Frontiers in Microbiology
·
2023
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IF 5.2
Miao Lei,Chen Siyuan,Yang Hua,Hong Yaqi,Sun Liwen,Yang Jie,Sun Guanjun,Liu Yi,Li Chunyan,Zang Hailian,Cheng Yi
Triclocarban (TCC), an emerging organic contaminant (EOC), has become a severe threat to soil microbial communities and ecological security. Here, the TCC-degrading strain Rhodococcus rhodochrous BX2 and DCA-degrading strain Pseudomonas sp. LY-1 (together referred to as TC1) were immobilized on biochar to remove TCC and its intermediates in TCC-contaminated soil. High-throughput sequencing was used to investigate the microbial community structure in TCC-contaminated soil. Analysis of co-occurrence networks was used to explore the mutual relationships among soil microbiome members. The results showed that the immobilized TC1 significantly increased the removal efficiency of TCC from 84.7 to 92.7% compared to CK (no TC1 cells on biochar) in 10 mg/L TCC liquid medium. The utilization of immobilized TC1 also significantly accelerated the removal of TCC from contaminated soil. Microbial community analysis revealed the crucial microorganisms and their functional enzymes participating in TCC degradation in soil. Moreover, the internal labor division patterns and connections of TCC-degrading microbes, with a focus on strains BX2 and LY-1, were unraveled by co-occurrence networks analysis. This work provides a promising strategy to facilitate the bioremediation of TCC in soil, which has potential application value for sustainable biobased economies.
研究领域:
环境科学
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微生物学
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
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IF 4.192
Long Zhang,Ping Hang,Xi-Yi Zhou,Wen-Jing Qiao,Jian-Dong Jiang
Dichlorprop [(RS)-2-(2,4-dichlorophenoxy)propanoic acid; DCPP], an important phenoxyalkanoic acid herbicide (PAAH), is extensively used in the form of racemic mixtures (Rac-DCPP), and the environmental fates of both DCPP enantiomers [(R)-DCPP and (S)-DCPP] mediated by microorganisms are of great concern. In this study, a bacterial strain Sphingopyxis sp. DBS4 was isolated from contaminated soil and was capable of utilizing both (R)-DCPP and (S)-DCPP as the sole carbon source for growth. Strain DBS4 preferentially catabolized (S)-DCPP as compared to (R)-DCPP. The optimal conditions for Rac-DCPP degradation by strain DBS4 were 30 °C and pH 7.0. In addition to Rac-DCPP, other PAAHs such as (RS)-2-(4-chloro-2-methylphenoxy)propanoic acid, 2,4-dichlorophenoxyacetic acid, 4-chloro-2-methylphenoxyacetic acid, and 2,4-dichlorophenoxyacetic acid butyl ester could also be catabolized by strain DBS4. Bioremediation of Rac-DCPP-contaminated soil by inoculation of strain DBS4 exhibited an effective removal of both (R)-DCPP and (S)-DCPP from the soil. Due to its broad substrate spectrum, strain DBS4 showed great potential in the bioremediation of PAAH-contaminated sites.
研究领域:
环境微生物学
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生物修复
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化学生态学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
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2024
·Vol. 46
·期 9
·1-17
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IF 3.2
Zhong Bin,Xie Hanyi,Pan Tao,Su Buli,Xu Weijun,Wu Zhenqiang
Highly acidic citrus pomace (CP) is a byproduct of Pericarpium Citri Reticulatae production and causes significant environmental damage. In this study, a newly isolated acid-tolerant strain of Serratia sp. JS-043 was used to treat CP and evaluate the effect of reduced acid citrus pomace (RACP) in passivating heavy metals. The results showed that biological treatment could remove 97.56% of citric acid in CP, the organic matter in the soil increased by 202.60% and the catalase activity in the soil increased from 0 to 0.117 U g −1 . Adding RACP into soil can increase the stabilization of Cu, Zn, As, Co, and Pb. Specifically, through the metabolism of strain JS-043, RACP was also involved in the stabilization of Zn and Pb, and Residual Fraction in the total pool of these metals increased by 10.73% and 10.54%, respectively. Finally, the genome sequence of Serratia sp. JS-043 was completed, and the genetic basis of its acid-resistant and acid-reducing characteristics was preliminarily revealed. JS-043 also contains many genes encoding proteins associated with heavy metal ion tolerance and transport. These findings suggest that JS-043 may be a high-potential strain to improve the quality of acidic organic wastes that can then be useful for soil bioremediation. Graphical Abstract
研究领域:
环境科学
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微生物学
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生物技术
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土壤学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
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IF 5.589
Shanshan Sun,Haizhen Wang,Binxin Fu,Hao Zhang,Jun Lou,Laosheng Wu,Jianming Xu
Rhodococcus sp. WB9, a strain isolated from polycyclic aromatic hydrocarbons contaminated soil, degraded phenanthrene (PHE, 100 mg L −1 ) completely within 4 days. 18 metabolites were identified during PHE degradation, including 5 different hydroxyphenanthrene compounds resulted from multiple routes of initial monooxygenase attack. Initial dioxygenation dominantly occurred on 3,4-C positions, followed by meta -cleavage to form 1-hydroxy-2-naphthoic acid (1H2N). More than 95.2% of 1H2N was transported to and kept in extracellular solution without further degradation. However, intracellular 1H2N was converted to 1,2-naphthalenediol that was branched to produce salicylate and phthalate. Furthermore, 131 genes in strain WB9 genome were related to aromatic hydrocarbons catabolism, including the gene coding for salicylate 1-monooxygenase that catalyzed the oxidation of 1H2N to 1,2-naphthalenediol, and complete gene sets for the transformation of salicylate and phthalate toward tricarboxylic acid (TCA) cycle. Metabolic and genomic analyses reveal that strain WB9 has the ability to metabolize intracellular 1H2N to TCA cycle intermediates, but the extracellular 1H2N can’t enter the cells, restricting 1H2N bioavailability and PHE mineralization.
研究领域:
环境微生物学
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生物降解
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代谢组学
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基因组学
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产品关联
DOI
PubMed
高影响因子
BIOLOGICAL CONTROL
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2014
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IF 1.873
Waseem Raza,Jun Yuan,Ning Ling,Qiwei Huang,Qirong Shen
The volatile organic compounds (VOCs) produced by antagonistic microbes have great antifungal potential against soil-borne fungal pathogens. The VOCs produced by Paenibacillus polymyxa strain WR-2 in the presence of root exudates and organic fertilizer were identified and their effects on the growth and spore germination of Fusarium oxysporum f. sp. niveum were evaluated. The VOCs produced by WR-2 inhibited the growth of F. oxysporum by 38%, 36% and 40% in agar medium, sterilized soil and natural soil, respectively. This inhibitory effect was increased to 60%, 58% and 64% with the addition of organic fertilizer in agar medium, sterilized soil and natural soil, respectively. The addition of root exudates did not affect the production of antifungal VOCs by WR-2. The VOCs produced by WR-2 completely inhibited the germination of F. oxysporum spores. Out of 42 identified VOCs, seven VOCs; benzothiazole, benzaldehyde, undecanal, dodecanal, hexadecanal, 2-tridecanone and phenol were found to inhibit the growth of F. oxysporum . The results of these experiments suggest another significance of using organic fertilizer as a carrier material with the biocontrol agents to control soil-borne fungal pathogens.
研究领域:
微生物学
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植物病理学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
·
IF 13.6
Jiawei Liu,Qingyi Zeng,Huirui Lei,Kaiyuan Xin,Anming Xu,Ren Wei,Ding Li,Jie Zhou,Weiliang Dong,Min Jiang
Microorganisms capable of decomposing polyurethane (PU) and other plastics have the potential to be used in bio-recycling processes. In this study, 20 PU-degrading strains were isolated, including 11 bacteria and 9 fungi, using a synthesized poly(1,4-butylene adipate)-based PU (PBA-PU) as the screening substrate. Three PU substrates with increasing structure complexities were used for a thorough evaluation of microbial degradation capacity: Impranil® DLN-SD, PBA-PU film and PU foam waste. After 4 days, the best fungal PBA-PU degrader, Cladosporium sp. P7, could degrade 94.5% of Impranil® DLN-SD. After 28 days of cultivation, 32.42% and 43.91% of solid PBA-PU film was converted into soluble small molecules when used as the sole carbon source or in a medium with other co-carbon sources, respectively. Accordingly, the weight loss of PU foam waste after 15 days was 15.3% for the sole carbon condition and 83.83% for the co-carbon conditions. Furthermore, PBA-PU was used for metabolic pathway analysis because of its known composition and chemical structure. Six metabolites were identified during the degradation process of PBA-PU, including adipic acid (AA), 1,4-butanediol (BDO), and 4,4’-methylenedianiline (MDA), which can also be used as the sole carbon source to grow the fungal strain P7, resulting in the discovery of two MDA metabolites during the cultivation processes. Based on the presence of these eight metabolites, we hypothesized that PBA-PU is first depolymerized by the fungal strain P7 via ester and urethane bond hydrolysis , followed by intracellular metabolism and mineralization of the three monomers to CO 2 and H 2 O.
研究领域:
环境科学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2016
·
IF 3.976
Hai-Ming Zhao,Huan Du,Jing Lin,Xue-Bin Chen,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Hua-Ming Qin,Ming-Hung Wong
A newly isolated strain Agromyces sp. MT-O could utilize various phthalates and efficiently degraded di-(2-ethylhexyl) phthalate (DEHP). Response surface methodology was successfully employed for the optimization of culture conditions including pH (7.2), temperature (29.6), and inoculum size (OD 600 of 0.2), resulting in almost complete degradation of DEHP (200 mg L − 1 ) within 7 days. At different initial concentrations (50–1000 mg L − 1 ), DEHP degradation curves were fitted well with the first-order kinetic model, and the half-life of DEHP degradation ranged from 0.83 to 2.92 days. Meanwhile, the substrate inhibition model was used to describe the special degradation rate with q max , K s , and K i of 0.6298 day − 1 , 86.78 mg L − 1 , and 714.3 mg L − 1 , respectively. The GC–MS analysis indicated that DEHP was degraded into mono-ethylhexyl phthalate and phthalate acid before its complete mineralization. Bioaugmentation of DEHP-contaminated soils with strain MT-O has greatly enhanced DEHP disappearance rate in soils, providing great potential for efficiently remediating DEHP-contaminated environment.
研究领域:
环境科学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
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IF 8
Junde Ren,Feifei Hu,Farooq Muhammad,Lihui Hu,Tao Song,Maninder Meenu,Jie Lin,Ying Liu,Hosahalli S. Ramaswamy,Yong Yu
This study investigated the freezing characteristics of Arctic yeast Leucosporidium sp. AY30 ice-binding protein (LeIBP) after heterologous expression by Aspergillus niger at different concentrations (0.01 %, 0.05 %, 0.1 %, 0.2 %, 0.5 %) and elucidated its effects on physicochemical quality, myofibrillar protein (MP) and ice crystal morphology of largemouth bass during air freezing. LeIBP achieved ice crystal regulation by significantly reducing the freezing point, super-cooling degree and phase transition time of the aqueous solution and prolonging the time of the maximum ice crystal formation zone (−1 to −5 °C). Its thermal hysteresis activity (THA) reached a peak at 0.2 % concentration (1.36 °C), which was superior to the THA of most traditional AFPs. Notably, the addition of 0.05 %LeIBP dramatically reduced the cross-sectional area and mean diameter of ice crystals by up to 91 % and 70 %, respectively. In addition, the addition of LeIBP better maintained the texture of fish, led to an increase in the L* value and a decrease in the b* value, and inhibited the decrease in the a* value, but decreased its water holding capacity slightly. Meanwhile, the addition of 0.01 %LeIBP better inhibited the reduction of MP content and maintained the stability of MP tertiary structure. The addition of LeIBP increased the α-helix content by 1.0–6.9 % to maintain the MP secondary structure, also significantly increased the ion bond content, hydrogen bond content, and hydrophobic interactions. In summary, the addition of low concentration (0.01 % and 0.05 %) of LeIBP better maintained the quality of fish meat during air freezing.
研究领域:
食品科学
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生物化学
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低温生物学
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微生物学
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蛋白质工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2019
·
IF 3.824
Shijuan Wu,Tengfei Li,Xian Xia,Zijie Zhou,Shixue Zheng,Gejiao Wang
Te(IV) reduction to Te(0) is a detoxification process in bacteria. Elemental Te nanostructures are new materials, however, the functions of them, such as adsorption ability, have not been fully explored. Shinella sp. WSJ-2 is a highly Te(IV)-resistant bacterium with a MIC of 8 mmol/L. It was able to rapidly reduce 0.3 mmol/L Te(IV) to Te nanorods (TeNRs) within 28 h, and its Te(IV) reduction ability was relatively stable after 14 cycles repeatedly. The size of TeNRs ranged from 50 to 120 nm. The surface of TeNRs was mainly enveloped with proteins and lipids. Moreover, the biogenic TeNRs were capable of removing multiple dyes and metals and were effectively recycled with 100 mmol/L NaCl. Adsorption equilibrium isotherms showed that the maximum adsorption amounts were 618.8, 1076.8, 496.3, 179.3, 58.7 and 275.3 mg/g for Methylene Blue , Azure B, Malachite Green, Cd(II), Cu(II) and Pb(II), respectively. In conclusion, this is the first evidence that the genus Shinella is involved in Te(IV) reduction. The strong sorption of cationic dyes and metals onto TeNRs is probably due to the electrostatic interaction. This study showed the potential of Shinella sp. WSJ-2 in the bioremediation of toxic Te(IV) and adsorption removal of multiple dyes and metals by biogenic TeNRs.
研究领域:
环境微生物学
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纳米技术
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生物修复
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产品关联
DOI
高影响因子
PLoS One
·
2018
·Vol. 13
·期 10
·e0204324
·
IF 2.766
Fangfang Li,Yidan Liu,Diwei Wang,Chaosheng Zhang,Zhihui Yang,Siqi Lu,Yangyang Wang
A halotolerant bacterial consortium capable of degrading di-(2-ethylhexyl) phthalate (DEHP) was enriched from activated sludge. Community analysis revealed that LF contained seven families and seven genera of bacteria. The predominant species was Gordonia sp. (54.93%), Rhodococcus . sp. (9.92%) and Achromobacter sp. (8.47%). The consortium could degrade 93.84% of 1000 mg/l DEHP after 48 h incubation. The optimal temperature and pH for LF to degrade DEHP were 30 °C and 6.0, respectively. LF degraded more than 91% of DEHP with salt concentrations ranging from 0–3%. The inoculum size had great effects on DEHP degradation (incubation time < 24h). LF could degrade high concentrations of DEHP (from 100 to 2000 mg/l) with the degradation ratio above 92% after 72 h incubation. Kinetics analysis revealed that the degradation of DEHP by LF was best fitted by the first-order kinetics when the initial concentration ranged from 100 to 2000 mg/l. The main intermediates (2-ethylhexyl pentyl phthalate, butyl (2-ethylhexyl) phthalate (BEHP), mono-ethylhexyl phthalate (MEHP), mono-hexyl phthalate (MHP), mono-butyl phthalate (MBP)) in DEHP degradation process were identified using gas chromatography–mass spectrometry (GC-MS), and a new complex biochemical pathway was proposed. Furthermore, LF could also degrade dimethyl phthalate (DMP), diethyl phthalate (DEP), di- n -butyl phthalate (DBP), di- n -octyl phthalate (DOP) and phthalic acid (PA).
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 17
·2729
·
IF 4.7
Xiaoqing Xu,Lingling Du,Meng Wang,Ran Zhang,Junjie Shan,Yu Qiao,Qing Peng,Bo Shi
Agrobacteriumsp. are notable for their ability to produce substantial amounts of exopolysaccharides. Our study identified an exopolysaccharide (Galacan, 4982.327 kDa) fromAgrobacteriumsp. FN01. Galacan is a heteropolysaccharide primarily composed of glucose and galactose at a molar ratio of 25:1. The FT-IR results suggested that Galacan had typical absorption peaks of polysaccharide. The results of periodate oxidation, Smith degradation, and NMR confirmed the presence of structural units, such as β-D-Galp(→, →3)β-D-Galp(1→, →2,3)β-D-Glcp(1→, β-D-Glcp(1→, and →2)β-D-Glcp(1→. Galacan demonstrated significant biological activities. In experiments conducted with zebrafish, it facilitated the proliferation ofLactobacillus brevisin the intestinal tract, suggesting potential prebiotic properties. Moreover, in vivo studies revealed its antihyperglycemic effects, as evidenced by significant reductions in blood glucose levels and enhanced fluorescence intensity of pancreatic β cells in a streptozotocin (STZ)-induced hyperglycemic zebrafish model. Additionally, antiaging assays demonstrated Galacan’s ability to inhibit β-galactosidase activity and enhance telomerase activity in a hydrogen peroxide (HP)-induced aging zebrafish model. These findings emphasized the potential of Galacan as a natural prebiotic with promising applications in diabetes prevention and antiaging interventions.
研究领域:
微生物学
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生物化学
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药理学
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分子生物学
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水生生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2023
·
IF 7.2
Minglei Lu,Shuting Liu,Haiping Zhu,Haifeng Huang,Chunying Lin,Jianxian Li,Baofang Zhang,Zefeng Zheng,Changsong Hu,Xiaoping Wu,Geli Liu,Tiejun Wang
Highly efficient releasing of hydrogen by aqueous-phase reforming of methanol (APRM) is of great significance to promote the development of stationary and mobile H 2 power supply for polymer electrolyte membrane fuel cells (PEMFCs). However, highly active catalysts with excellent performance of in situ releasing H 2 from APRM still face challenges. Herein, we report a Cu-SP/Al 2 O 3 –ZnO (Cu-SP/AZ) catalyst prepared by carbon layer encapsulated hierarchical porous microsphere strategy, which enhanced Cu dispersion (52.8 wt% loading, ∼5 nm particle size) on Al 2 O 3 –ZnO (AZ) microsphere . The proposed catalyst exhibited high H 2 production rate (base-free) of 64.23 μmolH 2 /gcat/s at 210 °C, which was nearly the same as that of 20% Pt/C catalyst (63.86 μmolH 2 /gcat/s) and 2.6-fold higher than that of commercial CuO/ZnO/Al 2 O 3 catalyst (24.62 μmolH 2 /gcat/s). The characterization results demonstrated that its high activity in APRM could be ascribed to the strategy of carbon layer encapsulated hierarchical porous microsphere as well as coordination of hydroxyl functional group of SP with Cu 2+ .
研究领域:
催化
·
化学工程
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材料科学
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能源技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）