BMC BIOTECHNOLOGY
·
2018
·Vol. 18
·期 1
·1-11
·
IF 2.605
Li Qi,Wu Tao,Qi Zhipeng,Zhao Linguo,Pei Jianjun,Tang Feng
Background β-D-xylosidase is a vital exoglycosidase with the ability to hydrolyze xylooligosaccharides to xylose and to biotransform some saponins by cleaving outer β-xylose. β-D-xylosidase is widely used as one of the xylanolytic enzymes in a diverse range of applications, such as fuel, food and the pharmaceutical industry; therefore, more and more studies have focused on the thermostable and xylose-tolerant β-D-xylosidases.Results A thermostable β-xylosidase gene (xln-DT) of 1509 bp was cloned from Dictyoglomus thermophilum and expressed in E.coli BL21. According to the amino acid and phylogeny analyses, the β-xylosidase Xln-DT is a novel β-xylosidase of the GH family 39. The recombinant β-xylosidase was purified, showing unique bands on SDS-PAGE, and had a protein molecular weight of 58.7 kDa. The β-xylosidase Xln-DT showed an optimal activity at pH 6.0 and 75 °C, with p-nitrophenyl-β-D-xylopyranoside (pNPX) as a substrate. Xln-DT displayed stability over a pH range of 4.0-7.5 for 24 h and displayed thermotolerance below 85 °C. The values of the kinetic parameters K
m
and V
max
for pNPX were 1.66 mM and 78.46 U/mg, respectively. In particular, Xln-DT displayed high tolerance to xylose, with 60% activity in the presence of 3 M xylose. Xln-DT showed significant effects on the hydrolyzation of xylobiose. After 3 h, all the xylobiose tested was degraded into xylose. Moreover, β-xylosidase Xln-DT had a high selectivity for cleaving the outer xylose moieties of natural saponins, such as notoginsenoside R1 and astragaloside IV, which produced the ginsenoside Rg1 with stronger anti-fatigue activity and produced cycloastragenol with stronger anti-aging activity, respectively.Conclusion This study provides a novel GH 39 β-xylosidase displaying extraordinary properties of highly catalytic activity at temperatures above 75 °C, remarkable hydrolyzing activity of xylooligosaccharides and rare saponins producing ability in the pharmaceutical and commercial industries.
研究领域:
生物化学
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酶学
·
生物技术
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DOI
PubMed
高影响因子
Polymers
·
2022
·Vol. 14
·期 10
·2002
·
IF 4.967
Chengyuan Xing,Xia Xu,Lei Song,Xiaoling Wang,Bangjing Li,Kun Guo
Poly (vinyl alcohol)/β-cyclodextrin (PVA/CD) composite fibers are prepared by wet spinning followed by hot stretching. XRD results show that β-CDs are in an amorphous state in fiber, and β-CD can help maintain the fibrous crystal that exists in the composite fiber. The DSC results show that the total crystalline ratio of the composite fibers decreased with the increase of β-CD. The as-prepared composite fibers were further crosslinked with glutaraldehyde (GA) to improve their usability. The crosslinked structure, together with amorphous β-CD, contributes to the loading and sustained release of fragrance molecules that were studied. The fragrance retention ratio of PVA/CD/GA is 55.63% and 48.25% forcis-jasmone and citronella, even after 25 days. The inclusion complexes of β-CD and fragrance molecules are confirmed by 2D-FTIR, which is responsible for the sustained release of fragrance. This study may contribute to the mass production of wearable long-term scented fabrics.Keywords:β-cyclodextrin;polyvinyl alcohol;wet spinning;functional fibers;fragrance retention
研究领域:
材料科学
·
纺织工程
·
化学
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DOI
PubMed
高影响因子
NUCLEAR MEDICINE AND BIOLOGY
·
2024
·
IF 3.6
Yuli Sun,Guangwen Li,Haiyan Hong,Lin Zhu,Hank F. Kung,Yan Zhang,Jinxia Zhu
Objective Diabetes mellitus (DM) is one of the major diseases in the world. Nuclear medicine imaging may be able to detect functional status of pancreatic β cells in vivo , which might elucidate the pathological mechanisms of diabetes and develop individualized treatment plans. In this study, we evaluated the ability of [ 125 I]ADAM, a serotonin transporter (SERT) imaging agent, as a probe for detecting pancreatic β-cell mass (BCM). Methods In vitro cell studies were evaluated in INS-1 cells (rat islet β cell line). Biodistribution studies were performed in male normal Sprague-Dawley rats and alloxan-induced type 1 diabetes mellitus (T1DM) rats. Distribution and expression of SERT protein in pancreas of rats were also measured by immunofluorescence staining and Western blot. Results In vitro cell studies showed that the concentration of [ 125 I]ADAM associated with the INS-1 cells was increased gradually with incubation time, and the SERT specific inhibitor, escitalopram, exhibited the inhibitory effect on this interaction. Biodistribution studies also showed that the uptake of [ 125 I]ADAM in the pancreas of normal rats was decreased in the presence of escitalopram. However, in the T1DM rat model with a significant β cells reduction, the uptake of pancreas was increased when compared with the control. Through immunofluorescence staining and Western blot, it was found that both the endocrine and exocrine cells of the normal pancreas expressed SERT protein, and the level of SERT protein in the exocrine cells was higher than islets. In the diabetic state, the expression of SERT in the exocrine cells was further increased. Conclusions The SERT imaging agent, [ 125 I]ADAM, at the present form will not be suitable for imaging β cells, specifically because there were extraordinarily high non-specific signals contributing from the exocrine cells of pancreas. In addition, we noticed that the level of SERT expression was abnormally elevated in the diabetic state, which might provide an unexpected target for studying the pathological mechanisms of diabetes.
研究领域:
医学影像学
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内分泌学
·
糖尿病研究
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DOI
高影响因子
Polymers
·
2024
·Vol. 16
·期 16
·2368
·
IF 4.7
Bukatuka Futila Clemence,Lin Xiao,Guang Yang
In this study, a novel oral formulation of berberine hydrochloride (BBH) hydrogel was successfully synthesized through physical cross-linking using chitosan (CS) and carboxymethyl-β-cyclodextrin (CMCD). The characterization results confirmed the successful synthesis of the CS/CMCD hydrogel and the subsequent loading of BBH into this composite (CS/CMCD/BBH) was effectively accomplished. The BBH was used as a model drug and the resulting hydrogel demonstrated a sustained drug release profile. In addition to its improved solubility and sustained release characteristics, the hydrogel exhibited excellent antibacterial activity against common pathogens such asEscherichia coli(E. coli),Staphylococcus aureus(S. aureus), andCandida albicans(C. albicans). Additionally, in vitro studies indicated that the hydrogel was not cytotoxic to NIH3T3 and HaCaT cells, suggesting its safety for biomedical applications. This lack of cytotoxic effects, combined with the mechanical strength, solubility improvements, and antibacterial properties of the hydrogel, positions the CS/CMCD/BBH hydrogel as a promising candidate for the effective oral delivery of BBH. By addressing the solubility and delivery challenges of BBH, this hydrogel offers a viable solution for the oral administration of BBH, with potential applications in various biomedical fields.
研究领域:
药剂学
·
生物材料学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PATHOLOGY
·
2022
·Vol. 258
·期 3
·213-226
·
IF 9.883
Zirong Lan,An Chen,Li Li,Yuanzhi Ye,Qingchun Liang,Qianqian Dong,Siyi Wang,Mingwei Fu,Yining Li,Xiaoyu Liu,Zhenyu Zhu,Jing-Song Ou,Xiaozhong Qiu,Lihe Lu,Jianyun Yan
Vascular calcification is an actively regulated process resembling bone formation and contributes to the cardiovascular morbidity and mortality of chronic kidney disease (CKD). However, an effective therapy for vascular calcification is still lacking. The ketone body β-hydroxybutyrate (BHB) has been demonstrated to have health-promoting effects including anti-inflammation and cardiovascular protective effects. However, whether BHB protects against vascular calcification in CKD remains unclear. In this study, Alizarin Red staining and calcium content assay showed that BHB reduced calcification of vascular smooth muscle cells (VSMCs) and arterial rings. Of note, compared with CKD patients without thoracic calcification, serum BHB levels were lower in CKD patients with thoracic calcification. Supplementation with 1,3-butanediol (1,3-B), the precursor of BHB, attenuated aortic calcification in CKD rats and VitD3-overloaded mice. Furthermore, RNA-seq analysis revealed that BHB downregulated HDAC9, which was further confirmed by RT-qPCR and western blot analysis. Both pharmacological inhibition and knockdown of HDAC9 attenuated calcification of human VSMCs, while overexpression of HDAC9 exacerbated calcification of VSMCs and aortic rings, indicating that HDAC9 promotes vascular calcification under CKD conditions. Of note, BHB treatment antagonized HDAC9-induced vascular calcification. In addition, HDAC9 overexpression activated the NF-κB signaling pathway and inhibition of NF-κB attenuated HDAC9-induced VSMC calcification, suggesting that HDAC9 promotes vascular calcification via activation of NF-κB. In conclusion, our study demonstrates that BHB supplementation inhibits vascular calcification in CKD via modulation of the HDAC9-dependent NF-κB signaling pathway. Moreover, we unveil a crucial mechanistic role of HDAC9 in vascular calcification under CKD conditions; thus, nutritional intervention or pharmacological approaches to enhance BHB levels could act as promising therapeutic strategies to target HDAC9 for the treatment of vascular calcification in CKD. © 2022 The Pathological Society of Great Britain and Ireland.
研究领域:
医学
·
分子生物学
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2013
·Vol. 169
·期 6
·1811-1821
·
IF 1.893
Wang Ting,Li Bin,Lin Li
A novel functional material of β -cyclodextrin ( β -CD) grafting cellulose beads containing immobilized silver nanoparticles (AgNPs) is presented in this paper. The morphology was characterized by scanning electron microscopy, energy-dispersive X-ray, and X-ray photoelectron spectroscopy. Phenolphthalein probe molecule technique was used to detect the activity of the grafting β -CD, and the results demonstrated that the deposition of AgNPs had no influence on its encapsulation ability. Acid resistance of the AgNPs on the bead material was studied by atomic absorption spectrometry. The stability of the AgNPs was enhanced due to the grafting of β -CD. Tube dilution method was applied to study the bacteriostatic effect, and the minimal inhibitory doses of the novel material against Escherichia coli and Staphylococcus aureus were 12.5 and 25 mg, respectively. The minimal bactericidal doses for the two bacteria were 25 and 25 mg, respectively.
研究领域:
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2023
·
IF 5.2
Chen Kai,Qin Ting,Pan Liangkun,Bing Xuwen,Xi Bingwen,Xie Jun
Aeromonas hydrophila is a significant pathogen to freshwater farmed animals, and antibiotics are usually used to control the bacterial septicemia caused by A. hydrophila. Due to the severe situation of development and spread of antibiotic resistance, there are stricter restrictions on antibiotics used in aquaculture. To evaluate the feasibility of glycyrrhetinic acid β (GA) as an alternative therapy against bacterial infection, in this study, an A. hydrophila isolated from diseased fish is used to test the antibacterial, anti-virulence activity and therapeutic effect of GA in vitro and in vivo, respectively. Results showed that GA did not affect the growth of A. hydrophila in vitro, while it could down-regulate (p < 0.05) the mRNA expression of the hemolysis-related genes hly and aerA, and significantly inhibited (p < 0.05) hemolytic activity of A. hydrophila. In addition, in vivo test showed that oral administration of GA was ineffective in controlling acute infections caused by A. hydrophila. In conclusion, these findings suggested that GA was a potential anti-virulence candidate against A. hydrophila, but the application of GA for the prevention and treatment of A. hydrophila-related diseases was still a long way.
研究领域:
微生物学
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水产养殖学
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兽医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
·
2015
·
IF 1.202
Yong H. Qi,Hui C. Zhang,Rui T. Xu,Jin Liu,Lei Zhang,Jian P. Wang
The objective of the present study was to develop a multi-analyte immunoassay for the determination of eight red dyes in food samples. Two dye intermediates (2-hydroxy-1-naphthoic acid and 1-amino-2-naphthol) were used as the haptens to produce the monoclonal antibodies. The obtained monoclonal antibodies recognized Sudan 1–4, Para red, Sudan red G, Sudan red B and Acid orange II simultaneously. After evaluation of different antibody/coating antigen combinations, a heterologous indirect competitive enzyme linked immunosorbent assay was developed to determine the eight red dyes in food samples (chili oil, chili powder, tomato sauce, hotpot seasoning). The crossreactivities to the eight analytes were in the range of 61%–79% (with β-naphthol as 100%), and the limits of detection were in the range of 1.3–1.9 ng/mL. The recoveries of the eight analytes from the fortified blank samples were in the range of 84.2%–115% with coefficients of variation lower than 18.3%. Therefore, this method could be used as a rapid and simple tool to detect the residues of the eight red dyes in foods.
研究领域:
分析化学
·
免疫学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Songlin Chen,Junchao Zhu,Chenlei Ma,Beibei Zhu,Huiyun Qiu,Shengqiang Tong
β- Cyclodextrin ( β -CD) exhibits exceptional chiral recognition ability, and covalent organic frameworks (COFs) have been reported as highly promising chiral carriers. Therefore, incorporating cyclodextrin into COFs may result in chiral covalent organic frameworks (CCOFs) suitable for chiral applications. In this work, a solvothermal method was employed to synthesize a classical porous covalent organic framework (COF-V), while β -CD underwent a two-step modification process, resulting in the synthesis of mercapto- β -cyclodextrin ( β -CD-SH). Subsequently, a β -CD-based covalent organic framework (COF-S-CD) was synthesized through a Thiol-Ene “click” reaction. The synthesized COF-S-CD was successfully applied in enantioselective liquid-liquid extraction for five racemic arylcarboxylic acids, including 2-phenylpropionic acid, 2-(4-methylphenyl) propionic acid, 2-(3-chlorophenyl) propionic acid, 2-(4-nitrophenyl) propionic acid and α -cyclohexylmandelic acid. Under optimized conditions, enantioseparation factors for the five racemates reached 1.78, 2.20, 1.84, 1.76 and 1.96, respectively, which indicated a significant enhancement in chiral recognition ability upon the incorporation of cyclodextrin into the COF material. This new application suggested that the synthesis of covalent organic framework materials incorporating β -CD, with the ability to uniformly disperse in the aqueous phase, holds promising potential for highly effective enantioselective liquid-liquid extraction.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 27
·3059-3067
·
IF 2.896
Guohui Li,Yafeng Wei,Lizhou Ma,Yin Mao,Ran Xun,Yu Deng
β-Conglycinin, composed of three subunits (α′, α and β), is the main allergen of soy protein which can cause severe allergic reactions, such as diarrhea, decreased growth performance and even death. Among them, the β subunit is more stable and difficult to remove, being one of the main nutritional inhibitors, which can be used to evaluate the concentration of β-conglycinin. However, there is no effective, accurate method for its β subunit rapid detection. Herein, we have successfully selected a high affinity β subunit aptamer (Kd = 6.9 nM) and developed a highly sensitive aptasensor. The aptasensor displayed high specificity and the β subunit at a concentration of 70–350 nM could be detected with a detection limit of 4.48 nM (3S/N). In addition, the recoveries of β subunit were more than 90%, demonstrating its practical properties for complicated conditions such as food quality control and disease diagnosis, without requiring expensive and sophisticated equipment.
研究领域:
生物化学
·
食品安全
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2017
·Vol. 64
·期 7
·795-803
·
IF 0.935
Junyu Lu,Lianqiang Wei,Dongmei Yao,Xiuju Yin,Hongfang Lai,Xiuxiang Huang
β-AgVO 3 nanorods have been demonstrated to exhibit intrinsic peroxidase-like activity. The oxidation of glucose can be catalyzed by glucose oxidase (GO x ) to generate H 2 O 2 in the presence of O 2 . The β-AgVO 3 nanorods can catalytically oxidize peroxidase substrates including o -phenylenediamine (OPD), 3,3′,5,5′-tetramethylbenzidine (TMB), and diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) by H 2 O 2 to produce typical color reactions: OPD from colorless to orange, TMB from colorless to blue, and ABTS from colorless to green. The catalyzed reaction by the β-AgVO 3 nanorods was found to follow the characteristic Michaelis–Menten kinetics. Compared with horseradish peroxidase and AgVO 3 nanobelts, β-AgVO 3 nanorods showed a higher affinity for TMB with a lower Michaelis–Menten constant ( K m ) value (0.04118 mM) at the optimal condition. Taking advantage of their high catalytic activity, the as-synthesized β-AgVO 3 nanorods were utilized to develop a colorimetric sensor for the determination of glucose. The linear range for glucose was 1.25–60 μM with the lower detection limit of 0.5 μM. The simple and sensitive GO x -β–AgVO 3 nanorods–TMB sensing system shows great promise for applications in the pharmaceutical, clinical, and biosensor detection of glucose.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Pharmaceutical Analysis
·
2013
·
IF 0
Nan Zhou,Xia-Shi Zhu
Ionic liquids functionalized β-cyclodextrin polymer, a mono-6-deoxy-6-(1,2-dimethylimidazolium)-β-cyclodextrin iodide polymer (ILs-β-CDCP), was synthesized as a solid-phase adsorbent coupled with high-performance liquid chromatography for separating or analyzing magnolol in drug samples. The results showed that magnolol was adsorbed rapidly on ILs-β-CDCP and eluted with methanol. Under the optimum conditions, preconcentration factor of the proposed method was 12. The linear range, limit of detection (LOD), correlation coefficient ( R ) and relative standard deviation (RSD) were found to be 0.02–8.00 μg/mL, 1.9 ng/mL, 0.9992 and 2.76% ( n =3, c =2.00 μg/mL), respectively. The interaction between ILs-β-CDCP and magnolol was studied through the inclusion constant, FTIR and TGA analysis. This proposed method has been successfully applied to the determination of magnolol in real samples.
研究领域:
分析化学
·
药物分析
✓ 已验证
产品关联
DOI
PubMed
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