INDUSTRIAL CROPS AND PRODUCTS
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2021
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IF 5.645
Ming-Zhao Xiao,Rui Sun,Zhi-Yan Du,Wen-Bin Yang,Zhuohua Sun,Tong-Qi Yuan
Cadmium (Cd) contaminant in soil can pollute crops and poison consumers higher in the food chain. Sorghum ( Sorghum bicolor (L.) Moench) is a promising bioenergy crop that can absorb and accumulate Cd from soil while generate renewable biomass resources for bioethanol or other valuable products, and limit waste production by rational utilization of all components. In this study, a sustainable agricultural strategy was presented and six sorghum cultivars grown under field conditions for the phytoremediation of Cd-contaminated agricultural soil were investigated with the goal of comparing their ability to accumulate Cd and their theoretical bioethanol yields (Cd concentration in the soil was 0.25 and 0.96 ppm). Striking differences were found in the Cd accumulation of these sorghum cultivars, and the result indicated that the Cd accumulation in stalks increased with maturity. The total absorbability of Cd ranged from 19.6 to 148 g/ha, and N6G60 sorghum showed the highest Cd accumulation with a concentration of 9.16 ppm in the stalk on a dry basis. The theoretical yield of bioethanol ranged from 5510 to 7510 L/ha. The overall performance of N6G60 and 15016 sorghums surpassed that of other sorghum cultivars because of their stronger capacities for Cd translocation and higher theoretical yields of bioethanol. Considering the experimental results, as well as the potential ethanol yield and growing situations of sorghums, 15016 sorghum was considered the cultivar showing the most promise for future applications.
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Molecular Catalysis
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2021
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IF 5.062
Zijing Wang,Changjuan Mo,Sixuan Xu,Shanshuai Chen,Tiansheng Deng,Wanbin Zhu,Hongliang Wang
The catalytic conversion of carbohydrate to platform chemicals, e.g. lactic acid (LA), is the key to unlock the potential of biomass. In this study, we report that the combination of Ba(OH) 2 and Ca(OH) 2 can efficiently catalyse high-concentration (> 0.5 M) of glucose to relatively high yield of LA (> 40 %) under mild conditions (60 °C). Both glucose conversion and LA yield were sensitive to the pH of the catalytic system. A high loading of Ba(OH) 2 led to a high initial pH of the reaction solution, resulting in serious side reactions. While, a low loading of Ba(OH) 2 led to a quike decrease of OH − and Ba 2+ both of which were continuously consumpted by the formed LA. The combination of Ca(OH) 2 with Ba(OH) 2 could significantly improve LA yield, since Ca(OH) 2 , with limited solubility, could act as a controlled-release catalyst for the real-time supply of OH − and Ca 2+ . OH − was indispensable for glucose isomerization to fructose as well as for fructose retro-aldol reaction to C 3 intermediates. Ca 2+ could combine with LA to form stable calcium lactate, liberating Ba 2+ to catalyse the conversion of C 3 intermediates to LA. Under the preliminary optimized reaction conditions, the yield of LA could be reached to 42.55 % and 38.32 % from 0.5 M and 1 M glucose, respectively. This catalytic system has advantages of mild reaction condition, short reaction time, and capable of conversion of high-concentration substrates, enabling it with a great potential for practical applications.
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ACTA BIOCHIMICA ET BIOPHYSICA SINICA
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2020
·Vol. 52
·期 12
·1420-1426
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IF 2.836
Fei Mingyue,Mao Xudan,Chen Yiyang,Lu Yalan,Wang Lin,Yang Jie,Qiu Juanping,Sun Dongchang
β-Alanine (3-aminopropionic acid) holds great potential in industrial application. It can be obtained through a chemical synthesis route, which is hazardous to the environment. It is well known that l-aspartate-α-decarboxylase (ADC) can convert l-aspartate to β-alanine in bacteria. However, due to the low activity of ADC, industrial production of β-alanine through the green biological route remains unclear. Thus, improving the activity of ADC is critical to reduce the cost of β-alanine production. In this study, we established a dual-fluorescence high-throughput system for efficient ADC screening. By measuring the amount of β-alanine and the expression level of ADC using two different fluorescence markers, we can rapidly quantify the relative activity of ADC variants. From a mutagenesis library containing 2000 ADC variants, we obtained a mutant with 33% increased activity. Further analysis revealed that mutations of K43R and P103Q in ADC significantly improved the yield of β-alanine produced by the whole-cell biocatalysis. Compared with the previous single-fluorescence method, our system can not only quantify the amount of β-alanine but also measure the expression level of ADC with different fluorescence, making it able to effectively screen out ADC variants with improved relative activity. The dual-fluorescence high-throughput system for rapid screening of ADC provides a good strategy for industrial production of β-alanine via the biological conversion route in the future.
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BIOSENSORS & BIOELECTRONICS
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2020
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IF 10.257
Bingfang Wang,Yuanyuan Luo,Lei Gao,Bo Liu,Guotao Duan
Accurate detection of glucose is essential for the diagnosis of diabetes, wherein effective and sensitive biosensors for glucose detection are needed. Here, we fabricated a glucose sensor based on field-effect transistor (FET) with bimetallic nickel-copper metal-organic frameworks (Ni/Cu-MOFs) as its channel layers which were grown in-situ through a simple one-step hydrothermal method and modified with glucose oxidase (GOD) by using glutaraldehyde (GA) as linkers. Due to the synergistic effect of Ni ions and Cu ions in MOFs, the sensor (GOD-GA-Ni/Cu-MOFs-FET) showed good field effect performance and great responses to glucose through enzymatic reactions . It displayed a piecewise linear relationship in the wide range (1 μM–20 mM), and provided high sensitivity (26.05 μAcm −2 mM −1 ) in the low concentration (1–100 μM) and a low detection limit (0.51 μM). The sensor also had these advantages of high specificity, excellent reproducibility, good short-term stability and fast response time. Especially, it is indicated that the Ni/Cu-MOFs-FETs with high performance have the potential to be available sensors, paving the way for the application of bimetallic MOFs in biosensing.
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NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 45
·期 4
·1959-1966
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IF 3.288
Wei Ji,Wenmei Ao,Mengqiu Sun,Chunlai Feng,Yun Wang
The aim of the present work was to develop a novel method integrating two-step aqueous two-phase extraction and temperature-controlled affinity precipitation for the separation and purification of horseradish peroxidase (HRP) from horseradish roots. First, a two-step aqueous two-phase extraction (ATPE) process was used as a primary separation process to remove sugars and pigments, and then a temperature-controlled affinity precipitation process based on a thermosensitive triblock copolymer (PEG113-b-PVBA49-b-PNIPAM105) was exploited as a further purification process to selectively isolate HRP from other impurity proteins. The optimum PEG4000 (0.20, w/w) − (NH4)2SO4 (0.18, w/w) aqueous two-phase system (ATPS) was obtained by studying the liquid–liquid equilibrium of PEG (400/1000/2000/4000) + (NH4)2SO4/K3C6H5O7/K2HPO4 ATPSs and the distribution behaviors of 9 kinds of model compounds in these PEG-salt ATPSs. The successful isolation of HRP from horseradish roots was achieved by the integrated method without having a negative effect on the structure of HRP, and the extraction efficiency and purification factor of HRP reached 84.26 ± 0.15% and 9.15 ± 0.10, respectively. The integrated method shows outstanding separation and purification performances and has good prospects for the industrial production of plant glycoprotein.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-10
·
IF 6.232
Hu Shuisheng,Lin Yuxia,Teng Jing,Wong Wing-Leung,Qiu Bin
A new electrocatalytic biosensor (MOF-74(Cu) NS-CC) based on the in situ deposition of MOF-74(Cu) nanosheet on carbon cloth via a bottom-up synthetic approach in a glass tube was developed. The electrocatalytic activity of the deposited MOF-74(Cu) NS was demonstrated in the oxidation of glucose to gluconate under alkaline conditions. The results revealed that the proposed method of in situ formation of MOF-74(Cu) NS onto a carbon cloth surface in a multi-layer solution is capable to generate a stable MOF-74(Cu) NS-CC electrode with excellent sensing performance. When the as-synthesized MOF-74(Cu) NS-CC was applied directly as the working electrode for glucose sensing, it showed much higher conductivity and redox activity than MOF-74(Cu) NS-GCE. With the potential applied at 0.55 V (vs. Ag/AgCl), this new electrocatalytic biosensor exhibits an excellent linear relationship between current density and concentration of glucose. Moreover, a wide linear range of detection (1.0 to 1000 μM) was observed. The limit of detection was found to be 0.41 μM (S/ N = 3). The response sensitivity is 3.35 mA mM −1 cm −2 when the concentration of glucose is in the range 1–100 μM and 3.81 mA mM −1 cm −2 for the 100–1000 μM concentration range. This study provides a low-cost, easy to prepare, and reproducible methodology for the synthesis of highly redox-active nanomaterials based on the in situ formation of two-dimensional MOF-74(Cu) NS for the development of new electrocatalytic biosensors.
Graphical abstract
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FOOD CHEMISTRY
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2021
·
IF 7.514
Zhihao Xu,Qinzhi Wang,Hui Zhangsun,Shuang Zhao,Yijian Zhao,Li Wang
In this work, we propose a electrochemical enzyme-free glucose sensor by direct growth of conductive Ni/Co bimetal MOF on carbon cloth [Ni/Co(HHTP)MOF/CC] via a facile hydrothermal method. Due to excellent conductivity between Ni/Co(HHTP)MOF and CC, synergic catalytic effect of Ni and Co elements, the Ni/Co(HHTP)MOF/CC not only provides larger surface area and more effective active sites, but also boosts the charge transports and electro-catalytic performance. Under optimized conditions, the Ni/Co(HHTP)MOF/CC shows excellent activity with a linear range of 0.3 μM–2.312 mM, a low detection limit of 100 nM (S/N = 3), a fast response time of 2 s and a high sensitivity of 3250 μA mM −1 cm −2 . Furthermore, the Ni/Co(HHTP)MOF/CC was successfully applied for the detection of glucose in real serum and beverages with competitive performances. This facile and cost-effective method provides a novel strategy for monitoring of glucose in biological and food samples.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2021
·
IF 4.464
Haitao Wang,Juan He,Lei Ge,Zhi Xu,Wei Zhou,Zongping Shao
Although many metal-based compounds demonstrate the ability to detect glucose, limited metal compounds have the desired high conductivity. Metal nitrides with different states of metal elements (M), such as M 0 and M 2+/3+ , can perform excellent conductivity and catalytic activity. In this work, self-supported non-enzymatic electrochemical sensors supported by carbon paper (CP) were synthesized for monitoring glucose in alkaline solutions. The antiperovskites FeNNi 2 Co and FeNNi 3 as electrocatalysts are first reported in the field of the electrochemical sensor, displaying outstanding catalytic activity and high electrical conductivity. Electrochemical tests suggest that the FeNNi 2 Co/CP and FeNNi 3 /CP electrodes perform outstanding sensing abilities toward rapid glucose determination. In addition, the FeNNi 2 Co with multi-metals can display the trimetallic synergic effect and superior performance than the original material of FeNNi 3 . The sensitivity of FeNNi 2 Co/CP electrode can reach level of 3621 and 1141 μA mM −1 cm −2 in the concentration ranges of 2–2789 μM and 2789–7444 μM, respectively. The repeatability of the glucose concentration in the glucose drink measured by the FeNNi 2 Co/CP electrode can achieve 96.2%.
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FUEL PROCESSING TECHNOLOGY
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2020
·
IF 4.982
Chaonan Tao,Lincai Peng,Junhua Zhang,Liang He
Efficient production of fuels and chemicals from lignocellulosic biomass is the main goal of biorefinery processes. Herein, the metal-modified heteropolyacids with tunable Lewis and Brønsted acid sites were designed as the monolithic multifunctional catalyst for valorizing cellulose and lignocellulosic biomass to alkyl levulinate, a versatile platform molecule. Among them, Al-exchanged two protons of H 4 SiW 12 O 40 (Al 2/3 H 2 SiW 12 O 40 ) was found to be uniquely effective. The kinetic behavior describing the conversion of cellulose to ethyl levulinate was investigated to monitor the process, and compared between parent H 4 SiW 12 O 40 and Al 2/3 H 2 SiW 12 O 40 to understand the mechanistic role of Al 3+ . The introduction of Al 3+ substantially facilitated cellulose depolymerization and glucose-to-fructose isomerization , leading to a high rate and product selectivity. The estimated activation energies for cellulose degradation and glucopyraoside alcoholysis decreased by 50.6 kJ/mol and 40.9 kJ/mol, respectively. 50–72% yields of ethyl levulinate were accomplished from cellulosic biomasses such as pine wood, eucalyptus, bamboo, bagasse and Napier grass. Al 2/3 H 2 SiW 12 O 40 allowed cellulose conversion in various linear alcohols producing high-yield alkyl levulinate, and could be reused multiple times with a good stability. This study opens a reliable and promising avenue for one-pot transformation of cellulose and raw lignocellulosic biomass to ester compounds.
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Journal of Chemistry
·
2020
·
IF 1.79
Xiaofang Liu,Zhigang Liu,Qiuyun Zhang,Hongguo Wu,Rui Wang
MIL-101(Fe) was explored for the first time for the catalytic conversion of glucose into lactic acid (LA). The as-synthesized MIL-101(Fe) was successfully characterized, and its higher specific surface area, porosity, and feasible acid properties were confirmed to determine the remarkable catalytic activity in glucose-to-LA conversion (up to 25.4% yield) compared with other catalysts like MIL-101(Cr, Al) and UiO-66(Zr). The reaction parameters including temperature, reaction time, and substrate species as well as catalyst reusability were discussed.
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ANALYST
·
2020
·Vol. 145
·期 15
·5273-5279
·
IF 3.978
Luping Feng,Jiani Yang,Sheng Zhang,Lixiang Zhang,Xi Chen,Pan Li,Yuan Gao,Shujing Xie,Yun Zhang,Hua Wang
A capillary-based fluorimetric analysis method was developed for probing glucose (Glu) in blood using Glu oxidase-anchored gold nanoclusters (GOD–AuNCs) and the ZIF-8 matrix. AuNCs were attached with GOD to be further encapsulated into the ZIF-8 matrix through the protein-mediated formation route. The resulting GOD–AuNCs@ZIF-8 nanocomposites could present the AuNC-improved catalysis of GOD and ZIF-8-improved environmental stability. The ZIF-8-enhanced fluorescence intensity of AuNCs could also be expected. Moreover, a capillary-based fluorometric platform was constructed for sensing Glu by coating the capillaries first with GOD–AuNCs and then the ZIF-8 matrix. Herein, Glu was introduced through the self-driven sampling to trigger the GOD-catalyzed production of hydrogen peroxide, which could induce the fluorescence quenching rationally depending on the Glu concentrations. The developed fluorimetric method could allow for the rapid and simple detection of Glu with the concentrations linearly ranging from 5.0 μM to 2.5 mM. Besides, the feasibility of practical applications was demonstrated by the evaluation of Glu in blood showing the recoveries of 96.2%–103.4%. Importantly, the proposed design of the capillary-based fluorimetric platform by the synergetic combination of catalysis-specific recognition and fluorescence signaling may open a new door toward extensive applications in the biological sensing, catalysis, and imaging fields.
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ACS Applied Bio Materials
·
2020
·
IF 0
Wei Ding,Haibo Liu,Weiwen Zhao,Jie Wang,Linlin Zhang,Yuewei Yao,Cheng Yao,Chan Song
Recently, the development of nanozymes with high catalytic performance is gaining more and more attention due to the ever-growing demands for their practical applications. The elaborate design of its morphology has demonstrated to be an effective approach to improve the performance of these nanozymes. Herein, a hybrid of iron disulfide nanoparticles (FeS2 NPs) encapsulated by two-dimensional (2D) carbon nanosheets (NSs), denoted as FeS2@C NSs, demonstrates both superior peroxidase-like activity and excellent stability. The incorporation of 2D carbon sheets endows the proposed FeS2@C nanozymes with high specific surface area, providing abundant active sites to facilitate the contact with substrates. Moreover, the embedded FeS2 NPs are kept from aggregation due to the encapsulation and confinement of 2D carbon sheets, avoiding the conventional failure of single-component nanozymes. Based on glucose oxidase (GOx) and the elaborately designed FeS2@C nanozymes, a colorimetric method for glucose detection is then developed with excellent simplicity and sensitivity. The detection limit of the sensing platform is as low as 0.19 μM for a glucose assay. More notably, this method can be successfully employed for the glucose assay in some real samples, indicating the great potential of this FeS2@C NS-based nanozymes in the fields of biotechnology and clinical diagnostics.
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