low concentrations of individual carotenoids and xanthophyll esters, with lutein as the predominant compound. Notably, lutein, violaxanthin, zeaxanthin, antheraxanthin, neoxanthin, violaxanthin myristate, and lutein myristate were consistently detected during the developmental period in both genotypes. Violaxanthin myristate was identified as the dominant xanthophyll ester in both genotypes, showing an increasing trend throughout the tuber development stages. In contrast, xanthophyll esters maintained stable low levels in white-fleshed potatoes while exhibiting increasing types and contents in yellow-fleshed potatoes as the tubers continued to grow. Additionally, violaxanthin exhibited a significant correlation with b*, H°, and C*, suggesting that it may play an important role in forming yellow flesh.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Xin Sun,Yuchan Meng,Keling Hu,Jinming Sun,Chunyu Zhou,Chengkun Su,Lihui Zhang,Chunhong Zhang,Zhengfeng Ma
The poly(L-lactic acid) (PLLA)/ZnO composite membrane with cluster assemblies microstructure was constructed by a combination of non-solvent induced phase separation (NIPS) and the Breath-Figure method. In this novel method, the controllable diffusion rate between solvent and non-solvent was introduced to the system by adjusting the non-solvent solubility parameters. The humidity was adjusted to control non-solvent solubility parameters in the Breath-Figure method, which avoids the instantaneous phase separation induced by direct coagulation of water droplets. Hydrophobic modified ZnO nanoparticles were used as heterogeneous nucleation points to induce PLLA crystallization and formation of micro-nano structures. Controlling molecular chain growth with crystal nuclei as templates and constructing cluster assemblies microscopic morphology at 99 % humidity, and the size of the cluster decreases gradually from 10 μm to 3 μm as the nanoparticles content increased up to 5 wt%. The surface water contact angle could reach 153.8° with cluster morphology. In addition, the porous structure formed by the polymer-lean phase could increase the porosity to 93.1 % and exhibit an excellent oil absorption capacity up to 12.64 g/g. It is foreseeable that porous PLLA/ZnO composite membranes have potential applications as biodegradable oil-water separation materials.
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Sugar Tech
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2025
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IF 2
He Wentao,Liu Naixin,Zhou Qin,Li Linxuan,Zhao Wenwen
There is a lack of systematic analysis and research on the special components of red beet and sugar beet, which are common beet varieties rich in a variety of beneficial chemicals. To investigate the differences in the composition of flavonoids and terpenoids in roots of red beet and sugar beet, poinsettia(red beet) and HD802(sugar beet) were detected and analysed in the present study by using the UPLC-MS/MS detection platform and the MWDB(Metware database), and orthogonal partial least squares discriminant analysis (OPLS-DA) was used to screen out the differential metabolites from the detected flavonoid metabolites and terpenoids metabolites. A total of 69 flavonoid metabolites from 6 classes and 48 terpenoids from 6 classes were detected in two kinds of beet roots. Analysis of variance (ANOVA) showed that the content of 5 flavonoid and 4 terpenoid differential metabolites was higher in red beet roots than in sugar beet roots, and the content of the remaining 25 flavonoid and 2 terpenoid differential metabolites was lower than that of sugar beet roots. According to the metabolite Log 2 FC, the components of the flavonoid metabolites with large difference ratio were mainly Polymethyl flavonoid derivatives, Iso-rhamnoside and Rhamnoside, Marigold glycoside, Methoxyl flavonoid derivatives, Diosmetin derivatives, Chrysoeriol and its derivatives, Luteolin derivatives, Homonetsin derivatives and Kaempferol derivatives. Significant differences in flavonoid metabolites and terpenoid metabolites between the two beet roots were found by non-targeted analysis of variance and can be further analysed as characteristic metabolites.
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FUEL
·
2024
·
IF 6.7
Renjie Ji,Defu Yin,Yao Chen,Like Ma,Fanglin Lv,Yuhao Zhang,Zhen Jia,Jingyun Zhang,Mingya Liu,Shitao Yu,Yue Liu
A core–shell catalyst mZSM-5@SO 4 2- /mSiO 2 composed of mZSM-5 core and SO 4 2- /mSiO 2 shell was prepared by the soft template method. Core-shell structure could promote the building of Brønsted -Lewis (B-L) acids interface and significantly improve the synergistic effect of B-L. In this paper, the Brønsted/Lewis (B/L) of mZSM-5@SO 4 2- /mSiO 2 was targeted regulating by innovatively introducing Brønsted acid (BA) sites into the shell. The optimal mZSM-5@SO 4 2- /mSiO 2 catalyst (B/L = 0.142) could achieve 72.1 % furfural yield at 140 ℃ for 1 h. In addition, 70.2 % furfural yield could be obtained after the catalyst was recycled and regenerated for 5 times. Combining In-situ IR and 13 C NMR, the reaction path was revealed in detail: xylose was first isomerized to xylulose and then dehydrated to furfural. This paper provides a research idea for the directional regulation of dual active sites of cascade reaction catalysts.
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FOOD CHEMISTRY
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2024
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IF 8.5
Cong Liu,Ying Zou,Manli Zhang,Chaoxian Chi,Di Zhang,Fangling Wu,Chuan-Fan Ding
This work presents a straightforward approach to the separation d / l -carnitine ( d / l -Carn) using ion mobility–mass spectrometry (IM-MS) and theoretical calculations. Natamycin (Nat) was used as separation reagent to interact with the Carn, metal ions (G) were employed as ligand, the resultant ternary complexes [ d / l -Carn + Nat + G] + were observed experimentally. IM-MS results revealed that d / l -Carn could be baseline separated via complex formation using Li + , Na + , K + , Rb + , and Cs + , with a maximum peak separation resolution (R p-p ) of 2.91; Theoretical calculations were performed to determine the optimal conformations of [ d / l -Carn + Nat + Li/K] + , and the predicted collisional cross section values were consistent with the experimental values. Conformational analysis was used to elucidate the enantiomeric separation of d / l -Carn at the molecular level via the formation of ternary complexes. Furthermore, quantitative analyses for the determination of the enantiomers were established with effective linearity and acceptable sensitivity. Finally, the proposed method was successfully applied in the determination of d / l -Carn in food samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Sihui Xiong,Ming Chen,Xun Pei,Jinjing Yin,Hélder Oliveira,Nuno Mateus,Shuxin Ye,Muci Wu,Rui Zhang,Jingren He
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
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FOOD CHEMISTRY
·
2024
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IF 8.5
Li Zhang,Chao Li,Yue Chen,Shaobin Li,Xue Wang,Fengbo Li
L-tryptophan (L-Trp) is crucial for human metabolism, and its imbalance or deficiency can lead to certain diseases, such as insomnia, depression, and heart disease. Since the body cannot synthesize L-Trp and must obtain it from external sources, accurately monitoring L-Trp levels in food is essential. Herein, a nanocomposite film based on polyoxometalate (P 2 Mo 17 V), Ti 3 C 2 T x MXene , and chitosan (Cs) was developed through a green electrostatically mediated layer-by-layer self-assembly strategy for electrochemical detection of L-Trp. The composite film exhibits fast electron transfer and remarkable electrocatalytic performance for L-Trp with a wide linear range (0.1–103 μM), low limit of detection (0.08 μM, S/N = 3), good selectivity, reproducibility, and repeatability. In milk sample, the recoveries of L-Trp were from 95.78% and 104.31%. The P 2 Mo 17 V/Cs-Ti 3 C 2 T x electrochemical sensor not only provides exceptional recognition and detection capabilities for L-Trp but also shows significant potential for practical applications, particularly in food safety and quality control .
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