JOURNAL OF NANOBIOTECHNOLOGY
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2023
·Vol. 21
·期 1
·1-15
·
IF 10.2
Pang Xin,Xu Haohang,Geng Qishun,Han Yu,Zhang Huiya,Liu Heng,Zhang Xiao,Miao Mingsan
Effective diagnosis and therapy for bacterial infections, especially those caused by multidrug-resistant (MDR) species, greatly challenge current antimicrobial stewardship. Monocytes, which can chemotactically migrate from the blood to infection site and elicit a robust infection infiltration, provide a golden opportunity for bacterial theranostics. Here, a nano-Trojan Horse was facilely engineered using mannose-functionalized manganese-eumelanin coordination nanoparticles (denoted as MP-MENP) for precise two-step localization and potent photothermal-immunotherapy of MDR bacterial infection. Taking advantage of the selective recognition between mannose and inflammation-associated monocytes, the MP-MENP could be passively piggybacked to infection site by circulating monocytes, and also actively target infiltrated monocytes that are already accumulated in infection microenvironment. Such dual-pronged targeting enabled an efficient imaging diagnosis of bacterial infection. Upon laser irradiation, the MP-MENP robustly produced local hyperemia to ablate bacteria, both extracellularly and intracellularly. Further combined with photothermal therapy-induced immunogenic cell death and MP-MENP-mediated macrophage reprogramming, the immunosuppressive infection microenvironment was significantly relieved, allowing an enhanced antibacterial immunity. Collectively, the proposed nanotheranostic Trojan Horse, which integrates dual-pronged targeting, precise imaging diagnosis, and high-performance photothermal immunotherapy, promises a new way for complete eradication of MDR bacterial infection.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-8
·
IF 16.6
Lu Yahui,Chen Xing,Han Fang,Zhao Qian,Xie Tao,Wu Jingjun,Zhang Yuhua
3D printing can produce intuitive, precise, and personalized anatomical models, providing invaluable support for precision medicine, particularly in areas like surgical training and preoperative planning. However, conventional 3D printed models are often significantly more rigid than human organs and cannot undergo repetitive resection, which severely restricts their clinical value. Here we report the stereolithographic 3D printing of personalized liver models based on physically crosslinked self-healing elastomers with liver-like softness. Benefiting from the short printing time, the highly individualized models can be fabricated immediately following enhanced CT examination. Leveraging the high-efficiency self-healing performance, these models support repetitive resection for optimal trace through a trial-and-error approach. At the preliminary explorative clinical trial (NCT06006338), a total of 5 participants are included for preoperative planning. The primary outcomes indicate that the negative surgery margins are achieved and the unforeseen injuries of vital vascular structures are avoided. The 3D printing of liver models can enhance the safety of hepatic surgery, demonstrating promising application value in clinical practice.
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ACTA PHARMACOLOGICA SINICA
·
2023
·
IF 8.2
Wang Meng-yang,Yu Tian-xiang,Wang Qin-yan,Han Xue,Hu Xiang,Ye Shi-ju,Long Xiao-hong,Wang Yi,Zhu Hong,Luo Wu,Liang Guang
Hypertensive renal disease (HRD) contributes to the progression of kidney dysfunction and ultimately leads to end-stage renal disease. Understanding the mechanisms underlying HRD is critical for the development of therapeutic strategies. Deubiquitinating enzymes (DUBs) have been recently highlighted in renal pathophysiology. In this study, we investigated the role of a DUB, OTU Domain-Containing Protein 1 (OTUD1), in HRD models. HRD was induced in wild-type or Otud1 knockout mice by chronic infusion of angiotensin II (Ang II, 1 μg/kg per min) through a micro-osmotic pump for 4 weeks. We found that OTUD1 expression levels were significantly elevated in the kidney tissues of Ang II-treated mice. Otud1 knockout significantly ameliorated Ang II-induced HRD, whereas OTUD1 overexpression exacerbated Ang II-induced kidney damage and fibrosis. Similar results were observed in TCMK-1 cells but not in SV40 MES-13 cells following Ang II (1 μM) treatment. In Ang II-challenged TCMK-1 cells, we demonstrated that OTUD1 bound to CDK9 and induced CDK9 deubiquitination: OTUD1 catalyzed K63 deubiquitination on CDK9 with its Cys320 playing a critical role, promoting CDK9 phosphorylation and activation to induce inflammatory responses and fibrosis in kidney epithelial cells. Administration of a CDK9 inhibitor NVP-2 significantly ameliorated Ang II-induced HRD in mice. This study demonstrates that OTUD1 mediates HRD by targeting CDK9 in kidney epithelial cells, suggesting OTUD1 is a potential target in treating this disease.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2023
·
IF 3.3
Huang Yicheng,Yin Liying,Xu Wenqi,Duan Cuicui,Ma Fumin,Ma Yanli,Li Xiaolei,Li Dan
To develop a rice wine with the fragrance and medicinal ingredients of lilies, aroma-producing yeasts ( Saccharomyces cerevisiae H1, Hansenula sp. H3, and Pichia kudriavzevii H6) were used for the production of lily rice wine. When the amount of lily added was 1.5 g per 10 g glutinous rice, the content of flavonoids and polyphenols reached 55.54 and 67.68 mg/L, respectively. The sensory evaluation suggested that lily rice wine had the highest score for “malty”, “sweety”, and “fruity” when the amount of lily added was 0.5 g per 10 g glutinous rice. The result of temperature-programmed headspace gas chromatography ion mobility spectroscopy (TP-HS-GC-IMS) and headspace solid-phase microextraction gas chromatography–olfactometry-mass spectroscopy (HS-SPME-GC-O-MS) clearly showed that linalool and 3-methyl butanal, the unique aromatic compounds of lily, were only detected in fermented lily rice wine to provide flower fragrance. GC-O and odor activity values (OAVs) showed that 11 compounds including ethyl acetate, 2-methyl-1-propanol, 3-methyl-1-butanol, 2-methyl-1-butanol, 3-methylbutyl acetate, ethyl caprate, phenylethyl alcohol, ethyl caprylate, phenethyl acetate, 2-methoxy-4-vinylphenol and γ-nonanoic lactone were indicated to be the major aroma contributors. Based on the aroma recombination experiments, they were further revealed to be the key aroma-active compounds in lily rice wine. At the same time, multivariate data analysis further revealed the correlation between sensory attributes and volatile organic compounds (VOCs) in lily rice wine. This work provides a lily rice wine with a stronger fruit aroma and higher polyphenol content. Graphical abstract
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Journal of Analysis and Testing
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2018
·Vol. 2
·期 4
·332-341
·
IF 0
Wei Yin Hui,Li Xin Yu,Gao Jie,Liu Jia Jun,Yuan Dan,Yin Bin Cheng,Wang Jian
As an antibiotic, tetracycline has been widely used against bacterial infection or as feed additives for growth promotion, which may lead to the enrichment of antibiotic residues in animals or their products. Therefore, the detection of tetracycline is closely related to human health. The morphological transformation of CoOOH nanoflakes induced by tetracycline was monitored by scanning electron microscope images, dark-field light scattering images and optical spectra, which found that tetracycline could decompose CoOOH nanoflakes into small nanoparticles with low light scattering signal. As a result, the reduced light scattering of CoOOH nanoflakes was proportional to the increased concentration of tetracycline in the range of 1.0–250 μmol/L, and the limit of determination (LOD) was 0.23 μmol/L (3σ/k). Most foreign substances did not interfere in the analysis of tetracycline. Furthermore, the concentration of tetracycline in different milk samples detected with the standard addition method was so low that it accorded with the safety regulation, which was similar to the detection result with high-performance liquid chromatography (HPLC).
研究领域:
分析化学
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纳米技术
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食品安全
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药物分析
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JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 54
·期 7
·5961-5970
·
IF 3.442
Luo Shuai,Qiao Xuan,Wang Qiu-Yu,Zhang Yun-Fei,Fu Ping,Lin Zhi-Dong,Du Fei-Peng,Cheng Chun
To develop low-cost and high-performance self-healing antifogging coatings, polyvinyl alcohol/hydrolyzed poly(styrene-co-maleic anhydride) (PVA/H-PSMA) coatings were prepared from a mixed solution of polyvinyl alcohol (PVA) and hydrolyzed poly(styrene-co-maleic anhydride) (H-PSMA) via spin coating and annealing. The microstructure, antifogging behavior, and self-healing ability were investigated. High transparency and low surface roughness were observed when the PVA/H-PSMA film was coated on glass slides. The PVA/H-PSMA coatings showed excellent antifogging performance in a warm, humid environment. The coatings also performed well when transferred from a refrigerator to room temperature. The antifogging coatings showed desirable water resistance due to strong chemical bonding between PVA and H-PSMA, as well as excellent self-healing ability due to hydrogen bonding self-assembly in the PVA/H-PSMA structure. The coatings still exhibited excellent antifogging effects after 20 scratching-repairing cycles, illustrating their potential use in practical applications.
研究领域:
材料科学
·
表面化学
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FIBERS AND POLYMERS
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2022
·Vol. 23
·期 4
·1040-1049
·
IF 2.347
Jin Liang,Qian Tao,Zhu Tao,Wang Ren-Liang,Chen Wei-Guo,Jiang Hua,Cui Zhi-Hua
Carboxylic acid ester disperse dyes containing an azo pyridone structure have a high molar extinction coefficient, excellent washing color fastness, and simple synthetic methods. Dyes were readily synthesized through a conventional diazo coupling reaction between the reactant ethyl p -aminobenzoate and a coupling component containing an azo pyridone structure. Subsequently, the molecular structures of the target products were characterized by FTIR, mass spectrometry, 1 H NMR, 13 C NMR, UV-vis absorption spectroscopy, and elemental analysis. The synthesized dyes were used to dye poly(ethylene terephthalate) fabric, and their washing and rubbing color fastness properties were tested and compared under different post-treatment methods. Moreover, under the condition of alkali clearing, the relationships among different alkali concentrations and clearing effects were studied to determine the optimal alkali clearing process conditions for dyed fabrics, and the minimum alkali consumption required to obtain the best results was determined. It was found that carboxylic ester disperse dyes containing an azo pyridone structure showed good dyeing performance and alkali-clearable properties on polyester fibers. In addition, in hydrolyzate recovery, BaCl 2 (salting out) can be used instead of acidification to recover the dye hydrolyzate, which has good environmental benefits.
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Nature Communications
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2022
·Vol. 13
·期 1
·1-15
·
IF 17.694
Xia Neng,Jin Dongdong,Pan Chengfeng,Zhang Jiachen,Yang Zhengxin,Su Lin,Zhao Jinsheng,Wang Liu,Zhang Li
The geometric reconfigurations in three-dimensional morphable structures have a wide range of applications in flexible electronic devices and smart systems with unusual mechanical, acoustic, and thermal properties. However, achieving the highly controllable anisotropic transformation and dynamic regulation of architected materials crossing different scales remains challenging. Herein, we develop a magnetic regulation approach that provides an enabling technology to achieve the controllable transformation of morphable structures and unveil their dynamic modulation mechanism as well as potential applications. With buckling instability encoded heterogeneous magnetization profiles inside soft architected materials, spatially and temporally programmed magnetic inputs drive the formation of a variety of anisotropic morphological transformations and dynamic geometric reconfiguration. The introduction of magnetic stimulation could help to predetermine the buckling states of soft architected materials, and enable the formation of definite and controllable buckling states without prolonged magnetic stimulation input. The dynamic modulations can be exploited to build systems with switchable fluidic properties and are demonstrated to achieve capabilities of fluidic manipulation, selective particle trapping, sensitivity-enhanced biomedical analysis, and soft robotics. The work provides new insights to harness the programmable and dynamic morphological transformation of soft architected materials and promises benefits in microfluidics, programmable metamaterials, and biomedical applications.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-10
·
IF 5.705
Zhou Xiu-Jie,Zhang Li-Shun,Song Wen-Fang,Huang Yan-Ping,Liu Zhao-Sheng
An inorganic-organic hybrid monolith incorporated with stellated mesoporous silica nanoparticles (SMSNs) was prepared. Using binary solvents, deep eutectic solvents and room temperature ionic liquids, an SMSN-incorporated poly(butyl methacrylate- co -ethylene glycol dimethacrylate) monolith demonstrated uniform structure with good column permeability. A systematic investigation of preparation parameter was performed, including SMSN content, crosslinking monomer content, and the component of binary solvent. The optimized monoliths were characterized by field emission scanning electron microscopy, transmission electron microscopy, area scanning energy dispersive spectrometry, and nitrogen adsorption. Column performance was tested by separating four groups of analytes (alkylbenzenes, anilines, naphthalenes and phenols) by capillary electrochromatography (CEC). Baseline separation of all analytes was obtained with column efficiencies of up to 266,000 plates m −1 . The performance of the resulting monolith was further investigated in detail by separating mixtures of polycyclic aromatic hydrocarbons (PAHs), nonsteroidal antiinflammatory drugs (NSAIDs), and hydroxybenzoic acid isomers. Compared with the corresponding SMSN-free monolith, the CEC performance was improved by about six times. Successful extraction of PAHs and quinolones (QNs) were also performed using this capillary. Improved extraction efficiency (20.2%) for complex samples, lake water, was also found when the material was applied to solid phase microextraction of fluoranthene.
A poly(butyl methacrylate-co-ethylene glycol dimethacrylate) monolith incorporated with stellated mesoporous silica nanoparticles was prepared. It demonstrated column efficiency up to 266,000 plates m −1 in capillary electrochromatography and ability as solid phase microextraction for organic small molecules with good column permeability.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Pang Wenyang,Cao Yin,Du Shuang,Liang Zuowen,Wang Hongliang,Liu Lingyun
Background Tire wear particles (TWPs) are a major source of micro- and nanoplastics. However, reports on nanoscale tire wear particles, especially regarding their potential hazards to the male reproductive system, remain scarce. Results Here, we examined whether nanoscale tire wear particles impair spermatogenesis and blood-testis barrier integrity through ROS-linked ferroptotic injury. Using a 70-day oral exposure model in pubertal male mice and complementary GC-2 and TM4 cell models, we found that high-dose particle exposure reduced epididymal sperm density, increased sperm abnormalities, disrupted seminiferous epithelial organization, and increased BTB permeability. TWPs shifted metabolomic profiles toward lipid and iron dysregulation, suppressed SLC7A11/GPX4, increased ACSL4 and labile Fe²⁺, and depleted junctional proteins. Ferrostatin-1 reduced lipid peroxidation, iron accumulation, cell death, and junctional protein loss, indicating a functional contribution of ferroptosis. Mechanistically, TWPs first induced total and mitochondrial reactive oxygen species and mitochondrial depolarization, which were restrained by N-acetylcysteine. Downstream, autophagic ferritin degradation amplified ferroptotic injury, as chloroquine or NCOA4 knockdown preserved ferritin, reduced malondialdehyde accumulation, restored GPX4/SLC7A11, and improved viability. NCOA4 depletion did not substantially blunt ROS induction, placing ferritinophagy after redox imbalance. Conclusions Together, these data define a ROS-initiated, NCOA4-dependent ferritinophagy-ferroptosis axis that connects nanoscale TWPs to BTB failure and impaired spermatogenic homeostasis. This work provides a mechanistic basis for future assessment of reproductive health risks associated with TWPs exposure. Graphical abstract
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Scientific Reports
·
2026
·
IF 4.9
Yan Mengqi,Li Chen,Li Cuixian,Zhuang Fan,Zhu Piao,Huang Beijian
To compare sonodynamic therapy (SDT) efficacy mediated by protoporphyrin IX-loaded liposomes (PLP) and heating effects across three non-focused ultrasound (US) platforms under approximately matched geometric-area-normalized intensity ( I MGA ). Three platforms were evaluated using a standardized direct-contact 96-well 4T1-cell model. Acoustic power was measured using a radiation force balance, with I MGA derived from measured power and visible transducer-face area. Cell viability was assessed by CCK-8, intracellular reactive oxygen species (ROS) generation by DCFH-DA-based imaging and flow cytometry, and the heating effect by infrared thermography supplemented with thermocouple measurements. Despite matched I MGA , platforms differed in cytotoxicity and heating. At low I MGA , US-alone cytotoxicity was limited, whereas PLP-dependent viability loss was observed for Platforms A and B. At high I MGA , Platforms A and B showed US-alone cytotoxicity and spatial heterogeneity, whereas Platform C retained PLP-dependent cytotoxicity. PLP + US enhanced intracellular ROS generation across all three platforms. Endpoint temperature was inversely associated with US-alone cell viability at high I MGA for Platforms A and B. At a fixed displayed intensity, measured time-averaged acoustic power scaled approximately with duty cycle. PLP-mediated SDT efficacy remained platform-dependent at approximately matched I MGA . Independent verification of acoustic output and temperature monitoring are essential for reproducible cross-platform interpretation of SDT studies.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Chu Shiqi,Yang Lian,Chi Zhongmei,Ma Jiahui,Wang Huanxiang,Yan Lihe,Li Zhaoye,Liu Guocheng
Dopamine (DA) in bovine serum served as a vital physiological biomarker for evaluating livestock health status and conducting translational biomedical research. Accurate and rapid detection of DA was therefore essential for agricultural breeding monitoring and clinical biological analysis. In this work, a novel Hemin@P 2 Mo 18 nanocomposite with peroxidase-mimicking activity was successfully prepared via a facile, green solvent-free grinding strategy. The introduction of water-soluble H 6 P 2 Mo 18 effectively suppressed the aggregation of free hemin molecules and yielded solid-phase Hemin@P 2 Mo 18 . Relative to natural peroxidases, the synthesized Hemin@P 2 Mo 18 featured convenient recovery and excellent reusability. Given the excellent peroxidase-mimicking activity of Hemin@P 2 Mo 18 , a sensitive and selective colorimetric platform was constructed for DA detection. Moreover, the proposed approach showed high selectivity against common interfering substances and excellent stability under different conditions. It was successfully applied to the quantitative determination of DA in bovine serum samples with satisfactory recovery rates ranging from 96.8% to 100.2%. This study provided a green and efficient solvent-free grinding synthesis strategy for high-performance POM-based nanozymes. It also offered a feasible and practical approach for rapid biomarker detection in complex biological matrices, which showed great application potential in veterinary monitoring and biosensing analysis. Graphical abstract
The schematic representation of the synthesis of Hemin@P 2 Mo 18 and the dual-mode detection of dopamine.
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