Toxins
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2019
·Vol. 11
·期 8
·447
·
IF 3.895
Li Liu,Zafar Iqbal Tanveer,Keqiu Jiang,Qingwen Huang,Jinghui Zhang,Yongjiang Wu,Zheng Han
With the widespread contamination of ochratoxin A (OTA), it is of significant importance for detecting OTA in foods and traditional Chinese medicine (TCM). In this study, a novel label-free fluorescent aptasensor utilizing the interaction between OTA-triggered antiparallel G-quadruplex and (N-methyl-4-pyridy) porphyrin (TMPyP) for the rapid and sensitive determination of OTA was established. The fluorescence of CdTe quantum dots (QDs) could be quenched by TMPyP. In the presence of analyte (OTA), the aptamer could recognize OTA and transform from a random coil to the antiparallel G-quadruplex. The interaction between G-quadruplex and TMPyP could release CdTe QDs from TMPyP, and thus recover the fluorescence of CdTe QDs. Under optimized conditions, the detection limit of the designed aptasensor was 0.16 ng mL−1, with a linear range of 0.2 to 20 ng mL−1. Furthermore, this aptasensor showed high selectivity toward OTA against other structural analogs and other mycotoxins, and was successfully applied inAstragalus membranaceussamples. The presented aptasensor for OTA detection could be a promising tool for the field monitoring of food and TCM.Keywords:ochratoxin A;porphyrin;quantum dots;G-quadruplex aptamerKey Contribution:A label-free fluorescent aptasensor for the sensitive determination of ochratoxin A utilizing the interaction between OTA-triggered G-quadruplex and TMPyP is reported.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2021
·
IF 4.384
Ziqing Wang,Wenwen Mu,Pengxiao Li,Guoyun Liu,Jie Yang
Curcumin was reported as an anti-inflammatory agent. However, curcumin's poor bioavailability limited its clinical utility. Here, thirty ortho -substituted mono-carbonyl curcumin derivatives, containing acetone, cyclopentanone, cyclohexanone or 4-piperidione ( N H, N -methyl or N -acrylyl) moieties replacing β-diketone moiety of curcumin, were investigated for anti-inflammatory activity. Two active ortho -trifluoromethoxy-substituted 4-piperidione-containing derivatives 22 and 24 owned good cell uptake ability, and displayed excellent anti-inflammatory activity in both lipopolysaccharide-induced Raw264.7 macrophages and a dextran sulfate sodium (DSS)-induced mouse model of colitis . They inhibited the production of nitric oxide , reactive oxygen species , malonic dialdehyde and cyclooxygenase-2; and the expression of pro-inflammatory cytokines interleukin-1β, tumor necrosis factor-α and myeloperoxidase ; the phosphorylation of mitogen-activated protein kinases; and the nucleus translocation of p65. What's more, 22 or 24 oral administered reduced the severity of clinical symptoms of ulcerative colitis (body weight and disease activity index), and reduced obviously DSS-induced colonic pathological damage (the colon length and histopathology analysis). These results suggested that ortho -trifluoromethoxy-substituted 4-piperidione-containing mono-carbonyl curcumin derivatives 22 and 24 were potential anti-inflammatory agents; and offered the important information for design and discovery of more potent anti-inflammatory drug candidates.
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PROCESS BIOCHEMISTRY
·
2021
·
IF 3.757
Huabiao Miao,Rui Jiang,Nanyu Han,Yu Ma,Qian Wu,Yuelin Mu,Zunxi Huang
Amylases have been widely applied for starch saccharification in the food, paper, pharmaceutical, and textile industries. In the present study, α-amylase DL-3-4−1 from B. amyloliquefaciens was successfully expressed in B. subtilis. The enzymatic activity exhibited high stability in a pH range from 4.0 to 11.0 and moderate thermostability. Furthermore, excellent stability was observed when incubated with metal ions, detergents, inhibitors, and organic solvents. Resistance against ethanol, urea, and guanidine−HCl was also observed. More importantly, a strategy to enhance the extracellular expression of α-amylase DL-3-4−1 was developed using 173 Sec-type signal peptides (SPs) as a library based on a fast and sequence-independent method. Fourteen SPs were identified with significantly increased yields of DL-3-4−1 via a high-throughput assay. Specifically, the enzymatic activity of the α-amylase-producing strain containing the signal peptide SP5D10 (YomL) was 3.74-fold higher than that of the strain with the wild type signal peptide (87.30 U/OD). In brief, the maximum specific activity of an α-amylase-producing strain containing the YomL signal peptide was 326.45 U/OD. This is the first report of an α-amylase that exhibits ethanol, urea, and guanidine−HCl tolerance. Hence, this α-amylase represents a promising candidate for future use in industry.
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Crystals
·
2019
·Vol. 9
·期 9
·474
·
IF 2.061
Xunan Shen,Chengchun Tang,Chao Zhang,Guanda Li,Yue Zhao,Wei Li,Guifeng Chen,Tie Yang
In current work, Cu2ZnSn(S,Se)4thin films have been prepared by the sol-gel method based on dimethyl sulfoxide solution followed by a modified three-step selenization process. The key process of this method is to divide the Se evaporation and annealing into two different stages: employ a thermal cracking Se source in the Se evaporation stage and an above-atmospheric pressure in the annealing process. The morphological, structural, elemental distributional, and photovoltaic properties of Cu2ZnSn(S,Se)4thin films prepared with the three-step selenization process were systematically investigated. It was found that through this modified selenization process, the formations of secondary phases (ZnSe, CuSnSe3) and a fine-grain bottom layer, which usually exists in the traditional one-step selenization process, were effectively suppressed. These improvements could further reduce the carrier recombination and improve the solar cell performance. The best solar cell is obtained with a short-circuit current density of 28.16 mA/cm2, open-circuit voltage of 404.91 mV, fill factor of 62.91%, and a power conversion efficiency of 7.17% under air mass 1.5 (100 mW/cm2) illumination.Keywords:CZTSSe solar cell;sol-gel method;selenization;thermal cracking Se;above-atmospheric pressure;secondary phases
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JOURNAL OF POWER SOURCES
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2021
·
IF 9.127
Zhaoyu Sun,Hebing Zhou,Xuehuan Luo,Yanxia Che,Weishan Li,Mengqing Xu
A novel electrolyte additive designed as a cathode interface formation enhancer for high voltage lithium-ion battery (LIB) systems is investigated. Lithium 4-benzonitrile trimethyl borate (LBTB) is synthesized by a two-step reaction, and the results show that the cycling performance of Li/LiCoO 2 cell at 4.4 V is significantly improved from 15.08% to 73.21% by adding 2.0 wt% of LBTB to the electrolyte (1.0 M LiPF 6 in EC/DEC/EMC = 3/2/5, wt.%). A combination of electrochemical methods , ex-situ spectroscopy characterization techniques and computational calculations are employed to investigate the mechanisms of action for this additive. Cyclic voltammograms indicate the additive is preferably oxidized on the LiCoO 2 surface prior to the electrolyte oxidation and results in a more stable and robust passivation layer than the electrolyte alone. Computational calculations support the observed sacrificial oxidation of LBTB prior to the electrolyte solvents decomposition. The CEI formed by LBTB decomposition mitigates the dissolution of transition metal and subsequently improves the cycling stability of the LiCoO 2 cathodes at high voltages. Surface characterization techniques reveal the presence of -B-O- products derived from LBTB decomposition on the surface of the LCO cathode.
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Hanxun Qiu,Feilong Qiu,Xuebin Han,Jing Li,Junhe Yang
Here we report an environmentally friendly, one-pot strategy toward preparation of nickel nanoparticle-decorated reduced graphene-oxide (Ni-RGO) nanostructures, by employing Ni(AC) 2 as nickel source and ethylene glycol as both solvent and reducing agent via a facile microwave irradiation heating approach. The results show that Ni nanoparticles with an average diameter of around 40 nm are homogeneously anchored onto the surface of RGO sheets. As compared to the pure Ni nanoparticles and RGO sheets, Ni-RGO composites with over 64 wt% loading of Ni nanoparticles possess superior catalytic activities and selectivity toward the reduction of 4-nitrophenol. The corresponding kinetic reaction rate constant (defined as κ ) is even four-fold compared to pure Ni nanoparticles. Such promising composites show great potential for friendly treatment of industrial waste containing nitrophenol in a simple, sustainable and green way.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Xiaogang Wang,Yu Bai,Qiang He,Jianye Li,Sun Wang,Wei Guo,Xiaozheng Sun
A novel fluorescent probe , fluorescent dialdehyde cellulose (FDAC), was prepared to detect p -phenylenediamine (PPD) in water samples conveniently and quickly. This was achieved by grafting 7-amino-4-methylcoumarin (AMC) onto dialdehyde cellulose (DAC) via an aldol–amine condensation reaction. This method is greener, more economical, and simpler than existing methods for preparing fluorescent probes. The probe was found to be more effective for PPD detection in polar solvents, with less interference from pH and other compounds present in the sample matrix. The photoluminescence of FDAC at λ ex /λ em = 340/430 nm was statically quenched by PPD, allowing for accurate detection within the range of 10–100 μmol/L under optimal conditions, with a detection limit of 3.2 μmol/L (3 σ/s). Meanwhile, the Schiff base (–C=N– group) generated by the condensation of DAC and AMC increased the reaction activity of the fluorescent moiety and changed the AMC conjugated structure, making FDAC more susceptible to aminolysis with PPD than AMC. This study presents a promising solution for fluorescence detection of aniline compounds, with significant potential for application in fields such as environmental analysis.
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JOURNAL OF ORGANIC CHEMISTRY
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2021
·
IF 4.354
Qingqiang Tian,Shasha Xu,Chiying Zhang,Xinyi Liu,Xiangwei Wu,Yahui Li
Herein, an interesting palladium-catalyzed procedure for the direct carbonylative thiomethylation of aromatic amine derivatives with 4-methylthio-2-butanone is developed. Using 4-methylthio-2-butanone as (methylthio) transfer agent, a variety of corresponding thioesters are obtained with moderate to good yields under base-free condition. In addition, good functional group tolerance can be observed.
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Journal of Materials Chemistry A
·
2019
·Vol. 7
·期 32
·18940-18949
·
IF 10.733
Xiaohao Liu,Linbin Tang,Zhi Li,Jianhua Zhang,Qunjie Xu,Haimei Liu,Yonggang Wang,Yongyao Xia,Yuliang Cao,Xinping Ai
The low energy density and the unsatisfactory cycling stability of cathode materials in sodium-ion battery systems have restricted their wider application. Here, a representative high voltage cathode of Na4Co3(PO4)2P2O7 (NCPP) was modified through Al doping. The as-prepared Na3.85Co2.85Al0.15(PO4)2P2O7 (Al0.15-NCPP) can deliver a discharge capacity of 99.5 mA h g−1 at 0.5C. A superior rate capability (80.3 mA h g−1 at 20C and 73.4 mA h g−1 at 50C) as well as excellent ultralong cycling stability (96.3% capacity retention after 900 cycles at 10C and 82.7% capacity retention after 8000 cycles at 30C) can be obtained. The excellent sodium storage properties are benefited from the advantages of Al doping. Al doping not only enhances ionic conductivity, but also improves the structural stability. Al0.15-NCPP, which has outstanding electrochemical properties, has been proposed as a very attractive high voltage cathode material for sodium-ion batteries. More impressively, a full cell assembled with an Al0.15-NCPP cathode and hard carbon (HC) anode exhibits distinguished rate/cycling performances (70.6 mA h g−1 at 30C and 95% capacity retention after 200 cycles at 1C) and an extremely high output voltage of 4.36 V, thus providing a preview of its practical application. In addition, our research can be used to enhance the electrochemical properties of other polyanion-based high voltage electrode materials.
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Forensic Toxicology
·
2016
·Vol. 34
·期 2
·398-402
·
IF 0
Shintani-Ishida Kaori,Kakiuchi Yasuhiro,Ikegaya Hiroshi
The toxicological detection of the new synthetic cathinone 4′-methyl-α-pyrrolidinohexanophenone (MPHP) in urine samples has been impossible, because much of MPHP is metabolized before its excretion into urine. In this study, we successfully quantified unmetabolized MPHP in urine of an autopsy case using a sensitive method by liquid chromatography–time-of-flight-mass spectrometry. The quantification method showed good linearity in the range of 1.00–100 ng/mL, and the limit of detection was 0.5 ng/mL in human urine. In the autopsy case, the concentrations of MPHP in urine, plasma, and liver tissue samples were determined to be 60.1, 32.9 ng/mL, and 63.1 ng/g, respectively.
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Frontiers in Plant Science
·
2022
·
IF 6.627
Lei Wenli,Song Yuyang,Long Ai,Que Yanyan,He Shuzhong,Zhong Hang,Chen Yang
The medicinal plants were wildly library of natural products in drug discovery. The most active molecules with seven-membered rings skeleton represent a challenge for construction. A stereoselectivity (4 + 3) cycloadditions between allenyl ethers and substituted furans induced by chiral auxiliaries has been investigated. And the results showed significant stereoselectivities and regioselectivities. The optical cycloadducts with an oxygen-substituted seven-membered ring framework were generated by removing chiral auxiliaries under acidic conditions. The antiproliferative activity of the novel compounds displayed moderate antiproliferative effects toward T47D cells.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Xiangxin Meng,Jianguo Deng,Qing Sun,Beibei Zong,Zizhao Zhang,Bo Shen,Bonan Kang,S. Ravi P. Silva,Lijun Wang
Perovskite solar cells (PSCs) have become a promising photovoltaic (PV) technology. Meanwhile, developing an electron transport layer (ETL) has been an effective way to promote the power conversion efficiency (PCE) of PSCs. Here, a 4-morpholine ethane sulfonic acid sodium salt (MES Na + ) doped SnO 2 ETL is utilized in planar heterojunction PSCs. The results show that the MES Na + doped ETL can improve the crystallinity , and absorbance of perovskite films, and passivate interface defects between the perovskite film and SnO 2 ETL. The doping effect accounts for the enhancement of conductivity and the decreasing work function of SnO 2 . When 10 mg mL −1 MES Na + was added to the SnO 2 precursor solution, the device showed the best performance Jsc, Voc, and FF of the PSCs values, which were 23.88 mA cm −2 , 1.12 V and 78.69%, respectively, and the PCE was increased from 17.43% to 21.05%. This doping ETL strategy provides an avenue for defect passivation to further increase the efficiency of perovskite solar cells.
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