ACTA PHARMACOLOGICA SINICA
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2024
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IF 6.9
Hu You-tian,Liu Zhi-wei,Zhang Tong-hui,Ma Yu-e,He Lei,Zhang Jie,Zhou Yue-yang,Vidal-Puig Antonio,Pan De-jing,Wu Fang
Thymic egress is a crucial process for thymocyte maturation, strictly regulated by sphingosine-1-phosphate lyase (S1PL). Recently, cystathionine γ-lyase (CSE), one of the enzymes producing hydrogen sulfide (H 2 S), has emerged as a vital immune process regulator. However, the molecular connection between CSE, H 2 S and thymic egress remains largely unexplored. In this study, we investigated the regulatory function of CSE in the thymic egress of immune cells. We showed that genetic knockout of CSE or pharmacological inhibition by CSE enzyme inhibitor NSC4056 or D , L -propargylglycine (PAG) significantly enhanced the migration of mature lymphocytes and monocytes from the thymus to the peripheral blood, and this redistribution effect could be reversed by treatment with NaHS, an exogenous donor of H 2 S. In addition, the CSE-generated H 2 S significantly increased the levels of S1P in the peripheral blood, thymus and spleen of mice, suppressed the production of proinflammatory cytokines and rescued pathogen-induced sepsis in cells and in vivo. Notably, H 2 S or polysulfide inhibited S1PL activity in cells and an in vitro purified enzyme assay. We found that this inhibition relied on a newly identified C 203 XC 205 redox motif adjacent to the enzyme’s active site, shedding light on the biochemical mechanism of S1PL regulation. In conclusion, this study uncovers a new function and mechanism for CSE-derived H 2 S in thymic egress and provides a potential drug target for treating S1P-related immune diseases.
研究领域:
免疫学
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生物化学
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药理学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry C
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2025
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IF 5.1
Weijun Deng,Mengke Wang,Jing Hu,Huitao Wen
Discriminative and sensitive detection of low-concentration odorous gases is of great significance for health diagnostics, product quality control and robotics. However, achieving a biomimetic olfactory system in those applications with hydrogel remains a challenge. Here, by learning the mechanism of human olfactory epithelium, we design a flexible and ion-conductive hydrogel based on cellulose as an artificial nose. The hydrogel is soft, flexible, adhesive, self-healing and conductive, enabling the integration to various devices. The artificial nose exhibits high selectivity to H2S over other gas molecules such as NH3, NO2, CO2, CH3CH2OH, CH2O, etc. It has high linear sensitivity (0.34 ppm-1) with a fast response (143 s). More importantly, the sensoring range of this artificial nose moves into human olfactory threshold of H2S. The sensor is operated under low voltage (1 V) and mild temperature (0-25 °C) with sensoring stability for at least 2 weeks. Furthermore, this artificial nose are demonstrated as promising in applications of noninvasive halitosis diagnosis and meat spoilage identification. Our work may inspire odorant sensor development with artificial olfactory system for robotics and other specific applications.
研究领域:
传感器技术
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仿生工程
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材料科学
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分析化学
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健康诊断
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食品质量控制
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机器人技术
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
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IF 4.3
Guo Sun,Ren-Wei-Yang Zhang,Xu-Yang Chen,Yu-Hua Chen,Liang-Hua Zou,Jian Zhang,Ping-Gui Li,Kai Wang,Zhi-Gang Hu
H 2 S plays a crucial role in numerous physiological and pathological processes. In this project, a new fluorescent probe, SG-H 2 S , for the detection of H 2 S, was developed by introducing the recognition group 2,4-dinitrophenyl ether. The combination of rhodamine derivatives can produce both colorimetric reactions and fluorescence reactions. Compared with the current H 2 S probes, the main advantages of SG-H 2 S are its wide pH range (5–9), fast response (30 min), and high selectivity in competitive species (including biological mercaptan). The probe SG-H 2 S has low cytotoxicity and has been successfully applied to imaging in MCF-7 cells, HeLa cells, and BALB/c nude mice. We hope that SG-H 2 S will provide a vital method for the field of biology.
研究领域:
化学
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生物化学
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生物成像
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分析化学
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DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
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2019
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IF 3.743
Zhongchao Wang,Ying Yan,Yini Wang,Fei Tong
Introduction The purpose of the experiment was to survey the therapeutic function of resveratrol (RES)-loaded poly(ethylene glycol)-poly(phenylalanine) (PEG-PPhe) on intestinal ischemia/reperfusion injury (II/RI) via the interaction between CSE/H 2 S and iNOS/NO compared to free RES in diabetic rats. Methods Diabetic rats were pretreated with 20 mg/kg of RES or the RES/PEG-PPhe complex and then subjected to 1 h of ischemia and 3 h of reperfusion. Blood and intestines were collected, intestinal pathological injury was estimated, and the contents of body weight, weights of different tissues, blood glucose, serum insulin, HOMA index, serum nitric oxide (NO) and serum sulfureted hydrogen (H 2 S) were observed. The dry/wet intestine ratios, the activity of superoxide dismutase (SOD); the contents of methane dicarboxylic aldehyde (MDA), glutathione (GSH), H 2 S, and NO; and the concentrations of inducible nitric oxide synthase (iNOS) and cystathionine-γ-lyase (CSE) were observed in the intestinal tissues. Results A significant reduction of weights of different tissues, blood glucose, pathological damage, dry/wet ratios, MDA, NO, iNOS expression and a significant increasement of body weight, serum insulin, HOMA index, SOD, GSH, H 2 S, CSE expression were observed in both treatment groups. However, a greater reduction of weights of different tissues, blood glucose (7.49–13.49 mmol/L for 72 h vs. the control) and pathological damage, iNOS expression, dry/wet ratios (6.14 ± 0.29 vs. 8.51 ± 0.42), MDA (5.01 ± 0.71 nmol vs. 9.98 ± 0.67 nmol), NO (0.52 ± 0.09 μmol vs. 0.99 ± 0.08 μmol in intestinal tissue; 19.29 ± 0.89 μmol vs. 45.23 ± 1.17 μmol in serum) was observed in the RES/PEG-PPhe group relative to the I/R ( P < 0.01 for all); a greater increasement of body weight, serum insulin, HOMA index, SOD (39.79±1.78 U vs. 11.84 ± 1.02 U), GSH (31.25 ± 1.19 mg vs. 10.13 ± 0.64 mg), H 2 S (39.52 ± 1.32 nmol vs. 13.02 ± 1.03 nmol in intestinal tissue; 9.78 ± 0.79 μmol vs. 3.11 ± 0.85 μmol in serum), CSE expression was observed in the RES/PEG-PPhe group relative to the I/R ( P < 0.01 for all). In addition, aminoguanidine (AMI, iNOS inhibitor) reduced I/R injury, and dl -propargylglycine (PAG, CSE inhibitor) increased I/R injury. Conclusions The interaction between CSE/H2S and the iNOS/NO-mediated resveratrol/poly(ethylene glycol)-poly(phenylalanine) complex alleviates intestinal ischemia/reperfusion injuries in diabetic rats.
研究领域:
药理学
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医学
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分子生物学
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糖尿病研究
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产品关联
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2025
·
IF 6.7
Yuying Cai,Guodong Wang,Linlin Dong,Jiaru Yang,Lanlan Guo,Xiaolian Liu,Yingli Yang,Yongli Tang
The chemical interaction between the target gas and adsorbed oxygen species is a critical factor significantly influencing the sensing performance of metal oxide semiconductor (MOS)-based gas sensors. Thus, we propose a design strategy: improving sensing performance by incorporating materials with exceptional catalytic properties into MOS-based sensing materials. High-entropy oxides (HEOs) demonstrate exceptional catalytic activity attributed to the synergistic effects among their constituent elements and have been the focus within catalysis. Accordingly, we proposed preparing HEOs/In 2 O 3 composite nanofibers with varying HEOs loadings via electrospinning. The micro/nanostructure, elemental composition, oxygen vacancies, and other physicochemical properties were characterized. Compared with pure In 2 O 3 sensors, the load of HEOs improved the response value and reduced response time. Especially, the sensor utilizing In 2 O 3 nanofibers loaded with the optimal amount of HEOs exhibited the highest response to 1 ppm H 2 S at 180 °C, capable of detecting concentrations as low as 100 ppb. Furthermore, this sensor exhibited outstanding selectivity and good long-term stability. The changes in physicochemical properties of In 2 O 3 nanofibers, including the surface oxygen vacancy content, acidity/alkalinity and surface catalytic activity, which were induced by the load of HEOs, are the crucial factors underlying the improvement of the sensor's performance in H 2 S detection. These results provide an excellent theoretical reference for the design of MOS-based sensitive materials to detect H 2 S gas.
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产品关联
DOI
高影响因子
Nano Today
·
2022
·
IF 18.962
Kai Yuan,Kai Huang,Yiqi Yang,Yixuan Lin,Yihao Liu,Fupeng Li,Yakun Liang,Haishuang Chang,Yuhui Chen,Tingting Tang,Shengbing Yang
Increasing evidence has emphasized the pivotal role of antibiotic tolerance in the evolution of antibiotic resistance , refractory infection and infection recurrence. Moreover, satisfactory treatment of infection relies highly on early, precise detection and subsequent timely therapeutic interventions. In this study, the phenotype of increased production of H 2 S in antibiotic-tolerant Staphylococcus aureus ( S. aureus ) was identified and proven to be essential to the maintenance of antibiotic tolerance. Hence, a photoacoustic probe for detecting infection as well as an antibiotic enhancer targeting bacterial endogenous H 2 S to eradicate antibiotic-tolerant bacteria was successfully developed based on a bismuth metal-organic framework (Bi-MOF). Bi-MOF achieved early in situ and precise photoacoustic imaging of infection by generating Bi 2 S 3 inside bacteria. Moreover, Bi-MOF enhanced the eradication efficacy of methicillin against tolerant S. aureus by downregulating the intracellular H 2 S levels. Overall, Bi-MOF offers a promising diagnostic tool for early, precise infection diagnosis and a therapeutic strategy for tolerant bacteria-related refractory infection.
研究领域:
纳米技术
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医学诊断
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抗菌治疗
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传染病
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Fanzhi Meng,Tongyao Ju,Siyu Han,Li Lin,Jinglin Li,Kailun Chen,Jianguo Jiang
The biphasic amine solvent based on ionic liquid could effectively improve the efficiency of carbon dioxide capture and regeneration, greatly reduce the energy consumption of regeneration, and hold an excellent application prospect in the field of large-scale biogas upgrading. In this work, a biphasic mixed amine system consisting of Methyl monoethanolamine (MMEA), N-methyldiethanolamine (MDEA), and 1-butyl-3-methylimidazolium tetrafluoroborate (BF) was prepared for the removal of trace components and CO 2 during biogas upgrading and compared with traditional 30 wt% MEA and mixed amine solution of MMEA and MDEA for biogas upgrading. The results showed that H 2 S and NH 3 would chemically react with the biphasic solvent and thus facilitate the removal of trace components, and that the reacted solution would not affect its regeneration characteristics and could basically be recycled at a regeneration temperature of 125 °C. In addition, the phase separation efficiency of the biphasic agent was not affected by the introduction of trace components, and all reaction products except the ionic liquid were enriched in the rich phases and the carbon enrichment rate reached about 98%, which reduced regeneration energy consumption by 44.07% compared to 30 wt% MEA. Finally, the mechanism of phase separation of reaction products and ionic liquids was discussed in the context of quantum chemical calculations, and the importance of intermolecular van der Waals forces in the phase separation process was clarified, and the reasons for the appearance of phase separation phenomena were explained.
研究领域:
化学工程
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环境科学
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能源技术
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产品关联
DOI
高影响因子
BEHAVIOURAL BRAIN RESEARCH
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2020
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IF 2.977
Yuan-Jian Song,Yue Shi,Miao-Miao Cui,Man Li,Xiang-Ru Wen,Xiao-Yan Zhou,He-Qing Lou,Yu-Lan Wang,Da-Shi Qi,Man Tang,Xun-Bao Zhang
Cerebral ischemia/reperfusion (I/R) injury is a leading cause of learning and memory dysfunction. Hydrogen sulfide (H 2 S) has been shown to confer neuroprotection in various neurodegenerative diseases, including cerebral I/R-induced hippocampal CA1 injury. However, the underlying mechanisms have not been completely understood. In the present study, rats were pretreated with SAM/NaHS (SAM, an H 2 S agonist, and NaHS, an H 2 S donor) only or SAM/NaHS combined with CaM (an activator of CaMKII) prior to cerebral ischemia. The Morris water maze test demonstrated that SAM/NaHS could alleviate learning and memory impairment induced by cerebral I/R injury. Cresyl violet staining was used to show the survival of hippocampal CA1 pyramidal neurons. SAM/NaHS significantly increased the number of surviving cells, whereas CaM weakened the protection induced by SAM/NaHS. The immunohistochemistry results indicated that the number of Iba1-positive microglia significantly increased after cerebral I/R. Compared with the I/R group, the number of Iba1-positive microglia in the SAM/NaHS groups significantly decreased. Co-Immunoprecipitation and immunoblotting were conducted to demonstrate that SAM/NaHS suppressed the assembly of CaMKII with the ASK1-MKK3-p38 signal module after cerebral I/R, which decreased the phosphorylation of p38. In contrast, CaM significantly inhibited the effects of SAM/NaHS. Taken together, the results suggested that SAM/NaHS could suppress cerebral I/R injury by downregulating p38 phosphorylation via decreasing the assembly of CaMKII with the ASK1-MKK3-p38 signal module.
研究领域:
神经科学
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药理学
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神经保护
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信号转导
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
·
IF 13.6
Mengzhao Li,Liping Ren,Zheng Gu,Penghao Gao,Wenbo Sun,Xiaole Dong,Futang Liu,Bingquan Wang,Zijian Zhang,Xinpeng Liu,Peiling Gao
By constructing nanofluid system, trace functionalized nanoparticles can significantly enhance the absorption performance of basic liquid. In this work, amino functionalized carbon nanotubes (ACNTs) and carbon nanotubes (CNTs) were introduced into alkaline deep eutectic solvents to build nanofluid systems and used for the dynamic absorption of H 2 S. The experiment results showed that the introduction of nanoparticles can significantly enhance the H 2 S removal performance of original liquid. When performing H 2 S removal experiments, the optimal mass concentrations of ACNTs versus CNTs were 0.05 % and 0.01 %, respectively. The characterization showed that the surface morphology and structure of the nanoparticles unchanged significantly during the absorption-regeneration process. A double mixed gradientless gas-liquid reactor was used to explore the gas-liquid absorption kinetics characteristics of the nanofluid system. It was found that the gas-liquid mass transfer rate increased significantly after the addition of nanoparticles. The highest total mass transfer coefficient of the nanofluid system of ACNTs was increased to more than 400 % of the value before the addition of nanoparticles. The analysis showed that the shuttle effect and hydrodynamic effect of nanoparticles play important role in the process of enhancing gas-liquid absorption, and the amino functionalization enhanced the shuttle effect of nanoparticles significantly.
研究领域:
化学工程
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Junmei Fan,Enrui Wu,Jinlong Dong,Ruitao Zhu,Meiqin Li,Jiangning Gao,Hongfei Han,Liping Ding
Great efforts have been made to detect hydrogen sulfide (H 2 S) due to its involvements in various physiological processes and disease diagnosis. In the present work, a novel dansyl-based derivative ( DBr ) was synthesized and used as a FRET donor, and pyronine Y ( PyY ) was used as a FRET acceptor for constructing a ratiometric fluorescent sensor with assisstance of surfactant. The non-covalent ternary sensor platform could interact with H 2 S, causing an orange to blue-green fluorescence change as the acceptor emission decreased and the donor emission increased. The remarkable ratiometric response empowers the present system a high sensitivity to H 2 S with a detection limit of 110 nM. Moreover, the detection of H 2 S in serum could also be realized, providing the possibility for practical applications.
研究领域:
分析化学
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生物化学
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材料科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Jingying Pan,Xin Li,Rujing Sun,Yi Xu,Zhenghan Shi,Chaobo Dai,Hao Wen,Ray P.S. Han,Qing Ye,Fenni Zhang,Qingjun Liu
Periodontitis , a chronic disease , can result in irreversible tooth loss and diminished quality of life, highlighting the significance of timely periodontitis monitoring and treatment. Meanwhile, hydrogen sulfide (H 2 S) in saliva, produced by pathogenic bacteria of periodontitis, is an important marker for periodontitis monitoring. However, the easy volatility and chemical instability of the molecule pose challenges to oral H 2 S sensing. Here, we report a wearable hydrogel-based radio frequency (RF) sensor capable of in situ H 2 S detection and antibacterial treatment. The RF sensor comprises an agarose hydrogel containing conjugated silver nanoparticles-chlorhexidine (AG-AgNPs-CHL hydrogel) integrated with split-ring resonators . Adhered to a tooth, the hydrogel-based RF sensor enables wireless transmission of sensing signals to a mobile terminal and a concurrent release of the broad-spectrum antibacterial agent chlorhexidine without complex circuits. With the selective binding of the AgNPs to the sulfidion, the RF sensor demonstrates good sensitivity, a wide detection range (2–30 μM), and a low limit of detection (1.2 μM). Compared with standard H 2 S measurement, the wireless H 2 S sensor can distinguish periodontitis patients from healthy individuals in saliva sample tests. The hydrogel-based wearable sensor will benefit patients with periodontitis by detecting disease-related biomarkers for practical oral health management.
研究领域:
生物传感器
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牙科科学
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材料科学
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无线通信
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Zhiming Hou,Xinyu Ren,Zhuangzhuang Sun,Ruoqi An,Mingzhi Huang,Cen Gao,Mengying Yin,Guangxiu Liu,Dengqi He,Hongliang Du,Rongbing Tang
Bacteria-derived hydrogen sulfide (H 2 S) often contributes to the emergence of antibiotic-recalcitrant bacteria, especially persister (a sub-population of dormant bacteria), thus causing the treatment failure of Catheter-associated urinary tract infection (CAUTI). Here, an H 2 S harvester nanosystem to prevent the generation of persister bacteria and disrupt the dense biofilm matrix by the self-adaptive ability of shape-morphing is prepared. The nanosystem possesses a core-shell structure that is composed of liquid metal nanoparticle (LM NP), AgNPs, and immobilized urease. The nanosystem decomposes urea contained in urine to generate ammonia for eliminating bacteria-derived H 2 S. Depending on the oxidative layer of liquid metal, the nanosystem also constitutes a long-lasting reservoir for temporarily storing bacteria-derived H 2 S, when urease transiently overloads or in the absence of urine in a catheter. Depriving H 2 S can prevent the emergence of persistent bacteria, enhancing the bacteria-killing efficiency of Ga 3+ and Ag + ions. Even when the biofilm has formed, the urine flow provides heat to trigger shape morphing of the LM NP, tearing the biofilm matrix. Collectively, this strategy can turn trash (urea) into treasure (H 2 S scavengers and biofilm rippers), and provides a new direction for the antibacterial materials application in the medical field.
研究领域:
纳米技术
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生物医学工程
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材料科学
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微生物学
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传染病学
✓ 已验证
DOI
PubMed
高影响因子
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