Advanced Healthcare Materials
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2025
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IF 9.6
Yibing Shi,Xiaoyi Zhu,Qingyuan Xu,Yanling Wu,Yishuo Sun,Xin Zhao,Yulong Liu,Wei Feng,Youhua Xie,Tianlei Ying,Zhenlin Yang
The oral administration of immunofluorophores has been limited by gastrointestinal degradation. To address this, a novel immunofluorophore (ICGM-B9-FCS), based on a single-domain antibody-dye targeting the CEACAM5 antigen, is coated with trifluoromethyl chitosan (5 mg kg −1 ) for NIR-II bioimaging of colorectal cancer and its distant metastases. ICGM-B9-FCS demonstrates unique advantages over existing intraoperative tumor-guidance bioagents, including resistance to liver metabolism, well-retained bioaffinity (EC 50 = 58.09 nmol L −1 ), enhanced relative quantum yield (up to 12 equivalents), significant redshifts in absorption (from 783 to 957 nm), effective oral delivery to colonic metastases, and in situ exploration of the capillary-lymphatic network transportation. These findings establish a regulatory pathway for modifying immunofluorophores as orally administered bioagents, offering innovative methods for preoperative diagnosis and intraoperative guidance in colorectal cancer.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Leqi Gao,Licheng Ma
Chitosan (CTS), well known for its abundant amine (−NH 2 ) and hydroxyl (-OH) groups have potential application for the adsorption of gold ions. However, its low chemical stability and mechanical strength in acidic solution have barely been explored in gold ions sorption. Herein, cross-linked chitosan microspheres were prepared by pre-crosslinked the amino groups on the chitosan molecular chains with formaldehyde which can efficient and selective gold recovery from gold-bearing leaching solution. The cross-linked chitosan microspheres (CTS-GA) were characterized by FTIR, XPS, SEM and BET, and subsequently applied for gold recovery in an actual gold-bearing mineral leaching solution. The maximum adsorption capacity of CTS-GA for Au(III) can reach 614.08 mg/g (C 0 = 100 mg/L, t = 24 h, pH = 2). The CTS-GA was analyzed by ZETA, XRD, TEM, and XPS after the adsorption of Au(III). The results reveal the adsorption mechanism of CTS-GA on gold ions. At pH 2, the amino groups on CTS-GA become protonated and generate a positive charge that electrostatically attracts the negatively charged AuCl 4 − . This interaction allows the nitrogen atoms to complexation with the gold ions, forming a complex that enhances adsorption. Additionally, the amino groups of CTS-GA facilitate the reduction of Au(III) to Au(0). Compared with chitosan, the formaldehyde pre-crosslinking strategy effectively retains active amine sites, thereby enhancing adsorption capacity and stability. In exploration of a complex metal ion environmenniulat in an actual gold-bearing mineral leaching solution, it was indicated that the cross-linked chitosan microspheres possess a significant gold recovery efficiency of 96.56 % at gold concentration of 15.97 mg/L. The CTS-GA can selectively recovery gold from gold-bearing leaching solution.
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CARBOHYDRATE RESEARCH
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2025
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IF 2.5
Yongsheng Zheng,Kai You,Cuiping Guo,Zhijie Geng,Yuwei Gao,Zhaoming Liu,Dong Yang,Huilong Guo,Jun Huang
We report a hydrogel platform that integrates a natural antimicrobial as part of the network rather than as a releasable additive. Creatine was first grafted onto methacrylated hydroxybutyl chitosan to obtain an injectable, thermoresponsive, and photocurable backbone (HBC_m_Cre). Reuterin (Reu) was subsequently incorporated through dynamic imine (Schiff-base) coupling, functioning as structural crosslinks that both reinforce the network and sustain local antimicrobial activity. 1 H NMR and FTIR confirmed the successful synthesis. The resulting HBC_m_Cre/Reu hydrogel exhibited smooth injectability, rapid photocuring, and stepwise enhancement in mechanical performance (HBC: 0.45 ± 0.09 N, HBC_m_Cre: 1.27 ± 0.08 N, HBC_m_Cre/Reu: 1.96 ± 0.09 N), indicating that the integration of Reu as a network-forming unit significantly strengthens the gel architecture. The system maintained high biosafety (NIH 3T3 viability >95 %, hemolysis <2 % at 10 mg/mL) and showed potent antibacterial activity, achieving >95 % killing of E. coli and S. aureus and effective biofilm disruption. In vivo rat tail-amputation experiments further demonstrated efficient bleeding control, with creatine grafting markedly reducing bleeding time and blood loss, while Reu primarily contributed to mechanical reinforcement and antibacterial protection rather than direct hemostatic enhancement. These findings establish a distinct structural design strategy where a natural metabolite and antimicrobial are co-integrated into a single hydrogel network, providing a robust and multifunctional platform for potential application in infection-resistant wound care.
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CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Qi Dong,Siyu Xiong,Xiongwei Dong,Yihang Tang,Xuan Dai,Peng Chen,Zhaowei Zhang
Current surgical adhesives struggle with slow curing, weak wet adhesion, and a lack of antimicrobial properties, leading to ineffective hemostasis and infection control. We developed a novel light-activated chitosan hydrogel (GTCS) through initiator-free photopolymerization via disulfide ring opening. Unlike toxic acrylate-based systems requiring photoinitiators, this method eliminates cytotoxicity risks in existing photopolymerized biomaterials. The GTCS is dual-functionalized with gallic acid (GA) and α-thioctic acid (TA), enabling fast initiator-free photopolymerization (11 s) under UV light. The optimized GTCS2 hydrogel demonstrates superior adhesion strength (31.40 ± 5.09 kPa) and shows broad-spectrum antibacterial efficacy (>98 % killing against E. coli and S. aureus ) via membrane disruption and ROS generation. Crucially, GTCS2 achieves hemostasis in 32 ± 3.6 s (vs. 158.33 ± 13.05 s for gelatin sponge) with a 70 % reduction in blood loss (0.81 ± 0.16 g vs. 2.62 ± 0.42 g). In infected wounds, it accelerates healing (93.23 % closure at day 14 vs. 86.05 % of commercial 3 M dress) by suppressing bacterial colonization, scavenging pathological ROS, and modulating pro-inflammatory cytokines (TNF-α, IL-6). Integrating injectability, rapid light-triggered curing, robust adhesion, and intrinsic antibacterial/antioxidant activity, this multifunctional platform offers a transformative solution for emergency hemorrhage control and infected wound management.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2025
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IF 4.9
Haohao Zhang,Mingyuan Pang,Min Yang,Zhen Kong,Jiajia Ye,Wensi Li,Yen Leng Pak,Juan An,Koulong Wu,Jibin Song
Chemotherapy, as a primary cancer treatment modality, faces limitations in efficacy and patient tolerance due to non-specific cytotoxicity, drug resistance, and adverse effects. This study develops a multifunctional nanoplatform (DOX-FA-CS-BiO 1 . 5 ) for NIR-Ⅱ photoacoustic imaging-guided visualized synergistic photothermal-chemotherapy, aiming to achieve enhanced therapeutic outcomes. The BiO 1 . 5 component ensures superior photothermal conversion efficiency and photoacoustic imaging performance, while the synergistic platform's high drug loading capacity and pH-responsive controlled release profile significantly improve tumor cell eradication rates. The chitosan-folic acid dual coating enhances biocompatibility and tumor-targeting specificity. Through synergistic effects, this nanoplatform demonstrates superior antitumor efficacy, offering a novel paradigm for constructing integrated platforms for imaging-guided visualized cancer theranostics.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xiangyu Zi,Xiaolong Sun,Fengwei Yin,Jia Gao,Jie Huang,Yingying Zhang,Yao Chen,Yongqian Fu
MOFs represent a promising platform for enzyme immobilization, yet the development of tailored strategies to accommodate both enzyme properties and MOF structures remains a challenge. In this study, we employed chitosanase BCC1 as a model enzyme and integrated Fe 2+ /Mn 2+ ions into the ZIF-8 to construct a bimetallic MOF-based immobilization system (BCC1@Fe/Mn-ZIF-8). Comprehensive characterization by SEM, FTIR, TGA confirmed the successful immobilization and structural integrity of the composite. The immobilized enzyme retained 97 % of its initial activity and exhibited enhanced stability under harsh thermal, pH, and solvent conditions. Notably, BCC1@Fe/Mn-ZIF-8 maintained 117 % activity after 14 days at 4 °C and 72 % residual activity after 14 reuse cycles, indicating excellent storage and reusability. This bimetallic MOF-based strategy offers a robust and efficient approach for enzyme immobilization, with strong potential in industrial biocatalysis.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Shuang Sun,Wen Bao,Huaqin Zhang,Xieraili Maimaitiyiming
Recent advances in flexible electronics technology have heightened demand for safe, long-life energy storage devices. This paper employs a functionally oriented and component-synergistic approach to design and prepare multifunctional hydrogel electrolytes (HEs) for constructing long-life flexible zinc-ion hybrid supercapacitors (ZHICs). The study utilized biomass gelatin as a scaffold, incorporating carboxymethyl chitosan (CMCs) and zwitterionic materials to form a physicochemical dual network. This approach constructed a multi-layered dynamic bonding network, endowing the hydrogel with self-adhesive and self-healing intelligent properties, significantly enhancing its mechanical strength and electrical conductivity. Ultimately, sodium alginate (SA) was incorporated to confer 2D printing adaptability, successfully identifying the optimal formulation composite hydrogel as the printing ink. The introduction of SA enabled the composite hydrogel to form a stable, interpenetrating network with both rigidity and toughness, exhibiting high mechanical properties (toughness is 100 MJ m −3 ), zinc plate interfacial adhesion energy is 13.96 J m −2 , alongside self-healing capabilities upon injury (10 min self-healing rate: 22.3%). Rapid ion-crosslinked HEs formed by immersing composite hydrogels in Zn 2+ solutions exhibit a conductivity of 0.34 S m −1 . The ZIHCs devices assembled from HEs have a thickness of 2 mm, operate stably under certain pressure and temperature variations, and demonstrate stable cycling characteristics exceeding 11,000 cycles. This research provides inspiration for designing flexible hydrogel electrolytes with excellent environmental adaptability.
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ACS Omega
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2026
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IF 5.2
Xintong Zhai,Mengjiao Wang,Wenfei Li,Xinyi Yin,Huiran Qi,Yuejiu Pang,Shupeng Su
Stabilizing Pickering emulsions with highly hydrophilic biopolymers such as chitosan remains challenging due to their strong hydrophilicity. By adjusting the pH to impart minimal or zero charge, these highly hydrophilic particles can form stable Pickering emulsions. However, no consensus has been reached regarding the optimal pH to fabricate stable Pickering emulsions. Hence, this study focused on the narrow pH window near the pKa of chitosan (pH 6.1–6.9) to clarify the optimal pH and the related formation and stability mechanisms. At pKa (pH 6.5), chitosan formed self-aggregated particles with optimal amphiphilicity and moderate surface charge, facilitating robust adsorption at the oil–water interface. The resulting emulsions exhibit remarkably small droplet sizes, good fluidities, high encapsulation capacities, and exceptional stability against creaming, coalescence, flocculation, and centrifugation. In contrast, emulsions prepared at pH below 6.5 suffered from excessive hydrophilicity and poor coalescence stability, while those at pH above 6.5 experienced particle overaggregation and gelation, leading to compromised fluidity and interfacial integrity. This superior performance of Pickering emulsions at the optimal pH was attributed to the optimal hydrophilic–hydrophobic balance and moderate surface charge of the chitosan particles, facilitating smaller droplet formation and uniform particle coverage. This finding is pivotal for future applications of chitosan Pickering emulsions and offers insights for developing food-grade Pickering emulsions with other hydrophilic charged particles.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Hong-Xiang Chu,Run-Ze Shao,Zhi-Wei Zhang,Yue-Han Sun,Ling-Yun Li,Yun-Ze Long,Jun Zhang
Burns and scalds heal slowly due to tissue loss at the wound site and the susceptibility to bacterial infection. Stem cell therapy can compensate for the missing cells at the wound site and promote wound healing. However, the activity of stem cells is easily affected by solvents and high-molecular-weight polymers, which limits the efficacy of stem cell therapy. Here, we developed a compact and portable in situ dual-channel composite electrospinning device. For the first time, this device allowed mesenchymal stem cells (MSCs) to be attached to the surface of polycaprolactone(PCL)/chitosan fibers. The dual-channel design prevents MSCs from contacting the spinning solution directly, thereby overcoming the negative impact of solvents on cell activity and broadening the range of solvents. This advantage is not achievable with conventional electrospinning methods that incorporate MSCs into the spinning precursor solution. The experimental results confirmed that our method combining electrospray and electrospinning enabled uniform dispersion of MSCs among the fibers, and enhanced stem cells survival, promoting their function of stem cells in the wound healing. Further experiments have confirmed that MSCs incorporation endows nanofiber dressings with antibacterial properties and promotes wound healing by inducing the secretion of epidermal growth factor. This combination of stem cell therapy and nanofibers offered a promising new approach for treating burns and scalds, suitable for both outdoor and home emergency handling.
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POLYMER TESTING
·
2026
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IF 6.4
Xiaogang Zhu,Shijian Chen,Yuyu Feng,Rongri Tan,Junhua Yuan,Hu Chen,Yanhui Liu
Macromolecular crowding effects frequently drive phase separation and have a significant impact on a series of biochemical processes in the cell. Owing to the significant differences in the physical mechanisms of the macromolecular crowding effects induced by different macromolecules, revealing the underlying mechanisms is crucial. From this perspective, in this study, an innovative method combining high-resolution magnetic tweezers and a statistical analysis based on the van’t Hoff relation was applied to systematically study the kinetics and thermodynamics of the folding–unfolding transition for an individual DNA hairpin in a crowded environment induced by carboxylated chitosan(CC). More specifically, from the magnetic tweezer-based experiments, the nonlinear dependence of the critical force of the DNA hairpin on the CC concentration in experimental buffer with a fixed pH value was identified. It was found that the critical force of the DNA hairpin first decreased and then increased with increasing CC concentration. Zeta potential measurement indicated that the Zeta potential of the experimental buffer was sharply reduced with increasing CC concentration from 0.05% to 0.4%. However, increasing the CC concentration above 0.4% did not result in obvious Zeta-potential variation, suggesting that entropy contributions in macromolecular crowding effects must dominate over the enthalpy contributions above this value. The van’t Hoff relation was established based on a series of single-molecule experiments performed at a constant stretching force within a temperature range of 275 K to 305 K, and the thermodynamics results suggested that the total free energy change in a 0.8% CC solution, opposite to that in a 0.2% CC solution, is negative. The proposed method provides a viable pathway to quantify the contributions of entropy and enthalpy to macromolecular crowding effects.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Hanchen She,Huiru Zhao,Qilin Guo,Peng Gu,Hua Qiu,Yangyi Sun,Kunlin Chen
Flexible conductive coatings enhance textiles with functions like heating, luminescence, and sensing. However, they tend to break under external forces, limiting long-term use. Here, a waterborne polyurethane containing disulfide bonds and aldehyde groups was synthesized using 2-hydroxyethyl disulfide as a chain extender and vanillin as a chain terminator. The resulting polyurethane was then blended with chitosan-modified Ti 3 C 2 T X MXene nanosheets to create a flexible conductive fabric coating. The innovative fabric coating exhibits outstanding mechanical properties and self-healing performance, driven by a synergistic dual dynamic covalent bonding mechanism. Dynamic disulfide bonds within the polyurethane matrix enable main-chain reconstruction, while reversible Schiff base linkages between vanillin and chitosan enhance interfacial healing efficiency. After being developed into sensors, the coating effectively monitored human joint movements in real time, achieving a sensitivity of 8 once the strain exceeded 50%. Furthermore, due to the abundance of -OH and -NH 2 groups on chitosan-modified MXene, the conductive fabric coating sensor exhibited humidity responsiveness. It displayed a high sensitivity of −71%, a rapid response and recovery time of less than 10 s, and a wide sensing range of 33% to 97% relative humidity, thereby facilitating real-time monitoring of respiratory status during physical activity. This flexible coating holds significant potential for functional flexible electronics.
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Advanced Healthcare Materials
·
2026
·
IF 11
Yi Luo,Na Li,Shijun Lu,Yingchao Shen,Minxuan Han,Feng Zhang,Xue Wang
Wound healing remains a critical clinical challenge due to uncontrolled bleeding, bacterial infections, poor vascularization, and cell migration at damaged tissue sites. To address this challenge, we developed a radially structured, multifunctional biomimetic scaffold. Tussah silk was subjected to physical shearing and alkaline hydrolysis treatment to obtain tussah silk nanofibers (TSn). The TSn, chitosan (CS), and graphene oxide (GO) were then processed into radially aligned TSn/CS/GO scaffolds. These anisotropic composite scaffolds demonstrated not only antibacterial activity and rapid hemostatic properties but also effectively guided cell migration from the wound periphery toward the center. To achieve multifunctionality, including antioxidant activity, enhanced cell migration, and vascularization, the central void of the TSn/CS/GO scaffold was filled with a silk fibroin hydrogel integrated with Puerarin (PUE). In vivo studies using rat models confirmed that the TSn/CS/GO@PUE3 scaffold significantly accelerated wound healing (99.98 ± 0.02%), angiogenesis (the positive expression rates of CD31 and α-SMA were 21.66 ± 0.74% and 41.28 ± 1.09%, respectively), and collagen deposition (75.26 ± 2.10%). This study thus provides a valuable strategy for developing multifunctional biomimetic scaffolds to accelerate wound repair.
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