Analytical Methods
·
2025
·
IF 2.6
Yaoting Mou,Lu Han,Yanhao Yin,Jianghua Liu,Yanqing Xu,Yuhang Tian,Yanfang Wu,Dongfei Chen,Yemin Guo,Xia Sun,Falan Li
Kanamycin (KAN) is an aminoglycoside antibiotic employed for the treatment of bacterial infections in livestock and poultry and is widely used as a veterinary drug in animal husbandry. Its overuse poses serious threats to public health and ecosystems; while traditional detection methods cannot meet the requirements of widespread application and rapid detection, developing rapid and reliable technology for KAN detection is essential. In this study, a colorimetric aptasensor was developed to rapidly detect KAN residues, leveraging the enhanced catalytic effect of aptamers on the peroxidase-like activity of the Cu2O/hemin-graphene oxide (Cu2O/hemin-GO) nanozyme. In the presence of hydrogen peroxide, the Cu2O/hemin-GO nanozyme demonstrated excellent peroxidase-like catalytic activity, oxidizing 3,3′,5,5′-tetramethylbenzidine (TMB). The adsorption of aptamers on the nanozyme surface increased the negative charge density and significantly enhanced the affinity of the nanozyme for the positively charged chromogenic substrate TMB, boosting the catalytic activity nearly threefold. Upon exposure to KAN, the specific binding of aptamers to KAN reduced their adsorption on the Cu2O/hemin-GO nanozyme, leading to a decrease in the peroxidase-like activity of the Cu2O/hemin-GO nanozyme and a corresponding reduction in color change. The aptasensor exhibited excellent sensitivity with a linear detection range from 0.1 pM to 1 μM and a detection limit of 16 fM. The recovery rates for KAN in milk samples ranged from 92.05% to 110.71%. Furthermore, the colorimetric aptasensor demonstrated high selectivity and reproducibility. Overall, the colorimetric approach achieved the economical, simple and sensitive detection of KAN residues, indicating its promising potential in real-world applications. In addition, this sensing method could be applied to other targets by replacing the aptamer, providing a strategy for developing sensors facilitating rapid detection.
研究领域:
生物化学
·
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Qian Bi,Feng Qiu,Ruo Yuan,Yun Xiang
The abnormal variation of the concentration of acid phosphatase (ACP) in human body can be related to several pathophysiological diseases. Herein, a highly sensitive and non-label electrochemical biosensor for detecting ACP on the basis of target-induced inhibition of metal ion/pyrophosphate (PPi) coordination and G-quadruplex/hemin nanowires signal amplification is described. The target ACP molecules can hydrolyze PPi into phosphate to prevent the coordination between Cu 2+ and PPi, and the un-coordinated Cu 2+ further triggers cyclic cleavage of a G-quadruplex sequence (GS)-containing hairpin probes by the Cu 2+ -dependent DNAzyems on the sensor electrode to release the GS, which leads to in situ formation of the GS/hemin nanowires via the stacking of the free GS on the surface-immobilized GS with the presence of hemin . The many hemin molecules in the nanowires can thus yield dramatically enhanced current response for detecting ACP sensitively with the detection limit of 0.45 U/L. Besides, low levels of ACP present in diluted serum samples can also be selectively detected with this method. In addition to its sensitivity and selectivity, the developed method can realize substantial signal amplifications without using enzymes or nanomaterials, which can facilitate the establishment of convenient and sensitive strategies for detecting low concentrations of phosphatase for disease diagnosis.
研究领域:
生物化学
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医学诊断
·
纳米技术
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Wendong Liu,Lin Chu,Chenghui Zhang,Pengjuan Ni,Yuanyuan Jiang,Bo Wang,Yizhong Lu,Chuanxia Chen
Revealing the relationship between composition and enzyme-like activities is essential for the rational designs of nanozymes and related assays with improved performances. Here, a series of Fe-N co-doped porous carbon (Fe-N-C) nanozymes containing different Fe contents have been successfully prepared by facilely pyrolyzing hemin trapped zeolitic-imidazolate-frameworks (Hemin@ZIF-8). The special doping agent hemin will endow Fe-N-C with exceptionally high peroxidase-mimetic activities due to the hierarchically porous structure, high specific surface area, and high dispersed Fe-N x sites. Notably, the optimal Fe 55 -N-C nanozymes are used to detect bio-enzymes, by taking alkaline phosphatase (ALP) as a model, based on the inhibition of nanozymatic activity by ascorbic acid (AA). A linear activity range of 0.05–20 U/L is obtained with a low detection limit of 0.03 U/L, which is superior to the most published optical ALP assays. This work provides not only a new method for the design of the transition metal-N-C nanozyme with enhanced peroxidase-like activity but also a promising strategy for the detection of ascorbic acid-generating bio-enzymes with the assistance of specific substrates.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 19
·8608-8618
·
IF 3.591
Min Chen,Haimei Li,Xueming Su,Runqiang Wu,Huafu Feng,Xiaohang Shi,Jintao Liang,Jiejing Chen,Guiyin Li
Glypican-3 (GPC3) is a potential hepatocellular carcinoma (HCC) serum marker with good sensitivity and specificity. Herein, a label-free electrochemical aptasensor for the detection of GPC3 was developed using a reduced graphene oxide–hemin–chitosan (RGO–H–CS) nanocomposite-modified screen-printed electrode (SPE) as a biosensing platform and GPC3 aptamer as the recognition element. When GPC3 was present, the aptamer could specifically bind with the target GPC3 by specific recognition reaction and form GPC3–aptamer conjugations on the sensing surface, which could alter the electrochemical redox signal of hemin (Fe(III)/hemin(Fe(II))) in the RGO–H–CS nanocomposite recorded by differential pulse voltammetry (DPV). The electrochemical GPC3 aptasensor was characterized via scanning electron microscopy (SEM), Raman spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, currents originating from the oxidation of hemin had a linear relationship with the concentration of GPC3 in the range of 0.01 µg mL−1 to 10.0 µg mL−1 with a detection limit of 7.9 ng mL−1 (S/N = 3). The developed aptasensor was applied to detect the GPC3 level in the human serum samples with the recovery rate of 101.04–104.9%. Moreover, the label-free aptasensor exhibited considerable anti-interfering ability, acceptable stability and good reproducibility. Therefore, this study provides a reliable and objective analysis method to achieve high screening for the early diagnosis of HCC.
研究领域:
生物传感器
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癌症诊断
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电化学
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纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2011
·
IF 1.77
Wenwen Liu,Wei Cao,Weihua Liu,Kang Du,Pixue Gong
This study established a novel flow injection (FI) methodology for the determination of phenol in aqueous samples based on luminol chemiluminescence (CL) detection. The method was based on the inhibition that phenol caused on the hemin-catalysed chemiluminescence reaction between luminol and hydrogen peroxide in alkaline solution. Optimum conditions and possible mechanisms have been investigated. The linear range was 2.0 × 10 −9 to 4.0 × 10 −7 g mL −1 for phenol. The proposed method is sensitive with a detection limit of 4.0 × 10 −10 g mL −1 . The relative standard deviation for 11 measurements was 2.3% for 1.0 × 10 −7 g mL −1 phenol. The method was applied for the determination of phenol in waste water samples. The results obtained compared well with those by an official method.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Neuroscience
·
2019
·
IF 3.648
Chen Xiao,Xi Zhiyu,Liang Huaibin,Sun Yuhao,Zhong Zhihong,Wang Baofeng,Bian Liuguan,Sun Qingfang
Secondary injuries mediated by oxidative stress lead to deterioration of neurological functions after intracerebral hemorrhage (ICH). Cortical astrocytes are among the most important cells in the central nervous system (CNS), and play key roles in maintaining redox homeostasis by providing oxidative stress defense. Hemin is a product of hemoglobin degradation, which has strong toxicity and can induce reactive oxygen species (ROS). Melatonin (Mel) and its metabolites are well tolerated without toxicity, prevent tissue damage as well as effectively assist in scavenging free radicals. We evaluated the hemin neurotoxicity to astrocytes and the resistance of Mel-treated astrocytes to hemin neurotoxicity. And we found Mel induced PKCα phosphorylation (p-PKC), nuclear translocation of Nrf2 in astrocytes, and upregulation of HO-1, which contributed to the reduction of ROS accumulation and cell apoptosis. Nrf2 and HO1 protein expression upregulated by Mel were decreased after administration of PKC inhibitor, Ro 31-8220 (Ro 31). Luzindole (Luz), a melatonin receptor inhibitor, suppressed p-PKCα, HO-1, and Nrf2 expression upregulated by Mel and increased cell apoptosis rate. The upregulation of HO-1 induced by Mel was depressed by knocking down Nrf2 expression by siRNA, which also decreased the resistance of astrocytes to toxicity of hemin. Mel activates astrocytes through PKCα/Nrf2/HO-1 signaling pathway to acquire resistance to toxicity of hemin and resist from oxidative stress and apoptosis. The positive effect of Mel on PKCα/Nrf2/HO-1 signaling pathway may become a new target for neuroprotection after intracerebral hemorrhage.
研究领域:
神经科学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2015
·
IF 7.435
Bin Jiang,Yuyuan Yao,Renjie Xie,Dejun Dai,Wangyang Lu,Wenxing Chen,Li Zhang
Porphyrin has received increasing interest in the catalysis field due to their excellent catalytic performance, nevertheless, the development of highly efficient porphyrin catalysts is still a significant challenge. In this work, a representative of persistent free radicals (PFRs), multiwalled carbon nanotubes (MWCNTs), was innovatively employed to construct an outstanding catalytic system, hemin-MWCNTs/H 2 O 2 . The introduction of MWCNTs greatly enhanced the catalytic activity of hemin, representing about 61 times higher reaction rate constant with the dye of methylene blue (MB) as probe compound, which was attributed to more reactive oxygen species (ROS) ( OH and hemin (Fe IV O)) generation. When MWCNTs were introduced to hemin/H 2 O 2 system, the decrease of PFRs concentration in MWCNTs were observed with larger number of trapped electrons, indicating that PFRs might transfer electrons to hemin, thus speeding up the reaction rate-determining step of hemin (Fe III ) to hemin (Fe II ), resulting in enhanced production of OH and hemin (Fe IV O). This study initiates an up-to-date research domain of PFRs-enhanced catalysis, paving the avenue toward developing robust processes for the efficient generation of ROS for applications in catalysis field.
研究领域:
化学催化
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环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xu Hun,Yan Meng,Shiyu Wang,Zhenghua Mei,Xiliang Luo
A nonlinear hybridization chain reaction (non L-HCR) is presented here, in which series of toehold-mediated strands hybridization and branch migration reaction initiate in the presence of a trigger DNA. It leads to self-sustained assembly of double-stranded substrates into fractal nanostructures. The formed fractal nanostructures were about 10 μm length × 1.5 μm wide × 30 nm high. Furthermore, the 3′ part (TAGGGTTGGGCGGGATGGGT) of Gx DNA are exposed and ready to combined with hemin to form hemin/G-quadruplex DNAzyme, which could amplify the generation of chemiluminescence (CL) in the presence of luminol and H 2 O 2 . Under optimal conditions, the CL intensity linearly increased with the concentration of the trigger DNA from 0.5 × 10 −6 to 1.0 × 10 −11 M and the detection limit is 7.0 × 10 −12 M. In this assay, three-input concatenated logic gates operating as a biocomputing keypad-lock security system also have been successfully constructed. When the inputs are all introduced the “lock” opens. This simple non L-HCR design realizes the fractal nanostructures construction, CL detection and logic gates operating. It can be used in the nanotechnology and biological assay.
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Fenglei Gao,Taotao Fan,Jing Wu,Sha Liu,Yan Du,Yao Yao,Fuyi Zhou,Yu Zhang,Xianjiu Liao,Deqin Geng
We describe a novel label-free and signal-on electrochemical DNA sensing platform via proximity hybridization triggered hemin/G-quadruplex formation based on the direct electron transfer of hemin . The thiolated modified G-DNA1 was first immobilized onto the Au electrode surface . In the presence of target DNA, Y-junction-structure ternary complex can be formed to trigger the proximity assembly of G-DNA1, hemin, and G-DNA2, which leads to the formation of hemin/G-quadruplex for generation an amplified electrochemical signal by differential pulse voltammetry . The observed signal gain was sufficient to achieve a demonstrated detection limit of 54 fM, with a wide linear dynamic range from 10 −13 to 10 −9 M and discriminated mismatched DNA from perfect matched target DNA with a high selectivity. Benefiting from the one step proximity dependent hemin/G-quadruplex formation, the assay method is extremely simple and can be carried out within 40 min. The advantages of free of any label conjugation step, and versatility make it a promising candidate for point-of-care testing and commercial application.
研究领域:
分析化学
·
生物化学
·
电化学
·
纳米技术
·
分子诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-9
·
IF 5.228
Liu Jing,Cui Meirong,Zhou Hong,Zhang Shusheng
A novel ternary composite of hemin-graphene-Au nanorods (H-RGO-Au NRs) with high electrocatalytic activity was synthesized by a simple method. And this ternary composite was firstly used in construction of electrochemiluminescence (ECL) immunosensor due to its double-quenching effect of quantum dots (QDs). Based on the high electrocatalytic activity of ternary complexes for the reduction of H 2 O 2 which acted as the coreactant of QDs-based ECL, as a result, the ECL intensity of QDs decreased. Besides, due to the ECL resonance energy transfer (ECL-RET) strategy between the large amount of Au nanorods (Au NRs) on the ternary composite surface and the CdS:Eu QDs, the ECL intensity of QDs was further quenched. Based on the double-quenching effect, a novel ultrasensitive ECL immunoassay method for detection of carcinoembryonic antigen (CEA) which is used as a model biomarker analyte was proposed. The designed immunoassay method showed a linear range from 0.01 pg mL −1 to 1.0 ng mL −1 with a detection limit of 0.01 pg mL −1 . The method showing low detection limit, good stability and acceptable fabrication reproducibility, provided a new approach for ECL immunoassay sensing and significant prospect for practical application.
研究领域:
分析化学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Lei Qin,Wenjing Chen,Yukui Fu,Jing Tang,Huan Yi,Ling Li,Fuhang Xu,Mingming Zhang,Weicheng Cao,Danlian Huang,Cui Lai
Heterogeneous electro-Fenton (HEF) technology has become a hot topic for degradation of organic pollutants in water. However, it remains a challenge to design effective catalysts for high H 2 O 2 utilization and pollutant degradation. In this work, we explored a novel iron and nitrogen-doped carbon catalyst derived from hemin (Fe-N-C) using KHCO 3 -MgO as dual-porogen. The results suggested that the KHCO 3 -MgO dual-porogen could not only improve the porous characteristics of catalyst, but also expose iron sites and change the proportion of nitrogen species. The pollutant degradation results demonstrated that the Fe-N-C-700/HEF system exhibited high ciprofloxacin degradation efficiency of 93.82% within 50 min and mineralization efficiency of 87.87% within 90 min by low energy consumption. Moreover, cycle experiments and metal ions leaching experiments revealed that the catalyst had good stability and recyclability. Electron spin resonance (ESR) test and radical capturing experiment showed that • OH was the dominant active species in this HEF system. In addition, the Fe-N-C-700/HEF system achieved satisfactory performance in the treatment of real water matrix, indicating the possibility for practical application in wastewater purification.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2023
·
IF 4.2
Wei Li,Huili Wang,Xinxin Ying,Zhen Liang,Jianna Li,Xiangjuan Chen,Lei Su,Xueji Zhang
Zr-metal–organic frameworks (Zr-MOFs) have received increasing interest for their use as the signal marker in the development of sandwich-structured aptasensors for the detection of exosomes. However, Zr4+ ions of the Zr-MOFs can interact with not only the exosomes, but also the aptamers, leading to possible false positives and a large background response. In the present study, we report for the first time aptasensors with Pd nanoparticle (NP)-decorated and hemin-embedded UiO-66 MOFs serving as the signal amplification marker to eliminate false positives and decrease the background response of aptasensors. To construct aptasensors for detection of exosomes, CD63-specific aptamers were tethered onto magnetic Fe3O4 particles coated with polydopamine (PDA) and UiO-66-NH2 using glutaraldehyde crosslinking for capturing the exosomes. To prepare highly catalytic Zr-MOF-based signal markers, UiO-66 MOFs were modified with hemin followed by Pd NPs. The as-prepared Pd-decorated hemin-embedded MOFs showed high catalytic activity towards the chromogenic oxidation reaction of TMB by H2O2. Moreover, the decoration with Pd NPs led to the change of the surface charge state of the catalytic hemin-embedded UiO-66 MOFs from positive to negative, weakening the interaction between the signal marker and the negatively charged aptamers. Therefore, the as-prepared aptasensors showed an improved sensing performance towards exosomes with a linear concentration range from 4.28 × 102 to 4.28 × 105 and a limit of detection (LOD) of 86.2 particles per μL. The as-prepared aptasensors also showed high sensitivity and selectivity to the exosomes from different origins including the HeLa cell line and MCF-7 cell line.
研究领域:
生物传感器
·
纳米技术
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）