Advanced Science
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2025
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IF 14.1
Huilan He,Liang Liu,Yun Zheng,Jinlong Ji,Li Cao,Chunlian Ye,Yu Sun,Ying Zhang,Zhiyuan Zhong
The immunomodulation of the tumor microenvironment is critical for effective cancer immunotherapy, particularly for tumors that exhibit limited responses to conventional treatments. However, current immune agonists developed for tumor immunomodulation face several challenges, such as poor intratumoral retention, inadequate biocompatibility, and restricted cellular targets, which ultimately hamper their therapeutic efficacy and clinical application. In this study, a tumor-adhesive chitosan-tethered immune agonist construct (TACTIC) is introduced, which demonstrates good biocompatibility and robust immunostimulatory effects, enhancing the immunogenicity of tumor cells while simultaneously stimulating pro-inflammatory responses in various immune cell populations. Mechanistic investigations reveal that TACTIC targets multiple signaling pathways, conferring it to effectively remodel the irradiated tumor microenvironment, improve tumor control on murine cancer models post-radiotherapy, and elicit systemic immune responses with memory effects. The findings highlight the potential of TACTIC as a powerful macromolecular immune adjuvant, paving the way for its broader application in innovative cancer immunotherapies.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2024
·Vol. 416
·期 29
·6869-6877
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IF 3.8
Cui Xiaoqing,Geng Haoyuan,Guo Huanying,Wang Lei,Zhu Zihan,Zhang Yaqi,Chen Panpan,Wang Xiao,Sun Chenglong
Berberine (BR), an alkaloid isolated from the Chinese traditional medicine Coptidis rhizoma, exhibits therapeutic effects on several diseases including bacterial infections, diabetes, and hyperlipidemia, but the oral availability is poor. In this work, we prepared the chitosan microneedle array–loaded BR (BR-CS MNAs) to transdermally deliver BR, and the spatial distribution of BR in heterogeneous skin tissues was analyzed and imaged by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). Some endogenous phospholipids with specific spatial distribution were used to differentiate the epidermis and dermis regions of the skin. The results showed that BR was effectively delivered and could permeate to both epidermis and dermis regions of the skin. This demonstrated the feasibility of MALDI-MSI to evaluate the transdermal delivery efficiency of microneedle arrays and suggested BR could be transdermally delivered by CS MNAs. Graphical Abstract The chitosan microneedle array–loaded BR (BR-CS MNAs) was prepared and used to transdermal deliver BR. The spatial distribution of BR during the transdermal deliver process was analyzed and imaged by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). Some endogenous phospholipids with specific spatial distribution were used to help differentiate the epidermis and dermis regions of skin
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Can Wang,Jia-Jun Cui,Tao Sheng,Shuai Xia,Jia-Min Li,Bing-Feng Zhang
Oral nanoparticles coated with chitosan (CS) exhibited improvement of gastrointestinal therapeutics delivery and enhancement of liver fibrosis therapy. Here, chitosan-modified silibinin-hyaluronic acid nanoparticles (CS/HA-SLB NPs) was fabricated. The silibinin (SLB) was linked with hyaluronic acid (HA) by ester bond to from HA-SLB conjugates, and then self-assembled by dialysis to form silibinin-hyaluronic acid nanoparticles (HA-SLB NPs), finally coated with CS through electrostatic attraction. The prepared CS/HA-SLB NPs had a particle size about 220 nm, approximate spherical profile, positive potential and drug loading 3.71 % or so. The in vitro release of CS/HA-SLB NPs showed sustained release feature, and the NPs were stable in simulated gastric fluid with minimal release. The results of in vivo pharmacokinetic experiments showed CS/HA-SLB NPs had longer t l/2 and larger AUC than that of others group (Free SLB, HA-SLB NPs). Moreover, the intestinal loop absorption and in vivo drug distribution study exhibited that CS/HA-SLB NPs had better enhancement intestine absorption and liver targeting ability. In CCl 4 -induced liver fibrosis mice, CS/HA-SLB NPs group had a better anti-liver fibrosis effect than that of others group. Taken together, these findings suggested that CS-decorated silibinin prodrug self-assembled nanoparticles were expected to improve the bioavailability and anti-hepatic fibrosis effect of SLB.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Tangyu Yang,Haiwei Jia,Yuting Song,Dan Xu,Bing Li,Lin Li,Andre Skirtach,Xia Zhang
Enzyme-driven processes in green industries demand biocatalytic systems that harmonize efficiency, safety, and sustainability. Pickering interfacial biocatalysis (PIB) achieves impressive catalytic efficiency via emulsion-driven reactions, yet its potential is limited by unstable or harmful hydrophobic regulation. Herein, we present a novel synthesis strategy for biomaterials-based particles for PIB with high stability and sustainability through the immobilization of lipases within chitosan microgels enveloped by natural shellac nanoparticles (SNPs). The chitosan matrix establishes a confined microenvironment for lipase stabilization, while the SNPs provide hydrophobic regulation and physical barriers, enhancing Pickering emulsion stability and mitigating droplet coalescence. This system demonstrates high catalytic performance for hexyl hexanoate synthesis, achieving an 11-fold activity enhancement over free lipase in biphasic systems, alongside robust thermal/pH stability and reusability (95.10 % residual activity after 8 recycles and 69.25 % after 16 recycles). The high catalysis efficiency arises from huge interfacial surface area and accelerated interphase mass transfer endowed by Pickering emulsion. Physically embedded natural hydrophobic particles further provide this system with steady hydrophobic regulation and anti-coalescence properties. Crucially, given by the high biocompatibility of all components, this PIB system shows high promise in various applications, including food-grade enzymatic processing, cosmetics, and drug delivery areas.
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JOURNAL OF MATERIALS SCIENCE
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2025
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IF 3.9
Meng Xinyu,Wang Yikai,Yang Ming,Guo Xinglin,Wei Liuhe,Li Yuhan
Current marine antifouling materials predominantly utilize external biocides. However, this method is characterized by uncontrolled burst release and short-term efficacy, necessitating frequent reapplication and causing significant environmental pollution. To address these issues, we developed a more sustainable approach by leveraging the controlled release capabilities of microsphere materials. Specifically, temperature-sensitive chitosan molecules were grafted onto microsphere shells using ionic cross-linking, and the resulting microspheres were incorporated into a polyurethane coating. Given the complexity of the marine environment, with varying seawater temperatures and biological activities across different sea areas, this strategy aims to enhance antifouling effectiveness and sustainability. The microspheres achieved an encapsulation efficiency of 97.33% for copper pyrithione. The chitosan functionalization enabled temperature-dependent release, with a critical transition threshold near 20 °C. Incorporation of these microspheres into polyurethane coatings not only augmented mechanical toughness but also synergistically improved antifouling performance and interfacial adhesion. Notably, the microspheres exhibited superior antimicrobial performance compared to direct copper pyrithione application. This research underscores the potential for developing environmentally friendly materials with temperature-controlled antifouling agent release and introduces a novel approach for long-lasting antifouling strategies.
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CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Xue Zhang,Chunyi Luo,Na Liu,Siyu Liu,Zhouquan Jiang,Jinjun Zhou,Lishu Shao,Weihua Luo
To enhance the clinical value of carboxymethyl chitosan (CMCS) as an osteogenic material, improving its bioactivity and mechanical strength is crucial. Meanwhile, ellagic acid (EA) has strong bioactivities but low bioavailability due to its hydrophobicity. In this study, by introducing EA units into CMCS molecules, a near-infrared (NIR) light-mediated nanofiber membrane based on polycaprolactone (PCL)/EA-CMCS blended nanofibers coated with an EA-CMCS/graphene oxide (GO) layer (PCL/EA-CMCS@EA-CMCS/GO) was designed. The bioactivity of CMCS and bioavailability of EA could be significantly improved by covalent coupling of EA with CMCS. Blending EA-CMCS with PCL significantly enhanced the mechanical properties of nanofiber membranes. The coated nanofibrous membrane featured a multi-level structure of coating and blending. By leveraging the photothermal conversion effect of GO, it enabled the programmed and sustained release of EA-CMCS from both the coating and the nanofibers. Moreover, the membrane exhibited excellent biomineralization and antioxidant capabilities. It effectively regulated macrophage polarization and promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 osteoblasts. These properties enabled the membrane to regulate the harsh osteogenic immune microenvironment and promote bone regeneration. This biomimetic periosteum holds great potential as a substitute for periosteal grafts and offers a novel strategy for developing artificial periosteum.
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FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Yi-Lun Wang,Ruonan Li,Xiaoyu Yang,Liang Li
As single-function packaging increasingly fails to meet the demand for environmentally friendly convenience, multifunctional packaging is becoming increasingly important in food applications. In this study, inexpensive soybean protein is used as the raw material for membrane preparation, and it is fibrillated. Chitosan and Zinc oxide nanoparticle were added to it to improve its film-forming property, and a multifunctional film (SCZ) was made. SCZ exhibits high mechanical properties and water resistance. Furthermore, it displays exceptionally high antimicrobial activity (> 99%), antioxidant performance (> 80%), UV-blocking efficacy, and biocompatibility. Due to these multifunctional properties, SCZ has the potential to be used in food packaging applications, as demonstrated by tomato preservation experiments. It maintains good freshness both at room temperature and under refrigerator chilling conditions, and extends shelf life (25 °C, 6 d or 4 °C, 10 d). This research provides a new design concept for sustainable multifunctional food packaging materials.
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Innovative Food Science & Emerging Technologies
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2025
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IF 6.8
Chenchen Li,Xintong Zhang,Weiye Xiu,Mingfu Yuan,Jingyang Wang,Diaomei Ma,Chunran Han
Astaxanthin (AST) bioavailability is inherently limited by poor stability. In this study, pH/glutathione (GSH) dual-responsive carboxymethyl chitosan (CMCS) superparamagnetic iron oxide (SPION) nanoparticles were prepared, loaded with AST (CMCS-SPION-AST), and characterized. Its stability, responsive release, and kinetics were studied. The results showed that CMCS-SPION successfully encapsulated AST. The composite showed excellent stability (88.66 % AST retention at 4 °C, light-protected) and the highest cumulative release (82.11 % at pH 5.5/10 mM GSH), which was higher than the release rates of previously reported single pH-responsive carriers. The release curves of CMCS-SPION-AST at pH 5.5, pH 7.4/10 mM GSH, and pH 5.5/10 mM GSH showed good model fit (R 2 > 0.9), indicating non-Fickian diffusion. The release process represents a synergistic effect of diffusion and dissolution. This study provides novel insight for the development of responsive drug delivery systems and expands the application prospects of AST as a functional natural pigment in the food industry.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Ailong Hu,Zongwei Hao,Xinran Liu,Jinhua Song,Lihua Chu,Daxiang Li,Yiqun Du
Natural biopolymer-based nanoparticles are promising candidates for emulsion stabilization and delivery of hydrophobic compounds. In this study, tea protein (TP) and chitosan (CS) were employed to fabricate complex nanoparticles (NPs) using a pH-dependent method. At a mass ratio of 2:1 (TP:CS), the NPs exhibited favorable properties (206.62 nm, −30.86 mV, 85.41° contact angle) owing to electrostatic interactions, hydrogen bonding, and hydrophobic interactions (binding energy of the complex was −5.40 kcal/mol). At a particle concentration of 2 % and an oil-phase volume fraction of 60 %, the Pickering emulsion stabilized by TP-CS 2:1 NPs remained physically stable. Compared with TP alone, TP-CS 2:1 NPs significantly enhanced the encapsulation efficiency and antioxidant capacity (DPPH/ABTS) of curcumin (Cur). In vitro digestion confirmed that the emulsions reduced gastric degradation of Cur, increasing its bioaccessibility by 17.4 %. Accordingly, this study provides a strategy for designing natural protein-polysaccharide NPs for functional emulsions and sustained delivery systems.
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FOOD RESEARCH INTERNATIONAL
·
2025
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IF 8
Nan Cui,Yiyi Li,Haiyu Du,Liwen Liang,Yongqiang Wen,Zhu Zhu
The shelf-life of fruits and vegetables is significantly affected by atmospheric conditions and the microbial contaminations. The use of porous materials for packaging can help regulate the internal atmosphere within packaging. In this study, a chitosan-based film with enhanced gas permeability and antimicrobial properties was developed through the incorporation of polyethyleneimine (PEI)-modified mesoporous silica SBA-15. The mesoporous structure of SBA-15 enhances gas exchange, leading to superior permeability for oxygen, carbon dioxide, and water vapor compared to neat chitosan films. Additionally, the addition of polyethyleneimine (PEI) endows the composite film with remarkable antimicrobial efficacy, effectively inhibiting the growth of Escherichia coli , Staphylococcus aureus and Botrytis cinerea . The composite film also exhibited good transparency, UV-blocking capabilities, and mechanical strength. Preservation tests conducted on mangoes indicated that composite film was capable of regulating the internal atmosphere and delaying the deterioration of the fruit. Furthermore, the films exhibited excellent biodegradability and biocompatibility. Therefore, this composite film with enhanced gas transmission rate and antimicrobial properties presents considerable potential for the preservation of fresh produce.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
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IF 8.5
Manqi Yan,Menglu Huang,Xinyin Cao,Ziqi Zhou,Yangfeng Chen,Ye Zhang,Le Yin,Wei Zhao,Yong Hu
Strongly adhesive hydrogels show great promise for wound management yet frequently induce secondary tissue damage during dressing removal. To overcome this limitation, we designed a cationic hydrogel dressing with self-regulating adhesion using gelatin/carboxymethyl chitosan and MPTAC through UV-initiated crosslinking. The hydrogel achieved robust initial adhesion (8.39 ± 0.59 kPa) via electrostatic interactions with negatively charged skin surfaces, which progressively decreased to 2.21 ± 0.36 kPa over 7 days, enabling painless detachment. The material demonstrated a compressive strength exceeding 70 kPa, effective fluid absorption capacity, and potent antibacterial activity (>99 % against S. aureus ). It significantly accelerated hemostasis, reducing clotting time to 62.96 ± 3.95 s, and promoted wound healing in a full-thickness defect model, achieving 62 % wound closure within 7 days. These findings highlight the translational potential of this smart hydrogel as a next-generation wound dressing that combines secure wound protection with on-demand, trauma-free removal.
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ACS Applied Bio Materials
·
2019
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IF 0
Jiangying Zhu,Guohua Jiang,Gao Song,Tianqi Liu,Cong Cao,Yuhui Yang,Yajing Zhang,Wenjie Hong
Development of proper skin wound dressing is a vital step for wound repair, especially for those patients with serious skin injuries. Herein, zinc-doped bioactive glass (ZBG)/succinyl chitosan (SCS)/oxidized alginate (OAL) composite hydrogels (Gel-ZBG) have been developed as wound dressings to accelerate wound closure. Schiff-based linkages have been introduced into the composite hydrogels, which provide a humid microenvironment for the proliferation of cells on wound sites. The amino groups from SCS and Zn2+ released from ZBG exhibited excellent antibacterial properties to composite hydrogels, confirmed by the antibacterial tests in vitro. Si4+ and Ca2+ ions are essential factors that stimulate fibroblasts to secrete beneficial factors for angiogenesis and wound closure. The epidermal growth factor (EGF) was further embedded into the hydrogels to improve cell proliferation and tissue remodeling in the wound bed. Finally, the formation of granulation tissue, deposition of collagen and myofibril, the release of anti-inflammatory factors, and angiogenesis have been investigated to determine the healing mechanism of the composite hydrogels as the wound dressings.
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