ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
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IF 4.527
Kun Yan,Yanhua Liu,Qiulian Yang,Weixia Liu,Ruixin Guo,Jinhong Sui,Zhengyu Yan,Jianqiu Chen
Quantum dots (QDs), as a kind of novel nanomaterial , have the extensive applications in various fields, inevitably leading to increasing risks for the ecological environment. The mobilization of cadmium including metal smelting and subsequent machining for multifarious applications has caused the release of cadmium element into the environment. In this study, we evaluated the potential toxicity of a novel nanoparticle material CdSe QDs, using two green algae Chlorella pyrenoidosa and Scenedesmus obliquus . The impact of CdSe QDs and cadmium ions on algae and the sensitivity of the two algae on target compounds were also considered and compared. Our results showed the algal growth rates and chlorophyll content decreased with increasing exposure concentrations and durations. Moreover, the glutathione levels were decreased while the activities of superoxide dismutase increased, exhibiting their pivotal functions in defeating toxic stress. The increment of malondialdehyde levels revealed that the stresses of CdSe QDs and cadmium ions were contributed to the occurrence of oxidative damage. Our study also indicated that the impact of CdSe QDs was stronger than that of cadmium nitrate and the algal response was also species-specific. In addition, the TEM photographs of the algal ultrastructure showed the presence of surface attachment and uptake of QDs.
研究领域:
环境科学
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毒理学
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水生生物学
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纳米技术
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PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2020
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IF 6.793
Fang Wang,Bin Wang,Han Qu,Wenxing Zhao,Lei Duan,Yizhe Zhang,Yitong Zhou,Gang Yu
Plastic pollution has become a pressing issue due to its persistence in the environment. Smaller plastics are more easily ingested, potentially exerting greater influences on organisms. In this study, the effects of polystyrene nanoplastics (NP) on the toxic effects, bioaccumulation, biodegradation and enantioselectivity of ibuprofen (IBU) in algae Chlorella pyrenoidosa were explored. The influences on the growth rate, chlorophyll a , total antioxidant capacity (T-AOC), reactive oxygen species (ROS) and lipid peroxidation (MDA) were evaluated after 96 h of exposure to a combination of polystryene NP (1 mg L −1 ) and IBU (5–100 mg L −1 ). The results indicated that the inhibitory effect of IBU on C. pyrenoidosa growth was alleviated in the presence of NP. For instance, the 96 h-IC 50 value for rac-IBU in the treatment lacking NP was 45.7 mg L −1 , and the corresponding value in the treatment containing NP was 63.9 mg L −1 . The co-exposure of NP led to a significant enhancement of T-AOC and slight reduction of ROS and MDA compared with the individual exposure (IBU) group, suggesting a decreased oxidative stress . In addition, treatment with NP led to a decreased bioaccumulation and accelerated biodegradation of IBU in C. pyrenoidosa and enhanced removal in the medium. The enantioselective toxicity, bioaccumulation and biodegradation of IBU were observed both in the absence and presence of NP. S-IBU exhibited a greater toxicity, and R-IBU was preferentially accumulated and degraded in C. pyrenoidosa . No interconversion of the two enantiomers occurred regardless of the presence of NP. This consequence implied that the influence of coexistent NP should be considered in the environmental risk assessment of pharmaceuticals and personal care products in aquatic environments.
研究领域:
环境科学
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毒理学
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生态学
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DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2022
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IF 9.988
Wei Song,Caixia Fu,Yuning Fang,Zhuoyue Wang,Ji Li,Xiaolei Zhang,Kalpana Bhatt,Lu Liu,Ningjie Wang,Fang Liu,Shunni Zhu
Microplastics (MPs), an emerging pollutant, have been increasingly raising concern due to the potential impacts on aquatic organisms. Moreover, the environmental aged MPs always exhibit different environmental behavior and interaction effect with organic pollutants from virgin MPs. In this work, the single and combined toxicity impact on Chlorella pyrenoidosa , a symbiont representative, has been investigated between MPs (e.g., polyamide microplastic (PA6), 75 μm) and organic pollutants (e.g., sulfamethoxazole (SMX) and dicamba (DCB)). Growth inhibition, chlorophyll accumulation, superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) were investigated with the primary or UV-aged PA6. Above 0.5 g/L PA6 (primary or UV-aged) inhibited cell growth and chlorophyll accumulation after 96 h cultivation as compared with the control. Besides, the inhibition impacts have enhanced as the UV-aging time extending in the single PA6 systems. The algae growth inhibition rate after 96 h cultivation in both the system i.e., single (PA6: 6.9%) and combined (PA6-SMX: 14.2%, PA6-DCB: 14.9%) was slightly lower than that of exposing in organic pollutants alone (SMX: 23.9%, DCB: 25.0%), while the chl. b concentration in 60 days UV-aged PA6 combined with SMX (1.19 mg/L) or DCB (1.40 mg/L) systems were higher than in single SMX (1.04 mg/L) or DCB (1.33 mg/L) system. In addition, there were several differences of the cellular oxidative stress in the combined system of SMX and DCB. Specially, it was not noticeable of three enzymatic activities for SMX exposing in the presence of primary or UV-aged PA6. While SOD, CAT, and MDA activities was obviously increasing after exposing in PA6 and DCB combined system, indicating the significant synergistic effect on algae cells damage. This research verified the remarkable combined toxicity between UV-aged MPs and organic pollutants on microalgae.
研究领域:
环境科学
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毒理学
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DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2021
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IF 4.401
Pingkang Qian,Kun Gao,Zheng Gao,Chunye Xue,Xiaoli Hu,Qiaoqiao Liu,Jingwei Dong,Xiangyuan Deng
The aim of this work was to investigate the effects of myo -inositol (MI) on growth, photosynthetic activity , and chemical compositions of Chlorella sorokiniana . Results showed that a maximum value of cell density was obtained when this alga was cultivated in BG11 media with 1000 mg L −1 MI, which was 1.37 times greater than that in the controls. Moreover, it was observed that MI had a negative effect on algal photosynthetic activity, which could be alleviated by regulating the contents of chemical compositions, such as chlorophyll a (Chl a ). In the BG11 media with MI, contents of Chl a in the algal cells were in the range of 0.30–0.35 μg 10 −6 cells, which were about 1.75 times greater than that in the controls during microalgae cultivation. The accumulated Chl a would be used to capture and deliver more photons during algal photosynthesis . In addition, contents of lipids , carbohydrates, and proteins varied with the changes in MI concentrations. Thus, it is concluded that MI could promote the algal growth and play an important role in regulating the biochemical compositions in algal cells. These data would provide basic theory and technical support for large-scale cultivation and biomass production of microalgae by adding MI to culture media.
研究领域:
水生生物学
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生物化学
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高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2023
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IF 8.7
Dongyang Liu,Hanzhi Wang,Yue Teng,Qirui Wu,Chunming Tang,Xinxin Gao,Chaoqi Chen,Liandong Zhu
With the development of livestock industry , contaminants such as divalent zinc ions (Zn (Ⅱ)) and estrone are often simultaneously detected in livestock wastewater. Nevertheless, the combined toxicity of these two pollutants on microalgae is still unclear. Moreover, microalgae have the potential for biosorption and bioaccumulation of heavy metals and organic compounds. Thus, this study investigated the joint effects of Zn (Ⅱ) and estrone on microalgae Chlorella sorokiniana , in terms of growth, photosynthetic activity and biomolecules , as well as pollutants removal by algae. Interestingly, a low Zn (Ⅱ) concentration promoted C . sorokiniana growth and photosynthetic activity, while the high concentration experienced inhibition. As the increase of estrone concentration, chlorophyll a content increased continuously to resist the environmental stress. Concurrently, the secretion of extracellular polysaccharides and proteins by algae increased with exposure to Zn (Ⅱ) and estrone, reducing toxicity of pollutants to microalgae. Reactive oxygen species and superoxide dismutase activity increased as the increase of pollutant concentration after 96 h cultivation, but high pollutant concentrations resulted in damage of cells, as proved by increased MDA content. Additionally, C. sorokiniana displayed remarkable removal efficiency for Zn (Ⅱ) and estrone, reaching up to 86.14% and 84.96% respectively. The study provides insights into the biochemical responses of microalgae to pollutants and highlights the potential of microalgae in pollutants removal.
研究领域:
环境科学
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生物化学
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PubMed
高影响因子
Environmental Science-Processes & Impacts
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2023
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IF 5.5
Yi Liu,Mengen Kang,Yuzhu Weng,Yuanyuan Ding,Xue Bai
Recent studies have reported that nanoparticles (NPs) released into the aquatic environment may interact with persistent organic pollutants such as brominated flame retardants, whereas the environmental processes and toxicological impacts induced by such binary NPs require further specification. This study investigated the ultrastructural damage of Chlorella vulgaris triggered by exposure to zinc oxide (ZnO) NPs, tetrabromobisphenol A (TBBPA), ZnO–TBBPA, and ZnO–TBBPA–humic acid (HA), clarified the uptake and distribution of ZnO NPs in cells, and explored the physiological toxicity and tolerance mechanism. The results demonstrated that ZnO NPs induced irregular morphology in algal cells, and the disruption of the cellular ultrastructure by binary ZnO–TBBPA was also extremely severe due to the excessive uptake of ZnO NPs, which resulted in strong oxidative stress responses. In particular, the accumulation of reactive oxygen species further exacerbated the reduction of total chlorophyll content and algal density. Moreover, the cluster heat map and correlation analysis revealed that superoxide dismutase activity played a critical role in alleviating lipid peroxidation damage and enhancing the tolerance of algal cells to the stress of binary ZnO NPs. More notably, the existence of HA intensified the dispersion stability of NP suspensions and significantly mitigated the synergistic toxicity of binary ZnO–TBBPA. This study provides new insights into the environmental behavior and biological impacts of binary NPs in the natural environment.
研究领域:
环境科学
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毒理学
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植物学
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PubMed
高影响因子
CHEMOSPHERE
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2019
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IF 5.108
Han Zhang,Na Liu,Jinfeng Zhao,Fei Ge,Yin Xu,Yuehui Chen
Oxygen evolution complex (OEC) in photosystem II (PSII) is sensitive to environmental stressors. However, oxidative damage mechanism in PSII-OEC is still unclear. Here, we investigated photosynthetic performance of PSII, oxidative stress and antioxidant reaction induced by reactive oxygen species (ROS) in a unicellular green alga Chlorella vulgaris ( C. vulgaris ) under the stress of cetyltrimethylammonium chloride (CTAC). From the changes of chlorophyll fluorescence parameters and PSII activity, it was proved that the electron transport, which occurred initially at the electron donor side of OEC, was disturbed by CTAC. Moreover, a significant decrease of the oxygen evolution rate in OEC (40.95%) while an increase of ROS (50.50%) was obtained after the exposure to 0.6 mg/L CTAC compared to the control (without CTAC), confirming that more oxygen transferred to ROS under the stress. Furthermore, the increased ROS in chloroplast and the structural destruction in thylakoid membrane were observed, respectively. These results proved that oxidative damage mechanism in PSII-OEC is mainly through the reduction of oxygen evolution and the production of excessive ROS, thus leading to the destruction of OEC performance and chloroplast structure.
研究领域:
植物生理学
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环境毒理学
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藻类生物学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Qingxia Yuan,Rongyi Liang,Kunling Lv,Xiaohuo Shi,Jing Leng,Yonghong Liu,Jian Xiao,Lifeng Zhang,Longyan Zhao
Chlorella polysaccharides have been gaining increasing attention because of their high yield from dried Chlorella powder and their remarkable immunomodulatory activity. In this study, the major polysaccharide fraction, CPP-3a, in Chlorella pyrenoidosa , was isolated, and its detailed structure was investigated by analyzing the low-molecular-weight product prepared via free radical depolymerization. The results indicated that CPP-3a with a molecular weight of 195.2 kDa was formed by →2)-α-L-Ara f -(1→, →2)-α-D-Rha p -(1→, →5)-α-L-Ara f -(1→, →3)-β-D-Glc p- (1→, →4)-α-D-Glc p- (1→, →4)-α-D-Glc p A-(1→, →2,3)-α-D-Man p- (1→, →3,4)-α-D-Man p- (1→, →3,4)-β-D-Gal p- (1→, →3,6)-β-D-Gal p- (1→, and →2,3,6)-α-D-Gal p- (1→ residues, branched at C2, C3, C4, or C6 of α/β-D-Gal p and α-D-Man p , and terminated by α/β-L-Ara f , α-L-Ara p , α-D-Gal p , and β-D-Glc p . Biological assays showed that CPP-3a significantly altered the dendritic morphology of immature dendritic cells (DCs). Enhanced CD80, CD86, and MHC I expression on the cell surface and decreased phagocytic ability indicated that CPP-3a could induce the maturation of DCs. Furthermore, CPP-3a-stimulated DCs not only stimulated the proliferation of allogeneic naïve CD4 + T cells and the secretion of IFN-γ, but also directly stimulated the activation and proliferation of CD8 + T cells through cross-antigen presentation. These findings indicate that CPP-3a can promote human DC maturation and T-cell stimulation and may be a novel DC maturation inducer with potential developmental value in DC immunotherapy.
研究领域:
免疫学
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生物化学
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药理学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2026
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IF 8.2
Yuhao Yan,Hong Gao,Rundong Chen,Zhigang Wang,Cheng Zeng,Jia Yuan,Jianjun Liu,Rong Chen
Triazine-based UV filters exhibit potential low toxicity risk and efficient biodegradation. • OCR causes irreversible damage and should be treated as a priority pollutant. • DHHB persistently inhibits photosynthesis, requiring assessment of chronic low‑dose risks. • AVB exhibits adaptation but poses cumulative risk under environmental fluctuation. Low-dose chronic toxicity can induce sublethal biological effects, which is of great significance for assessing the ecological toxicity of UV filters. In this study, we investigated the chronic toxicity of three triazine-based UV filters including bis-ethylhexyloxyphenol methoxyphenyl triazine (BEMT), diethylhexyl butamido triazone (DBT), and ethylhexyl triazone (EHT) and three non-triazine UV filters: diethylamino hydroxybenzoyl hexyl benzoate (DHHB), avobenzone (AVB), and octocrylene (OCR) on Chlorella vulgaris . Results indicated that the triazine-based UV filters exerted no statistically significant effects (p > 0.05) on Chlorella vulgaris cell growth, photosynthetic pigment-contents, superoxide dismutase (SOD) and catalase (CAT) activities, or malondialdehyde (MDA) content throughout the entire exposure period (p > 0.05). Consequently, these compounds triggered no discernible antioxidant response in Chlorella vulgaris , implying that the exposure regimes were inadequate to induce measurable physiological alterations. By contrast, the three non–triazine UV filters exhibited markedly different chronic toxic effects (p < 0.05). The Chlorella vulgaris in the AVB treatment group exhibited a dynamic toxicity profile and induced reversible, adaptive oxidative stress. DHHB displayed sustained growth inhibition throughout the exposure period and primarily impairs Chlorella vulgaris growth via direct phototoxicity targeting the photosynthetic apparatus. While OCR manifested delayed toxicity, driven by irreversible, intense oxidative damage. Further investigations revealed that triazine–based UV filters could be efficiently biodegraded by Chlorella vulgaris , with removal efficiencies reaching 85 %. Collectively, these results demonstrate that triazine–based UV filters possess greater ecological safety than non–triazine counterparts. This study thus provides critical foundational data for the ecological risk assessment of emerging triazine-based UV filters. Download: Download high-res image (177KB) Download: Download full-size image
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
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IF 5.9
Chunguang Ren,Shuling Zhuang,Tianyu Zhao,Wenshuo Shang,Zhihao Meng,Jiaxin Liu,Yuhang Ren,Xue Jiang,Lingjie Meng,Hong He,Xinyan Gao,Zhonghua Wang,Jingwei Tian,Linlin Wang
Tissue hypoxia and bacterial infections are capable of impeding the wound healing process, imposing a significant burden on patients and healthcare systems. Although significant progress has been gained in wound care, traditional dressings are deficient in therapeutic effects. Furthermore, their inability to fit irregular wound geometries and the absence of self-healing properties can compromise the healing process. Inspired by the environmental adaptability and biological plasticity of amoebas, we developed an adaptive hydrogel (PLC hydrogel) with oxygen-releasing and antibacterial activities by loading Chlorella vulgaris and ampicillin based on a matrix composed of PVA and ι-Carrageenan. The hydrogel integrated moldability, adaptability, self-healing ability, and injectability. By incorporating Chlorella and ampicillin, the PLC hydrogel can continuously generate oxygen and inhibit bacterial infections. The hydrogel possessed excellent electrical and sensing output properties, which also exhibited hemostatic capacity and good biocompatibility. Notably, results from in vivo wound healing evaluations indicated that the PLC hydrogel exerted a positive effect on accelerating wound healing. These results highlight the potential of the multifunctional hydrogel in the therapeutic health management of wounds at active sites, as well as in personalized and precise therapeutic diagnosis, thereby providing new insights for the research and development of novel wound dressings.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Jiaru Ma,Qian Xiong,Fei Tian,Hengyu Wu,Fengqi Xie,Yanfen Chen,Qinglin Tang,Yuqin Zou,Xinyan Huang,Yousheng Liu,Guangguo Ying
Benzotriazoles (BTRs), widely used as tire additives, pose a significant challenge as persistent aquatic pollutants due to their recalcitrance to conventional biological degradation. This study elucidated methanol (ME)-enhanced co-metabolic degradation of 1H-benzotriazole (BT) and 5-chlorobenzotriazole (CBT) by green microalgae. Among the six microalgal species evaluated, Chlorella pyrenoidosa demonstrated superior removal of BT and CBT under 1 % ME supplementation, achieving efficiencies of 33.50 % and 89.67 %, respectively. ME functioned as an electron donor, which favored the reductive dechlorination of CBT, leading to a 2.85-fold higher removal efficiency compared to BT. Biotransformation was identified as the dominant removal mechanism, with 11 and 10 transformation products (TPs) detected for BT and CBT, respectively. An integrated analysis of TPs and transcriptomic data revealed that ME activates a metabolic enzyme-photosynthetic machinery, enhancing electron transport, energy generation, cytochrome P450 (CYP450) expression, and extracellular polymeric substance (EPS) production, thereby facilitating efficient pollutant removal. The proposed degradation pathways further confirmed the role of ME in facilitating specific biotransformation reactions. These findings provide mechanistic insights and establish a theoretical foundation for the development of ME-microalgae co-metabolism systems as a sustainable technology for remediating persistent pollutants in aquatic ecosystems.
研究领域:
环境科学
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藻类学
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微藻生物技术
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污染物修复
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水生毒理学
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代谢途径
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PubMed
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
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2025
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IF 7.8
Lei Hu,Yuxuan Tian,Ning Ding,Hong Liu
In this study, iron-doped molybdenum disulfide (FeMoS 2 ) was hydrothermally synthesized and subsequently coupled with calcined graphitic carbon nitride (g-C 3 N 4 ) to construct a heterojunction . Comprehensive characterization confirmed the stable crystalline structure of the composites and the formation of robust interfacial coupling, which facilitated efficient photogenerated charge separation. Furthermore, UV-Vis diffuse reflectance spectroscopy revealed a significant extension of the visible-light absorption edge compared to pristine FeMoS 2 , with the bandgap narrowing to 1.99 eV. Under optimized conditions (0.6 g/L catalyst dosage, 1.0 mM peroxydisulfate (PDS), pH 6), the FeMoS 2 /g-C 3 N 4 /PDS system achieved 88 % Chlorella vulgaris inactivation in 60 min under visible light, retaining more than 60 % efficiency after five cycles. Mechanistic investigations revealed that superoxide radicals (·O 2 - ) and photogenerated holes (h + ) synergistically inactivated Chlorella via reactive oxygen species (ROS)-induced membrane peroxidation and antioxidant system disruption. Algal physiological assays and microscopic imaging further corroborated that photocatalytically generated ROS compromised cellular membrane integrity and induced chloroplast degradation.
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高影响因子
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