Food Bioscience
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2024
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IF 4.8
Zeyu Wu,Jiting Gao,Chongwei Yu,Wei Zhao,Nan Chen,Teresa G. Valencak,Daxi Ren
Diabetes mellitus is a chronic metabolic disease characterized by hyperglycemia, with its prevalence escalating. It exerts a profound impact on human health care and socio-economic development. Probiotics, known as natural, safe, and minimal side effects, have emerged as promising intervention agents to manage chronic diabetes. Our study aimed to explore the therapeutic potential of Lactobacillus rhamnosus ZJUIDS07 for mitigating T2D in mice induced by a high-fat diet (HFD) and streptozotocin (STZ). Administration of L. rhamnosus ZJUIDS07 led to a reduction in diabetic symptoms, including reversal of weight loss, improvement in hyperglycemia and insulin resistance, mitigation of disruptions in glucose and lipid metabolism, alleviation of pancreatic pathological damage, and reduction in oxidative stress and inflammation. Moreover, treatment with L. rhamnosus ZJUIDS07 could restore intestinal microbiota balance in T2D mice by altering the structure and composition of intestinal microbiota, and increased short-chain fatty acids (SCFAs) levels. Specifically, L. rhamnosus ZJUIDS07 significantly decreased the abundance of harmful bacteria like Staphylococcus , Corynebacterium , and Odoribacte , while increasing the abundance of beneficial bacteria (especially SCFAs-producing bacteria) such as Muribaculaceae_norank, Dubosiella , Clostridium sensu stricto 1, Lactobacillus , and Faecalibaculum . Our findings highlight that L. rhamnosus ZJUIDS07 represents a promising probiotic strategy for future treatment of T2D, and that the modulation of gut microbiota and their metabolites through the microbiome-gut-pancreas axis play a pivotal role for the antidiabetic effects of L. rhamnosus ZJUIDS07.
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WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2025
·Vol. 41
·期 4
·1-15
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IF 4.2
Zhao Jing-yi,Gao Fan,Wu Mengru,Li Yang,Chen Yong,Xiao Zijun
Enantiopure ( S )-2-methylbutanoic acid [( S )-2-MBA] is a high-value chiral compound with applications in fragrances, pharmaceuticals, and agrochemicals. However, conventional chemical synthesis lacks stereoselectivity, while existing biosynthetic methods suffer from low yield and purity. Here, we report a novel microbial process using Bacillus spizizenii ATCC 6633 for efficient ( S )-2-MBA production via L-isoleucine catabolism. Through targeted screening of rhizospheric soil isolates and Bacillaceae strains, ATCC 6633 demonstrated superior performance, producing 3.67 g/L ( S )-2-MBA with 99.32% enantiomeric excess ( ee ) under optimized conditions (45 °C, 8% inoculation, 5 g/L glucose, and 8 g/L L-isoleucine). A 58.92% conversion efficiency was achieved, and a simplified purification process recovered 63.90% product with 97.32% purity. Mechanistic studies suggested glucose depletion triggered ( S )-2-MBA accumulation, aligning with starvation-induced secondary metabolism. This cost-effective, eco-friendly approach eliminates racemic separation steps and harsh reagents, positioning ATCC 6633 as a promising biocatalyst for sustainable ( S )-2-MBA production.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
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IF 8.1
Qi Cao,Yucheng Shen,Jixing Bai,Qingyu Liu,Zhenzhen Zhao,Tingzhou Lei,Abdelghaffar S. Dhmees,Huiyan Zhang
Synthesizing high performance and affordable nickel-based electrocatalysts has been one of the research hotspots in the field of clean energy conversion. Herein, facile electroless plating accompanied with mild impregnation method is explored to synthesize petal-like Ni(OH) 2 flakes on carbon cloth (CC) for electrocatalytic alkaline hydrogen evolution reaction (HER). Notably, the optimal Ni(OH) 2 /CC electrode has outstanding HER activity requiring only 24.6 mV vs . RHE of overpotential to reach 25 mA cm −2 of current density, and only 101.5 mV vs . RHE of overpotential to reach 100 mA cm −2 . Meanwhile, the 25-h stability test results performed well. This is primarily due to its plentiful interfacial contact, favorable surface morphology of ultrathin and dense nanosheets array, and rapid electron transport . Overall, this study provides a viable approach for the development of efficient, low-cost, hydrophilic nickel-based catalysts for electrocatalytic water splitting applications.
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CARBOHYDRATE POLYMERS
·
2025
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IF 12.5
Qijun Yu,Jinshui Yang,Liang Liu,Yaru Huang,Entao Wang,Dongmei Li,Hongli Yuan
Enzyme immobilization technology holds significant value in biocatalysis. Carbohydrate-binding modules (CBMs), with their specific binding to natural polysaccharides, offer a highly promising immobilization method. In the present study, the binding ability with their natural substrates and heterologous expression levels of four CBMs using fluorescent protein tagging were studied, revealing that CBM2r presented the highest immobilization efficiency and expression level. Using the Design of Experiments (DOE), the immobilization conditions for mCherry-CBM2r were optimized, achieving a protein loading of 2.45 wt% on Avicel under optimal conditions: a solid-liquid ratio of 1:30, NaCl concentration of 108 mM, protein concentration of 6 mg/mL, and incubation time of 120 min. Subsequently, CBM2r gene was fused with chitosanase gene from Bacillus subtilis (BsCsn) and expressed in Escherichia coli for establishing a novel one-step immobilization of fusion enzymes mediated by CBM2r on microcrystalline cellulose. The immobilized CBM2r-BsCsn-Avicel was used for batch hydrolysis of high-concentration chitosan to produce chito-oligosaccharides, with the enzyme retaining 96 % substrate degradation efficiency over seven cycles and achieving a space-time yield of 232.8 kg/m 3 /h. This study provides a simple, cost-effective, environment friendly, and competitive biocatalytic immobilization strategy.
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Cell Reports Medicine
·
2024
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IF 11.7
Chao Zhang,Yu-Xuan Sun,Ding-Cheng Yi,Ben-Yuan Jiang,Li-Xu Yan,Ze-Dao Liu,Li-Shan Peng,Wen-Jie Zhang,Hao Sun,Zhi-Yong Chen,Dan-Hua Wang,Di Peng,Song-An Chen,Si-Qi Li,Ze Zhang,Xiao-Yue Tan,Jie Yang,Zhang-Yi Zhao,Wan-Ting Zhang,Jian Su,Yang-Si Li,Ri-Qiang Liao,Song Dong,Chong-Rui Xu,Qing Zhou,Xue-Ning Yang,Yi-Long Wu,Ze-Min Zhang,Wen-Zhao Zhong
The clinical efficacy of neoadjuvant immunotherapy plus chemotherapy remains elusive in localized epidermal growth factor receptor (EGFR)-mutant non-small cell lung cancer (NSCLC). Here, we report interim results of a Simon’s two-stage design, phase 2 trial using neoadjuvant sintilimab with carboplatin and nab-paclitaxel in resectable EGFR-mutant NSCLC. All 18 patients undergo radical surgery, with one patient experiencing surgery delay. Fourteen patients exhibit confirmed radiological response, with 44% achieving major pathological response (MPR) and no pathological complete response (pCR). Similar genomic alterations are observed before and after treatment without influencing the efficacy of subsequent EGFR-tyrosine kinase inhibitors (TKIs) in vitro . Infiltration and T cell receptor (TCR) clonal expansion of CCR8 + regulatory T (Treg) hi /CXCL13 + exhausted T (Tex) lo cells define a subtype of EGFR-mutant NSCLC highly resistant to immunotherapy, with the phenotype potentially serving as a promising signature to predict immunotherapy efficacy. Informed circulating tumor DNA (ctDNA) detection in EGFR-mutant NSCLC could help identify patients nonresponsive to neoadjuvant immunochemotherapy. These findings provide supportive data for the utilization of neoadjuvant immunochemotherapy and insight into immune resistance in EGFR-mutant NSCLC.
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CORROSION SCIENCE
·
2017
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IF 5.245
Mingjun Cui,Siming Ren,Songlv Qin,Qunji Xue,Haichao Zhao,Liping Wang
Hexagonal boron nitride (h-BN), a structural analogue of graphene, is a better alternative for anticorrosive coatings because of its electrical insulation properties. In this work, few-layer h-BN nanosheets are obtained by the exfoliation of stacked h-BN powders with poly(2-butyl aniline) (PBA) and incorporated into epoxy coatings for protecting metallic substrate against corrosion. Results show that as prepared composite coatings exhibit high impedance modulus and low water absorption, suggesting their superior performance of corrosion protection owing to the “labyrinth effect” of h-BN and passivation effect of PBA on the metal substrates.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2018
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IF 2.441
Guisheng Zhou,Yulong Zhang,Yang Li,Mengyue Wang,Xiaobo Li
Mogroside V, a natural compound isolated from the fruits of Siraitia grosvenorii (Swingle), is a promising candidate for anti-diabetic activity. The present study aims to develop a simple and practical strategy for comparing the in vivo metabolite profiling of mogroside V in healthy and type 2 diabetic (T2D) model rats. In this paper, a highly sensitive and rapid ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) with MetaboLynx™ software combined with mass defect filtering (MDF) method was established and successfully applied to detect and identify the metabolites in plasma, urine, bile and feces samples of healthy and model rats administrated with mogroside V. The distribution of metabolites in plasma, bile, urine and feces of healthy and model rats had obvious differences. A total of 23 metabolites were observed in healthy rats while 26 metabolites were detected in model rats. The results indicated that dehydrogenation , deoxidation , oxidation and isomerization were the major metabolic transformations of mogroside V. Additionally, it was noticed that the peak areas of metabolites in T2D rat plasma samples were much larger than those of metabolites in healthy rat plasma sample, whereas in T2D rat urine samples they were remarkably smaller compared with healthy rat urine sample. These high blood concentrations of metabolites might be beneficial for the treatment of T2D. The results of this study are valuable and important in understanding the metabolic process and therapeutic mechanism of mogroside V.
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ELECTROCHIMICA ACTA
·
2018
·
IF 5.116
Fangyan Liu,Xiang Chu,Haitao Zhang,Binbin Zhang,Hai Su,Long Jin,Zixing Wang,Haichao Huang,Weiqing Yang
Nickel hydroxide has shown extraordinary promise for hybrid supercapacitors because of the high theoretical capacity and low cost, but is usually limited by its low power density and complex synthetic process. Here, we report the design of a self-assembly three-dimensional (3D) porous Ni(OH) 2 for high-performance energy storage through a simple, cost-effective, and environmentally friendly solvothermal-precipitation method. The 3D structure with interconnected network provides superior electron transportation properties, and the hierarchical porous architecture facilitates quick ion diffusion. The Ni(OH) 2 obtained at 250 °C delivers a high capacitance of 2110 F g −1 at 1.0 A g −1 and a moderate cycling stability with 53% of the maximum specific capacitance over 2000 cycles at 5.0 A g −1 . Additionally, on integration of the prepared Ni(OH) 2 with hierarchically porous carbons as negative electrode material , the hybrid supercapacitor device delivers a high capacitance of 115 F g −1 and a high energy density of 40.9 W h kg −1 at a power density of 405 W kg −1 . This study not only provides a simply controllable growth method of the unique self-assembly 3D porous Ni(OH) 2 with high performance but also opens up a new approach to prepare various metallic oxides/hydroxides with excellent performance for highly promising practical energy-storage systems.
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APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Li Jiang,Junaid Ali Syed,Yangzhi Gao,Hongbin Lu,Xiangkang Meng
In the present paper, polyaniline (PANI) coating was electropolymerized in the presence of phosphoric acid with subsequent deposition of Ni(OH) 2 particles. The Ni(OH) 2 reinforced PANI coating significantly enhances the corrosion resistance of 304 stainless steel (304SS) in comparison with the pristine PANI coating. The galvanostatically deposited Ni(OH) 2 particles fill the pores of the pristine PANI coating and improves the coatings hydrophobicity which decreases the diffusion of aggressive media. Importantly, the R p values of Ni(OH) 2 reinforced PANI coating is much higher than that of pristine PANI coating and the Ni(OH) 2 reinforced PANI coating presents a long-term anti-corrosive ability (360 h) in 3.5 wt% NaCl solution. The prolonged corrosion protection of Ni(OH) 2 reinforced PANI coating is attributed to the improved physical barrier as well as the facile formation of passive oxide film that sustain the anodic protection of the coating.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Pan Song,Dong Lv,Luchen Yang,Jing Zhou,Xin Yan,Zhenghuan Liu,Kai Ma,Yunfei Yu,Xiaoyang Liu,Qiang Dong
Di-(2-ethylhexyl) phthalate (DEHP) might led to chronic and long-term effects on human organs due to its widespread use and bioaccumulation. Despite some cohorts reporting an association between DEHP exposure and BPH, its underlying mechanisms have not been investigated. Our findings indicate that exposure to DEHP or MEHP (main metabolites of DEHP in the human body) leads to increased prostate weights, elevated prostate index, and notable epithelial thickening in rats. It has been observed to promote BPH-1 cell proliferation with effects ranging from low to high concentrations. Transcriptome sequencing analysis of rat prostate tissues identified KIF11 as the key hub gene. KIF11 is highly expressed after DEHP/MEHP exposure, and knocking down of KIF11 inhibits the MEHP-induced promotion of cell proliferation. Exposure to MEHP has been observed to increase the expression of p-GSK-3β and elevate the levels of β-catenin, thereby activating the Wnt/β-catenin signaling pathway. Knocking down of KIF11 significantly inhibits these effects. Histone H3 at Lysine 27 acetylation (H3K27ac) is implicated in the upregulation of KIF11 expression, as evidenced by the addition of the acetylation inhibitor C646. In summary, our findings established that DEHP exposure could promote BPH through H3K27ac regulated KIF11/Wnt/β-catenin signaling pathway.
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FUEL
·
2024
·
IF 6.7
Qiuxiang Yao,Lei He,Duo Ma,Yong Zhang,Dan Zhang,Ming Sun
The naphthalene derivatives in coal tar naphthalene and anthracene oil fractions mostly contain short-chain alkyl groups. The enrichment of PAHs (polycyclic aromatic hydrocarbons, mainly including naphthalene, anthracene, and pyrene) by the dealkylation of PAHs-rich fraction in coal tar was assumed to obtain high-purity PAHs by distillation. Based on this strategy, using 2-methylnaphthalene as the model compound, and the influences of catalysts with different supports (SiO 2 , Al 2 O 3 , ZSM-5, Beta, and HY) and different active metal components (Ni, Co, Mo, and W) prepared by the equivalent-volume impregnation method on the hydro-dealkylation performance of 2-methylnaphthalene under different technological conditions (temperature, H 2 initial pressure and reaction time) were systematically investigated in a pressurized batch reactor. The experimental results show that the conversion rate of 2-methylnaphthalene is 81.23 % and the selectivity of naphthalene is 64.22 % under conditions of 480 °C, 2 MPa, 4 h, and 6 W-HY. Loading 0.8 wt% La on the 6 W-HY catalyst can improve the dispersion of active metal component W on the support, but it does not change the skeleton structure of the support. In addition, one-dimensional diffusion is the most probable reaction mechanism for 2-methylnaphthalene, and the evolution profiles of naphthalene can be reflected by the reaction mechanism with a reaction order of n = 4 using the model fitting method.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Changming Zhang,Zhihao Liu,Yongjin Zou,Fen Xu,Lixian Sun,Zhicong Hu,Sensen Yu,Cuili Xiang
Spinel-type materials are attracting great attention as potential electrocatalysts for the hydrogen- and oxygen-evolution reactions. However, their inherent catalytic activities are not on par with those of precious-metal catalysts, and their poor stabilities continue to constrain their rapid development. In this study, we fabricated Zr-CuCo 2 S 4 nanowires and Co(OH) 2 nanosheet–nanowires using electrochemical methods, resulting in lattice defects, sulfur vacancies, and more exposed active sites that catalyze the hydrogen- and oxygen-evolution reactions. DFT calculations revealed that sulfur vacancies enhance intrinsic activity, while Zr doping reduces the energy barrier for H*–OH* adsorption, promotes the formation of additional sulfur vacancies, and modulates active-site Cu/Co valence states, which greatly enhances water-dissociation and H*-adsorption kinetics. Consequently, Zr-CuCo 2 S 4 /Co(OH) 2 requires low overpotentials of 88 and 208 mV, respectively, to support highly stable alkaline hydrogen- and oxygen-evolution reactions at 10 mA cm −2 . This study provides a rational strategy for designing low-cost spinels for green and clean energy.
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