ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Caihong Zhou,Yongzhao Zhang,Xuejing Yang,Ziqian Zhao,Menglei Xia,Chunhui Wei,Qiankun Wu,Zisong Chang,Long Ma,Lijuan Yin
Background Norovirus (NoV) is the leading cause of foodborne disease outbreaks worldwide, typically spreading via contaminated food and water. Rapid, sensitive, and portable detection of NoV is crucial. Results Here, we presented a magnetic CRISPR/Cas12a-SERS nanobiosensor capable of detecting NoV with high sensitivity, accuracy, speed, and portability. In this nanobiosensor, SERS nanoprobes linked to magnetic nanoprobes via linker single-stranded DNAs (ssDNAs). The presence of NoV nucleic acid triggered Cas12a's trans -cleavage activity, degrading the linker ssDNA. After magnetic separation, the dissociated SERS nanoprobes were efficiently separated from the magnetic nanoprobes. This enhanced the SERS signal in the supernatant, detectable using a portable Raman spectrometer. The detection limit for NoV is 100 copies/mL within 60 min. The nanobiosensor was further assessed in real-world settings, demonstrating excellent sensitivity and selectivity for detecting trace NoV in complex food samples. Significance This approach not only broadens CRISPR-based pathogen detection but also provides a reliable tool for monitoring foodborne viruses. Its potential extends beyond NoV, promising enhanced surveillance of various pathogens in food safety, environmental monitoring, and public health sectors.
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Jia Liu,Boning Zhang,Haifeng Yang,Ye Ying
Due to the small molecular weight and low Raman activity of γ-Aminobutyric acid (GABA), a Schiff base reaction was introduced. 4-mercaptobenzaldehyde (4-MBA) was assembled onto AuNPs via Au-S bond to form functional nanoparticles (AuNPs@4-MBA). Then, under certain conditions, it reacts with GABA to form a Schiff base, which increases the Raman scattering cross section area and enhances the Raman signal of GABA. In addition, because 4-MBA was assembled on AuNPs in advance through Au-S bond, the characteristic peak at 1080 cm −1 was selected as the internal standard, and compared with the characteristic peak at 1560 cm −1 of the newly generated C=N double bond. A linear standard curve was drawn between the peak intensity ratio of I 1560 /I 1080 and the concentration of GABA. The proposed material has a good linear relationship between 1 × 10 −5 and 1 × 10 −9 mol/L, R 2 was 0.9942, and the detection limit was 3.2 × 10 −11 mol/L. SERS detection of GABA was realized, and it was successfully applied to the detection of GABA in human serum that has important application value for future practical.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Deying Wang,Chunsheng Ren,Junfei Chen,Qi Zhang,Yan Chen,Changhao Li,Yong Jin,Xiaohua Qi,Qiang Ma
The reproducibility and reliability of surface-enhanced Raman spectroscopy (SERS) detection are constrained by the heterogeneous distribution of “hot spots”. While conventional physical deposition or template assembly methods can produce rigid and homogeneous substrates, they struggle to precisely regulate the self-assembly behavior of nanoparticles in flexible hydrogels. The core challenge lies in minimizing the variation in “hot spots” density across detection zones within hydrogels to enhance detection consistency and reliability of flexible SERS substrates. To address these issues, this study successfully constructed a flexible SERS substrate that integrates a high density of uniformly distributed SERS “hot spots” and environmental interference resistance. This was achieved by elucidating the synergistic matching mechanism between silver nanoflower morphology and laser spot size, combined with the flexible carrier properties of hydrogel chips. Using veterinary drug enrofloxacin in complex matrices as a model to validate the analytical performance of the SERS substrate, the results demonstrated significant advantages: its sensitivity reached 47.96 ppb, the entire detection process was completed within 20 min, and it effectively resisted background interference. In real milk sample validations, the substrate demonstrated spike recovery rates of 88.3%-114%, with relative standard deviations (RSD) controlled within 1.78%-7.83% across all concentration gradients. This work establishes a novel, sensitive, and reliable analytical platform for rapid screening of veterinary drug residues in food systems, holding significant application potential in food safety monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Lingxiang Bao,Ruizhi Yang,Lu Liu,Bin Zheng,Shan He,Miksusanti Miksusanti,Peiying Shi,Bin Zhang,Soottawat Benjakul,Yadong Zhao
Flavor nucleotides serve as key indicators for assessing the freshness of aquatic products. However, their rapid degradation over time poses a significant challenge for accurate detection, underscoring the need for rapid and highly sensitive analytical methods. In this study, a novel Au-Ag@Si surface-enhanced Raman scattering (SERS) sensor featuring a virus-like spike structure was fabricated by integrating physical vapor deposition and wet-chemical synthesis. Driven by a galvanic replacement reaction, well-ordered Au-Ag nanostructures were formed, which significantly enhanced the localized surface plasmon resonance through abundant contact sites and strong electromagnetic fields. This SERS platform was subsequently employed for the ultrasensitive and rapid detection of inosine monophosphate (IMP) and adenosine monophosphate (AMP), achieving limits of detection (LODs) as low as 1×10 -5 ppm and enhancement factors (EFs) of 2.33×10 4 and 3.22×10 4 , respectively. These results represent nearly an order of magnitude improvement in detection performance over conventional methods. The sensor was further validated in Procambarus clarkii using an internal standard method and simulated transport experiments. This study demonstrated that the virus-like Au-Ag@Si SERS sensor significantly enhances the detection of AMP and IMP, enabling rapid and ultrasensitive assessment of aquatic product freshness.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Xingkang Diao,GuoHua Qi,Yu Tian,Jing Li,Yongdong Jin
Hydrogen peroxide (H2O2) is a key reactive oxygen species (ROS) that regulates redox signals in organisms by reversibly oxidizing target proteins. However, it causes oxidative damage at supraphysiological concentrations, leading to various diseases. Therefore, accurate and sensitive detection of H2O2 in organisms, especially in cells, is essential for reliable pathological diagnosis. Herein, a sensitive and reproducible surface-enhanced Raman spectroscopy (SERS) platform was developed for in situ profiling detection of H2O2 released from cells utilizing a uniform and dense plasmonic Ag@Au core/shell nanoparticle nanomembrane (Ag@AuNPs-NM) as the SERS substrate. In the designed substrate configuration, the Ag@Au core/shell nanostructure combines the strong electromagnetic field enhancement of AgNPs with the stability of AuNPs, which could enhance the sensitivity of SERS detection. Meanwhile, the dense and uniform distribution of “hot spots” in the Ag@AuNPs-NM endows the platform with excellent detection reproducibility. In the presence of H2O2, the ratiometric I1073/I998 SERS signal of the Raman reporter 4-mercaptophenylboronic acid (4-MPBA) is changed due to the oxidation of the boronate group of 4-MPBA to the phenolic hydroxyl group of 4-hydroxythiophenol (4-HTP). The developed SERS sensing platform possesses a good linear relationship between the ratiometric I1073/I998 signal of 4-MPBA and the concentration of H2O2 in the range from 1 to 500 μM, with a limit of detection of 0.26 μM. As a proof of concept, we applied this platform to in situ monitoring of drug-stimulated H2O2 released from cells that were cultured on the SERS chips. This work provides a practical method for the in situ profiling detection of cellular H2O2 with satisfactory results, holding promise for the diagnosis of H2O2-associated diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
·
2017
·Vol. 49
·期 2
·245-255
·
IF 2.969
Xiu Liang,Xiao-Juan Zhang,Ting-Ting You,Nan Yang,Guang-Sheng Wang,Peng-Gang Yin
MoS 2 , a novel inorganic graphene analogues with typical two-dimensional structure, has attracted increasing interest in various fields. MoS 2 -based hybrid plasmonic structures have been extensively investigated as higher quality surface enhanced Raman scattering (SERS) substrates. Herein, we have designed a three-dimensional (3D) MoS 2 nanospheres (NSs) based hybrid nanostructure as SERS sensor by decorating Au nanoparticles (NPs) on the surface of 3D MoS 2 -NSs (3D MoS 2 -NS@Au-NPs). 3D MoS 2 -NSs acts as (a) a stable scaffold for Au NPs decoration via microwave-irradiation hydrothermal process, (b) a chemical mechanism source for Raman enhancement, and (c) a 3D spatial structure for generation of more effective hot spots due to Au coupling effect and supply larger surface area for adsorbing probe molecules. The 3D MoS 2 -NS@Au-NPs hybrid structure exhibits 56-fold improvement on enhancement factor compared to two-dimensional MoS 2 -NST@Au-NPs hybrid structures. SERS performance was easily and effectively optimized with the tuning of the density and sizes of Au NPs. The optimized 3D MoS 2 -NS@Au-NPs was applied as SERS sensor for melamine detection in milk. Quantitative detection was realized in the range of 1 ppb–10 ppm with a linear coefficient of .997. Excellent sensitivity of 1 ppb and uniformity of 10.12% relative standard deviation was achieved, which could meet the demand for melamine detection as prescribed by the U.S. Food and Drug Administration.
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Hongmei Li,Qing Wang,Ningning Gao,Jihong Fu,Xiaxia Yue,Xiaoyi Lv,FuRu Zhong,Jun Tang,Tao Wang
Developing a novel and simple route for the synthesis ultrasensitive and specific SERS substrates is highly desirable and technically important. Here, a magnetic ionic liquid (MIL) assisted strategy is developed, and the high-quality and ultra-sensitive MIL-gold nanoparticles/porous silicon (MIL-Au NPs/PSi) composite substrate were synthesized by a fast and cost effective strategy with the assistance of MIL (1-methyl-3-hexyl imidazole ferric tetrachloride ([C 6 mim]FeCl 4 ). Fundamental SERS performance of this composite substrate was evaluated using rhodamine 6G (R6G). The results showed that MIL make the Au NPs/PSi have great advantages in synergetic enhancement and stability, and the hypersensitive detection of specific substances can be realized by selecting or designing IL. Based on the particularity of MIL ([C 6 mim] FeCl 4 ′s function and structure, arsenic was detected with MIL-Au NPs/PSi substrate. The detection limit can reach 0.5 ppb, and the standard curves with regression coefficients of 0.98527 and 0.98965 were established in the linear range of 2–80 ppb and 200–800 ppb. In a word, an advanced method for the preparation of high-performance and designable SERS substrate has been developed, which will certainly show promising applications in detection technology.
✓ 已验证
DOI
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Yu Han,Xiaohui Fang,Zhoutao Sun,Chen Kang,Lei Zha,Xinping Zhang
Surface-enhanced Raman scattering (SERS) is a rapid and nondestructive spectroscopic method for trace detection. The enhancement based on both electromagnetic and chemical mechanism requires the molecules to be in close contact with the SERS-active material. Therefore, it is difficult to detect trace amounts of molecules in liquid directly. In this paper, graphene oxide (GO) and Ag nanoparticles (AgNPs) are uniformly modified on the inner wall of the capillary. GO can provide chemical enhancement by adsorbing the analyte onto its flat surface and producing efficient charge transfer resonance with the analyte. AgNPs can provide strong localized surface plasmon resonance for electromagnetic enhancement, while capillary can shorten the molecular enrichment time and provide long light–analyte interaction length. The synergism of GO, AgNPs, and capillary makes the optofluidic platform have the ability of ultrasensitive and real-time detection. The detection limit of Rhodamine 6G and thiram is as low as 10–12 and 10–10 M, respectively, and the enhancement factor is as high as 0.9 × 1010 and 108, which indicates that the SERS-active capillaries have great potentials in real-time ultratrace detection of water environment pollutants.
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Qiang Zou,Xinchen Ai,Tao Xue
In this work, an environment-friendly and label-free SERS probe was synthesized through Tollens' reagent and phytic acid to detect Fe 3+ ions in the integrated circuit cleaning solution waste. The synthesis process was greatly shortened to 30 min compared with the traditional synthesis time of 6 h. In addition, this SERS probe can be directly used and avoid the environmental pollution caused by the further modification in the synthesis process. This probe has a high sensitivity (LOD = 0.1 μmol L −1 ) and a wide detection range from 0.5 μmol L −1 to 500 μmol L −1 .As a practical application, it has been successfully applied to detect Fe 3+ ions in the standard clean two solution (SC-2). This method provides a feasible detection way for whether integrated circuit cleaning solution waste meets the emission criteria.
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Qinzhi Wang,Zhan Shi,Zhi Wang,Yijian Zhao,Jianuo Li,Huifan Hu,Yaowen Bai,Zhihao Xu,Hui Zhangsun,Li Wang
Acid orange II (AO II), a typical azo pigment, is strictly controlled by legislation and prohibited in foodstuffs. Herein, we prepared gold nanoparticles decorated amino-functionalized Cr-based metal-organic frameworks [NH 2 -MIL-101(Cr)@Au] via an in-situ reduction method as a surface-enhanced Raman scattering (SERS) substrate for simultaneous adsorption and detection of AO II. Gold nanoparticles are uniformly dispersed on the surface of NH 2 -MIL-101(Cr) owing to its three-dimensional (3D) structure and the interaction between –NH 2 and Au ions , providing more SERS-active “hot spots”. These NH 2 -MIL-101(Cr)@Au nanocomposites exhibited selective and high adsorption performance (419.85 mg g −1 ) for AO II, and could be used as superior SERS substrates for the detection of AO II with a low limit of detection (LOD) of 0.05 mg L −1 and wide detection range of 0.05–50 mg L −1 using portable Raman spectrometer. Furthermore, this SERS assay has been successfully used to determine AO II in orange juice and chili powder with good sensitivities.
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Ziqian Shi,Pengfei Wu,Hongyan Xi,Tingting You,Yukun Gao,Penggang Yin
Local surface plasmon resonance (LSPR) is a novel catalytic technique that has emerged in recent years, especially in the catalysis of aromatic amine compounds. However, the response process and mechanism are still unclear in current study. In the current field of study, the response process and mechanism are still unclear. In this work, the gas–liquid-solid three-phase interface (GLSTI) was innovatively utilized in this study to validate the reaction mechanism by surface-enhanced Raman spectroscopy. P-Aminothiophenol (PATP) and P-Phenylenediamine (PDA) underwent a surface plasmon-catalyzed reaction by using a silver nano-dendrites substrate with strong SERS activity. The GLSTI significantly facilitates the occurrence of surface plasmon catalytic reactions, which can supply enough oxygen by providing three-phase points. In situ SERS and EC-SERS technologies were combined in this study for the explorations. Therefore, this work is dedicated to deepening the exploration and expanding into new directions in plasmon-induced catalytic reactions.
✓ 已验证
DOI
高影响因子
Coatings
·
2025
·Vol. 15
·期 3
·267
·
IF 2.8
Junyu Liu,Hansheng Li,Wencan Cui,Shihua Sang,Liang Guan,Chen Zhao
This study focuses on the preparation of a highly active surface-enhanced Raman spectroscopy (SERS) substrate, where Ag nanoparticles synthesized by the Lee–Meisel method are loaded onto the surface of GO@PVP nanoparticles through classical electrostatic attraction. The substrate was characterized by SEM and XPS analysis. The SERS-active substrate was utilized for the detection of phenanthrene in aqueous solutions, achieving a detection limit of 10−8g/L (5.6 × 10−11mol/L). In comparison to conventional Raman spectroscopy, this method exhibits a significant reduction in the detection limit and holds promise for trace polycyclic aromatic hydrocarbons (PAHs) monitoring in environmental samples.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）