Nature Protocols
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2026
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IF 18.4
Zuo Fangting,Su Ni,Xie Xin,Jiang Li,Fang Mengyue,Zhao Yuzheng,Zhu Linyong,Chen Xianjun,Yang Yi
RNAs exhibit complex dynamics in cells, including expression, splicing, localization, translation and degradation, and these processes are highly coordinated and tightly regulated both spatially and temporally. To better understand the biological function of diverse RNAs, approaches that allow monitoring of RNA with high spatiotemporal resolution are essential. Fluorescent RNAs (FRs), fluorescent protein–like entities consisting of RNA aptamers and their cognate fluorogenic dyes, have emerged as a promising approach for imaging RNA dynamics in live cells. We recently reported the development of several high-performance FRs, named Pepper, Clivia and Okra, that show advantageous properties, including high cellular brightness and photostability, low ion dependence and/or large Stokes shifts, and have been used to image diverse RNA species in live cells. In this protocol, we provide easy, efficient and generalizable strategies for using FRs to visualize different RNA species in bacteria and mammalian cells by expressing the RNA of interest tagged with one or more copies of the aptamer. We also provide a detailed procedure for multiplexed RNA imaging using orthogonal FRs and the steps to perform super-resolution live imaging of RNAs. The protocol typically takes 5–7 d, including cloning, transfection of mammalian cells or transformation of bacteria, live imaging and results analysis. This protocol is applicable to the real-time monitoring of the localization and dynamics of RNAs of interest in live cells.
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Nature Communications
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2026
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IF 18.1
Wang Xiaoyu,Cai Zhaohui,Chen Zeping,Liu Donliang,Chen Wanqing,Zhu Jianqiu,Li Wenhuai,Wang Xixi,Zhang Linjuan,Wang Wei,Zhou Chuan,Zhou Wei,Shao Zongping
The key challenges for commercializing reversible proton ceramic electrochemical cells (R-PCECs) are the insufficient proton conductivity and inferior thermomechanical stability of oxygen electrodes in air with water vapor. We report a multielement micro-doped BaCoO 3-δ -based perovskite material, in which disorder is induced in the ionic substructure to maximize the oxygen-water reaction activity. Atom probe tomography and density functional theory calculations reveal that reduced proton adsorption/diffusion energy barriers are triggered by homogeneous ion distributions in the perovskite oxide. Moreover, the thermally driven mild oxygen release can be further offset by beneficial proton uptake, thereby increasing the thermomechanical durability of the oxygen electrode. The resulting R-PCECs obtain a peak power density of 1.56 W cm -2 and an electrolysis current density of 2.0 A cm -2 @1.3 V at 600 °C while demonstrating long-term stability exceeding 780 hours, with degradation rates of 19.3 and 16.9 μV h -1 in fuel cell and electrolysis modes, respectively.
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Nature Communications
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2026
·Vol. 17
·期 1
·2500
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IF 18.1
Li Minghang,Fu Zhaoyuan,Luo Qian,Lu Bing,Zhang Liwei,Liang Shipan,Liu Ting,Wang Yong,Mao Shanjun
The entangled electronic and geometric effects in heterogeneous catalysis have long obscured precise attribution of their individual roles. By employing a thermal-induced surface reconstruction strategy to progressively enhance metal-support interactions, we achieve continuous tuning of Pd’s electronic and geometric structures. Using alkyne semi-hydrogenation as a prototypical structure-sensitive probe reaction, we demonstrate that despite the linear coupling of electronic and geometric structures during Pd reconstruction, the turnover frequency scales solely with the geometric descriptor W (quantifying Pd particle flattening), while selectivity regulation bifurcates into two distinct regimes: Below a critical W, electron-deficient Pd sites offset geometric constraints, yielding a non-structure-sensitive selectivity plateau; whereas above this threshold, electron-rich surfaces establish unequivocal geometric control. Moreover, surface reconstruction drastically shrinks the electronic-geometric space of Pd sites for over-hydrogenation side reaction. These insights provide a blueprint for metal catalysts regulation in hydrogenation and may open avenues for quantifying electronic-geometric interplay in other structure-sensitive reactions.
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Scientific Reports
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2026
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IF 4.9
Chen Weihua,He Rui,Li Li,Bai Jiejing,Li Zhengyong,Wang Tao,Luo Le,Zhang Xinyu,Zhang Wei
Tight sandstone reservoirs are characterized by low porosity and low permeability, which results in great difficulty in oil production and low recovery. In this work, based on fracture–matrix tight sandstone core models and field crude oil, the interfacial activity and reservoir adaptability of the oil displacement agent C-22 were evaluated. Subsequently, oil displacement agents with different interfacial tension levels were optimized and selected as control groups for subsequent oil displacement experiments. The migration behavior of crude oil in fracture–matrix cores during spontaneous imbibition and oil displacement processes using C-22 was systematically investigated, and the key development parameters for oil displacement were further optimized. The results show that C-22 exhibits excellent interfacial activity and good reservoir adaptability. An ultralow interfacial tension of 0.12 mN/m can be achieved at a concentration of 0.1 wt%, and the interfacial tension remains stable after 7 d of aging. In the oil displacement experiments, as the pressure decreases from 15 to 0 MPa, the final oil recovery reaches 18.94%. Nuclear magnetic resonance analysis indicates that oil in mesopores and macropores is predominantly mobilized at the early stage, whereas oil in micropores is mainly produced at the later stage. Furthermore, the key development parameters for the oil displacement process were optimized. The optimal oil displacement performance is achieved when the interfacial tension is reduced to the 10 −2 mN/m level, the concentration of C-22 is 0.2 wt%, and the shut-in time is 12 h. We expect that this study can provide valuable insights into the effective development of tight sandstone reservoirs and offer theoretical guidance for the selection of field operational parameters.
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BIOGERONTOLOGY
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2026
·Vol. 27
·期 2
·72
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IF 5.3
Zhang Xiaoli,Wang Chengniu,Chen Weiguan
Aging is a major risk factor for the onset and progression of many neurodegenerative diseases. Inflammation is the body’s natural defense mechanism against harmful stimuli . A growing body of research has highlighted the intricate relationship between aging and inflammation, with chronic low - grade inflammation, often referred to as “inflammaging,” being a hallmark of the aging process. Cycloneolitsol (CL) is a C-32 cycloartane-type triterpene from Taxodium ascendens which has been reported to possess the anticancer, neuroprotection and antibacterial activities. Its specific role and the underlying molecular mechanisms in modulating the aging process remain unclear. Our study aimed to explore the possible anti-aging effect and mechanisms of CL. Network pharmacology predicted core targets and several potential pathways, which are associated with the anti-aging effect of CL. RAW264.7 macrophages and Caenorhabditis elegans were selected for evaluating its anti-aging and anti-inflammation activity. The results showed that CL exerted obvious anti-inflammatory effects against LPS-induced M1-type polarization of RAW264.7 cells via NF-κB signaling pathway. In C. elegans , CL improved Biological Characteristics and prolonged the lifespan without reproductive toxicity. Furthermore, experiments on different mutant C. elegans strains and the nuclear translocation of SKN-1 confirmed that CL activates autophagy and enhances the antioxidant stress response ability through the SEK-1/PMK-1/SKN-1 signaling pathway. This study provides novel insights into the biological activities of CL, highlighting its potential as a natural product for treating inflammation-related diseases and delay aging.
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CATALYSIS LETTERS
·
2026
·Vol. 156
·期 4
·101
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IF 2.5
Zou Congyang,Liu Ruijie,Yang Mingjie,Tian Jie,Wang Yujie
Exploring stable and efficient photocatalysts to promote the photo-oxidative degradation of volatile organic compounds (VOCs) is essential for air pollution control. In this study, MIL-101(Fe) modified with silver nanoparticles (Ag NPs) was synthesized via a simple solvothermal method. Under visible light irradiation for 90 min, the optimal catalyst MF/Ag-5% composite achieved a styrene conversion rate of 91.8%, with a corresponding kinetic rate constant of 0.022 min⁻ 1 . Moreover, the catalyst retained its photocatalytic activity over four consecutive cycles. The results indicate that the incorporated Ag NPs effectively extract photogenerated electrons from MIL-101(Fe), thereby promoting efficient charge carrier separation. Additionally, the localized surface plasmon resonance (SPR) effect of Ag NPs extends the light absorption range of MIL-101(Fe), facilitating the generation of reactive species such as hydroxyl radicals (·OH) and superoxide anion radicals (·O₂⁻). This work offers a novel approach for designing highly efficient and stable photocatalysts for air purification applications.
Section
Graphical
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CELLULAR AND MOLECULAR LIFE SCIENCES
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2026
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IF 6.5
Huang Yafei,Liu Hong,Tong Chao,Wang Zhimin,Xu Miaomiao,Dong Mengqi,Rao Rongjia,Wang Xianqiang,Feng Wei,Hu Zhan,Xu Fei,Zhao Wei,Wang Li,Hu Shengshou,Zhou Bingying
Drug-induced mitochondrial toxicity is a major contributing factor to cardiotoxicity, which can cause drug attrition and adverse cardiac events. To assess the toxicity of anti-inflammatory agents, we used adult human primary cardiomyocytes (hPCMs) to screen 18 clinically available anti-inflammatory drugs in a high-content manner, and revealed widespread mitochondrial dysfunction without affecting cell viability. Nabumetone, a representative nonsteroidal anti-inflammatory drug with profound mitochondrial toxicity, induced mitochondrial fission, inhibited mitophagy, and impaired both electrophysiological and metabolic functions in adult hPCMs. Mechanistically, we uncovered that nabumetone (Nab) exerted its toxic effects through the prostaglandin E2- E-type prostanoid receptor 4 (PGE 2 -EP 4 ) pathway, which was essential for its anti-inflammatory functions. To find an alternative route to ameliorate mitochondrial damage, we identified SIRT3 as a downstream target of nabumetone. Its mRNA, protein, and activity levels were significantly reduced upon nabumetone treatment. SIRT3 activator honokiol exhibited protective potential against NSAID-induced mitochondrial toxicity both in hPCMs and in nabumetone-treated mice. Finally, through screening mitochondrial liability in various common cardiomyocyte models, we identified mitochondrial abundance as an important determinant of the sensitivity of cells towards mitochondrial toxicants. Our study demonstrates the vast presence of mitochondrial dysfunction in human adult cardiomyocytes imposed by clinically used anti-inflammatory drugs, and identified both toxicity and protective pathways that may serve future therapeutic purposes.
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JOURNAL OF CHEMICAL SCIENCES
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2026
·Vol. 138
·期 1
·15
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IF 1.8
Wang Kun-Peng,Liu Jie,Chen Xi,Wu Gui-Lin,Zhang Er-Ji,Deng Tian-Song
Gold nanorods, as a type of anisotropic nanoparticles, require uniformity for their properties and applications. Here, we demonstrate the successful preparation of well-dispersed and uniformly shaped GNRs through a two-step seed-mediated growth method by separating the symmetry breaking of seeds from subsequent growth. We determined that adding the first growth solution (Solution A) into the second growth solution (Solution B) after 30 min was optimal, and monitored the growth progress by recording the growth status every 30 min, observing that the growth of Solution A (150 min) and Solution B (210 min) essentially ceased. Furthermore, by adjusting the seed quantity, silver ion content, and pH value of the growth solution in each step, we could tune the wavelength of the localized surface plasmon resonance (LSPR) peak within the range of 668 to 1020 nm. Additionally, the trend of overall electric field intensity variations were determined through finite difference time domain (FDTD) simulations. We believe that this approach will further facilitate practical applications of GNRs research, as these applications rely on the high uniformity of GNRs. Graphical abstract Synopsis .
We demonstrate the preparation of uniformly shaped gold nanorods through a two-step seed-mediated growth method by separating
the symmetry breaking of seeds from subsequent growth. By adjusting the seed quantity, Ag + content, and pH value of the growth
solution, we could tune the LSPR from 668 to 1020 nm.
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Materials for Renewable and Sustainable Energy
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2026
·Vol. 15
·期 1
·6
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IF 5.5
Li Ying,Wu Min Cong,Zhang Xin
This study presents a systematic investigation of CeO 2 -doped reduced graphene oxide (RGO) as a support for platinum catalysts in direct methanol fuel cells. CeO 2 /RGO composites with loadings of 3–10 wt% CeO 2 were synthesized via controlled precipitation and thermal reduction, followed by the deposition of Pt nanoparticles using ascorbic acid as a reducing agent. Physical characterization by XRD, TEM, and XPS confirmed the uniform dispersion of Pt nanoparticles (3–4 nm) and revealed chemical interactions between Pt and CeO 2 . Electrochemical testing demonstrated that the catalyst with 5% CeO 2 doping exhibited optimal performance: A methanol oxidation current of 959 mA/mgPt, which is four times higher than that of the undoped Pt/RGO benchmark. An electrochemical surface area (ECSA) of 87.1 m 2 /g. Enhanced CO tolerance, as indicated by an If/Ib ratio of 1.24. The enhanced catalytic activity is attributed to the oxygen storage capacity of CeO 2 and synergistic interfacial effects between Pt and CeO 2 , which were evidenced by a positive shift in the Pt4f binding energy observed in XPS analysis. This work demonstrates a cost-effective strategy for improving Pt utilization in fuel cell anodes through doping with rare earth oxides.
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JOURNAL OF MATERIALS SCIENCE
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2026
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IF 4.4
Chen Lan,Zhang Xi-Hao,Ito Hironori,Deng Tian-Song
Gold nanobipyramids (AuNBPs) possess sharp tips and highly tunable near-infrared localized surface plasmon resonance (LSPR) modes, making them attractive building blocks for plasmonic superstructures. However, achieving controllable and stable one-dimensional (1D) self-assembly in aqueous phase remains challenging. Here, we present an interface-mediated strategy in which CTAB regulates tip accessibility, while 4,4′-bipyridine (4,4′-BPy) participates in coordination-assisted interactions that modulate the assembly pathway and bias AuNBPs toward end-to-end configurations. Controlled CTAB desorption selectively exposes the {111} tip sites, enabling ligand-assisted interactions that favor anisotropic chain growth. Subsequent CTAB re-passivation enables kinetic stabilization of the assemblies, allowing chain elongation to be terminated at selected stages and yielding plasmonic nanochains with good long-term structural stability. Together, this synergistic combination of interfacial regulation, coordination-assisted interactions, and kinetic stabilization provides a versatile framework for constructing stable near-infrared plasmonic architectures and offers general design principles for manipulating collective optical modes in anisotropic nanostructure assemblies.
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JOM
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2026
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IF 2.3
Jia Boshuai,Wang Zhanli,Wang Liping,Li Baoe,Liu Shimin,Yang Tai,Wang Donghui,Liang Chunyong,Wang Hongshui
The infections associated with dental and orthopedic implants always result from the biofilm of bacterial colonization, leading to implant failure, prolonged hospitalization, and increased healthcare costs. Iron oxide (Fe 3 O 4 ) nanoparticles and indocyanine green (ICG) are both reported to have good antibacterial properties. However, the anti-biofilm efficiency of either component alone is still unsatisfactory. In this paper, octahedral Fe 3 O 4 nanoparticles (O-Fe 3 O 4 ) were synthesized and integrated with ICG to achieve the best biofilm removal effect, and the optimized ratio between the Fe 3 O 4 and ICG was also investigated. The results showed that ICG inhibited the magnetic properties of Fe 3 O 4 slightly (from 92 emu/g to 76 emu/g); however, it endowed Fe 3 O 4 with significantly improved photothermal, photodynamic, and peroxidase-like activities, achieving excellent antibacterial property (eradicating more than 99% of S. aureus under the combination of H 2 O 2 treatment and laser irradiation). Combined with the good cytocompatibility (cell viability > 80% at a concentration of 250 μg/mL), the synthesized O-Fe 3 O 4 @ICG nanoparticles (especially O-Fe 3 O 4 @ICG-10) have strong application potential for synergistic therapy against biofilm infection associated with dental and orthopedic implants. This study presents a promising nano-antibacterial system for combating resistant biofilm infections, offering insights into the rational design of synergistic nanotherapeutics for clinical applications.
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Journal of Inorganic and Organometallic Polymers and Materials
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2026
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IF 6
Liu Xiaoju,Ye Zeyu,Tang Qiufan,Ma Xiaoyan,Wang Guoxiong,Chen Zhou
To develop high-efficiency catalysts for solid propellants, this study synthesized monometallic (FcHz-Co) and bimetallic (FcHz-CoNi, FcHz-CoPb) MOFs using a ferrocene-based ligand (FcHz). Ferrocene derivatives are classic combustion promoters for solid propellants, while MOFs optimize ferrocene dispersion and enrich active sites-key to enhancing catalytic performance. Infrared spectroscopy, SEM, and XPS characterizations showed FcHz forms stable MOFs via N (from -NH₂/C = N groups)-metal coordination, while the ferrocene moiety, core combustion-promoting group, remains intact. Metal electron affinity (EA) is the core factor regulating material properties: FcHz-CoNi (Co: 0.662 eV, Ni: 1.157 eV) has well-dispersed spherical morphology (50.1 m²/g specific surface area) and balanced electron distribution due to bimetallic EA synergy; FcHz-Co (Co: 0.662 eV) has cubic morphology with secondary performance (limited by single-metal EA); FcHz-CoPb (Pb: 0.356 eV) agglomerates (11.2 m²/g specific surface area) due to low EA and large Pb²⁺ radius, showing the poorest performance. Catalytic tests confirmed FcHz-CoNi optimally promotes AP thermal decomposition: it reduces AP’s high-temperature decomposition peak from 441 ℃ to 329 ℃, boosts heat release to 1887 J/g, and lowers apparent activation energy to 89.49 kJ/mol. This study provides support for new propellant catalyst design and proposes a targeted strategy centered on “metal EA matching”.
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