Immunity Inflammation and Disease
·
2024
·Vol. 12
·期 5
·e1229
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IF 3.1
Meng Zhang,Deyuan Zhi,Pei Liu,Yajun Wang,Meili Duan
Background Dioscin has many pharmacological effects; however, its role in sepsis-induced cardiomyopathy (SIC) is unknown. Accordingly, we concentrate on elucidating the mechanism of Dioscin in SIC rat model. Methods The SIC rat and H9c2 cell models were established by lipopolysaccharide (LPS) induction. The heart rate (HR), left ventricle ejection fraction (LVEF), mean arterial blood pressure (MAP), and heart weight index (HWI) of rats were evaluated. The myocardial tissue was observed by hematoxylin and eosin staining. 4-Hydroxy-2-nonenal (4-HNE) level in myocardial tissue was detected by immunohistochemistry. Superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) activities in serum samples of rats and H9c2 cells were determined by colorimetric assay. Bax, B-cell lymphoma-2 (Bcl-2), toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88), phosphorylated-p65 (p-p65), and p65 levels in myocardial tissues of rats and treated H9c2 cells were measured by quantitative real-time PCR and Western blot. Viability and reactive oxygen species (ROS) accumulation of treated H9c2 cells were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and dihydroethidium staining assays. Results Dioscin decreased HR and HWI, increased LVEF and MAP, alleviated the myocardial tissue damage, and reduced 4-HNE level in SIC rats. Dioscin reversed LPS-induced reduction on SOD, CAT, GSH, and Bcl-2 levels, and increment on Bax and TLR4 levels in rats and H9c2 cells. Overexpressed TLR4 attenuated the effects of Dioscin on promoting viability, as well as dwindling TLR4, ROS and MyD88 levels, and p-p65/p65 value in LPS-induced H9c2 cells. Conclusion Protective effects of Dioscin against LPS-induced SIC are achieved via regulation of TLR4/MyD88/p65 signal pathway.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Liudi Ji,Fei Li,Qi Jia,Yicheng Yao,Xiaoming Zhu,Zeyu Li,Peng Hu
Developing rapid and reliable electrochemical monitoring methods for 4-CP is of great significance to ensure the safety of the water environment. Electrochemical sensing materials with high sensitivity and selectivity are urgently needed. Here, interlaced sheet-like Ni-BDC frameworks (BDC: 1,4-dicarboxybenzene) and multi-walled carbon nanotubes (MWCNTs) composite (Ni-BDC/MWCNTs) are constructed via in-situ self-assembly of Ni-BDC in the presence of MWCNTs at room temperature . Because of the large specific surface area , high electrochemical activity of Ni-BDC, and the outstanding electrical conductivity of MWCNTs, the obtained Ni-BDC/MWCNTs compound had a significant signal enhancement effect for the oxidation of 4-CP based on the large electrochemical active area, fast electron transfer ability, strong accumulation ability, and high electrocatalytic rate. It was found that the electrochemical oxidation of 4-CP involved the transfer of an electron and a proton. A wide linear range (0.1–50 and 50–500 μM), low detection limit (16.5 nM), high reproducibility, strong stability, and good selectivity were achieved at the Ni-BDC/MWCNTs sensing platform. It also has been successfully used to monitor 4-CP in different river water samples, and got similar results compared to HPLC, which provides promising potential for application in water environment safety monitoring and protection.
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JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Tianyang Hou,Yuangang Xu,Ze Xu,Xiaopeng Zhang,Ming Lu
The formation of non-metallic pentazolate salts from the combination of simple nitrogen-rich cations with cyclo -N 5 − is expected to be applied in propellants and explosives. In this work, a series of structurally similar cations ([NH 3 CH 3 ] + , [NH 2 (CH 3 ) 2 ] + , [NH(CH 3 ) 3 ] + , and [N(CH 3 ) 4 ] + ) were used to synthesize four cyclo -N 5 − containing energetic salts, and the contribution of crystal stacking mode, hydrogen bonding, C–H··· π and π -stacking interactions in the stabilization of cyclo -N 5 − salts has been investigated by geometrical and Hirshfeld surface analyses as well as theoretical calculations. All salts have been thoroughly characterized by single-crystal X-ray diffraction, infrared (IR), multinuclear NMR ( 1 H and 13 C) spectroscopy, and elemental analysis. The decomposition temperatures of all salts are higher than 75 °C, as measured by thermogravimetric (TG). These compounds all show low sensitivities (IS ≥ 35 J, FS > 360 N) measured by standard BAM methods. Their heats of formation and detonation performances were calculated using the Born–Haber energy cycle and K-J method (“Pilem” program), respectively. This study has great significance for future design and synthesis of cyclo -N 5 − containing energetic materials.
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MATERIALS & DESIGN
·
2025
·
IF 7.9
Lifang Xie,Tianxiang Yuan,Su Hu,Chenmin Zhao,WenLi Huang,Wei Ding,Ye Zhang,Xianwen Hu
Chronic diabetic wounds exhibit impaired healing due to a complex interplay of pathological mechanisms, including persistent hyperglycemia, excessive matrix metalloproteinase-9 (MMP9) activity, and chronic inflammation, which disrupt tissue repair and regeneration. A central driver of this dysregulated healing is macrophage polarization imbalance, with excessive pro-inflammatory M1 and insufficient reparative M2 macrophages, prolonging inflammation and delaying tissue remodeling. To address these challenges, we developed a dual-responsive hydrogel composed of phenylboronic acid-functionalized polyethyleneimine (PEI-PBA), polyvinyl alcohol (PVA), and Benzaldehyde-PEG-Benzaldehyde (DF-PEG-DF) for controlled co-delivery of interleukin-4 (IL-4) and salvianolic acid A (SalA). This dynamically crosslinked hydrogel responds to the diabetic wound microenvironment, enabling pH- and glucose-triggered release. IL-4 promotes M2 macrophage repolarization, resolving inflammation and enhancing tissue repair, while SalA scavenges reactive oxygen species (ROS) and inhibits MMP9 activity, preserving extracellular matrix (ECM) integrity and supporting regeneration. In vivo, the hydrogel significantly accelerated wound closure in diabetic mice by promoting M2 polarization and reducing MMP9-mediated matrix degradation. This coordinated modulation of the inflammatory and proteolytic microenvironment attenuated inflammation, enhanced angiogenesis, and increased collagen deposition, collectively facilitating tissue regeneration. These findings suggest this intelligent hydrogel is a promising therapeutic strategy for chronic diabetic wound healing with translational potential.
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ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Quan Luo,Guan Zhou,Zhongxia Li,Jiangpeng Dong,Hang Zhao,Huifang Xu,Xuefeng Lu
The chiral amine ( R )-2-(1-aminoethyl)-4-fluorophenol has attracted increasing attentions in recent years in the field of pharmaceuticals because of its important use as a building block in the synthesis of novel anti-tumor drugs targeting tropomyosin receptor kinases. In the present study, a ω-transaminase (ωTA) library consisting of 21 ( R )-enantioselective enzymes was constructed and screened for the asymmetric biosynthesis of ( R )-2-(1-aminoethyl)-4-fluorophenol from its prochiral ketone. Using ( R )-α-methylbenzylamine, D-alanine, or isopropylamine as amino donor, 18 ωTAs were identified with target activity and the enzyme AbTA, which was originally identified from Arthrobacter sp. KNK168, was found to be a potent candidate. The E. coli whole cells expressing AbTA could be used as catalysts. The optimal temperature and pH for the activity were 35–40 °C and pH8.0, respectively. Simple alcohols (such as ethanol, isopropanol, and methanol) and dimethyl sulfoxide were shown to be good cosolvents. High activities were detected when using ethanol and dimethyl sulfoxide at the concentrations of 5–20%. In the scaled-up reaction of 1-liter containing 13 mM ketone substrate, about 50% conversion was achieved in 24 h. 6.4 mM ( R )-2-(1-aminoethyl)-4-fluorophenol was generated. After a simple and efficient process of product isolation and purification (with 98.8% recovery), 0.986 g yellowish powder of the product ( R )-2-(1-aminoethyl)-4-fluorophenol with high ( R )-enantiopurity (up to 100% enantiomeric excess) was obtained. This study established an overall process for the biosynthesis of the high value pharmaceutical chiral amine ( R )-2-(1-aminoethyl)-4-fluorophenol by ωTA. Its applicable potential was exemplified by gram-scale production.
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CHEMISTRY & BIODIVERSITY
·
2024
·
IF 2.3
Yongchang Bi,Jingjun Wang,Xingyu Bian,Mingyue Chen,Guangyuan Zhu,Yumin Zhang,Qiang Gu
An efficient and green method for synthesizing 3-methyl-4-(hetero) arylmethylene isoxazole-5(4 H )-ones was developed using a recyclable and environmental–friendly catalyst, Na 2 S 2 O 3 , and 16 target compounds were successfully synthesized under the obtained optimal reaction condition. Using rifampicin as a positive control, the antibacterial activity of all synthesized compounds was tested by micro dilution method, among them, 3-methyl-4-[(2,4-dimethoxyphenyl) methylene]-isoxazole-5-one ( 4 m ) presented wonderful antimicrobial activity, which may contribute to the development of anti-tuberculosis drugs. Graphical
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Toxins
·
2019
·Vol. 11
·期 8
·447
·
IF 3.895
Li Liu,Zafar Iqbal Tanveer,Keqiu Jiang,Qingwen Huang,Jinghui Zhang,Yongjiang Wu,Zheng Han
With the widespread contamination of ochratoxin A (OTA), it is of significant importance for detecting OTA in foods and traditional Chinese medicine (TCM). In this study, a novel label-free fluorescent aptasensor utilizing the interaction between OTA-triggered antiparallel G-quadruplex and (N-methyl-4-pyridy) porphyrin (TMPyP) for the rapid and sensitive determination of OTA was established. The fluorescence of CdTe quantum dots (QDs) could be quenched by TMPyP. In the presence of analyte (OTA), the aptamer could recognize OTA and transform from a random coil to the antiparallel G-quadruplex. The interaction between G-quadruplex and TMPyP could release CdTe QDs from TMPyP, and thus recover the fluorescence of CdTe QDs. Under optimized conditions, the detection limit of the designed aptasensor was 0.16 ng mL−1, with a linear range of 0.2 to 20 ng mL−1. Furthermore, this aptasensor showed high selectivity toward OTA against other structural analogs and other mycotoxins, and was successfully applied inAstragalus membranaceussamples. The presented aptasensor for OTA detection could be a promising tool for the field monitoring of food and TCM.Keywords:ochratoxin A;porphyrin;quantum dots;G-quadruplex aptamerKey Contribution:A label-free fluorescent aptasensor for the sensitive determination of ochratoxin A utilizing the interaction between OTA-triggered G-quadruplex and TMPyP is reported.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Shuyu Tang,Hongxi Zhang,Ji Li,Jun Huang,Liang Wei,Jing Yang,Xiande Yang
The valorization of lignin into valuable chemicals is a cornerstone of future biorefineries, but it is often hindered by the need for harsh reaction conditions. Electrochemical oxidation offers a promising green alternative, yet many systems lack mechanistic clarity, which impedes rational design. Herein, we report a simple and highly efficient electrochemical system using tetrabutylammonium tetrafluoroborate (TBABF4) in methanol for the selective cleavage of lignin’s β-O-4 ether bond under ambient conditions. On a model compound, this metal-free system achieved over 97% conversion with excellent selectivity toward high-value aldehyde products. Mechanistic studies revealed a dual-pathway mechanism. Crucially, when this mechanistically guided protocol was applied to bagasse lignin, it demonstrated effective depolymerization, leading to the significant cleavage of native β-O-4 bonds, resulting in a total liquid product yield of 15.6%, which included valuable aromatic products such as benzaldehyde dimethyl acetal (7.9% yield). This work not only presents a practical method for lignin upgrading but also provides a clear mechanistic roadmap for the future design of selective electrocatalytic processes.
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PROCESS BIOCHEMISTRY
·
2021
·
IF 3.757
Huabiao Miao,Rui Jiang,Nanyu Han,Yu Ma,Qian Wu,Yuelin Mu,Zunxi Huang
Amylases have been widely applied for starch saccharification in the food, paper, pharmaceutical, and textile industries. In the present study, α-amylase DL-3-4−1 from B. amyloliquefaciens was successfully expressed in B. subtilis. The enzymatic activity exhibited high stability in a pH range from 4.0 to 11.0 and moderate thermostability. Furthermore, excellent stability was observed when incubated with metal ions, detergents, inhibitors, and organic solvents. Resistance against ethanol, urea, and guanidine−HCl was also observed. More importantly, a strategy to enhance the extracellular expression of α-amylase DL-3-4−1 was developed using 173 Sec-type signal peptides (SPs) as a library based on a fast and sequence-independent method. Fourteen SPs were identified with significantly increased yields of DL-3-4−1 via a high-throughput assay. Specifically, the enzymatic activity of the α-amylase-producing strain containing the signal peptide SP5D10 (YomL) was 3.74-fold higher than that of the strain with the wild type signal peptide (87.30 U/OD). In brief, the maximum specific activity of an α-amylase-producing strain containing the YomL signal peptide was 326.45 U/OD. This is the first report of an α-amylase that exhibits ethanol, urea, and guanidine−HCl tolerance. Hence, this α-amylase represents a promising candidate for future use in industry.
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JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
·
2020
·Vol. 79
·期 12
·1354-1364
·
IF 2.923
Chen Xing,Liu Kewei,Lin Zhenzhou,Huang Kaibin,Pan Suyue
This study aimed to evaluate whether the knockout of transient receptor potential melastatin 4 (TRPM4) could reduce cerebral edema and improve neurologic outcome in a mouse model of status epilepticus (SE). Wild-type (WT) (n = 61) and Trpm4–/– mice (n = 61) with behavioral seizures induced by lithium (10 mEq/kg) and pilocarpine (30–40 mg/kg) were terminated 2.5 hours after the onset of SE. After SE, 28 WT-SE and 27 Trpm4–/–-SE mice were observed for 28 days and assessed for survival and cognitive function; the others were killed after 24 hours, 72 hours, or 7 days, and evaluated for cerebral edema and histological injury. In comparison to WT-SE mice, the mortality and cognitive deficit for Trpm4–/–-SE mice following SE after 28 days were significantly ameliorated. Trpm4–/–-SE mice also showed less water content and cerebral edema assessed by magnetic resonance imaging, and decreased blood-brain barrier breakdown after SE. Moreover, Trpm4 deficiency significantly mitigated neuronal loss, cellular necrosis and apoptosis in the hippocampus and piriform cortex and mitigated astrocytosis and microgliosis. In conclusion, this study suggests that Trmp4 may represent a new target for improving outcomes after SE.
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EXPERIMENTAL DERMATOLOGY
·
2020
·Vol. 30
·期 2
·237-248
·
IF 3.368
Liu Tang,Xiaoqin Cao,Xiaolei Li,Hong Ding
Atopic dermatitis (AD) is a multifactorial chronic inflammatory skin disease characterized by skin barrier dysfunction, eczematous lesions, pruritus, and abnormal immune responses. In this study, we assessed the therapeutic effect of topical applied conjugated linoleic acid (CLA) on a murine AD model that was developed by repetitive applications of 2, 4-dinitrofluorobenzene (DNFB). 2% or 5% CLA could markedly ameliorate AD-like skin lesions, scratching behaviour and skin inflammation as evidenced by the reduced inflammatory blood cells, IgE and Th2-related cytokine levels, and the infiltration of mast cells and inflammatory cells to the dermal tissues. Moreover, topical application with CLA modulated skin barrier repair including maintaining a balanced skin pH and increasing skin hydration, partially mediated by upregulating skin barrier-related protein, filaggrin (FLG). In addition, topical CLA significantly dose-dependently inhibited pro-inflammatory cytokines including interleukin (IL)-6, IL-1β, tumour necrosis factor (TNF)-α and pro-inflammatory enzyme expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in inflamed mice skin. Its anti-inflammatory effect was associated with the inhibition of DNFB-stimulated IκBα and NF-κB p65 phosphorylation in mouse skin. Taken together, our results suggest that locally applied CLA exerts potentially protective effects against AD lesional skin at least in part, due to regulation of skin barrier function and inflammatory response.
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PROGRESS IN PHOTOVOLTAICS
·
2015
·Vol. 23
·期 12
·1828-1836
·
IF 7.584
Xinsheng Liu,Chao Chen,Liang Wang,Jie Zhong,Miao Luo,Jie Chen,Ding-Jiang Xue,Dengbing Li,Ying Zhou,Jiang Tang
Sb 2 Se 3 has attracted great research interest very recently as a promising absorber material for thin film photovoltaics due to its suitable bandgap, high absorption coefficient, and non-toxic, low cost, and earth abundant nature. In this work, a significant efficiency improvement to 4.8% of superstrate cadmium sulfide (CdS)/Sb 2 Se 3 solar cells is obtained by the controlled addition of oxygen during thermal evaporation of Sb 2 Se 3 films. Systematic materials and device physics characterization reveal that oxygen addition during Sb 2 Se 3 film evaporation significantly improves the CdS/Sb 2 Se 3 heterojunction quality through effective passivation of interfacial defect states, resulting in a substantial enhancement in device circuit voltage and short circuit current density. The 4.8% device is the highest efficiency thus far reported for Sb 2 Se 3 thin film solar cells. Copyright © 2015 John Wiley & Sons, Ltd.
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