ACS Omega
·
2026
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IF 5.2
Zhaobo Hu,Yan Guo,Peishuo Cao,Keyan Wei,Zongjie Ma,Boxin Li,Dong Li,Liang Zhou,Chang-ye Hui
Abstract This work presents an engineered single-plasmid, dual-pigment whole-cell biosensor for simultaneous colorimetric detection of Pb(II) and Hg(II). To address the challenge of signal interference in cocontaminated environmental samples, a Pb(II)-responsive PbrR regulatory module driving indigo biosynthesis (blue, 620 nm) and an Hg(II)-responsive MerR module driving deoxyviolacein biosynthesis (purple, 570 nm) were integrated into Escherichia coli TOP10. The orthogonal decoupling of the two pigment pathways, combined with a unified 10 h induction protocol, enables independent chromogenic readout of both metals from a single cultivation. Dose-response assays afforded a broad linear dynamic range for Pb(II) of 0.977–2000 μM (R2 = 0.9503) with a limit of detection (LOD) of 0.977 μM, alongside nanomolar sensitivity for Hg(II) with a linear range of 0.244–250 nM (R2 = 0.9800) and an LOD of 0.244 nM. A two-dimensional cross-concentration matrix experiment further demonstrated that the two detection channels maintain distinguishable dose-dependent responses under coexposure conditions, with crosstalk remaining within an acceptable range. Robust performance was retained in spiked surface water and seawater matrices, yielding Pb(II) LODs of 0.244 μM (surface water) and 0.488 μM (seawater), and Hg(II) LODs of 0.488 nM (surface water) and 0.977 nM (seawater). These detection limits approach or fall below relevant environmental regulatory thresholds, including the Class III surface-water standard for Hg(II). This self-contained, instrument-free pigment-based approach enables naked-eye discrimination of both metals in a standard microplate assay, offering a low-cost, high-throughput screening platform that complements standard instrumental methods for on-site environmental monitoring and emergency pollution response.
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Inorganic and Nano-Metal Chemistry
·
2026
·
IF 1.7
Heng-Feng Du,Jia-Tian Shang,Hong-Biao Tang,Shi-jie Wang,Xing Li
Functionalized Au nanomaterials have significant potential in colorimetric detection of fatal toxic metals. In this work, a simply colorimetric sensor was designed and prepared for visual detection of Hg(II) ions based on gold nanoparticles (AuNPs) modified by 5-(m-aminophenyl)tetrazole (APT) and characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and UV − vis absorption spectroscopy. The colorimetric sensor exhibited the fine anti-interference and selectivity in an environment with the presence of other interfering ions. The limit of detection (LOD) is 0.6 µmol/L by the naked eye, and 0.21 µmol/L by UV-vis spectroscopy, indicating that the sensor of AuNPs modified with APT (APT@ AuNPs) could be used for the quantitative determination of Hg2+. Based on the above peculiarity, the sensor could be used to detect Hg2+ in real samples.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Min Chen,Jun Wang,Qianbo Zhang,Jinsheng Zhang,Zhiming Chen,Rongguo Sun
Conventional Hg 2+ visual sensors are unsustainable, hindering their practical application for improved water quality and health. In order to address this challenge, herein, N, S co-doped carbon nanodots (NS-CDs) were prepared and well characterized, presented the fluorescent monitoring for Hg 2+ over other metal ions with the limit of detection (LOD) of 0.47 µM. Next, the CDs were successfully modified by thymine without any fluorescence labelling (referred to as T-NS-CDs). The sensitivity to Hg 2+ cloud be noticeable enhanced due to the formation of T-Hg 2+ -T specific base pairs. Accordingly, the LOD was calculated with values as low as 1.56 nM. Furthermore, Hg 2+ could be released and complexed with antidote (meso-2,3-dimercaptosuccinic acid) (DMSA-Hg 2+ ), being the responsible for the reversible interconversion between T-Hg 2+ -T and DMSA-Hg 2+ . Interestingly, the proposed sensing system also applies to the fluorescent sensing for Hg 2+ in tap water with satisfactory recoveries (96.97 %–101.38 %, RSD < 2 %). Thus, by simply combination of elemental doping and surface functionalization, the surface state and functionalities of CDs could be tailorable, endowing the fluorometric sensing towards Hg 2+ in environmental system.
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APPLIED CLAY SCIENCE
·
2021
·
IF 5.467
Penggang Pei,Tao Sun,Yingming Xu,Yuebing Sun
Mercury (Hg) contamination in water and soil is of great concern due to its high toxicity and biomagnification in food chain. In this study, clay mineral montmorillonites (Mt) was thiol-functionalized via 2–mercaptoethylammonium intercalation (ISH–Mt) and 3–mercaptopropyltrimethoxysilane surface grafting (GSH–Mt) for enhancing remediation ability of Hg contamination. Characterization results indicated that surface grafting significantly changed surface morphology and porous structure , with rougher surface, larger specific surface area and more porous structure. The adsorption capacity of Hg 2+ was significantly enhanced by thiol functionalization, being 141.55 and 136.92 mg g −1 for ISH–Mt and GSH–Mt, respectively. Fitting results of pseudo-second-order kinetic models ( R 2 = 0.879– 0.930) and Freundlich isotherms ( R 2 = 0.900– 0.950) indicated that Hg 2+ was adsorbed on heterogeneous surfaces with chemical processes as the rate-limiting step. TCLP-Hg concentrations in soils after applying ISH–Mt and GSH–Mt (0.1%–1% dosage, m/m ) were < 200 μg L −1 (The Leaching Standards of Hazardous Wastes , USEPA). Hg fractionation shifted from mobile fractions (exchangeable and carbonate-bound) to stable forms (organic matter-bound). XPS and Hg L III -edge EXAFS studies revealed that the complexation of –SH toward Hg was the primary mechanism for Hg adsorption and stabilization. These results illustrated that thiol-functionalized Mts prepared by intercalation and surface grafting are promising for the remediation of Hg-contaminated water and soil.
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NEW JOURNAL OF CHEMISTRY
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2021
·Vol. 45
·期 48
·22888-22901
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IF 3.591
Kaiqi Liu,Luminita Marin,Li Xiao,Xinjian Cheng
Multi-component polymers (MCPs) incorporate multiple components into the main chain or pendant group and have special functions. In this work, the small-molecule fluorescent boron-dipyrromethene (BODIPY), with ester groups and/or alkoxyl long chains, was synthesized first. Then, this small-molecule BODIPY and the components containing CC bonds (HD and PD) were reacted via the Heck coupling reaction to obtain macromolecular probes that perform different functions. With the help of ester groups and alkoxyl “tail”, polymers MCP1 and MCP2 were soluble in a variety of water-miscible organic solvents. Moreover, MCP3 and MCP4 were completely soluble in water. These macromolecular probes showed sensitive and selective recognition toward Hg2+/Hg+ because of the introduced components (HD and PD) containing CC bonds. After binding to Hg2+/Hg+, MCP1 and MCP2 demonstrated fluorescence quenching and color changes, which allowed the dual-channel specific detection of Hg2+/Hg+. Compared with the probes containing HD, the probes containing PD showed higher sensitivity, and the good recognition ability caused the detection limit to be as low as 0.32 μM, which may be due to the “molecular wire” mechanism. The small-molecule components with different functional groups were polymerized into fluorescent polymer materials. This methodology endows the polymer probe with good solubility and high sensitivity, and the as-prepared polymers could sense metal ions in aqueous environments.
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Analytical Methods
·
2023
·
IF 3.1
Jun Zhou,Chengyue Zou,Danqun Huo,Chengxiang Chu,Shasha Liu,Mei Yang,Suyi Zhang,Xianfeng Wang,Changjun Hou
Mercury is a heavy metal with extreme toxicity. Thus, it is of significance to develop an effective method for mercury ion detection with high performance. In this study, carbon dots doped with nitrogen and silicon (N,Si/CQDs) were successfully prepared from folic acid and N-[3-(trimethoxysily)propyl]-ethylenediamine. The N,Si/CQDs show an obvious cyan fluorescence of 460 nm with the radiation of 350 nm. The existence of mercury ions induces the fluorescence quenching of N,Si/CQDs due to photoinduced electron transfer, which was applied for the sensitive sensing of Hg(II). More importantly, the practical application of the N,Si/CQD probe was confirmed by measurements of Hg(II) in real samples of lake water, sorghum and rice. In addition, the N,Si/CQD nanoprobe was integrated on a sensing strip for specific detection of Hg(II). Quantitative measurement of Hg(II) was realized by the outstanding linearity between the diameter (or fluorescence intensity) of the fluorescence quenching ring and the concentration of mercury ions. The sensor shows potential for rapid detection with a triple signal readout on-site and represents a larger step towards practical applications.
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Food Analytical Methods
·
2021
·Vol. 14
·期 7
·1345-1358
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IF 3.366
Wei Yihua,Zhang Jinyan,Qiu Shuyan,Huang Qingqing,Yuan Linfeng,Chen Liumeng,Dai Tingcan,Tu Tianhua,Zhang Biaojin,Yan Han,Li Weihong
A simple, rapid, and accurate method for determination of selenocystine (SeCys 2 ), methyl selenocysteine (MeSeCys), selenourea (SeUr), selenite (Se(IV)), selenotmethionine (SeMet), and selenate (Se(VI)) in grain crops was developed, based on the coupling of ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) and ultraviolet irradiation hydride generation atomic fluorescence spectrometry (UV-HG-AFS). The conditions of IP-RP-HPLC-UV-HG -AFS were studied. The conditions of the pretreatment sample, such as types and dosage of proteinases, were investigated. The detection limits (LODs) for six Se species were between 0.77 and 1.77 μg·L −1 . Then, the proposed method has been successfully used to analyze selenium (Se) species in Se-enriched rice paddy ( Oryza sativa L. ), soybean ( Glycine max (Linn.) Merr. ), and sweet potato ( Ipomoea batatas (L.) Lam. ) with foliar application of sodium selenite solution. SeMet was the major Se spices in Se-enriched grain crops, ranged from 77–90% of the total Se contents. The capabilities of Se enrichment were relatively larger differences in three-grain corps as follows: soybean > rice paddy > sweet potato.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Houchao Shan,Shufeng Li,Zhen Yang,Xiaoxia Zhang,Yan Zhuang,Qian Zhu,Di Cai,Peiyong Qin,Jan Baeyens
Triazine-based N-rich covalent organic polymer (TBN-1) was prepared for effective removal and selective detection of Hg 2+ from model solutions. The π-π conjugated structure formed by alternating electron-deficient triazine group and electron-rich benzene group promotes the electron transfer and strong fluorescence. The ultra-high specific surface area and abundant metal complex groups provide excellent adsorption sites for heavy metals. The TBN-1 suspension has obvious fluorescence quenching effect when encountering Hg 2+ even when the solutions are treated with co-ions and humic acid. The merits of TBN-1 are unreservedly manifested when further testing the adsorption kinetics of TBN-1 for Hg 2+ removal. With the maximum adsorption capacity of 1630 mg g −1 , 99.99% of Hg 2+ can be removed within 20 min from a 10 ppm solution using TBN-1. The covalent organic polymer (COP) also exhibits a high stability in a wide range of pH and a significant selectivity for Hg 2+ . About 99.9% Hg 2+ can be adsorbed in acidic, co-ions competitive and humic acid contaminated solutions. The TBN-1 can be also reused for at least 5 cycles with acceptable Hg 2+ adsorption capacity retention. The mechanism for the excellent Hg 2+ adsorption performance of TBN-1 is revealed by coupling physico-chemical model fitting, chemical computation, and experimental characterization, which indicates the coordination and cation-π effects and high electron density of the material to facilitate its complexation with Hg 2+ . This work offers new prospects for the application of triazine-based COPs for environmental remediation.
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CHEMOSPHERE
·
2020
·
IF 5.778
Chao Chen,Jing Kang,Jimin Shen,Shengxin Zhao,Binyuan Wang,Zhonglin Chen,Qian Chen
Polyacrylonitrile fiber was successfully modified with triazine-based dendrimer via grafting method as a promising adsorbent for removal of mercury species from aqueous media. The prepared adsorbent was characterized by elemental analysis, scanning electron microscope , Fourier transform infrared spectroscopy , porous structure analysis and X-ray photoelectron spectroscopy, providing the evidence of successful fabrication. The adsorption conditions were found via varying pH, dosage, coexisting substances, contact time, temperature and concentration. Adsorption performance, described better by the pseudo-second-order kinetics with intraparticle diffusion as rate controlling step and Langmuir isotherm model, indicated a chemisorption process with the maximum Langmuir adsorption amount of 227.64 mg g −1 for mercury ions. Thermodynamically, adsorption of mercury ions was spontaneous and endothermic. Desorption and regeneration experiments demonstrated that it could be reused in five successive adsorption cycles without significant loss of its original performance. Experimental data and density functional theory calculation disclosed the coordination geometries and chelating mechanism between the adsorbent and mercury ions. The proposed study would provide a new prospect for the purification of mercury in aqueous system by functionalizing commercial polyacrylonitrile fiber with dendrimers.
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JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Chen Wang,Guo Lin,Yunhao Xi,Xiteng Li,Zhen Huang,Shixing Wang,Jiling Zhao,Libo Zhang
A novel Zr-MOF adsorbent was synthesized by one-step method and was used to selectively adsorb Hg(II) and Pb(II) from wastewater. The adsorbent can reduce the concentration of mercury and lead to less than the national standard (Hg is 50 μg/L and Pb is 1 mg/L). The entire adsorption process conforms to the pseudo second-order kinetic and Hill isotherm models. At pH 4, the adsorbent removed more than 90% of mercury (to 22 μg/L) and lead (to 0.732 mg/L) in 10 min and 960 min, and the maximum adsorption capacity was 1080 mg/g and 510 mg/g, respectively. In addition, the practical application in wastewater proved that the adsorbent can selectively adsorb Hg(II) and Pb(II) from the mixed ion solution and it still meets national standards for wastewater discharge. The research of repeated experiments proves that the adsorbent can be used repeatedly. The adsorption mechanism was clarified by FTIR, XPS spectroscopy before and after adsorption, and density functional theory (DFT) calculation. In summary, the adsorbent is stable in aqueous solution and has great potential for the efficient removal of trace heavy metal ions (mercury and lead) in future treatment of wastewater.
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FOOD CHEMISTRY
·
2019
·
IF 5.399
Danyu Zhang,Shiwei Yang,Qingfang Ma,Jiannan Sun,Heyong Cheng,Yuanchao Wang,Jinhua Liu
This work establishes a hyphenated methodology coupling HPLC with ICP-MS for simultaneous speciation analysis of arsenic, mercury and lead for the first time. Four arsenicals (As(III), DMA, MMA and As(V)), four mercurials (Hg(II), MeHg, EtHg and PhHg) and three lead compounds (Pb(II), TML and TEL) were simultaneously analyzed within only 8 min with acceptable resolution (2.0–8.2 for As, 1.6–6.1 for Hg and 2.7–4.0 for Pb). The detection limits were 0.036–0.20 for As-species, 0.023–0.041 for Hg-species, and 0.0076–0.14 μg L −1 for Pb-species. The developed method was applied for the measurement of five lotus seed samples, indicating the presence of DMA (19.6–28.2 μg kg −1 ), TML (1.4–2.9 μg kg −1 ), MeHg (1.2–4.8 μg kg −1 ) and EtHg (0.8–2.2 μg kg −1 ). This method provides a promising tool for studying the toxic, metabolic and bioavailable behaviors of arsenic, mercury and lead.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Likang Fu,Shixing Wang,Guo Lin,Libo Zhang,Qiming Liu,Ju Fang,Chenhuinan Wei,Gang Liu
A new MOFs adsorbent was prepared by post-functionalization of UiO-66-NH 2 with 2,5-Dimercapto-1,3,4-thiadiazole and utilized to remove the Hg(II) in water selectively. The UiO-66-types were detected by Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscope (FESEM), Brunauer-Emmett-Teller (BET) and zeta potential instruments. The adsorption properties of the new MOFs adsorbent were investigated by batch experiments. The actual maximum adsorption amount was 670.5 mg/g at the optimal pH of 3. Adsorption kinetic and isotherm models were exceedingly fitted to pseudo-second-order and Langmuir/Dubinin-Radushkevich, respectively. The adsorption process and mode were geared to monolayer and chemisorption, the removal rate was directly proportional to the square of mercury ions concentration. The UiO-66-DMTD adsorbent was easy to be regenerated and the removal rate decreased by only 13.5% after ten consecutive cycles. The results of FTIR, XRD and XPS suggested that the adsorption mechanism lay on the complexation reaction between Hg(II) and thiol/nitrogen-containing groups. Moreover, compared with other competitive metal ions, viz., Zn(II), Co(IV), Ni(II), Cd(II), Mg(II), Fe(III), Ca(II) and Cu(II), the UiO-66-DMTD demonstrated an outstanding selective adsorption for Hg(II). These results manifested that the UiO-66-DMTD was a latent adsorbent for the efficient and selective removal of Hg(II) in wastewater.
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