BIOCHEMICAL JOURNAL
·
2021
·Vol. 478
·期 5
·1159-1173
·
IF 3.857
Shao Rong,Shi Jiahui,Du Kaili,Wang Na,Cai Weijie,Liu Siyu,Ding Zongxian,Wang Yihan,Li Dan
Abnormal lipid accumulation is associated to the development of metabolic diseases such as hepatic steatosis and lipid storage diseases. Pharmacological agents that can attenuate lipid accumulation therefore have therapeutic potentials for these diseases. Resveratrol (RSV), a natural active substance found in fruits and nuts, has been reported to effectively reduce the intracellular lipid accumulation, but the underlying mechanisms of RSV remain elusive. Here, we show that RSV triggers an endoplasmic reticulum (ER)- Ca2+ signaling that activates transcriptional factor EB (TFEB), a master transcriptional regulator of autophagic and lysosomal biogenesis. Moreover, RSV activates protein phosphatase 2A (PP2A), which binds and dephosphorylates TFEB, promoting its nuclear translocation and the expression of TFEB target genes required for autophagosome and lysosomal biogenesis. Notably, genetic inhibition of TFEB significantly ameliorates RSV-mediated lipid clearance. Taken together, these data link RSV-induced ER calcium signaling, PP2A and TFEB activation to promote autophagy and lysosomal function, by which RSV may trigger a cellular self-defense mechanism that effectively mitigate lipid accumulation commonly associated with many metabolic diseases.
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Molecular Medicine Reports
·
2015
·Vol. 13
·期 1
·224-230
·
IF 1.554
Hui Zhang,Qingfeng Sun,Tingyan Xu,Liang Hong,Rongquan Fu,Jinguo Wu,Jiguang Ding
Liver fibrosis is a wound-healing response to chronic liver injury that results in the accumulation of extracellular matrix proteins. It eventually leads to cirrhosis of the liver and liver failure, and it is a critical threat to the health and lives of patients with chronic liver diseases. No effective treatment is currently available. Resveratrol is a polyphenol with antioxidant, anti‑cancer and anti‑inflammatory properties. It has been reported that resveratrol prevents liver fibrosis, possibly by inhibiting NF‑κB activation. The present study investigated the mechanisms by which resveratrol prevented liver fibrosis, focusing on the possible involvement of the NF‑κB pathway. Mice with carbon tetrachloride (CCl4)‑induced liver fibrosis were treated with various concentrations of resveratrol. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and tumor necrosis factor (TNF)‑α were detected by ELISAs. Expression of α‑smooth muscle actin (α‑SMA), collagen I, inhibitor of NF‑κB (IκB) and NF‑κB were detected by western blot analysis. In addition, the present study examined the effects of resveratrol on the expression of fibrosis markers in LX‑2 cells. Western blot analysis was further used to detect the levels of Akt and phosphorylated Akt, as well as the nuclear levels of IκB, phosphorylated IκB and NF‑κB p65. The expression of α‑SMA in resveratrol‑treated LX‑2 cells was detected by immunofluorescence and flow cytometry, which demonstrated that resveratrol decreased the expression of α‑SMA in LX‑2 cells. Resveratrol also decreased CCl4‑induced upregulation of serum AST, ALT, TNF‑α, α‑SMA and collagen I. Finally, resveratrol prevented the activation of NF‑κB and Akt. The results of the present study therefore indicated that resveratrol attenuates liver fibrosis via the Akt/NF-κB pathways.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Nesma S. Abdelaty,Mostafa Gouda,Amel Thanina Amrouche,Cheng Chen,Baiyi Lu
Casein-based gel delivery systems often exhibit limited loading efficiency and functional performance under conventional formulation approaches. We present a novel UV-induced photo-crosslinking strategy for generating porous casein–polyethylene glycol gels, enhancing resveratrol stabilization and enabling controlled delivery during processing and storage. Structural characterization by FTIR and 13 C NMR supported the formation of an enhanced covalent network via ester and amide linkages. Formulation optimization showed that 10% casein achieved a crosslinking degree of 93.32 ± 0.43% ( p < 0.05), accompanied by rapid gelation (18 s) and a sixfold increase in storage modulus compared with the control, while gel electrophoresis profiles supported controlled degradation behavior. Resveratrol was efficiently encapsulated at 96.31 ± 1.40%, as evidenced by reduced fluorescence intensity in the cross-linked matrix, and the optimized gel retained 86% of the drug during storage. The optimized formulation exhibited enhanced antioxidant performance, with 27.03% higher DPPH radical-scavenging activity and 46.12% higher ABTS response compared to the non-crosslinked system, highlighting that structurally engineered casein-based gels can significantly enhance encapsulation efficiency and the controlled release of sensitive bioactives in pharmaceutical applications.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Haonan Huang,Haoyu Huang,Cheng Zuo,Jing Yang,Huizi Li,Fan Jiang,Ruibing Li,Lipeng Zhang,Siyu Rao,Fanrong Ai,Zhen Zong,Shengxun Mao
Peritoneal metastasis (PM) of colorectal cancer (CRC) remains a major therapeutic challenge due to the limited efficacy of current systemic and intraperitoneal treatments. To overcome these limitations, we developed an injectable, self-healing hydrogel constructed from a dual dynamic cross-linked network formed by borate ester and Schiff base bonds between phenylboronic acid-modified carboxymethyl chitosan (CMCS-PBA) and oxidized dextran (ODEX). This hydrogel was engineered for sustained intraperitoneal co-delivery of oxaliplatin (OXA) and resveratrol-loaded mesoporous silica nanoparticles (MSNs@RES). The system exhibited excellent biocompatibility and significantly inhibited cancer cell proliferation, migration, and invasion while inducing apoptosis and immunogenic cell death (ICD) in vitro. In a murine model of CRC peritoneal metastasis, the dual-drug-loaded hydrogel markedly suppressed tumor progression, reduced ascites formation, and prolonged survival. Proteomic analysis further revealed that treatment significantly modulated key signalling pathways, including HIF-1α-mediated angiogenesis, apoptosis-related cascades, and TGF-β-driven epithelial-mesenchymal transition (EMT). Collectively, this work presents a rationally designed localized delivery platform with significant potential for the treatment of colorectal cancer peritoneal metastasis.
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ACS Applied Materials & Interfaces
·
2026
·Vol. 18
·期 30
·40218-40231
·
IF 7.8
Xu Tianyu,Zhong Jun,Dai Chengbai,Du Yimin,Lyu Xinzhe,Ma Tian,Zhang Panpan,Zhu Xiaojia,Li Zheng,Quan Cheng,Hu Yuting
Osteoarthritis (OA) is a chronic degenerative disease causing bone and cartilage deterioration, joint deformity, and functional loss. The initiation and progression of OA are primarily driven by oxidative stress and the abnormal activation of the Wnt/β-catenin signaling pathway. This study involved loading Resveratrol (Res) and platinum (Pt) nanozymes, which exhibit superoxide dismutase (SOD) and catalase (CAT), onto a metal-organic framework (MOF) due to its distinctive porosity and high loading capacity. The system was further modified with hyaluronic acid (HA) to synthesize Pt-MOF/Res@HA (PMRH) nanoparticles. The PMRH nanoparticles improve early OA prevention and treatment by concurrently inhibiting the Wnt/β-catenin signaling pathway and eliminating reactive oxygen species (ROS). Experimental results, both in vitro and in vivo, indicate that PMRH nanoparticles efficiently scavenge ROS within the OA microenvironment. The PMRH nanoparticles inhibit the overactive Wnt/β-catenin signaling pathway in chondrocytes affected by osteoarthritis. This dual-target approach, which addresses oxidative stress and inhibits the Wnt/β-catenin signaling pathway, shows promising therapeutic potential for OA.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Pan Wang,Bangfeng Yin,Ping Zou,Zhenjia Zheng,Dan Wang,Peiyou Qin,Wenting Zhao,Yuanyuan Zhao,Ye Liu,Xiaoyan Zhao
To address the limitation of resveratrol (Res) related to its low bio-accessibility and poor stability, Res-pectin complexes with varying degrees of esterification were developed. The encapsulation efficiency of Res in these complexes was determined, revealing the following order: Res-L106 > Res-L13 > Res-H121 > Res-H115. Upon non-covalent interaction with pectins, Res exhibited enhanced stability, reduced crystallinity, and modified microstructure. The complexes exhibited stable particle sizes across a pH range of 4 to 8, with the Res-L106 complex showing minimal sensitivity to variations in ionic strength and temperature. All complexes exhibited improved antioxidant activities compared to pectins alone, with the Res-L106 complex displaying the highest activity. The complex enhancing the bio-accessibility of Res follows the order of Res-L106 > Res-L13 > Res-H121 > Res-H115. Additionally, all complexes promoted the production of short-chain fatty acids (SCFAs), with butyric acid being the predominant product. Notably, the Res-L106 complex exhibited the highest SCFAs-producing capacity. In conclusion, this study provides valuable insights into the design and optimization of strategies to enhance the bioavailability of Res, highlighting the potential of low-esterified pectin as an effective delivery carrier for bioactive compounds.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 3.1
Zhang Qiong,Yu Ying,Cheng Keyun,Deng Dan,He Shunv,Chen Chao,Wang Yingqian,Jin Danping,Shen Qing
Resveratrol (Res) has many potential benefits for human health. This investigation employed a complex protein system comprising pea protein isolate (PPI) and whey protein isolate (WPI) as an innovative carrier for Res delivery. The effect of PPI and WPI binding on protein conformation was investigated and differences in Res delivery by PW (pea protein isolate-whey protein isolate) complex protein and single PPI and WPI proteins were compared. The secondary structure and surface hydrophobicity results showed that the formation of PW complex protein increased the flexibility and surface hydrophobicity. Fluorescence spectroscopy results indicated that compared to single proteins, PW complex protein could bind more efficiently to Res through hydrophobic interactions. The PW-Res complex achieved higher encapsulation efficiency (EE), improving by 59.17% and 25.80% over PPI and WPI, respectively, and more significantly increased the antioxidant activity of Res by 36.89%. Furthermore, the smaller average particle size (173.95 ± 10.94 nm) and larger absolute value of ζ-potential (27.07 ± 0.49 mV) of the PW-Res complex resulted in better stability compared to the PPI-Res and WPI-Res complexes. This study has significant implication for encapsulation of hydrophobic active ingredients such as Res using complex protein systems.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zihao Wei,Duoduo Li,Yuqing Liu,Xiaolong Li,Changhu Xue,Jingyu Si,Wanjun Zhao
As a naturally occurring linear anionic polysaccharide, alginate has been extensively used as a coating in the construction of delivery systems for bioactive agents. It has been synergistically integrated with other biopolymers to engineer nanoscale carriers that are designed for the encapsulation and targeted delivery of specific active compounds. The aim of this study was to prepare resveratrol-loaded hollow gliadin/alginate nanoparticles (Res-loaded HGANPs). The results showed that the surface hydrophilicity and redispersibility of resveratrol-loaded hollow gliadin nanoparticles (Res-loaded HGNPs) was significantly enhanced by coating alginate on the surface of Res-loaded HGNPs through electrostatic interaction. Res-loaded HGANPs demonstrated superior hydrophilicity, pH stability, salt ion stability, storage stability, and light stability in comparison to Res-loaded HGNPs. In addition, the alginate coating could significantly inhibit the release of resveratrol in the stomach and promote its release in the small intestine. Therefore, the resveratrol encapsulated in hollow gliadin/alginate nanoparticles displayed better sustained release and intestinal targeting during in vitro simulated digestion process. Furthermore, Res-loaded HGANPs demonstrated higher encapsulation efficiency and superior stability retention in milk tea compared to Res-loaded HGNPs. In conclusion, alginate has significant potential in improving the stability and bioavailability of Res-loaded HGNPs. These results provided valuable insights into the development of Res-loaded HGANPs as a nutraceutical delivery system for resveratrol or other hydrophobic bioactives. These findings demonstrate the significant potential of alginate-coated hollow nanoparticles as functional ingredient delivery vehicles.
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ACS Omega
·
2024
·
IF 3.7
Pengyin Li,Shaoqing Chen,Yanyan Meng,Cheli Wang,Xinye Ni
Hydrogels have broad application prospects in bone repair. Pure poly(vinyl alcohol) (PVA) hydrogels have limited applications because of their low hardness and poor mechanical properties. This study found that resveratrol (Res) and PVA self-assembled and cross-linked through the formation of strong hydrogen bonds after freeze–thawing, forming an easily available PVA–Res supramolecular hydrogel through a green process. PVA–Res hydrogels with different Res wt %:10 wt % PVA ratios were prepared through freeze–thawing and designated as 0.4, 1.2, and 2.0 wt % PVA–Res hydrogels. Rheological studies demonstrated that the viscoelastic modulus of the PVA–Res hydrogels was significantly improved compared to pure PVA hydrogels. The viscoelastic modulus G′ of 1.2% PVA–Res hydrogel was 2299 Pa, which was 8.5-fold that of the pure PVA hydrogel. We conducted a study on cell proliferation and osteogenic differentiation using MC3T3-E1 (preosteoblasts from newborn mouse calvaria). The results showed that the 0.4% PVA-Res hydrogel promotes alkaline phosphatase activity and mineral deposition. Real-time quantitative PCR (RT-qPCR) analysis demonstrated that the 0.4% PVA–Res hydrogel upregulated the expression of osteogenic differentiation-related genes (BMP-9, OCN, and ALP). Furthermore, RT-qPCR and flow cytometry demonstrated that the 0.4% PVA–Res hydrogel could effectively promote the M2 transformation and polarization of mouse mononuclear macrophage leukemia cells (Raw 264.7). The expression of related genes, such as Arg-1 and CD206, significantly increased, whereas that of M1 polarization-related genes, such as iNOS and TNF-α, significantly decreased. In summary, PVA–Res supramolecular hydrogels are potential materials for use in bone repair.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Jiahuan He,Chunhong Zhang,Yingying Wang,Yuning Gong,Lanyue Zhang,Hao Liu,A. Yuhui,Lei Shang
Advancing bio-based non-isocyanate polyurethanes (NIPUs) is vital for fostering sustainable development. This study, inspired by the concept of "rigid and flexible" successfully utilized resveratrol , epoxidized soybean oil, and carbon dioxide (CO 2 ) to produce a novel type of NIPUs (CRSOP) with outstanding mechanical properties, recyclability, and adhesive properties . Test results reveal that CRSOP demonstrates a tensile strength of 20.85 MPa and an elongation at break surpassing 160 %. The synthesized CRSOP shows excellent recyclability, with a recycling efficiency of over 80 % even after three cycles. Additionally, the rich polar groups in CRSOP contribute to its exceptional adhesion on diverse substrates such as copper (5.29 MPa), steel (7.48 MPa), aluminum (6.12 MPa), glass (4.71 MPa), and polytetrafluoroethylene (1.75 MPa). Even after prolonged immersion in organic solvents , CRSOP maintains a lap shear strength comparable to the initial value, demonstrating outstanding environmental tolerance. This paper showcases a superior-performing NIPUs derived from renewable resources and simultaneously expands its extensive application in the adhesive field.
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GIANT
·
2026
·
IF 6.1
Yong Liu,Shi Hao Zheng,Fang Zhou,Hao Yuan
With an increasing focus worldwide on protecting the environment and developing in a sustainable way. Developing halogen-free, environmentally benign flame retardants is of critical relevance. To enhance the fire-safety performance of epoxy resins while simultaneously mitigating their environmental impacts. The bio-derived flame retardant DOPR was prepared in a single-step, one-pot process using resveratrol and DOPO as the key starting materials. The resulting product was subsequently incorporated into an epoxy matrix to obtain EP/DOPR composites. Despite encompassing only 0.35 wt % phosphorus, the EP/5DOPR composite exhibited an increased LOI of 28.19 % and secured the top-tier V-0 grade in the UL-94 flammability evaluation. Compared with neat EP, its pHRR, THR and TSP were lowered by nearly 15.65 %, 33.13 % and 9.62 %, respectively. Furthermore, the residual char yield of the composite was improved from 14.78 % to 17.88 %. The mechanical properties of the composite material were enhanced following the addition of 3wt % and 5wt % flame retardant, indicating a more favourable balance between flame retardancy efficiency and additive loading. The relevant research findings may provide a potential fundamental approach for the application of bio-based flame retardants to enhance the high performance of epoxy resins.
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MOLECULAR PHARMACEUTICS
·
2026
·
IF 4.9
Ning Yang,Yule Song,Junliang Song,Jian Qin,Mingwei Fu,Feng Yao,Huan Xu
Renal fibrosis (RF), a critical pathogenic mechanism in chronic kidney disease (CKD), now has exceedingly few therapeutic alternatives. This study presents a new sigma1 receptor (σ1R)-targeted nanodelivery system utilizing the natural polyphenolic medicine resveratrol (Res) as a therapeutic agent, addressing its low bioavailability and inadequate targeting properties, thereby providing a significant advancement in the treatment of RF. We engineered and produced a multifunctional material, aminoethyl anisamide-poly(2-ethyl-2-oxazoline)-cholesterol hemisuccinate (APC)-with benefits including active targeting, prolonged circulation, and pH sensitivity. In vitro investigations demonstrated that in the high glucose-induced HK-2 cell fibrosis model, the absorption efficiency of APC-modified liposomes was markedly enhanced, effectively suppressing the expression of fibrotic and inflammatory factors. In two typical animal models of RF, namely diabetic nephropathy and glomerulonephritis, APC@Res demonstrated therapeutic advantages, including modulation of the TGF-β1/Smad3 signaling pathway to impede fibrosis, a notable reduction in oxidative stress levels, enhanced renal function parameters, and diminished expression of inflammatory and fibrotic markers. This study innovatively employs the renal microacidic environment and sigma1 targeting to develop an effective delivery system, offering a novel treatment strategy for reversing RF.
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